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The map label for this gene is rsmE [H]

Identifier: 125622990

GI number: 125622990

Start: 103858

End: 104610

Strand: Direct

Name: rsmE [H]

Synonym: llmg_0111

Alternate gene names: 125622990

Gene position: 103858-104610 (Clockwise)

Preceding gene: 125622989

Following gene: 125622991

Centisome position: 4.11

GC content: 38.38

Gene sequence:

>753_bases
ATGGCTAACCAATATTTTGTATTTAAAGAATTACCAAAAATCGGTGAGAAATTTACCATTGAAAACCAAGCCGCAGCTCA
CCATATTTTTACGGTCATGCGGGCACAGGCGGGTGATCAGTTACGCTTAGTTTTTGAAGAGGGGACAATTGGCCTGTCAG
AAATTATTTCGCCAGCTGACCAGTCGGTCAGATTGATTGAATTACTGACAGATTCATCAGAATTACCTGTCCAAGTCACA
GTTGCAGTTGGTTTTCCTAAAGGTGATAAATTAGATTTTATTGTTGAAAAATCAACTGAATTAGGCGCCACAGCCATTTG
GTCAGCACCTTTTAAAGCTTCTGTCAGTAAATGGGACCACAAAAAACTGACAAAAAAACAAGAAAAATTGGAAAAAGTTG
CTCTAGGAGCCGCCGAGCAATCTCGCAGACAATTGATTCCTAAAATTCAATTATTTGACCAATTTTCTAGATTGACAGAG
CAATTTTCTGATTTTGACCAGGTTCTTGTAGCCTATGAAGAGGCGGCAAAAATGGGCGAAAAATCAATGTTTAAACAAGC
ACTGACCGAGCTGTCAGCAAATCAAAAAGTCCTGATTATTTTTGGGCCAGAAGGTGGAATTAGTCCTGATGAAATTGCAA
AATTTGAAAATCTAGGGGCAAAAATGATTGGCTTAGGACCACGAATATTGCGAGCAGAAACAGCTCCCTTATACGCTTTA
TCAAGTATCTCAGCTTATTTTGAATTATTATAA

Upstream 100 bases:

>100_bases
TAGGACCTTGGAAATGAGTGCTTTATCTTTTAGCAAATGATAAAATAAAGTAAACAATTCATTGAAGTGAATTTTATGAA
ATAGATGATTGGAAGAGAAA

Downstream 100 bases:

>100_bases
ATAAAGAAAAAATTTTTAGGAAATCGATTATGAAAAAACGTGTACAACGTAACAAAAAACGAATTCGCTGGGCTTCAGTC
CTCACAGTTTTCGTTCTTTT

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MANQYFVFKELPKIGEKFTIENQAAAHHIFTVMRAQAGDQLRLVFEEGTIGLSEIISPADQSVRLIELLTDSSELPVQVT
VAVGFPKGDKLDFIVEKSTELGATAIWSAPFKASVSKWDHKKLTKKQEKLEKVALGAAEQSRRQLIPKIQLFDQFSRLTE
QFSDFDQVLVAYEEAAKMGEKSMFKQALTELSANQKVLIIFGPEGGISPDEIAKFENLGAKMIGLGPRILRAETAPLYAL
SSISAYFELL

Sequences:

>Translated_250_residues
MANQYFVFKELPKIGEKFTIENQAAAHHIFTVMRAQAGDQLRLVFEEGTIGLSEIISPADQSVRLIELLTDSSELPVQVT
VAVGFPKGDKLDFIVEKSTELGATAIWSAPFKASVSKWDHKKLTKKQEKLEKVALGAAEQSRRQLIPKIQLFDQFSRLTE
QFSDFDQVLVAYEEAAKMGEKSMFKQALTELSANQKVLIIFGPEGGISPDEIAKFENLGAKMIGLGPRILRAETAPLYAL
SSISAYFELL
>Mature_249_residues
ANQYFVFKELPKIGEKFTIENQAAAHHIFTVMRAQAGDQLRLVFEEGTIGLSEIISPADQSVRLIELLTDSSELPVQVTV
AVGFPKGDKLDFIVEKSTELGATAIWSAPFKASVSKWDHKKLTKKQEKLEKVALGAAEQSRRQLIPKIQLFDQFSRLTEQ
FSDFDQVLVAYEEAAKMGEKSMFKQALTELSANQKVLIIFGPEGGISPDEIAKFENLGAKMIGLGPRILRAETAPLYALS
SISAYFELL

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=226, Percent_Identity=29.646017699115, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27740; Mature: 27609

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANQYFVFKELPKIGEKFTIENQAAAHHIFTVMRAQAGDQLRLVFEEGTIGLSEIISPAD
CCCCEEHHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHCCCCC
QSVRLIELLTDSSELPVQVTVAVGFPKGDKLDFIVEKSTELGATAIWSAPFKASVSKWDH
HHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCEEEECCCCHHHHHHHHH
KKLTKKQEKLEKVALGAAEQSRRQLIPKIQLFDQFSRLTEQFSDFDQVLVAYEEAAKMGE
HHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
KSMFKQALTELSANQKVLIIFGPEGGISPDEIAKFENLGAKMIGLGPRILRAETAPLYAL
HHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEECCCCHHHHCCCCCHHHH
SSISAYFELL
HHHHHHHHCC
>Mature Secondary Structure 
ANQYFVFKELPKIGEKFTIENQAAAHHIFTVMRAQAGDQLRLVFEEGTIGLSEIISPAD
CCCEEHHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHCCCCC
QSVRLIELLTDSSELPVQVTVAVGFPKGDKLDFIVEKSTELGATAIWSAPFKASVSKWDH
HHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCEEEECCCCHHHHHHHHH
KKLTKKQEKLEKVALGAAEQSRRQLIPKIQLFDQFSRLTEQFSDFDQVLVAYEEAAKMGE
HHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
KSMFKQALTELSANQKVLIIFGPEGGISPDEIAKFENLGAKMIGLGPRILRAETAPLYAL
HHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEECCCCHHHHCCCCCHHHH
SSISAYFELL
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]