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The map label for this gene is thiI [H]

Identifier: 125623264

GI number: 125623264

Start: 388323

End: 389543

Strand: Direct

Name: thiI [H]

Synonym: llmg_0395

Alternate gene names: 125623264

Gene position: 388323-389543 (Clockwise)

Preceding gene: 125623263

Following gene: 125623265

Centisome position: 15.35

GC content: 37.02

Gene sequence:

>1221_bases
ATGACTGTATTATATGATGAAATCATGGTTCGTTATGGTGAACTTTCAACCAAAGGAAAAAATCGTGGTTACTTTATCAA
TCGTTTGGCAAAAAATATTCGCGAAGTTCTTGCAGATATGCCTGAATTAAAAATTACAGCAGTAAGAGATAGAGCACATA
TTGAACTTAATGGAGCAGATTATGCCGAAGTTTCAAATCGTTTAACAAAAGTTTTTGGAATCCAAAATTTTTCACCTTCA
ATCAAAGTTGAAAAATCAATGCCCATTATAAAAAAAGAAGTTGTAGCACTTTTTAAGGAAATTTATAGCGAAGGGCAGAC
TTTTAAAATTGCTACTAGACGAGCTGACCATAACTTTGAATTAGATTCAACAGAATTAAATATTGCTTTAGGAGATGTTG
TTTTTGATAATTTCTCTTACGCAAAAGTACAAATGAAAAAACCAGATATCACGTTGCGCGTTGAAATTAGACAAGATGCG
ACTTACCTTAGTTTTGAAGATATCAAAGGTGCGGGTGGAATGCCAGTTGGTACGGCAGGACGTGGACATTTGATGCTCTC
AGGTGGAATTGATTCACCAGTTGCAGGTTACTTAGCGTTGAAACGTGGCGTTGAGATTGAAGCTGTTCACTTTGCTAGCC
CTCCTTATACTTCACCAGGTGCTCTAAAAAAAGCCAAGGACTTGGCGGCTAAATTAACTGTCTTTGGCGGAGCAATCACT
TTTATTGAAGTTCCATTTACTGAGATTCAAGAAGAAATAAAAGCTAAAGCTCCACAAGCTTATTTGATGACAATCACGCG
TCGTTTCATGATGCGCGTGGTTGACCGTGTTCGTGAAGAACGAGGCGGGAAAGTAATTATTAATGGAGAATCTTTAGGAC
AAGTTGCTAGTCAGACGCTTGGCTCAATGTCTGCCATTAATGAAGTGACTAATACTCCGGTCATTCGACCAGTGGTAACC
ATGGATAAAAATGAAATTATTGAAATTGCTGAAAAAATAGATACTTTTAATTTATCGATTCTACCATTTGAAGATTGCTG
TACAGTCTTTGCCCCACCTTCTCCAAAAACAAATCCTAAACTTGAAAATTGTATTCAATATGAAAAGAGAATGGATGTTG
AAGGAATGGTTGACCGTGCAGTCAAAGGCATTATGCTTACAACGATTGCTGGCGAAAATTGGGACCAAACCGAAGAAGAA
GAATTTGCTGATTTCTTATAA

Upstream 100 bases:

>100_bases
CATTAAATAAAGAAAATTTGACTGGATTTTTAGGTTAAATTGTTCATTTTTTGAAGTATTTAATGATTAAAAGTATGCAA
AAGAATATAGGAGAATAAAA

Downstream 100 bases:

>100_bases
TATGAAGACTCTGCTTGTTATTGATAAGTAGAGTTTTTTTATTTGATACGAAAACTACGATTAATAGTCTAAACTTGACA
AAGGACTGAAACAAGGATAT

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MTVLYDEIMVRYGELSTKGKNRGYFINRLAKNIREVLADMPELKITAVRDRAHIELNGADYAEVSNRLTKVFGIQNFSPS
IKVEKSMPIIKKEVVALFKEIYSEGQTFKIATRRADHNFELDSTELNIALGDVVFDNFSYAKVQMKKPDITLRVEIRQDA
TYLSFEDIKGAGGMPVGTAGRGHLMLSGGIDSPVAGYLALKRGVEIEAVHFASPPYTSPGALKKAKDLAAKLTVFGGAIT
FIEVPFTEIQEEIKAKAPQAYLMTITRRFMMRVVDRVREERGGKVIINGESLGQVASQTLGSMSAINEVTNTPVIRPVVT
MDKNEIIEIAEKIDTFNLSILPFEDCCTVFAPPSPKTNPKLENCIQYEKRMDVEGMVDRAVKGIMLTTIAGENWDQTEEE
EFADFL

Sequences:

>Translated_406_residues
MTVLYDEIMVRYGELSTKGKNRGYFINRLAKNIREVLADMPELKITAVRDRAHIELNGADYAEVSNRLTKVFGIQNFSPS
IKVEKSMPIIKKEVVALFKEIYSEGQTFKIATRRADHNFELDSTELNIALGDVVFDNFSYAKVQMKKPDITLRVEIRQDA
TYLSFEDIKGAGGMPVGTAGRGHLMLSGGIDSPVAGYLALKRGVEIEAVHFASPPYTSPGALKKAKDLAAKLTVFGGAIT
FIEVPFTEIQEEIKAKAPQAYLMTITRRFMMRVVDRVREERGGKVIINGESLGQVASQTLGSMSAINEVTNTPVIRPVVT
MDKNEIIEIAEKIDTFNLSILPFEDCCTVFAPPSPKTNPKLENCIQYEKRMDVEGMVDRAVKGIMLTTIAGENWDQTEEE
EFADFL
>Mature_405_residues
TVLYDEIMVRYGELSTKGKNRGYFINRLAKNIREVLADMPELKITAVRDRAHIELNGADYAEVSNRLTKVFGIQNFSPSI
KVEKSMPIIKKEVVALFKEIYSEGQTFKIATRRADHNFELDSTELNIALGDVVFDNFSYAKVQMKKPDITLRVEIRQDAT
YLSFEDIKGAGGMPVGTAGRGHLMLSGGIDSPVAGYLALKRGVEIEAVHFASPPYTSPGALKKAKDLAAKLTVFGGAITF
IEVPFTEIQEEIKAKAPQAYLMTITRRFMMRVVDRVREERGGKVIINGESLGQVASQTLGSMSAINEVTNTPVIRPVVTM
DKNEIIEIAEKIDTFNLSILPFEDCCTVFAPPSPKTNPKLENCIQYEKRMDVEGMVDRAVKGIMLTTIAGENWDQTEEEE
FADFL

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=355, Percent_Identity=28.4507042253521, Blast_Score=134, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 45224; Mature: 45093

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLYDEIMVRYGELSTKGKNRGYFINRLAKNIREVLADMPELKITAVRDRAHIELNGAD
CEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEECCCC
YAEVSNRLTKVFGIQNFSPSIKVEKSMPIIKKEVVALFKEIYSEGQTFKIATRRADHNFE
HHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
LDSTELNIALGDVVFDNFSYAKVQMKKPDITLRVEIRQDATYLSFEDIKGAGGMPVGTAG
CCCCEEEEEEEEEEECCCCEEEEEEECCCEEEEEEEECCCEEEEHHHCCCCCCCCCCCCC
RGHLMLSGGIDSPVAGYLALKRGVEIEAVHFASPPYTSPGALKKAKDLAAKLTVFGGAIT
CCEEEEECCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEE
FIEVPFTEIQEEIKAKAPQAYLMTITRRFMMRVVDRVREERGGKVIINGESLGQVASQTL
EEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHH
GSMSAINEVTNTPVIRPVVTMDKNEIIEIAEKIDTFNLSILPFEDCCTVFAPPSPKTNPK
HHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEEHHHHHCEECCCCCCCCCC
LENCIQYEKRMDVEGMVDRAVKGIMLTTIAGENWDQTEEEEFADFL
HHHHHHHHHHCCHHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHCC
>Mature Secondary Structure 
TVLYDEIMVRYGELSTKGKNRGYFINRLAKNIREVLADMPELKITAVRDRAHIELNGAD
EEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEECCCC
YAEVSNRLTKVFGIQNFSPSIKVEKSMPIIKKEVVALFKEIYSEGQTFKIATRRADHNFE
HHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
LDSTELNIALGDVVFDNFSYAKVQMKKPDITLRVEIRQDATYLSFEDIKGAGGMPVGTAG
CCCCEEEEEEEEEEECCCCEEEEEEECCCEEEEEEEECCCEEEEHHHCCCCCCCCCCCCC
RGHLMLSGGIDSPVAGYLALKRGVEIEAVHFASPPYTSPGALKKAKDLAAKLTVFGGAIT
CCEEEEECCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEE
FIEVPFTEIQEEIKAKAPQAYLMTITRRFMMRVVDRVREERGGKVIINGESLGQVASQTL
EEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHH
GSMSAINEVTNTPVIRPVVTMDKNEIIEIAEKIDTFNLSILPFEDCCTVFAPPSPKTNPK
HHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEEHHHHHCEECCCCCCCCCC
LENCIQYEKRMDVEGMVDRAVKGIMLTTIAGENWDQTEEEEFADFL
HHHHHHHHHHCCHHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]