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The map label for this gene is pdhC [H]

Identifier: 125622951

GI number: 125622951

Start: 66910

End: 68502

Strand: Reverse

Name: pdhC [H]

Synonym: llmg_0072

Alternate gene names: 125622951

Gene position: 68502-66910 (Counterclockwise)

Preceding gene: 125622952

Following gene: 125622950

Centisome position: 2.71

GC content: 41.05

Gene sequence:

>1593_bases
ATGACTGAGATTTTTAAAATGCCTGATATTGGCGAAGGAATGCACGAAGGGGACATTGCCAACTGGTTAGTTAAAGTTGG
AGATGTTGTTAAAGAAGATGATCCAATTGCTGAAGTACAAAATGATAAACTCATGCAAGAAATTCTCTCTCCCTACTCTG
GGACTGTAACAAAACTCTTCGTTGAAGAAGGAACAACTGTAGAAGTTGATTCTCCTTTGGTTGAGTTTGATGGAGACGGG
TCAGGAACTTCTGCAGCTGCTCCTGCTCCTGCCGCTCAAGAAACAGTAAGTTCTGATGCTCCTTCTGGCAATGCTCAAAT
CTTCACCATGCCTGATATTGGTGAAGGAATGCATGAAGGCGATATTGCCAACTGGCTCGTTAAAGTTGGAGATGAAATTA
AAGAAGATGATCCTGTTGCAGAGGTTCAAAACGATAAACTCATGCAAGAGATTCTTTCTCCTTACTCTGGAAAAGTCACT
AAACTTTTCGTCGAAGCAGGAACAACTGTAGAAGTTGGGGCCCCTTTGATTGAATACAATGGAAATGGTGAATCTACAAG
TAATCCTGCTCCTGCTGCTAGTCCTGCTCCAATTGCGGAAGCTCCTAAAGCAGCCGCTGCACCTACTGATGCGCCATTGA
CAAAAACAACTTCTACTGGACATATTCTTGCTATGCCATCTGTCCGTCATTATGCTCGTAAAGCGGGAATTGATTTAACT
CAAGTACCTGCAACTGGTCGTCATGGGCATACAACTTTGGCTGACGTTAAAGCTTTTGAATCTGGTAGCGTTGCACCAAT
CGCTCCTGTGGCTCCAGAATCAGCTCCTGTGGCACCTGCGCCTAAAGCCGATAAAGCTACGGAAAAAGCCCCAACTGTTA
AATCTGTGGCTGGAGATCGTCGTGAAGCAATGAATCCAACTCGTAAAGTTGTTTCTAAAGTTATGACTGCTCAACACACT
CATATTCCACCAGTAACAAACTTTGATCAAGTGGAAGTTTCTAAACTTGTTAAACATCGCGCCGTCTTTAAAGAAGTAGC
AGCAAAACAAGATATCAAATTGACTTACCTTGCTTATGTTGCTAAAGCCTTGGCAACTACTGCTCATAAGTTCCCAGAAA
TTAATGCTTCTGTTGACTATGATAAGCAAGAAATTGTTTATCATGAACATGTCAACCTTGGGATTGCTGTAAATGCCCCA
ACTGGTTTATATGTCCCAGTCATTCATGAAGCTGAAACTAAATCAATTCTTGAAATTGCAAAAGAAATTGCTGAATTAGC
GACTGCTACACGTGAAGGAACACTTAAACCTCAACAAATGCAAGGTTCAACGATTACAATTTCAAATATTGGTTCTGCTC
GTGGTTCTTGGTTTACACCAATCATCAACGGTTCTGATGTTGTGATTTTAGGTTTGGGTTCAATTGTTAAAGAACCAATT
GTCAATGGTGAAGGTGAAATTGTCGTAGGACAAAACATGAAATTGTCTATGACTTATGACCACCGTTTGATTGATGGTAT
GTTGGGTCAAACATCACTTAACTACTTGAAATCACTTTTGGCTGACCCTGAGTTCATGCTTATGGAAATTTAA

Upstream 100 bases:

>100_bases
GTAACTGGTCCAGACTCAATTTTCCCATTTGCACAAGCGGAAAATGATTGGGCAGTTAAAGCTGAAGATATCGTGAATAA
AGTTAAAGAGGTGGTTGATT

Downstream 100 bases:

>100_bases
TACGATGTAATGAATGGTGCTGAATCCCCTATTCATTACACGCGAATTCCTAACGGACATTCGCTAATCGTCTTATTAGA
AAACTTATGAAAGAATATCG

Product: pyruvate dehydrogenase complex E2 component

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MTEIFKMPDIGEGMHEGDIANWLVKVGDVVKEDDPIAEVQNDKLMQEILSPYSGTVTKLFVEEGTTVEVDSPLVEFDGDG
SGTSAAAPAPAAQETVSSDAPSGNAQIFTMPDIGEGMHEGDIANWLVKVGDEIKEDDPVAEVQNDKLMQEILSPYSGKVT
KLFVEAGTTVEVGAPLIEYNGNGESTSNPAPAASPAPIAEAPKAAAAPTDAPLTKTTSTGHILAMPSVRHYARKAGIDLT
QVPATGRHGHTTLADVKAFESGSVAPIAPVAPESAPVAPAPKADKATEKAPTVKSVAGDRREAMNPTRKVVSKVMTAQHT
HIPPVTNFDQVEVSKLVKHRAVFKEVAAKQDIKLTYLAYVAKALATTAHKFPEINASVDYDKQEIVYHEHVNLGIAVNAP
TGLYVPVIHEAETKSILEIAKEIAELATATREGTLKPQQMQGSTITISNIGSARGSWFTPIINGSDVVILGLGSIVKEPI
VNGEGEIVVGQNMKLSMTYDHRLIDGMLGQTSLNYLKSLLADPEFMLMEI

Sequences:

>Translated_530_residues
MTEIFKMPDIGEGMHEGDIANWLVKVGDVVKEDDPIAEVQNDKLMQEILSPYSGTVTKLFVEEGTTVEVDSPLVEFDGDG
SGTSAAAPAPAAQETVSSDAPSGNAQIFTMPDIGEGMHEGDIANWLVKVGDEIKEDDPVAEVQNDKLMQEILSPYSGKVT
KLFVEAGTTVEVGAPLIEYNGNGESTSNPAPAASPAPIAEAPKAAAAPTDAPLTKTTSTGHILAMPSVRHYARKAGIDLT
QVPATGRHGHTTLADVKAFESGSVAPIAPVAPESAPVAPAPKADKATEKAPTVKSVAGDRREAMNPTRKVVSKVMTAQHT
HIPPVTNFDQVEVSKLVKHRAVFKEVAAKQDIKLTYLAYVAKALATTAHKFPEINASVDYDKQEIVYHEHVNLGIAVNAP
TGLYVPVIHEAETKSILEIAKEIAELATATREGTLKPQQMQGSTITISNIGSARGSWFTPIINGSDVVILGLGSIVKEPI
VNGEGEIVVGQNMKLSMTYDHRLIDGMLGQTSLNYLKSLLADPEFMLMEI
>Mature_529_residues
TEIFKMPDIGEGMHEGDIANWLVKVGDVVKEDDPIAEVQNDKLMQEILSPYSGTVTKLFVEEGTTVEVDSPLVEFDGDGS
GTSAAAPAPAAQETVSSDAPSGNAQIFTMPDIGEGMHEGDIANWLVKVGDEIKEDDPVAEVQNDKLMQEILSPYSGKVTK
LFVEAGTTVEVGAPLIEYNGNGESTSNPAPAASPAPIAEAPKAAAAPTDAPLTKTTSTGHILAMPSVRHYARKAGIDLTQ
VPATGRHGHTTLADVKAFESGSVAPIAPVAPESAPVAPAPKADKATEKAPTVKSVAGDRREAMNPTRKVVSKVMTAQHTH
IPPVTNFDQVEVSKLVKHRAVFKEVAAKQDIKLTYLAYVAKALATTAHKFPEINASVDYDKQEIVYHEHVNLGIAVNAPT
GLYVPVIHEAETKSILEIAKEIAELATATREGTLKPQQMQGSTITISNIGSARGSWFTPIINGSDVVILGLGSIVKEPIV
NGEGEIVVGQNMKLSMTYDHRLIDGMLGQTSLNYLKSLLADPEFMLMEI

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=436, Percent_Identity=32.1100917431193, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI19923748, Length=239, Percent_Identity=34.3096234309623, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI31711992, Length=583, Percent_Identity=26.9296740994854, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI203098816, Length=487, Percent_Identity=25.0513347022587, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI203098753, Length=448, Percent_Identity=24.7767857142857, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=28.75, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1786305, Length=534, Percent_Identity=30.1498127340824, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI1786946, Length=426, Percent_Identity=28.4037558685446, Blast_Score=160, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17537937, Length=431, Percent_Identity=32.9466357308585, Blast_Score=195, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI25146366, Length=428, Percent_Identity=30.3738317757009, Blast_Score=158, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI17560088, Length=454, Percent_Identity=27.9735682819383, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17538894, Length=316, Percent_Identity=26.5822784810127, Blast_Score=90, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320352, Length=422, Percent_Identity=31.2796208530806, Blast_Score=171, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6324258, Length=452, Percent_Identity=27.212389380531, Blast_Score=137, Evalue=6e-33,
Organism=Drosophila melanogaster, GI18859875, Length=427, Percent_Identity=32.0843091334895, Blast_Score=201, Evalue=8e-52,
Organism=Drosophila melanogaster, GI24582497, Length=226, Percent_Identity=28.3185840707965, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI20129315, Length=226, Percent_Identity=28.3185840707965, Blast_Score=112, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24645909, Length=232, Percent_Identity=33.6206896551724, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 56331; Mature: 56200

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00138 SUBTILASE_SER ; PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEIFKMPDIGEGMHEGDIANWLVKVGDVVKEDDPIAEVQNDKLMQEILSPYSGTVTKLF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
VEEGTTVEVDSPLVEFDGDGSGTSAAAPAPAAQETVSSDAPSGNAQIFTMPDIGEGMHEG
ECCCCEEEECCCEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCCCCC
DIANWLVKVGDEIKEDDPVAEVQNDKLMQEILSPYSGKVTKLFVEAGTTVEVGAPLIEYN
HHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEECCCEEEEC
GNGESTSNPAPAASPAPIAEAPKAAAAPTDAPLTKTTSTGHILAMPSVRHYARKAGIDLT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEECCHHHHHHHHHCCCEE
QVPATGRHGHTTLADVKAFESGSVAPIAPVAPESAPVAPAPKADKATEKAPTVKSVAGDR
ECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHCCH
REAMNPTRKVVSKVMTAQHTHIPPVTNFDQVEVSKLVKHRAVFKEVAAKQDIKLTYLAYV
HHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
AKALATTAHKFPEINASVDYDKQEIVYHEHVNLGIAVNAPTGLYVPVIHEAETKSILEIA
HHHHHHHHHHCCCCCCCCCCCHHHEEEEECCEEEEEEECCCCEEEEEEECCHHHHHHHHH
KEIAELATATREGTLKPQQMQGSTITISNIGSARGSWFTPIINGSDVVILGLGSIVKEPI
HHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCCCCCEECEEECCCCEEEEECHHHHHHHC
VNGEGEIVVGQNMKLSMTYDHRLIDGMLGQTSLNYLKSLLADPEFMLMEI
CCCCCCEEECCCEEEEEEECHHHHHHHHCHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure 
TEIFKMPDIGEGMHEGDIANWLVKVGDVVKEDDPIAEVQNDKLMQEILSPYSGTVTKLF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
VEEGTTVEVDSPLVEFDGDGSGTSAAAPAPAAQETVSSDAPSGNAQIFTMPDIGEGMHEG
ECCCCEEEECCCEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCCCCC
DIANWLVKVGDEIKEDDPVAEVQNDKLMQEILSPYSGKVTKLFVEAGTTVEVGAPLIEYN
HHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEECCCEEEEC
GNGESTSNPAPAASPAPIAEAPKAAAAPTDAPLTKTTSTGHILAMPSVRHYARKAGIDLT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEECCHHHHHHHHHCCCEE
QVPATGRHGHTTLADVKAFESGSVAPIAPVAPESAPVAPAPKADKATEKAPTVKSVAGDR
ECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHCCH
REAMNPTRKVVSKVMTAQHTHIPPVTNFDQVEVSKLVKHRAVFKEVAAKQDIKLTYLAYV
HHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
AKALATTAHKFPEINASVDYDKQEIVYHEHVNLGIAVNAPTGLYVPVIHEAETKSILEIA
HHHHHHHHHHCCCCCCCCCCCHHHEEEEECCEEEEEEECCCCEEEEEEECCHHHHHHHHH
KEIAELATATREGTLKPQQMQGSTITISNIGSARGSWFTPIINGSDVVILGLGSIVKEPI
HHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCCCCCEECEEECCCCEEEEECHHHHHHHC
VNGEGEIVVGQNMKLSMTYDHRLIDGMLGQTSLNYLKSLLADPEFMLMEI
CCCCCCEEECCCEEEEEEECHHHHHHHHCHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377 [H]