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The map label for this gene is ahpF [H]

Identifier: 124486316

GI number: 124486316

Start: 1534467

End: 1535663

Strand: Reverse

Name: ahpF [H]

Synonym: Mlab_1501

Alternate gene names: 124486316

Gene position: 1535663-1534467 (Counterclockwise)

Preceding gene: 124486317

Following gene: 124486306

Centisome position: 85.08

GC content: 47.45

Gene sequence:

>1197_bases
ATGTTACATGAAGTGGTTGTGTACTCGCTTTCAGGTTGTCCTCACTGTAAAGCATTGAAAACATTCCTTGATAACCAGAA
TATAACGTACACCAATATCGATGTAGGCGAAGATGAAAAAGCCGCTGCTGAAATGATAAAGATCTCCGGCCAGAGGGGGG
TTCCGGTCACGGTCATCGACGGCGAAAAAATCGTTATTGGCGATGATTTGAAAAAAGTCATGGAGTATCTTGACGCTCCG
GTTGCGGTAAAAAAGACTCCGGATGTTTCTGCAGATCATGATCTCGTAGTGATCGGTGCCGGAGCCGGCGGTCTGTCTGC
AGCCATGTACGGCGCCCGAAAAGGCATCGATATGGTCGTGATCACCGGTGCTATTGGAGGAATGGTGAATCAGAGTTATG
TTGTTGAAAACTATCCCGGCATCCCTGATATATCCGGTGCTGATCTGATGAAAAAAATCTATGATCATACGGTAAATTCA
GGTGGGATTTTCGTTGAGGATGTTGTGACCGGCATTTCCAAAGCCGGGGATGTTTTTTCCATTGACACGCTTAACGGAGC
CCATTATACTGCAAAAGCCGTCATAGCGGCCACTGGAAGGTCTCCACGTCTCTCCGGAGCGAAAGGCGAAACAGACTATC
TCGGAAAAGGCGTTGCGATCTGTACGACCTGTGACGGGCCGTTGTACAAAAATAAAGTGGTCGGGATCCTTGGGGGGGGT
AACACGGCAGTTGATATGGCGATCGAACTGAGCGATATTGCATCAAAAATCCATCTGATCGTCCGCAGTAAGCTGAAAGC
CGACAAGGTTCTGATCGACCGGCTCAAAACGAAGAAGAACATTGTTCTGCACAAAGGATATACTATCGAAGAATTTGGCG
GCGGTCAGTTTCTGGAATATGTGATTTTGAAAAAACAGGGCGGGATCACCAGCCTCCTTGGAAAAGGTGAAGAAAAGATC
CCTGTCGACGGCGTCTTCCTTGGGATCGGTCTTGATCCAAACACCTCCATCTTTGAGGGTCTTGGAGTAACAATGAATCC
AAATAAAGAGATCATCGTCGATATCGACTGCAATACGAATGTTCCCGGATTCTATGCGGCCGGAGATGCGACCTCCATCA
AAGCCAAGCAGATCGCATCATCCGTTGGTGAAGGAGTAAAGGCTCTTCTTTCAGCATATGATTACCTGAAAAGGTAA

Upstream 100 bases:

>100_bases
TCAGACACCGTGAAAATCAAAAAATATAATTAACGCCCTTTTTCACACATTTTTTATCAGGAACCAATAGTATTAATCCT
TTGAGTGCAGACTCATTGTT

Downstream 100 bases:

>100_bases
CTCTCTTTTTTTCAAAATATAGTCCGTCTCTGGATCTGAGATAACTCTCTCAGAATCGGATCATTGAAAACATTCAGATA
AAAAAGATTGGCTGAGAATA

Product: hypothetical protein

Products: NADP+; reduced thioredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MLHEVVVYSLSGCPHCKALKTFLDNQNITYTNIDVGEDEKAAAEMIKISGQRGVPVTVIDGEKIVIGDDLKKVMEYLDAP
VAVKKTPDVSADHDLVVIGAGAGGLSAAMYGARKGIDMVVITGAIGGMVNQSYVVENYPGIPDISGADLMKKIYDHTVNS
GGIFVEDVVTGISKAGDVFSIDTLNGAHYTAKAVIAATGRSPRLSGAKGETDYLGKGVAICTTCDGPLYKNKVVGILGGG
NTAVDMAIELSDIASKIHLIVRSKLKADKVLIDRLKTKKNIVLHKGYTIEEFGGGQFLEYVILKKQGGITSLLGKGEEKI
PVDGVFLGIGLDPNTSIFEGLGVTMNPNKEIIVDIDCNTNVPGFYAAGDATSIKAKQIASSVGEGVKALLSAYDYLKR

Sequences:

>Translated_398_residues
MLHEVVVYSLSGCPHCKALKTFLDNQNITYTNIDVGEDEKAAAEMIKISGQRGVPVTVIDGEKIVIGDDLKKVMEYLDAP
VAVKKTPDVSADHDLVVIGAGAGGLSAAMYGARKGIDMVVITGAIGGMVNQSYVVENYPGIPDISGADLMKKIYDHTVNS
GGIFVEDVVTGISKAGDVFSIDTLNGAHYTAKAVIAATGRSPRLSGAKGETDYLGKGVAICTTCDGPLYKNKVVGILGGG
NTAVDMAIELSDIASKIHLIVRSKLKADKVLIDRLKTKKNIVLHKGYTIEEFGGGQFLEYVILKKQGGITSLLGKGEEKI
PVDGVFLGIGLDPNTSIFEGLGVTMNPNKEIIVDIDCNTNVPGFYAAGDATSIKAKQIASSVGEGVKALLSAYDYLKR
>Mature_398_residues
MLHEVVVYSLSGCPHCKALKTFLDNQNITYTNIDVGEDEKAAAEMIKISGQRGVPVTVIDGEKIVIGDDLKKVMEYLDAP
VAVKKTPDVSADHDLVVIGAGAGGLSAAMYGARKGIDMVVITGAIGGMVNQSYVVENYPGIPDISGADLMKKIYDHTVNS
GGIFVEDVVTGISKAGDVFSIDTLNGAHYTAKAVIAATGRSPRLSGAKGETDYLGKGVAICTTCDGPLYKNKVVGILGGG
NTAVDMAIELSDIASKIHLIVRSKLKADKVLIDRLKTKKNIVLHKGYTIEEFGGGQFLEYVILKKQGGITSLLGKGEEKI
PVDGVFLGIGLDPNTSIFEGLGVTMNPNKEIIVDIDCNTNVPGFYAAGDATSIKAKQIASSVGEGVKALLSAYDYLKR

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG0492

COG function: function code O; Thioredoxin reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787114, Length=312, Percent_Identity=38.1410256410256, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI87081763, Length=409, Percent_Identity=33.7408312958435, Blast_Score=171, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6320560, Length=319, Percent_Identity=31.0344827586207, Blast_Score=142, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6321898, Length=318, Percent_Identity=29.874213836478, Blast_Score=139, Evalue=1e-33,

Paralogues:

None

Copy number: 808 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.8.1.9 [C]

Molecular weight: Translated: 41928; Mature: 41928

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00573 PYRIDINE_REDOX_2 ; PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHEVVVYSLSGCPHCKALKTFLDNQNITYTNIDVGEDEKAAAEMIKISGQRGVPVTVID
CCCEEEEEECCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEEC
GEKIVIGDDLKKVMEYLDAPVAVKKTPDVSADHDLVVIGAGAGGLSAAMYGARKGIDMVV
CCEEEECHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEE
ITGAIGGMVNQSYVVENYPGIPDISGADLMKKIYDHTVNSGGIFVEDVVTGISKAGDVFS
EECCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCCEEE
IDTLNGAHYTAKAVIAATGRSPRLSGAKGETDYLGKGVAICTTCDGPLYKNKVVGILGGG
EECCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECC
NTAVDMAIELSDIASKIHLIVRSKLKADKVLIDRLKTKKNIVLHKGYTIEEFGGGQFLEY
CEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEECCCCCEEEE
VILKKQGGITSLLGKGEEKIPVDGVFLGIGLDPNTSIFEGLGVTMNPNKEIIVDIDCNTN
EEEECCCCHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHCCCEEECCCCEEEEEEECCCC
VPGFYAAGDATSIKAKQIASSVGEGVKALLSAYDYLKR
CCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLHEVVVYSLSGCPHCKALKTFLDNQNITYTNIDVGEDEKAAAEMIKISGQRGVPVTVID
CCCEEEEEECCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEEC
GEKIVIGDDLKKVMEYLDAPVAVKKTPDVSADHDLVVIGAGAGGLSAAMYGARKGIDMVV
CCEEEECHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEE
ITGAIGGMVNQSYVVENYPGIPDISGADLMKKIYDHTVNSGGIFVEDVVTGISKAGDVFS
EECCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCCEEE
IDTLNGAHYTAKAVIAATGRSPRLSGAKGETDYLGKGVAICTTCDGPLYKNKVVGILGGG
EECCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECC
NTAVDMAIELSDIASKIHLIVRSKLKADKVLIDRLKTKKNIVLHKGYTIEEFGGGQFLEY
CEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEECCCCCEEEE
VILKKQGGITSLLGKGEEKIPVDGVFLGIGLDPNTSIFEGLGVTMNPNKEIIVDIDCNTN
EEEECCCCHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHCCCEEECCCCEEEEEEECCCC
VPGFYAAGDATSIKAKQIASSVGEGVKALLSAYDYLKR
CCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: NA

Kcat value (1/min): 1365 [C]

Specific activity: NA

Km value (mM): 0.008 {NADPH}} 0.125 {thioredoxin} 0.002 {thioredoxin}} 0.0017 {thioredoxin}} [C]

Substrates: NADPH; oxidized thioredoxin [C]

Specific reaction: NADPH + oxidized thioredoxin = NADP+ + reduced thioredoxin [C]

General reaction: Redox reaction [C]

Inhibitor: Ca2+; N-Ethyl maleimide; NADP+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA