Click here to switch to the map view.

The map label for this gene is azoR2 [H]

Identifier: 125623265

GI number: 125623265

Start: 391309

End: 391914

Strand: Direct

Name: azoR2 [H]

Synonym: llmg_0397

Alternate gene names: 125623265

Gene position: 391309-391914 (Clockwise)

Preceding gene: 125623264

Following gene: 125623266

Centisome position: 15.47

GC content: 35.97

Gene sequence:

>606_bases
ATGTCTAAACTTCTTGTTGTAAAAGGTCATCCACTCACTGCTGAATATTCACTTTCACTCAAAGGTCTTGATGCTTTCGT
CAAATCTTACAAATCTGCTCATCCAGAAGATGAAATTGAAGAGTTGGATGTCTTTAGTGCTGATATTCCAACTTTGAACA
CAGAATTAGTCAGTGCCATGTTTTCAGGTGAAAATGCAGAACTTACAGCATCACAAAAAGATAAATTGGCTCGTTTTGCT
GGTTTTACAGACCAATTTTTGTCAGCTGACAAAGTTGTCATTGCTAATCCAATGTATAATTTGATGATTCCTGCTGAACT
TAAATCATGGGTTGATACTGTAAACGTTGCTGGTAAAACATTTAAATATACCGCTGAAGGACCAGTTGGTTTAGCAAATG
GCAAAAAAGTCTTACATTTACAAGCCAATGGTGGAGTTTATGGAGCACAAGACCCTGCGACAGTTTATATGTCAGCAATC
TTTAACTTTATCGGTAGCGATTTTAGACAAATTGCTGTTGAAGGACATGCTTATGACCCAGAAAAAACAGAAGAACTTCT
TGCTGAATTTATCAAAAAAGTAGAATTAGAAGCTCAAACATTCTAA

Upstream 100 bases:

>100_bases
CAAATAAATGTTTACATGTGTAAATTTTCACGTACTAAATATATTGAGAATTTAGAAAAGTTTGCTATAATAAAAAATAA
TTTCTATAAAGGAACAAAAT

Downstream 100 bases:

>100_bases
ATAAAAGTCTGTCAGTGAACAGACTTTTTCTTGAATTTACTGACAGAAATCTCAGTAAGTAGCTGTAGGTGTTGTCAGTA
AAAGGAAAGGCTAAAAAATG

Product: NAD(P)H dehydrogenase (quinone)

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase 2; FMN-dependent NADH-azo compound oxidoreductase 2 [H]

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MSKLLVVKGHPLTAEYSLSLKGLDAFVKSYKSAHPEDEIEELDVFSADIPTLNTELVSAMFSGENAELTASQKDKLARFA
GFTDQFLSADKVVIANPMYNLMIPAELKSWVDTVNVAGKTFKYTAEGPVGLANGKKVLHLQANGGVYGAQDPATVYMSAI
FNFIGSDFRQIAVEGHAYDPEKTEELLAEFIKKVELEAQTF

Sequences:

>Translated_201_residues
MSKLLVVKGHPLTAEYSLSLKGLDAFVKSYKSAHPEDEIEELDVFSADIPTLNTELVSAMFSGENAELTASQKDKLARFA
GFTDQFLSADKVVIANPMYNLMIPAELKSWVDTVNVAGKTFKYTAEGPVGLANGKKVLHLQANGGVYGAQDPATVYMSAI
FNFIGSDFRQIAVEGHAYDPEKTEELLAEFIKKVELEAQTF
>Mature_200_residues
SKLLVVKGHPLTAEYSLSLKGLDAFVKSYKSAHPEDEIEELDVFSADIPTLNTELVSAMFSGENAELTASQKDKLARFAG
FTDQFLSADKVVIANPMYNLMIPAELKSWVDTVNVAGKTFKYTAEGPVGLANGKKVLHLQANGGVYGAQDPATVYMSAIF
NFIGSDFRQIAVEGHAYDPEKTEELLAEFIKKVELEAQTF

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=184, Percent_Identity=40.7608695652174, Blast_Score=117, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14

Molecular weight: Translated: 21975; Mature: 21844

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLLVVKGHPLTAEYSLSLKGLDAFVKSYKSAHPEDEIEELDVFSADIPTLNTELVSAM
CCCEEEEECCCCCEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
FSGENAELTASQKDKLARFAGFTDQFLSADKVVIANPMYNLMIPAELKSWVDTVNVAGKT
HCCCCCEEECHHHHHHHHHHCCHHHHHCCCCEEEECCCEEEEECHHHHHHHHHHHCCCCE
FKYTAEGPVGLANGKKVLHLQANGGVYGAQDPATVYMSAIFNFIGSDFRQIAVEGHAYDP
EEEECCCCEEECCCCEEEEEEECCCEECCCCHHHHHHHHHHHHHCCHHHEEEECCCCCCH
EKTEELLAEFIKKVELEAQTF
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKLLVVKGHPLTAEYSLSLKGLDAFVKSYKSAHPEDEIEELDVFSADIPTLNTELVSAM
CCEEEEECCCCCEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
FSGENAELTASQKDKLARFAGFTDQFLSADKVVIANPMYNLMIPAELKSWVDTVNVAGKT
HCCCCCEEECHHHHHHHHHHCCHHHHHCCCCEEEECCCEEEEECHHHHHHHHHHHCCCCE
FKYTAEGPVGLANGKKVLHLQANGGVYGAQDPATVYMSAIFNFIGSDFRQIAVEGHAYDP
EEEECCCCEEECCCCEEEEEEECCCEECCCCHHHHHHHHHHHHHCCHHHEEEECCCCCCH
EKTEELLAEFIKKVELEAQTF
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]