Click here to switch to the map view.

The map label for this gene is gatB [H]

Identifier: 125623054

GI number: 125623054

Start: 166313

End: 167746

Strand: Direct

Name: gatB [H]

Synonym: llmg_0176

Alternate gene names: 125623054

Gene position: 166313-167746 (Clockwise)

Preceding gene: 125623053

Following gene: 125623055

Centisome position: 6.57

GC content: 39.19

Gene sequence:

>1434_bases
ATGAACTTTGAAACAGTAATTGGGCTTGAAGTCCACGTTGAGTTAAGTACAAACTCAAAAATTTTCAGTCCCGCCTCTAC
AAAATTTGGTGGCGACCCTAATAGCAATACAAATGTGATTGACTGGTCACTTCCTGGTGTTTTACCAGTCATGAATAAAG
GGGTGATTGATAGCGGGATTAAAGCGGCTCTTGCTTTGAATATGGATATTCACAAATCAATGCACTTTGACCGCAAAAAT
TATTTCTATCCTGATAATCCCAAAGCTTACCAAATTTCACAATTTGATGAGCCAATTGGCTACAATGGTTCAATCGAGAT
TGAACTTGAAGATGGTCATAAAGCAACGATTCGTATTGAGCGTGCTCACTTGGAAGAAGATGCTGGGAAAAATACTCACG
GAACAGATGGTTACTCTTATGTTGACTTGAATCGTCAAGGTGTGCCATTGATTGAAATTGTTTCAGAAGCTGATATGCGC
ACTCCAGAAGAAGCTTATGCCTATTTGACAGCGCTCAAAGAAGCTATTCTTTACACAGGAATTTCTGATGTGAAGATGGA
AGAAGGTTCAATGCGTTGTGACGCCAATGTTTCGCTTCGTCCTTATGGGCAAGAAGCTTTCGGTGTGAAGACAGAAGTTA
AAAATATGAACTCTTTTAGCAATGTCAAAAAAGCTTTGGACTTTGAAGTGGCACGTCAAGCTAAAATTTTGCGTGCGGGT
GGCGAAATTCGTCAAGAAACACGTCGTTTTAATGACAAAACCGGTGAAACAATTTTGATGCGTGTCAAAGAAGGTGCGTC
TGATTATCGTTACTTCCCAGAGCCAGATGTTCCTCGTTTTGAGATTTCTGACGAATGGATTGAACAAATGCGCGCGAGTC
TTCCAATGACAGCAACTGCAAGACGTGCACATTATATTAATGATTTGGGCTTATCTGACTATGATGCGCGTCAATTGACA
GCAACTAAAGAAGTTTCTGATTTCTTTGATCAAGCGATTAAATTTGATACTGACCCTAAATTGGTTTCAAACTGGTTGCA
AGGCGAGGTCGCTCAATATTTGAATAGCGAGAAAAAAGAACTTCATGAGATTGGATTGACCCCAGAAAATCTGACAGAGA
TGATTCGTTTGATTTCAGATGGAACAATTTCTTCAAAAATTGCTAAAAAAGTCTTTATTGAATTAGCAAAAAATGGTGGA
TCTGCAGAAGAATTTGTCAAGAAGGCTGGTTTGGTCCAAATTTCTGATCCTGACCTTCTTTTGCCAATCATTCATGAGGT
CTTTGCTAAGAATGAACAATCTGTGGCCGATTATCGCGGTGGGAAACAAAATGCAGCCAAAGCTTTGGTTGGTCAATTGA
TGAAAGCAACTAAAGGGCAAGCTAATCCAACAGTCGCTCAAAAACTTTTGTATCAAGAATTAGATAATTTTTAA

Upstream 100 bases:

>100_bases
GATTTATCAAGTAGCAGCTGCTTTTGAAGCAACGACTGATTTCCACAAAAAACAACCGATTATTTTTGGAAAATAAAAAT
GGAAAGTATCGGAGAAAATA

Downstream 100 bases:

>100_bases
TAAATATAAATATCCGTCCTAATTTGGAACGGATATTTTTTATTATGCTTGATAAGTATGGTAAAATGTTGGCAGAATGA
GTTTGTCAGCAAAGATATAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MNFETVIGLEVHVELSTNSKIFSPASTKFGGDPNSNTNVIDWSLPGVLPVMNKGVIDSGIKAALALNMDIHKSMHFDRKN
YFYPDNPKAYQISQFDEPIGYNGSIEIELEDGHKATIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
TPEEAYAYLTALKEAILYTGISDVKMEEGSMRCDANVSLRPYGQEAFGVKTEVKNMNSFSNVKKALDFEVARQAKILRAG
GEIRQETRRFNDKTGETILMRVKEGASDYRYFPEPDVPRFEISDEWIEQMRASLPMTATARRAHYINDLGLSDYDARQLT
ATKEVSDFFDQAIKFDTDPKLVSNWLQGEVAQYLNSEKKELHEIGLTPENLTEMIRLISDGTISSKIAKKVFIELAKNGG
SAEEFVKKAGLVQISDPDLLLPIIHEVFAKNEQSVADYRGGKQNAAKALVGQLMKATKGQANPTVAQKLLYQELDNF

Sequences:

>Translated_477_residues
MNFETVIGLEVHVELSTNSKIFSPASTKFGGDPNSNTNVIDWSLPGVLPVMNKGVIDSGIKAALALNMDIHKSMHFDRKN
YFYPDNPKAYQISQFDEPIGYNGSIEIELEDGHKATIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
TPEEAYAYLTALKEAILYTGISDVKMEEGSMRCDANVSLRPYGQEAFGVKTEVKNMNSFSNVKKALDFEVARQAKILRAG
GEIRQETRRFNDKTGETILMRVKEGASDYRYFPEPDVPRFEISDEWIEQMRASLPMTATARRAHYINDLGLSDYDARQLT
ATKEVSDFFDQAIKFDTDPKLVSNWLQGEVAQYLNSEKKELHEIGLTPENLTEMIRLISDGTISSKIAKKVFIELAKNGG
SAEEFVKKAGLVQISDPDLLLPIIHEVFAKNEQSVADYRGGKQNAAKALVGQLMKATKGQANPTVAQKLLYQELDNF
>Mature_477_residues
MNFETVIGLEVHVELSTNSKIFSPASTKFGGDPNSNTNVIDWSLPGVLPVMNKGVIDSGIKAALALNMDIHKSMHFDRKN
YFYPDNPKAYQISQFDEPIGYNGSIEIELEDGHKATIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
TPEEAYAYLTALKEAILYTGISDVKMEEGSMRCDANVSLRPYGQEAFGVKTEVKNMNSFSNVKKALDFEVARQAKILRAG
GEIRQETRRFNDKTGETILMRVKEGASDYRYFPEPDVPRFEISDEWIEQMRASLPMTATARRAHYINDLGLSDYDARQLT
ATKEVSDFFDQAIKFDTDPKLVSNWLQGEVAQYLNSEKKELHEIGLTPENLTEMIRLISDGTISSKIAKKVFIELAKNGG
SAEEFVKKAGLVQISDPDLLLPIIHEVFAKNEQSVADYRGGKQNAAKALVGQLMKATKGQANPTVAQKLLYQELDNF

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=36.7676767676768, Blast_Score=297, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI115534094, Length=343, Percent_Identity=34.6938775510204, Blast_Score=213, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6319391, Length=502, Percent_Identity=28.4860557768924, Blast_Score=183, Evalue=4e-47,
Organism=Drosophila melanogaster, GI45550808, Length=496, Percent_Identity=30.8467741935484, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53341; Mature: 53341

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFETVIGLEVHVELSTNSKIFSPASTKFGGDPNSNTNVIDWSLPGVLPVMNKGVIDSGI
CCCCEEEEEEEEEEECCCCEEECCCCCCCCCCCCCCCCEEEEECCCHHHHHCCCCHHCCC
KAALALNMDIHKSMHFDRKNYFYPDNPKAYQISQFDEPIGYNGSIEIELEDGHKATIRIE
CEEEEECCHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCEEEEEEE
RAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMRTPEEAYAYLTALKEAILYTG
HHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
ISDVKMEEGSMRCDANVSLRPYGQEAFGVKTEVKNMNSFSNVKKALDFEVARQAKILRAG
CCCCEECCCCCEECCCCEECCCCCHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
GEIRQETRRFNDKTGETILMRVKEGASDYRYFPEPDVPRFEISDEWIEQMRASLPMTATA
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCCCHHHH
RRAHYINDLGLSDYDARQLTATKEVSDFFDQAIKFDTDPKLVSNWLQGEVAQYLNSEKKE
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHH
LHEIGLTPENLTEMIRLISDGTISSKIAKKVFIELAKNGGSAEEFVKKAGLVQISDPDLL
HHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCEEEECCCCHH
LPIIHEVFAKNEQSVADYRGGKQNAAKALVGQLMKATKGQANPTVAQKLLYQELDNF
HHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFETVIGLEVHVELSTNSKIFSPASTKFGGDPNSNTNVIDWSLPGVLPVMNKGVIDSGI
CCCCEEEEEEEEEEECCCCEEECCCCCCCCCCCCCCCCEEEEECCCHHHHHCCCCHHCCC
KAALALNMDIHKSMHFDRKNYFYPDNPKAYQISQFDEPIGYNGSIEIELEDGHKATIRIE
CEEEEECCHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCEEEEEEE
RAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMRTPEEAYAYLTALKEAILYTG
HHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
ISDVKMEEGSMRCDANVSLRPYGQEAFGVKTEVKNMNSFSNVKKALDFEVARQAKILRAG
CCCCEECCCCCEECCCCEECCCCCHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
GEIRQETRRFNDKTGETILMRVKEGASDYRYFPEPDVPRFEISDEWIEQMRASLPMTATA
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCCCHHHH
RRAHYINDLGLSDYDARQLTATKEVSDFFDQAIKFDTDPKLVSNWLQGEVAQYLNSEKKE
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHH
LHEIGLTPENLTEMIRLISDGTISSKIAKKVFIELAKNGGSAEEFVKKAGLVQISDPDLL
HHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCEEEECCCCHH
LPIIHEVFAKNEQSVADYRGGKQNAAKALVGQLMKATKGQANPTVAQKLLYQELDNF
HHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]