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The map label for this gene is ddl [H]

Identifier: 125623230

GI number: 125623230

Start: 347614

End: 348663

Strand: Direct

Name: ddl [H]

Synonym: llmg_0360

Alternate gene names: 125623230

Gene position: 347614-348663 (Clockwise)

Preceding gene: 125623229

Following gene: 125623231

Centisome position: 13.74

GC content: 36.38

Gene sequence:

>1050_bases
ATGTCAAAAGAAACATTAATTTTGTTGTATGGTGGTCGTTCTGCTGAGCGAGAAGTATCAGTTTTATCAGCAGAATCAGT
GATGAGAGCTGTCAATTATTCTCGTTTTATTGTGAAAACTTACTTTATCACTAAGGGTGGTGAATTCATAAAAACACAAG
AATTTACTGACAGACCTGCTGAAGAAGAAAAATTACTGACAAACGATTTGGCAGAGTCTTATCCAAAGATTTCACCTGCG
GCAATTTATGAAAAAGATGCTGTTGTATTTCCAGTTTTACATGGTCCAATGGGTGAAGACGGCTCTATTCAAGGATTTTT
AGAAATTTTGCGTTTGGCATATGTCGGACCAAATATTTTATCTGCCTCAAGTACAATGGATAAATTATTAGCAAAACATG
TTTTTGAAGCTGTTGGTGTGCCACAAGTTCCTTATGTTGCCGCTTTTGCTGACGAAAATCAAGTGGAGATTGCTCAAGAA
GTCGTCGAAAAATTAGATTTCCCAGTTTTTGTAAAACCAGCGAACATGGGTTCTAGTGTGGGGATTTCAAAAGTTGATGA
TTTAGCAGATCTTCAACCAGCCTTGAGTGAAGCTTACAAATATGATAACCGTGTGGTCATTGAACAAGGGGTGGATGCGC
GTGAAATCGAGTGTGCTGTCTTGGGAAATAATAGTGACGTTTCTGCAACACTTCCAGGAGAAGTCGTGAAAGATGTTGGA
TTTTATGATTACAACTCAAAATATATTGATAATAAAATCCAAATGGACATTCCTGCAAAAGTTTCAGCAGATTTAGCTCA
AAAAATACAAGAATATGCCAAGAAAGCTTATAAAGCAGTTAATGGAGCTGGTTTGTCACGTTGTGATTTCTTTGTGACTA
AAGATGGAAATGTGTATCTTAATGAAGTCAATGCCATCCCTGGTTTCACTCAATGGTCAATGTATCCATTGCTTTGGGAA
AATATGGGCTTGTCTTACTCTGATTTAATTGAAAAATTAGTTGATTTAGCTAAAGAAACTTTTGAAACACGGGAAAATCA
TCTCTTATAA

Upstream 100 bases:

>100_bases
AAAAAAGACAATTATTGACGTTTTGTATTATAAAGCAATCTTAAAAATGGTCAGTAAAAAAGAAGCGACTGACAAGGCAG
TCAGTAAAAAAGGAAGAAAT

Downstream 100 bases:

>100_bases
AAAATACGAATCTGTCGGTAAATTAATTGCTGACAGATTTTTTCATCAGTAAAAAAGGCAAAAAGTACCACTGCTTATGG
GCTGTGTTACTTTTTTCTAT

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MSKETLILLYGGRSAEREVSVLSAESVMRAVNYSRFIVKTYFITKGGEFIKTQEFTDRPAEEEKLLTNDLAESYPKISPA
AIYEKDAVVFPVLHGPMGEDGSIQGFLEILRLAYVGPNILSASSTMDKLLAKHVFEAVGVPQVPYVAAFADENQVEIAQE
VVEKLDFPVFVKPANMGSSVGISKVDDLADLQPALSEAYKYDNRVVIEQGVDAREIECAVLGNNSDVSATLPGEVVKDVG
FYDYNSKYIDNKIQMDIPAKVSADLAQKIQEYAKKAYKAVNGAGLSRCDFFVTKDGNVYLNEVNAIPGFTQWSMYPLLWE
NMGLSYSDLIEKLVDLAKETFETRENHLL

Sequences:

>Translated_349_residues
MSKETLILLYGGRSAEREVSVLSAESVMRAVNYSRFIVKTYFITKGGEFIKTQEFTDRPAEEEKLLTNDLAESYPKISPA
AIYEKDAVVFPVLHGPMGEDGSIQGFLEILRLAYVGPNILSASSTMDKLLAKHVFEAVGVPQVPYVAAFADENQVEIAQE
VVEKLDFPVFVKPANMGSSVGISKVDDLADLQPALSEAYKYDNRVVIEQGVDAREIECAVLGNNSDVSATLPGEVVKDVG
FYDYNSKYIDNKIQMDIPAKVSADLAQKIQEYAKKAYKAVNGAGLSRCDFFVTKDGNVYLNEVNAIPGFTQWSMYPLLWE
NMGLSYSDLIEKLVDLAKETFETRENHLL
>Mature_348_residues
SKETLILLYGGRSAEREVSVLSAESVMRAVNYSRFIVKTYFITKGGEFIKTQEFTDRPAEEEKLLTNDLAESYPKISPAA
IYEKDAVVFPVLHGPMGEDGSIQGFLEILRLAYVGPNILSASSTMDKLLAKHVFEAVGVPQVPYVAAFADENQVEIAQEV
VEKLDFPVFVKPANMGSSVGISKVDDLADLQPALSEAYKYDNRVVIEQGVDAREIECAVLGNNSDVSATLPGEVVKDVGF
YDYNSKYIDNKIQMDIPAKVSADLAQKIQEYAKKAYKAVNGAGLSRCDFFVTKDGNVYLNEVNAIPGFTQWSMYPLLWEN
MGLSYSDLIEKLVDLAKETFETRENHLL

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=363, Percent_Identity=37.1900826446281, Blast_Score=244, Evalue=5e-66,
Organism=Escherichia coli, GI1786280, Length=343, Percent_Identity=31.7784256559767, Blast_Score=162, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 38678; Mature: 38547

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKETLILLYGGRSAEREVSVLSAESVMRAVNYSRFIVKTYFITKGGEFIKTQEFTDRPA
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCEEECCCCCCCCC
EEEKLLTNDLAESYPKISPAAIYEKDAVVFPVLHGPMGEDGSIQGFLEILRLAYVGPNIL
HHHHHHHHHHHHHCCCCCCHHEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHH
SASSTMDKLLAKHVFEAVGVPQVPYVAAFADENQVEIAQEVVEKLDFPVFVKPANMGSSV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
GISKVDDLADLQPALSEAYKYDNRVVIEQGVDAREIECAVLGNNSDVSATLPGEVVKDVG
CCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCCEEECCHHHHHHCC
FYDYNSKYIDNKIQMDIPAKVSADLAQKIQEYAKKAYKAVNGAGLSRCDFFVTKDGNVYL
CCCCCCCEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEE
NEVNAIPGFTQWSMYPLLWENMGLSYSDLIEKLVDLAKETFETRENHLL
EEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKETLILLYGGRSAEREVSVLSAESVMRAVNYSRFIVKTYFITKGGEFIKTQEFTDRPA
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCEEECCCCCCCCC
EEEKLLTNDLAESYPKISPAAIYEKDAVVFPVLHGPMGEDGSIQGFLEILRLAYVGPNIL
HHHHHHHHHHHHHCCCCCCHHEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHH
SASSTMDKLLAKHVFEAVGVPQVPYVAAFADENQVEIAQEVVEKLDFPVFVKPANMGSSV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
GISKVDDLADLQPALSEAYKYDNRVVIEQGVDAREIECAVLGNNSDVSATLPGEVVKDVG
CCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCCEEECCHHHHHHCC
FYDYNSKYIDNKIQMDIPAKVSADLAQKIQEYAKKAYKAVNGAGLSRCDFFVTKDGNVYL
CCCCCCCEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEE
NEVNAIPGFTQWSMYPLLWENMGLSYSDLIEKLVDLAKETFETRENHLL
EEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]