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The map label for this gene is apbE [H]

Identifier: 125623257

GI number: 125623257

Start: 379821

End: 380798

Strand: Direct

Name: apbE [H]

Synonym: llmg_0388

Alternate gene names: 125623257

Gene position: 379821-380798 (Clockwise)

Preceding gene: 125623256

Following gene: 125623260

Centisome position: 15.02

GC content: 32.82

Gene sequence:

>978_bases
ATGAAATCTTTAGAAAAAAAAGCGGCAAACAAAATTACAAAATCTTATCGAGCATTAGGAACAGTTATTGATTTAACAAT
CTATGGTTGCCAAGACGAAGGAATTTTAGACCAAAGTTATCAATTAATCAAATATTATGAAGATATTTTTACTGTAAATC
GAGAAAAATCTGAATTAATGACTGTTAATCAGATGGCAGGAATTGAAAGTGTACAAGTTACTGAACCTGTCTATCAACTG
ACAAAAATTTCCTTAGAAAAAAGTAAAGAACATTTCGGATTTAATGCAGCAATTGGGCCTTTAGTTAAACTTTGGCACAT
TGGTTTTTCAGATGCTAAAGTACCAAGCCAAGCAGAAATCTCTGAAAAACTAAAACTTATCAATCCTGATGAGATTGTTT
TAAAGGATGAAGAATTTTCAATATTTCTTCCTAAAAAAGAAATGGAAATTGATCTAGGGGCAATAGCAAAAGGTTATATT
GCTGATCGGATCAAGGATTTTTGGAGGGCTTATGGCGTCTCGTCAGGCATCATTAATCTAGGCGGAAATCTTCTATTAAT
GGGTGAAGCACCCCATCAGACAAATAAAAAATGGCGCGTTGGAGTTAAAAATCCACTAAGTAGTCGCAAAGAACCTATTT
TACAGATTTTGACTGAGGAAAGTTCAGTTGTAACCTCAGGAATTGCTGAGCGTTATTTAAAAGTCAATAATAAAAGTTAT
CATCATATTATTGACCCAGAAACAGGTTATCCTCAAACCAATTCACTTGCAAGTGTCACGGTCTTATCGAGAAAATCCAT
TGATGGAGAGATTGAAACGAGTAGATTATTTTTTGCTGACGGACCAATTGAAAATTGGCTGATAGAGCATCCAGAAGTTT
TTGGAGCAATCTTTATTAGTAAGAATAAAAAGATAAAAACGGTTGGTATTCCTAAAAATCAAATATATATTGCTGATAAA
AGTTTTGAATTTGAATAA

Upstream 100 bases:

>100_bases
AAGAAGTGAATGAGCCTGAAATATCAGCCTATACTCAATTTCTTGAGGAAGATTTGTGGCCCGATGATATTATGGACTCA
AAAGGGACTTTTGAAAACGA

Downstream 100 bases:

>100_bases
GAAAACAAGAGCCTGTCAGTAATTTTGAAGATATAAATTTACTGACAGAAATAAAAAAACCGCCAAAAGCGGTTTTTATT
TTTTATTTCATCGTTGGGAA

Product: thiamine biosynthesis lipoprotein apbE precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MKSLEKKAANKITKSYRALGTVIDLTIYGCQDEGILDQSYQLIKYYEDIFTVNREKSELMTVNQMAGIESVQVTEPVYQL
TKISLEKSKEHFGFNAAIGPLVKLWHIGFSDAKVPSQAEISEKLKLINPDEIVLKDEEFSIFLPKKEMEIDLGAIAKGYI
ADRIKDFWRAYGVSSGIINLGGNLLLMGEAPHQTNKKWRVGVKNPLSSRKEPILQILTEESSVVTSGIAERYLKVNNKSY
HHIIDPETGYPQTNSLASVTVLSRKSIDGEIETSRLFFADGPIENWLIEHPEVFGAIFISKNKKIKTVGIPKNQIYIADK
SFEFE

Sequences:

>Translated_325_residues
MKSLEKKAANKITKSYRALGTVIDLTIYGCQDEGILDQSYQLIKYYEDIFTVNREKSELMTVNQMAGIESVQVTEPVYQL
TKISLEKSKEHFGFNAAIGPLVKLWHIGFSDAKVPSQAEISEKLKLINPDEIVLKDEEFSIFLPKKEMEIDLGAIAKGYI
ADRIKDFWRAYGVSSGIINLGGNLLLMGEAPHQTNKKWRVGVKNPLSSRKEPILQILTEESSVVTSGIAERYLKVNNKSY
HHIIDPETGYPQTNSLASVTVLSRKSIDGEIETSRLFFADGPIENWLIEHPEVFGAIFISKNKKIKTVGIPKNQIYIADK
SFEFE
>Mature_325_residues
MKSLEKKAANKITKSYRALGTVIDLTIYGCQDEGILDQSYQLIKYYEDIFTVNREKSELMTVNQMAGIESVQVTEPVYQL
TKISLEKSKEHFGFNAAIGPLVKLWHIGFSDAKVPSQAEISEKLKLINPDEIVLKDEEFSIFLPKKEMEIDLGAIAKGYI
ADRIKDFWRAYGVSSGIINLGGNLLLMGEAPHQTNKKWRVGVKNPLSSRKEPILQILTEESSVVTSGIAERYLKVNNKSY
HHIIDPETGYPQTNSLASVTVLSRKSIDGEIETSRLFFADGPIENWLIEHPEVFGAIFISKNKKIKTVGIPKNQIYIADK
SFEFE

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=242, Percent_Identity=26.0330578512397, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 36621; Mature: 36621

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLEKKAANKITKSYRALGTVIDLTIYGCQDEGILDQSYQLIKYYEDIFTVNREKSELM
CCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHE
TVNQMAGIESVQVTEPVYQLTKISLEKSKEHFGFNAAIGPLVKLWHIGFSDAKVPSQAEI
EHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHH
SEKLKLINPDEIVLKDEEFSIFLPKKEMEIDLGAIAKGYIADRIKDFWRAYGVSSGIINL
HHHHEECCCCEEEEECCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEC
GGNLLLMGEAPHQTNKKWRVGVKNPLSSRKEPILQILTEESSVVTSGIAERYLKVNNKSY
CCCEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCE
HHIIDPETGYPQTNSLASVTVLSRKSIDGEIETSRLFFADGPIENWLIEHPEVFGAIFIS
EEEECCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEEECCCHHHHHCCCCHHEEEEEEE
KNKKIKTVGIPKNQIYIADKSFEFE
CCCEEEEECCCCCEEEEECCCCCCC
>Mature Secondary Structure
MKSLEKKAANKITKSYRALGTVIDLTIYGCQDEGILDQSYQLIKYYEDIFTVNREKSELM
CCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHE
TVNQMAGIESVQVTEPVYQLTKISLEKSKEHFGFNAAIGPLVKLWHIGFSDAKVPSQAEI
EHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHH
SEKLKLINPDEIVLKDEEFSIFLPKKEMEIDLGAIAKGYIADRIKDFWRAYGVSSGIINL
HHHHEECCCCEEEEECCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEC
GGNLLLMGEAPHQTNKKWRVGVKNPLSSRKEPILQILTEESSVVTSGIAERYLKVNNKSY
CCCEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCE
HHIIDPETGYPQTNSLASVTVLSRKSIDGEIETSRLFFADGPIENWLIEHPEVFGAIFIS
EEEECCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEEECCCHHHHHCCCCHHEEEEEEE
KNKKIKTVGIPKNQIYIADKSFEFE
CCCEEEEECCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]