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The map label for this gene is infB

Identifier: 124486335

GI number: 124486335

Start: 1552065

End: 1553834

Strand: Reverse

Name: infB

Synonym: Mlab_1520

Alternate gene names: 124486335

Gene position: 1553834-1552065 (Counterclockwise)

Preceding gene: 124486336

Following gene: 124486334

Centisome position: 86.09

GC content: 53.39

Gene sequence:

>1770_bases
ATGGAAGAACACATCAGAACTCCCATCGTCTGCGTTTTGGGGCACGTAGATCATGGAAAAACATCGCTCCTCGACCGTAT
TCGCGGGTCGAAAGTTGTTGCAGGCGAAGCAGGGGCGATTACCCAGCATATCGGCGCTACTCTCATTCCGTTTGATTCTA
TTGCAAAAATGAGCGGCGATCTGGGCAGGCTCAAAACCTCTGTTCCGGGACTGCTGTTTATCGACACGCCCGGTCATCAT
GCATTTACCACGCTTCGGGCCAGGGGAGGTGCTCTTGCCGATATCGCGATTTTGGTTGTGGATGTGAATGAAGGGTTCAA
ACAGCAGACGATCGAAGCGCTTCAGATCCTCCGGACCTGTAAAACGCCGTTTGTTATCGCAGCAACCAAACTCGACAAGA
TTCCCGGCTGGCGTCCGACACCGAACGCTTCGTTTCAGAAAGCTTACAAAAATCAGAGTGAACGTGTCCAGACGGAGTGC
GAAAACAGGGTATATGAACTTGTCGGCAAGCTTTCGGATATGGGTTTCAACTCCGAGCGGTTTGACCGGGTGAGTGATTT
CCAGCGCAATCTGGTGATCGTTCCGGTGAGCAGCATGACCGGTGAAGGTATCGGCGATCTTTTGATGATCATGATCGGAC
TTGCCCAGCGGTATCTCACCGAAGGACTCAAGACGACCACATCGGGCCCGGGTGTTGGAACTGTCCTTGAGGTCAAGGAA
GAGAAAGGTCTTGGAACGACACTCGACGTTATCCTTTATGATGGGATCATCAGTGTGGGCGACGAGATCGGTATTGCCGG
CAGTGATGGGGCACTGTCGACCAAAGTCCGCGCTCTTCTCCAGCCCCGGCCCATGAAAGAGATCTTAATCGAAGATCAGT
TTATGCGGGTCAAATCCGTCGTGGCGGCGGCCGGTGTGAAAATTTCCGCGCCGAATCTGGAATCGATCGTTGCCGGCTCG
CCGATCCGCGTGATACGCGGTGATCACGACGAGGTTCTGGCAAAGATCAACGAGGAGATGCAGGAGATCAACATAAAACT
CTCCGATGTAGGGGTAAGCGTCCGTGCCGACACGATCGGTGCACTGGAGGCCCTTTCCAATGAACTGGATGCGAAAAACA
TCCCGATTATGCGTGCGAGCGTAGGTCCCTTGTCCCGCCGTGATCTGATCGATATCAGCGTGATAAAGGAGGATCTGTTC
AAAGTTGCTCTCTGTTTCAACGTTCCTCTCCTTCCCGATGCCGAAGCGATGGTCCGGGACGAGGAGGTGGATGTGAAGAT
CTTTTCCAATCGGGTCATCTACAAACTCCTTGATGATTATCTGGAATGGCGCGATGAAATGATTCGGGCCAAAGAAGCGA
AACAGTTCGAGACGGTCGTTCTTCCGGCGAAGTTTTCCATCCTCCCCGGTTGTGTTTTCAGGATGAGCGGTCCGGCGGTC
GTCGGCGTGCGCGTTTTAGGGGGTACGCTTCGTCCGAAGGTCAGCATCGCGACCCGCGACGGAAAAATCGTCGGCGAGAT
CAAACAGATCAAGCTCAACAAAGAGACCATTGGCGAGGCAAAAGAGGGCGCAGAGGTCGCCGTTTCGATAGATGGGGTCA
CGATCGGCAGACAGATCGATGTCGGCGAAACACTTTATGTGGCGATTCCTGAGCGGCATGTGAAGGTTTTGGAGACGGAA
ATGCTCTCTCATTTAAATGCTGGCACCGTCGAAGCACTCGAAGAGTACACCGGCATCTTCCGGAAAACACAGCCTTTCTG
GGGCAAGTAG

Upstream 100 bases:

>100_bases
TTTTTCTTAGTCCCCTTTATATTAATAATCCTGCACTGCCAATAGATAATCACTATTTATCTCTGTATGGTCAGTCATCG
GAGAATGGGGATTCAATATC

Downstream 100 bases:

>100_bases
TATAAATGTGGAGATACTAAATATATGGGTACTTCGGCATCATAGATATCGAGGAGATGTGTATATACAATGGTAGACTT
TAAGGTCGTACTGTCAGACC

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MEEHIRTPIVCVLGHVDHGKTSLLDRIRGSKVVAGEAGAITQHIGATLIPFDSIAKMSGDLGRLKTSVPGLLFIDTPGHH
AFTTLRARGGALADIAILVVDVNEGFKQQTIEALQILRTCKTPFVIAATKLDKIPGWRPTPNASFQKAYKNQSERVQTEC
ENRVYELVGKLSDMGFNSERFDRVSDFQRNLVIVPVSSMTGEGIGDLLMIMIGLAQRYLTEGLKTTTSGPGVGTVLEVKE
EKGLGTTLDVILYDGIISVGDEIGIAGSDGALSTKVRALLQPRPMKEILIEDQFMRVKSVVAAAGVKISAPNLESIVAGS
PIRVIRGDHDEVLAKINEEMQEINIKLSDVGVSVRADTIGALEALSNELDAKNIPIMRASVGPLSRRDLIDISVIKEDLF
KVALCFNVPLLPDAEAMVRDEEVDVKIFSNRVIYKLLDDYLEWRDEMIRAKEAKQFETVVLPAKFSILPGCVFRMSGPAV
VGVRVLGGTLRPKVSIATRDGKIVGEIKQIKLNKETIGEAKEGAEVAVSIDGVTIGRQIDVGETLYVAIPERHVKVLETE
MLSHLNAGTVEALEEYTGIFRKTQPFWGK

Sequences:

>Translated_589_residues
MEEHIRTPIVCVLGHVDHGKTSLLDRIRGSKVVAGEAGAITQHIGATLIPFDSIAKMSGDLGRLKTSVPGLLFIDTPGHH
AFTTLRARGGALADIAILVVDVNEGFKQQTIEALQILRTCKTPFVIAATKLDKIPGWRPTPNASFQKAYKNQSERVQTEC
ENRVYELVGKLSDMGFNSERFDRVSDFQRNLVIVPVSSMTGEGIGDLLMIMIGLAQRYLTEGLKTTTSGPGVGTVLEVKE
EKGLGTTLDVILYDGIISVGDEIGIAGSDGALSTKVRALLQPRPMKEILIEDQFMRVKSVVAAAGVKISAPNLESIVAGS
PIRVIRGDHDEVLAKINEEMQEINIKLSDVGVSVRADTIGALEALSNELDAKNIPIMRASVGPLSRRDLIDISVIKEDLF
KVALCFNVPLLPDAEAMVRDEEVDVKIFSNRVIYKLLDDYLEWRDEMIRAKEAKQFETVVLPAKFSILPGCVFRMSGPAV
VGVRVLGGTLRPKVSIATRDGKIVGEIKQIKLNKETIGEAKEGAEVAVSIDGVTIGRQIDVGETLYVAIPERHVKVLETE
MLSHLNAGTVEALEEYTGIFRKTQPFWGK
>Mature_589_residues
MEEHIRTPIVCVLGHVDHGKTSLLDRIRGSKVVAGEAGAITQHIGATLIPFDSIAKMSGDLGRLKTSVPGLLFIDTPGHH
AFTTLRARGGALADIAILVVDVNEGFKQQTIEALQILRTCKTPFVIAATKLDKIPGWRPTPNASFQKAYKNQSERVQTEC
ENRVYELVGKLSDMGFNSERFDRVSDFQRNLVIVPVSSMTGEGIGDLLMIMIGLAQRYLTEGLKTTTSGPGVGTVLEVKE
EKGLGTTLDVILYDGIISVGDEIGIAGSDGALSTKVRALLQPRPMKEILIEDQFMRVKSVVAAAGVKISAPNLESIVAGS
PIRVIRGDHDEVLAKINEEMQEINIKLSDVGVSVRADTIGALEALSNELDAKNIPIMRASVGPLSRRDLIDISVIKEDLF
KVALCFNVPLLPDAEAMVRDEEVDVKIFSNRVIYKLLDDYLEWRDEMIRAKEAKQFETVVLPAKFSILPGCVFRMSGPAV
VGVRVLGGTLRPKVSIATRDGKIVGEIKQIKLNKETIGEAKEGAEVAVSIDGVTIGRQIDVGETLYVAIPERHVKVLETE
MLSHLNAGTVEALEEYTGIFRKTQPFWGK

Specific function: Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family

Homologues:

Organism=Homo sapiens, GI84043963, Length=595, Percent_Identity=35.1260504201681, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI53729339, Length=589, Percent_Identity=26.4855687606112, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI53729337, Length=589, Percent_Identity=26.4855687606112, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1789559, Length=577, Percent_Identity=28.9428076256499, Blast_Score=142, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI212656558, Length=566, Percent_Identity=36.5724381625442, Blast_Score=350, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI71994658, Length=146, Percent_Identity=42.4657534246575, Blast_Score=115, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6319282, Length=581, Percent_Identity=37.5215146299484, Blast_Score=362, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6324550, Length=581, Percent_Identity=27.8829604130809, Blast_Score=148, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24656849, Length=571, Percent_Identity=35.7267950963222, Blast_Score=341, Evalue=7e-94,
Organism=Drosophila melanogaster, GI28572034, Length=547, Percent_Identity=27.0566727605119, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI160714833, Length=84, Percent_Identity=50, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): IF2P_METLZ (A2STM8)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001030951.1
- ProteinModelPortal:   A2STM8
- SMR:   A2STM8
- STRING:   A2STM8
- GeneID:   4794572
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_1520
- eggNOG:   arNOG04413
- HOGENOM:   HBG635083
- OMA:   SPLRIVP
- PhylomeDB:   A2STM8
- ProtClustDB:   PRK04004
- BioCyc:   MLAB410358:MLAB_1520-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00100_A
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004544
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.40.50.10050
- PANTHER:   PTHR23115:SF41
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00491
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; SSF52156 TIF_IF2_dom3; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 64163; Mature: 64163

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEHIRTPIVCVLGHVDHGKTSLLDRIRGSKVVAGEAGAITQHIGATLIPFDSIAKMSGD
CCCCCCCCEEEEEECCCCCHHHHHHHHCCCEEEECCCCHHHHHHCCEECCHHHHHHHCCC
LGRLKTSVPGLLFIDTPGHHAFTTLRARGGALADIAILVVDVNEGFKQQTIEALQILRTC
HHHHHHCCCCEEEEECCCCCCEEEEHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHC
KTPFVIAATKLDKIPGWRPTPNASFQKAYKNQSERVQTECENRVYELVGKLSDMGFNSER
CCCEEEEEEHHHCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
FDRVSDFQRNLVIVPVSSMTGEGIGDLLMIMIGLAQRYLTEGLKTTTSGPGVGTVLEVKE
HHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEC
EKGLGTTLDVILYDGIISVGDEIGIAGSDGALSTKVRALLQPRPMKEILIEDQFMRVKSV
CCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VAAAGVKISAPNLESIVAGSPIRVIRGDHDEVLAKINEEMQEINIKLSDVGVSVRADTIG
HHHCCCEEECCCHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHEEEEEECCEEEEHHHHH
ALEALSNELDAKNIPIMRASVGPLSRRDLIDISVIKEDLFKVALCFNVPLLPDAEAMVRD
HHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCC
EEVDVKIFSNRVIYKLLDDYLEWRDEMIRAKEAKQFETVVLPAKFSILPGCVFRMSGPAV
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHCCCEEEEECCCEE
VGVRVLGGTLRPKVSIATRDGKIVGEIKQIKLNKETIGEAKEGAEVAVSIDGVTIGRQID
EEEEEECCCCCCEEEEEECCCCEEEEHHHEEECHHHHCCCCCCCEEEEEECCEEECCEEC
VGETLYVAIPERHVKVLETEMLSHLNAGTVEALEEYTGIFRKTQPFWGK
CCCEEEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEEHIRTPIVCVLGHVDHGKTSLLDRIRGSKVVAGEAGAITQHIGATLIPFDSIAKMSGD
CCCCCCCCEEEEEECCCCCHHHHHHHHCCCEEEECCCCHHHHHHCCEECCHHHHHHHCCC
LGRLKTSVPGLLFIDTPGHHAFTTLRARGGALADIAILVVDVNEGFKQQTIEALQILRTC
HHHHHHCCCCEEEEECCCCCCEEEEHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHC
KTPFVIAATKLDKIPGWRPTPNASFQKAYKNQSERVQTECENRVYELVGKLSDMGFNSER
CCCEEEEEEHHHCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
FDRVSDFQRNLVIVPVSSMTGEGIGDLLMIMIGLAQRYLTEGLKTTTSGPGVGTVLEVKE
HHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEC
EKGLGTTLDVILYDGIISVGDEIGIAGSDGALSTKVRALLQPRPMKEILIEDQFMRVKSV
CCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VAAAGVKISAPNLESIVAGSPIRVIRGDHDEVLAKINEEMQEINIKLSDVGVSVRADTIG
HHHCCCEEECCCHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHEEEEEECCEEEEHHHHH
ALEALSNELDAKNIPIMRASVGPLSRRDLIDISVIKEDLFKVALCFNVPLLPDAEAMVRD
HHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCC
EEVDVKIFSNRVIYKLLDDYLEWRDEMIRAKEAKQFETVVLPAKFSILPGCVFRMSGPAV
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHCCCEEEEECCCEE
VGVRVLGGTLRPKVSIATRDGKIVGEIKQIKLNKETIGEAKEGAEVAVSIDGVTIGRQID
EEEEEECCCCCCEEEEEECCCCEEEEHHHEEECHHHHCCCCCCCEEEEEECCEEECCEEC
VGETLYVAIPERHVKVLETEMLSHLNAGTVEALEEYTGIFRKTQPFWGK
CCCEEEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA