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The map label for this gene is murF [H]

Identifier: 125623231

GI number: 125623231

Start: 348927

End: 350249

Strand: Direct

Name: murF [H]

Synonym: llmg_0361

Alternate gene names: 125623231

Gene position: 348927-350249 (Clockwise)

Preceding gene: 125623230

Following gene: 125623232

Centisome position: 13.79

GC content: 35.15

Gene sequence:

>1323_bases
ATGAAACTCACAATTCACGAAATCGCTCAAGTCGTTGGCGCAAAAAATGATTGGTCGCAACTGGCAGATTTGTCAGTAAA
TAAAATTGAATTTGACAGCCGTTTAATTGAAAAAGGAGATATCTTTTTGCCACTCAAAGGGGCACGATATGGTCATGATT
TCATCGAGATTGCTTTTGACAATGGAGCAATAATTAGCTTTTCAGAAAAAGAAGTTGAACAAGCTCATCTTTTGGTTGAT
GATAATCTAGCAGCTTTCCAAACATTAGCAAAATATTATCTGGAAAAAACAAAAGTTCCAGTTATTGCGGTGACAGGCTC
AAATGGTAAAACAACCACCAAAGATATGATTGCTGCTGTTTTATCTAAAAAATATAAAACCTATAAAACACAAGGAAATC
ATAATAACGAAATTGGATTGCCTTACACAATTTTACACATGCCAGAGGATACTGAAAAATTAGTATTAGAAATGGGAATG
GACCACCCTGGCGATATTGATTTTCTCTCACAACTAGCTCAACCTGAACTAGCTCTTATTACCTTAATTGGTGAAGCTCA
TCTTGAATTTATGGGTAGTCGTGAAAATATTGCAAAAGGTAAAATGGGTATTACTGCTGGTCTACATGGTGAATTAATTG
CCCCTGCTGACCCAATTATTAATGCTTTTATTCCAGAAAATCAAAAAATCAGTCGTTTTGGTTTGCCCGGTGAAGATTTA
TTCATTACAAAATTAATTGAGCACAAAGAAAGATTGACTTTTGAAACTAATTTTTTAGATGAATCAATAACGATTCCAGT
TCCTGGTAAATATAATGCAACGAATGCTATGTTAGCTGCTTATGTTGGCTTACATTATGGATTGACAGAAGCTGAAATCA
ACAAAGCTTTGAAAGAAGTTGAACTGACCAGAAATCGGACAGAGTGGAAAAAATCTAAAAATGGAGCTGACCTTTTAAGC
GACGTCTATAATGCCAATCCAACAGCTATGCGCCTGATTTTGGAAACTTTCCAAGCCATTCCTAAAAATGAAAATGGTCG
AAAAATCGCAGTTCTTGCTGACATGTTAGAACTCGGACCAACAGCTGCTCAACTTCATAAAGAGGTGCTGAAATCGATTG
ATTTCACAAAAATTGATAAAGTCTATCTTTATGGCGAAATGATGAAAAATTTGGCAGATATTTCGACTGATAAACCTGTC
AGCTATTTTACAGATTTGGATTTACTGACAGAAAGTCTGTCAGCAGATTTGAAACCAGCAGACCAAGTTTTATTCAAGGG
TTCAAACATCATGAAACTTTCAGAAGTTGTTGAAAAATTATAA

Upstream 100 bases:

>100_bases
ATAACCGCAGATAGGGTTGGTTTCATTCGGTCCTAATAAATTGAAAGCCAGTCACACGAAACGTGTTTCATCTGGCTGGC
TGTGTTATAATGGAGTCACT

Downstream 100 bases:

>100_bases
TGACAAAAAAGTACTGACAAGAGTCGGTACTTTTTAGTTTTGTTAAATTGTCAGAAAAATACAATAATATTCTTGACATT
AGATTTTCGAAGAAGTATAA

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6- diaminopimelate--D-alanyl-D-alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MKLTIHEIAQVVGAKNDWSQLADLSVNKIEFDSRLIEKGDIFLPLKGARYGHDFIEIAFDNGAIISFSEKEVEQAHLLVD
DNLAAFQTLAKYYLEKTKVPVIAVTGSNGKTTTKDMIAAVLSKKYKTYKTQGNHNNEIGLPYTILHMPEDTEKLVLEMGM
DHPGDIDFLSQLAQPELALITLIGEAHLEFMGSRENIAKGKMGITAGLHGELIAPADPIINAFIPENQKISRFGLPGEDL
FITKLIEHKERLTFETNFLDESITIPVPGKYNATNAMLAAYVGLHYGLTEAEINKALKEVELTRNRTEWKKSKNGADLLS
DVYNANPTAMRLILETFQAIPKNENGRKIAVLADMLELGPTAAQLHKEVLKSIDFTKIDKVYLYGEMMKNLADISTDKPV
SYFTDLDLLTESLSADLKPADQVLFKGSNIMKLSEVVEKL

Sequences:

>Translated_440_residues
MKLTIHEIAQVVGAKNDWSQLADLSVNKIEFDSRLIEKGDIFLPLKGARYGHDFIEIAFDNGAIISFSEKEVEQAHLLVD
DNLAAFQTLAKYYLEKTKVPVIAVTGSNGKTTTKDMIAAVLSKKYKTYKTQGNHNNEIGLPYTILHMPEDTEKLVLEMGM
DHPGDIDFLSQLAQPELALITLIGEAHLEFMGSRENIAKGKMGITAGLHGELIAPADPIINAFIPENQKISRFGLPGEDL
FITKLIEHKERLTFETNFLDESITIPVPGKYNATNAMLAAYVGLHYGLTEAEINKALKEVELTRNRTEWKKSKNGADLLS
DVYNANPTAMRLILETFQAIPKNENGRKIAVLADMLELGPTAAQLHKEVLKSIDFTKIDKVYLYGEMMKNLADISTDKPV
SYFTDLDLLTESLSADLKPADQVLFKGSNIMKLSEVVEKL
>Mature_440_residues
MKLTIHEIAQVVGAKNDWSQLADLSVNKIEFDSRLIEKGDIFLPLKGARYGHDFIEIAFDNGAIISFSEKEVEQAHLLVD
DNLAAFQTLAKYYLEKTKVPVIAVTGSNGKTTTKDMIAAVLSKKYKTYKTQGNHNNEIGLPYTILHMPEDTEKLVLEMGM
DHPGDIDFLSQLAQPELALITLIGEAHLEFMGSRENIAKGKMGITAGLHGELIAPADPIINAFIPENQKISRFGLPGEDL
FITKLIEHKERLTFETNFLDESITIPVPGKYNATNAMLAAYVGLHYGLTEAEINKALKEVELTRNRTEWKKSKNGADLLS
DVYNANPTAMRLILETFQAIPKNENGRKIAVLADMLELGPTAAQLHKEVLKSIDFTKIDKVYLYGEMMKNLADISTDKPV
SYFTDLDLLTESLSADLKPADQVLFKGSNIMKLSEVVEKL

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=442, Percent_Identity=31.6742081447964, Blast_Score=164, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48927; Mature: 48927

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTIHEIAQVVGAKNDWSQLADLSVNKIEFDSRLIEKGDIFLPLKGARYGHDFIEIAFD
CEEEHHHHHHHHCCCCCHHHHHHCCCCEEEHHHHHHCCCCEEEEECCCCCCCCEEEEEEC
NGAIISFSEKEVEQAHLLVDDNLAAFQTLAKYYLEKTKVPVIAVTGSNGKTTTKDMIAAV
CCEEEEECHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHH
LSKKYKTYKTQGNHNNEIGLPYTILHMPEDTEKLVLEMGMDHPGDIDFLSQLAQPELALI
HHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHCCCCEEEE
TLIGEAHLEFMGSRENIAKGKMGITAGLHGELIAPADPIINAFIPENQKISRFGLPGEDL
EEHHHHHHHHHCCCCHHCCCCCCEEECCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCHH
FITKLIEHKERLTFETNFLDESITIPVPGKYNATNAMLAAYVGLHYGLTEAEINKALKEV
HHHHHHHHHHHCEEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
ELTRNRTEWKKSKNGADLLSDVYNANPTAMRLILETFQAIPKNENGRKIAVLADMLELGP
HHHCCHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCC
TAAQLHKEVLKSIDFTKIDKVYLYGEMMKNLADISTDKPVSYFTDLDLLTESLSADLKPA
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCH
DQVLFKGSNIMKLSEVVEKL
HHHHHCCCCHHHHHHHHHHC
>Mature Secondary Structure
MKLTIHEIAQVVGAKNDWSQLADLSVNKIEFDSRLIEKGDIFLPLKGARYGHDFIEIAFD
CEEEHHHHHHHHCCCCCHHHHHHCCCCEEEHHHHHHCCCCEEEEECCCCCCCCEEEEEEC
NGAIISFSEKEVEQAHLLVDDNLAAFQTLAKYYLEKTKVPVIAVTGSNGKTTTKDMIAAV
CCEEEEECHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHH
LSKKYKTYKTQGNHNNEIGLPYTILHMPEDTEKLVLEMGMDHPGDIDFLSQLAQPELALI
HHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHCCCCEEEE
TLIGEAHLEFMGSRENIAKGKMGITAGLHGELIAPADPIINAFIPENQKISRFGLPGEDL
EEHHHHHHHHHCCCCHHCCCCCCEEECCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCHH
FITKLIEHKERLTFETNFLDESITIPVPGKYNATNAMLAAYVGLHYGLTEAEINKALKEV
HHHHHHHHHHHCEEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
ELTRNRTEWKKSKNGADLLSDVYNANPTAMRLILETFQAIPKNENGRKIAVLADMLELGP
HHHCCHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCC
TAAQLHKEVLKSIDFTKIDKVYLYGEMMKNLADISTDKPVSYFTDLDLLTESLSADLKPA
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCH
DQVLFKGSNIMKLSEVVEKL
HHHHHCCCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]