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The map label for this gene is gatA [H]

Identifier: 125623053

GI number: 125623053

Start: 164828

End: 166288

Strand: Direct

Name: gatA [H]

Synonym: llmg_0175

Alternate gene names: 125623053

Gene position: 164828-166288 (Clockwise)

Preceding gene: 125623052

Following gene: 125623054

Centisome position: 6.52

GC content: 38.6

Gene sequence:

>1461_bases
ATGTCATACAATAATAAATCAATCAAAGAACTACATGAGCTTCTTGTAAATAAAGAAATTTCGGCTCTTGAATTGACAAA
GGCGACGCTTTCTGACATTCAAGCACGTGAACCACAAATTGACGCTTTTTTGAAAGTCACAGAAGAAAAAGCTTTGAAAG
AAGCTGCAGCAATTGATGCTCGTGGAATTAATCCAGACGTTGTGACTGATGGAATTTCGATTGGTGTCAAAGATAATATC
GTTACAGAAGGTATTGAAACAACAGCAGCTTCTAAGATTCTTGGGGGTTGGATTCCTCCTTATAATGCAACAGTTGCTAA
TAAATTGAGCCAATCTGGGTTAATTACAATTGGTAAACTCAACATGGATGAATTCGCCATGGGTGGGTCTGGTGAAAATT
CTTCGATTAAGCCAACTAAAAACGCCTGGGACCAAACAAAAGTACCTGGTGGCTCTTCATCAGGTTCCGCAGCTTCAGTT
GCTTCTGGTGAGGTTCGTTTATCACTCGGTTCAGATACTGGTGGCTCAATCCGTCAGCCAGCTGCTTTCAACGGAATTGT
TGGTTTAAAACCAACTTATGGTCGTGTCAGCCGTTTTGGCTTGATTGCTTTTGCTTCATCATTAGACCAAATCGGTCCAT
TGACACCAACAGTTGAAGAAAATGCTCAACTTTTAAATGTCATTTCTGGCTTTGATAAAAATGATAGTACGTCATCAAAT
GTTGCGGTTCCTGACTTTACAAGTAAAATTGGTCAAGACATTAAAGGAATGAAAATTGCTTTACCTAAAGAATATTTTGG
TGAAGGAATTGATGAAAAAGTCAAAGAACAAATTTTGGCAGCAGCTAAACATTTGGAAAAACTAGGTGCCATCGTTGAAG
AAGTCAGTCTTCCTCATAGTAAATATGGTGTCGCAGTTTATTATATTATCGCCTCTTCTGAAGCTAGCTCGAACCTTCAA
CGTTTCGATGGAATTCGTTATGGTTATCGCGCACAAGACATCAAAAACCTTGAAGATTTGTATGTGAAATCACGTTCTGA
AGGATTCGGTCCTGAGGTTCAACGTCGAATTATGTTGGGAACTTTCAGTCTGTCAGCCGGTTCTTATGATAAACATTTCA
AAAAAGCAGGACAAGTTCGTACTTTAATCATCAATGATTTTGCTAAAGTTTTTGAAAAATATGATTTGATTTTAGGACCA
ACAACTCCAACACCAGCTTGGGATTTAGGAGCACGTGTTGATGACCCAATCTCAATGTATTTGGCAGATTTATTGACTAT
TCCAGTCAACTTAGCTGGTCTTCCAGGAATTTCGATTCCTGCTGGTTTTGCCGATGGACTACCTGTGGGAATGCAATTGA
TTGGTAAACGTTACGATGAAGAAACGATTTATCAAGTAGCAGCTGCTTTTGAAGCAACGACTGATTTCCACAAAAAACAA
CCGATTATTTTTGGAAAATAA

Upstream 100 bases:

>100_bases
AAAAAGGACTTGATCGTGAAAAACTCATGGCAGCTGTTCCTGAAAAAGAAGACGGCTTTATCAAAGTTCCAGCAATGCTT
TCTGATGGAGGAGATGCCTA

Downstream 100 bases:

>100_bases
AAATGGAAAGTATCGGAGAAAATAATGAACTTTGAAACAGTAATTGGGCTTGAAGTCCACGTTGAGTTAAGTACAAACTC
AAAAATTTTCAGTCCCGCCT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MSYNNKSIKELHELLVNKEISALELTKATLSDIQAREPQIDAFLKVTEEKALKEAAAIDARGINPDVVTDGISIGVKDNI
VTEGIETTAASKILGGWIPPYNATVANKLSQSGLITIGKLNMDEFAMGGSGENSSIKPTKNAWDQTKVPGGSSSGSAASV
ASGEVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGRVSRFGLIAFASSLDQIGPLTPTVEENAQLLNVISGFDKNDSTSSN
VAVPDFTSKIGQDIKGMKIALPKEYFGEGIDEKVKEQILAAAKHLEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQ
RFDGIRYGYRAQDIKNLEDLYVKSRSEGFGPEVQRRIMLGTFSLSAGSYDKHFKKAGQVRTLIINDFAKVFEKYDLILGP
TTPTPAWDLGARVDDPISMYLADLLTIPVNLAGLPGISIPAGFADGLPVGMQLIGKRYDEETIYQVAAAFEATTDFHKKQ
PIIFGK

Sequences:

>Translated_486_residues
MSYNNKSIKELHELLVNKEISALELTKATLSDIQAREPQIDAFLKVTEEKALKEAAAIDARGINPDVVTDGISIGVKDNI
VTEGIETTAASKILGGWIPPYNATVANKLSQSGLITIGKLNMDEFAMGGSGENSSIKPTKNAWDQTKVPGGSSSGSAASV
ASGEVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGRVSRFGLIAFASSLDQIGPLTPTVEENAQLLNVISGFDKNDSTSSN
VAVPDFTSKIGQDIKGMKIALPKEYFGEGIDEKVKEQILAAAKHLEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQ
RFDGIRYGYRAQDIKNLEDLYVKSRSEGFGPEVQRRIMLGTFSLSAGSYDKHFKKAGQVRTLIINDFAKVFEKYDLILGP
TTPTPAWDLGARVDDPISMYLADLLTIPVNLAGLPGISIPAGFADGLPVGMQLIGKRYDEETIYQVAAAFEATTDFHKKQ
PIIFGK
>Mature_485_residues
SYNNKSIKELHELLVNKEISALELTKATLSDIQAREPQIDAFLKVTEEKALKEAAAIDARGINPDVVTDGISIGVKDNIV
TEGIETTAASKILGGWIPPYNATVANKLSQSGLITIGKLNMDEFAMGGSGENSSIKPTKNAWDQTKVPGGSSSGSAASVA
SGEVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGRVSRFGLIAFASSLDQIGPLTPTVEENAQLLNVISGFDKNDSTSSNV
AVPDFTSKIGQDIKGMKIALPKEYFGEGIDEKVKEQILAAAKHLEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQR
FDGIRYGYRAQDIKNLEDLYVKSRSEGFGPEVQRRIMLGTFSLSAGSYDKHFKKAGQVRTLIINDFAKVFEKYDLILGPT
TPTPAWDLGARVDDPISMYLADLLTIPVNLAGLPGISIPAGFADGLPVGMQLIGKRYDEETIYQVAAAFEATTDFHKKQP
IIFGK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=498, Percent_Identity=38.1526104417671, Blast_Score=311, Evalue=1e-84,
Organism=Homo sapiens, GI195972892, Length=484, Percent_Identity=23.5537190082645, Blast_Score=110, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17543272, Length=435, Percent_Identity=37.4712643678161, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17537465, Length=495, Percent_Identity=23.2323232323232, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17556264, Length=224, Percent_Identity=29.0178571428571, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71990152, Length=208, Percent_Identity=29.8076923076923, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17538252, Length=254, Percent_Identity=24.0157480314961, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=415, Percent_Identity=35.9036144578313, Blast_Score=240, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6319685, Length=453, Percent_Identity=29.8013245033113, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24648113, Length=501, Percent_Identity=39.5209580838323, Blast_Score=322, Evalue=3e-88,
Organism=Drosophila melanogaster, GI24652985, Length=484, Percent_Identity=25.4132231404959, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19922090, Length=484, Percent_Identity=25.4132231404959, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24652981, Length=484, Percent_Identity=25.4132231404959, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24652983, Length=484, Percent_Identity=25.4132231404959, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI45550774, Length=192, Percent_Identity=34.8958333333333, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24648435, Length=197, Percent_Identity=35.0253807106599, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24648437, Length=197, Percent_Identity=35.0253807106599, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24644968, Length=511, Percent_Identity=23.4833659491194, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI161078093, Length=498, Percent_Identity=23.6947791164659, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648441, Length=127, Percent_Identity=39.3700787401575, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648439, Length=127, Percent_Identity=39.3700787401575, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356731, Length=340, Percent_Identity=24.7058823529412, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52143; Mature: 52012

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYNNKSIKELHELLVNKEISALELTKATLSDIQAREPQIDAFLKVTEEKALKEAAAIDA
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
RGINPDVVTDGISIGVKDNIVTEGIETTAASKILGGWIPPYNATVANKLSQSGLITIGKL
CCCCCCCEECCEEECCCCCHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCEEEEEC
NMDEFAMGGSGENSSIKPTKNAWDQTKVPGGSSSGSAASVASGEVRLSLGSDTGGSIRQP
CHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCEEEEEECCCCCCCCCCC
AAFNGIVGLKPTYGRVSRFGLIAFASSLDQIGPLTPTVEENAQLLNVISGFDKNDSTSSN
CHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
VAVPDFTSKIGQDIKGMKIALPKEYFGEGIDEKVKEQILAAAKHLEKLGAIVEEVSLPHS
EECCCHHHHHCCCCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KYGVAVYYIIASSEASSNLQRFDGIRYGYRAQDIKNLEDLYVKSRSEGFGPEVQRRIMLG
CCCEEEEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHEEEEE
TFSLSAGSYDKHFKKAGQVRTLIINDFAKVFEKYDLILGPTTPTPAWDLGARVDDPISMY
EEECCCCCHHHHHHHHCCEEEHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCHHHHH
LADLLTIPVNLAGLPGISIPAGFADGLPVGMQLIGKRYDEETIYQVAAAFEATTDFHKKQ
HHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
PIIFGK
CCCCCC
>Mature Secondary Structure 
SYNNKSIKELHELLVNKEISALELTKATLSDIQAREPQIDAFLKVTEEKALKEAAAIDA
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
RGINPDVVTDGISIGVKDNIVTEGIETTAASKILGGWIPPYNATVANKLSQSGLITIGKL
CCCCCCCEECCEEECCCCCHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCEEEEEC
NMDEFAMGGSGENSSIKPTKNAWDQTKVPGGSSSGSAASVASGEVRLSLGSDTGGSIRQP
CHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCEEEEEECCCCCCCCCCC
AAFNGIVGLKPTYGRVSRFGLIAFASSLDQIGPLTPTVEENAQLLNVISGFDKNDSTSSN
CHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
VAVPDFTSKIGQDIKGMKIALPKEYFGEGIDEKVKEQILAAAKHLEKLGAIVEEVSLPHS
EECCCHHHHHCCCCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KYGVAVYYIIASSEASSNLQRFDGIRYGYRAQDIKNLEDLYVKSRSEGFGPEVQRRIMLG
CCCEEEEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHEEEEE
TFSLSAGSYDKHFKKAGQVRTLIINDFAKVFEKYDLILGPTTPTPAWDLGARVDDPISMY
EEECCCCCHHHHHHHHCCEEEHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCHHHHH
LADLLTIPVNLAGLPGISIPAGFADGLPVGMQLIGKRYDEETIYQVAAAFEATTDFHKKQ
HHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
PIIFGK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]