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The map label for this gene is cysD [H]

Identifier: 125622970

GI number: 125622970

Start: 86633

End: 87913

Strand: Reverse

Name: cysD [H]

Synonym: llmg_0091

Alternate gene names: 125622970

Gene position: 87913-86633 (Counterclockwise)

Preceding gene: 125622971

Following gene: 125622969

Centisome position: 3.48

GC content: 40.12

Gene sequence:

>1281_bases
ATGACTGATCATAATTATAAATTTGATACATTGCAAGTTCATGCGGGACAAGAGCCTGATCCAGTTACAGGAGCACGCGC
TGTTCCACTTTACCAAACCACTTCTTTTGTCTTTAACAATTCAGATCATGCTGAGGCACGATTTGCTCTACAAGACCCAG
GGGCCATATACTCACGTTTAGGAAATCCAACCAACGATGTTTTTGAAGCACGCATCGCAGCTCTTGAAGGTGGGAGTGCA
GCCCTTGGTGTTGGTTCTGGCTCTGCGGCCATCACTTATGCCATCTTGAATATCGCTACAGTCGGTGATAATATCGTTTC
TGCAAGTACACTCTATGGTGGAACCTATCATCTTTTTTCTGGGACTTTACCAAAATACGGAATTACAACTAAATTTGTCA
ATCCAGATGACCCGAAGAATTTTGAAGAGGCGATTGATGAAAAGACCAAAGCTATTTATTATGAAACGCTAGGTAACCCT
GGAAATAACGTCATTGATTATGATGCTGTTGGTGCAATCGCTAAAAAACATGGCATTCCTGTCATTGTTGATGCCACATT
TACTACTCCTGTAACCTTTAAACCTTTTGAACACGGCGCTAATGTAATTGTTCACTCTGCAACAAAATTCATTGGTGGTC
ACGGAACTTCTATTGGCGGGGTAATTGTTGATGGTGGAAACTTTGATTGGGCCAATGGGAAATTTCCTGATTTTACACAA
GCCGATGAAATCTACAATGGCATTAAATTTGCCGAATTGGGTGAAATTGCTTTTGTAACAAGAATCCGAGCTATTTTACT
AAGAGATACGGGGGCAGCCCTTTCACCTTTTCATTCTTGGCTTTTCTTGCAAGGACTCGAAACACTTTCACTTCGTGTTG
AACGTCATATTGCCAATACTAAAAAGATTGTAGATTTCTTAGACAAACACCCGAAAGTTGAACTTGTCAATCACCCTCTG
CTTGAATCTAATGCTTACCATGCGCTTTATCAAAAATATTATCCGAAAGATGCTGGCTCTATCTTTACCTTTGAACTCAA
GGATAAAGATGAGAAAAAAGCGAGAGACCTGATTGACCATCTCGAAATTTTCTCCCTTTTGGCCAATGTTGGAGATACAA
AATCACTTGCTATTCATCCTGCTTCAACCACTCACCAACAACTCAATGATGAAGAACTTGCTAGTGCAGGAATTTCTAAA
GGAACAATTCGTTTGTCAGTCGGCATTGAAGATGTGACTGATTTGATAGCGGATTTAGAACAAGCATTGGAAAAAATATA
A

Upstream 100 bases:

>100_bases
AGAAAGCTATGGTACAAGAATTGCTGACGAATAATCAACGAATGTGATCAATAAGTATTTTAAATTCTAATCTTAAACTT
TTCAAATAGGAGAAAAAAAT

Downstream 100 bases:

>100_bases
AAAAATGAGCGGTAACCAGCCGCTCATCTTTTATTTAGGAGTAAACTTTTATGAAAAATTTTATTGGAATAAGTATATTA
TAGTGAAACTCACTTAAGTT

Product: O-acetylhomoserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTDHNYKFDTLQVHAGQEPDPVTGARAVPLYQTTSFVFNNSDHAEARFALQDPGAIYSRLGNPTNDVFEARIAALEGGSA
ALGVGSGSAAITYAILNIATVGDNIVSASTLYGGTYHLFSGTLPKYGITTKFVNPDDPKNFEEAIDEKTKAIYYETLGNP
GNNVIDYDAVGAIAKKHGIPVIVDATFTTPVTFKPFEHGANVIVHSATKFIGGHGTSIGGVIVDGGNFDWANGKFPDFTQ
ADEIYNGIKFAELGEIAFVTRIRAILLRDTGAALSPFHSWLFLQGLETLSLRVERHIANTKKIVDFLDKHPKVELVNHPL
LESNAYHALYQKYYPKDAGSIFTFELKDKDEKKARDLIDHLEIFSLLANVGDTKSLAIHPASTTHQQLNDEELASAGISK
GTIRLSVGIEDVTDLIADLEQALEKI

Sequences:

>Translated_426_residues
MTDHNYKFDTLQVHAGQEPDPVTGARAVPLYQTTSFVFNNSDHAEARFALQDPGAIYSRLGNPTNDVFEARIAALEGGSA
ALGVGSGSAAITYAILNIATVGDNIVSASTLYGGTYHLFSGTLPKYGITTKFVNPDDPKNFEEAIDEKTKAIYYETLGNP
GNNVIDYDAVGAIAKKHGIPVIVDATFTTPVTFKPFEHGANVIVHSATKFIGGHGTSIGGVIVDGGNFDWANGKFPDFTQ
ADEIYNGIKFAELGEIAFVTRIRAILLRDTGAALSPFHSWLFLQGLETLSLRVERHIANTKKIVDFLDKHPKVELVNHPL
LESNAYHALYQKYYPKDAGSIFTFELKDKDEKKARDLIDHLEIFSLLANVGDTKSLAIHPASTTHQQLNDEELASAGISK
GTIRLSVGIEDVTDLIADLEQALEKI
>Mature_425_residues
TDHNYKFDTLQVHAGQEPDPVTGARAVPLYQTTSFVFNNSDHAEARFALQDPGAIYSRLGNPTNDVFEARIAALEGGSAA
LGVGSGSAAITYAILNIATVGDNIVSASTLYGGTYHLFSGTLPKYGITTKFVNPDDPKNFEEAIDEKTKAIYYETLGNPG
NNVIDYDAVGAIAKKHGIPVIVDATFTTPVTFKPFEHGANVIVHSATKFIGGHGTSIGGVIVDGGNFDWANGKFPDFTQA
DEIYNGIKFAELGEIAFVTRIRAILLRDTGAALSPFHSWLFLQGLETLSLRVERHIANTKKIVDFLDKHPKVELVNHPLL
ESNAYHALYQKYYPKDAGSIFTFELKDKDEKKARDLIDHLEIFSLLANVGDTKSLAIHPASTTHQQLNDEELASAGISKG
TIRLSVGIEDVTDLIADLEQALEKI

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=424, Percent_Identity=33.0188679245283, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI299473759, Length=424, Percent_Identity=29.9528301886792, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI25453487, Length=423, Percent_Identity=29.3144208037825, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1790375, Length=414, Percent_Identity=31.6425120772947, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1789383, Length=430, Percent_Identity=26.2790697674419, Blast_Score=138, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI71980708, Length=413, Percent_Identity=32.9297820823245, Blast_Score=209, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17538055, Length=420, Percent_Identity=33.8095238095238, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17560056, Length=420, Percent_Identity=34.0476190476191, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=49.5391705069124, Blast_Score=360, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6319307, Length=426, Percent_Identity=31.6901408450704, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6321170, Length=337, Percent_Identity=27.299703264095, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6321254, Length=444, Percent_Identity=25.6756756756757, Blast_Score=112, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6321904, Length=429, Percent_Identity=23.7762237762238, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19922554, Length=422, Percent_Identity=32.9383886255924, Blast_Score=197, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46279; Mature: 46148

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDHNYKFDTLQVHAGQEPDPVTGARAVPLYQTTSFVFNNSDHAEARFALQDPGAIYSRL
CCCCCEEEEEEEEECCCCCCCCCCCEEEEEEEEEEEEECCCCCCCEEEEECCCHHHHHHC
GNPTNDVFEARIAALEGGSAALGVGSGSAAITYAILNIATVGDNIVSASTLYGGTYHLFS
CCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEHHHCCCCEEEEEC
GTLPKYGITTKFVNPDDPKNFEEAIDEKTKAIYYETLGNPGNNVIDYDAVGAIAKKHGIP
CCCCCCCCEEEECCCCCCCHHHHHHHHHHHEEEEEECCCCCCCEECHHHHHHHHHHCCCC
VIVDATFTTPVTFKPFEHGANVIVHSATKFIGGHGTSIGGVIVDGGNFDWANGKFPDFTQ
EEEEECCCCCEEECCHHCCCCEEEEEHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCC
ADEIYNGIKFAELGEIAFVTRIRAILLRDTGAALSPFHSWLFLQGLETLSLRVERHIANT
HHHHHCCCCHHHHCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKIVDFLDKHPKVELVNHPLLESNAYHALYQKYYPKDAGSIFTFELKDKDEKKARDLIDH
HHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHH
LEIFSLLANVGDTKSLAIHPASTTHQQLNDEELASAGISKGTIRLSVGIEDVTDLIADLE
HHHHHHHHCCCCCCEEEEECCCCHHHHCCHHHHHHCCCCCCEEEEEECHHHHHHHHHHHH
QALEKI
HHHHCC
>Mature Secondary Structure 
TDHNYKFDTLQVHAGQEPDPVTGARAVPLYQTTSFVFNNSDHAEARFALQDPGAIYSRL
CCCCEEEEEEEEECCCCCCCCCCCEEEEEEEEEEEEECCCCCCCEEEEECCCHHHHHHC
GNPTNDVFEARIAALEGGSAALGVGSGSAAITYAILNIATVGDNIVSASTLYGGTYHLFS
CCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEHHHCCCCEEEEEC
GTLPKYGITTKFVNPDDPKNFEEAIDEKTKAIYYETLGNPGNNVIDYDAVGAIAKKHGIP
CCCCCCCCEEEECCCCCCCHHHHHHHHHHHEEEEEECCCCCCCEECHHHHHHHHHHCCCC
VIVDATFTTPVTFKPFEHGANVIVHSATKFIGGHGTSIGGVIVDGGNFDWANGKFPDFTQ
EEEEECCCCCEEECCHHCCCCEEEEEHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCC
ADEIYNGIKFAELGEIAFVTRIRAILLRDTGAALSPFHSWLFLQGLETLSLRVERHIANT
HHHHHCCCCHHHHCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKIVDFLDKHPKVELVNHPLLESNAYHALYQKYYPKDAGSIFTFELKDKDEKKARDLIDH
HHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHH
LEIFSLLANVGDTKSLAIHPASTTHQQLNDEELASAGISKGTIRLSVGIEDVTDLIADLE
HHHHHHHHCCCCCCEEEEECCCCHHHHCCHHHHHHCCCCCCEEEEEECHHHHHHHHHHHH
QALEKI
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]