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The map label for this gene is rmlD [H]

Identifier: 125623087

GI number: 125623087

Start: 198457

End: 199356

Strand: Direct

Name: rmlD [H]

Synonym: llmg_0210

Alternate gene names: 125623087

Gene position: 198457-199356 (Clockwise)

Preceding gene: 125623086

Following gene: 125623088

Centisome position: 7.85

GC content: 39.33

Gene sequence:

>900_bases
ATGATTTTAATAACAGGTGGAAATGGTCAGCTTGGAACAGAGTTGCGTCATTTACTTGACGAACGTGGCATGGCTTATAC
AGCAACAGATGCCAAAGAACTTGATATCACTGATAAAGTTGCGGTTGATACATTCTTTGACAAAAACAAACCTGAACTTG
TTTATCACTGCGCAGCTTATACAGCTGTTGATAAAGCCGAAGATGAAGGTCGCGAGCTTGATGAAAAAATCAATGTCGAT
GGCACAAAAAATGTGGCCGAAGCAGCTGCACGCGTAGGTGCAACATTGGTTTATATTTCAACAGACTATGTTTTTGGTGG
GACTTTGCCAATTGGTCAAGAATGGCCAGTTGATGCACCAAAAGCGCCTGAATCTGAATATGGACGGACAAAACACCTTG
GTGAAGAAGCTGTTGTTGCCTCTGGTGTAAAACATTATATCGTTCGTACTGCTTGGGTTTTTGGTTCATATGGGCCAAAC
TTTGTCTTTACAATGCAAAATTTGGCGACTCGTTTTCCTGAATTGACCGTTGTTAATGACCAACATGGTCGTCCAACATG
GACACGTACGCTTGCTGAATTTATGGTTTATTTAGTTAATGAAAAAGCTGACTTTGGTTTTTATCATTTAACAAATGATG
CTGCACCTGGCGAAGACGTAACTTGGTTTGATTTTGCCAAAGAAATTTTGAAAGATACAGATGTGCTTGTTAAGCCTGTA
GATTCTAGTAAATTCCCAGCAAAAGCCAAACGTCCATTTAATTCAACAATGAGTCTTGAAAAAGCAAAAGCGACAGGTTT
TGTCATTCCTACATGGCAAGATGCTTTAGCAAGTATGCTTGAAAAAGGCGATTTACGTGAAAATAAAGATGGCCTAAAAG
GCGAGATTAGTAAAAAATAA

Upstream 100 bases:

>100_bases
AGGAAAAAGAGTCACATAGTCCAAAGGTTGCTGACAGGTTTGTCAGTAGATTTGAATAATGAGTTTCTTTCTATCAGTAA
AAATAAGAATTGGAGAATGT

Downstream 100 bases:

>100_bases
TGACTTTGTCATTAAATATAAAGTAATTGAAAATTCTGTCAGCGACCTGCATAAGCTCGTCAGTACTGACAGAATTCTCA
TTTTGAGAGGCTAAATAAAA

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MILITGGNGQLGTELRHLLDERGMAYTATDAKELDITDKVAVDTFFDKNKPELVYHCAAYTAVDKAEDEGRELDEKINVD
GTKNVAEAAARVGATLVYISTDYVFGGTLPIGQEWPVDAPKAPESEYGRTKHLGEEAVVASGVKHYIVRTAWVFGSYGPN
FVFTMQNLATRFPELTVVNDQHGRPTWTRTLAEFMVYLVNEKADFGFYHLTNDAAPGEDVTWFDFAKEILKDTDVLVKPV
DSSKFPAKAKRPFNSTMSLEKAKATGFVIPTWQDALASMLEKGDLRENKDGLKGEISKK

Sequences:

>Translated_299_residues
MILITGGNGQLGTELRHLLDERGMAYTATDAKELDITDKVAVDTFFDKNKPELVYHCAAYTAVDKAEDEGRELDEKINVD
GTKNVAEAAARVGATLVYISTDYVFGGTLPIGQEWPVDAPKAPESEYGRTKHLGEEAVVASGVKHYIVRTAWVFGSYGPN
FVFTMQNLATRFPELTVVNDQHGRPTWTRTLAEFMVYLVNEKADFGFYHLTNDAAPGEDVTWFDFAKEILKDTDVLVKPV
DSSKFPAKAKRPFNSTMSLEKAKATGFVIPTWQDALASMLEKGDLRENKDGLKGEISKK
>Mature_299_residues
MILITGGNGQLGTELRHLLDERGMAYTATDAKELDITDKVAVDTFFDKNKPELVYHCAAYTAVDKAEDEGRELDEKINVD
GTKNVAEAAARVGATLVYISTDYVFGGTLPIGQEWPVDAPKAPESEYGRTKHLGEEAVVASGVKHYIVRTAWVFGSYGPN
FVFTMQNLATRFPELTVVNDQHGRPTWTRTLAEFMVYLVNEKADFGFYHLTNDAAPGEDVTWFDFAKEILKDTDVLVKPV
DSSKFPAKAKRPFNSTMSLEKAKATGFVIPTWQDALASMLEKGDLRENKDGLKGEISKK

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=161, Percent_Identity=33.5403726708075, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI11034825, Length=161, Percent_Identity=33.5403726708075, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1788352, Length=299, Percent_Identity=33.7792642140468, Blast_Score=140, Evalue=9e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 33127; Mature: 33127

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILITGGNGQLGTELRHLLDERGMAYTATDAKELDITDKVAVDTFFDKNKPELVYHCAAY
CEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCEEEEEHHH
TAVDKAEDEGRELDEKINVDGTKNVAEAAARVGATLVYISTDYVFGGTLPIGQEWPVDAP
HHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHCEEEEEEEECEEECCCCCCCCCCCCCCC
KAPESEYGRTKHLGEEAVVASGVKHYIVRTAWVFGSYGPNFVFTMQNLATRFPELTVVND
CCCCHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHCCCEEEEEC
QHGRPTWTRTLAEFMVYLVNEKADFGFYHLTNDAAPGEDVTWFDFAKEILKDTDVLVKPV
CCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECC
DSSKFPAKAKRPFNSTMSLEKAKATGFVIPTWQDALASMLEKGDLRENKDGLKGEISKK
CCCCCCCHHCCCCCCHHHHHHHHHCCEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MILITGGNGQLGTELRHLLDERGMAYTATDAKELDITDKVAVDTFFDKNKPELVYHCAAY
CEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCEEEEEHHH
TAVDKAEDEGRELDEKINVDGTKNVAEAAARVGATLVYISTDYVFGGTLPIGQEWPVDAP
HHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHCEEEEEEEECEEECCCCCCCCCCCCCCC
KAPESEYGRTKHLGEEAVVASGVKHYIVRTAWVFGSYGPNFVFTMQNLATRFPELTVVND
CCCCHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHCCCEEEEEC
QHGRPTWTRTLAEFMVYLVNEKADFGFYHLTNDAAPGEDVTWFDFAKEILKDTDVLVKPV
CCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECC
DSSKFPAKAKRPFNSTMSLEKAKATGFVIPTWQDALASMLEKGDLRENKDGLKGEISKK
CCCCCCCHHCCCCCCHHHHHHHHHCCEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]