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The map label for this gene is rnhB

Identifier: 124486303

GI number: 124486303

Start: 1522721

End: 1523359

Strand: Reverse

Name: rnhB

Synonym: Mlab_1488

Alternate gene names: 124486303

Gene position: 1523359-1522721 (Counterclockwise)

Preceding gene: 124486305

Following gene: 124486302

Centisome position: 84.4

GC content: 51.8

Gene sequence:

>639_bases
ATGACAATCTGTGGGGTCGATGAGGCGGGGAAAGGTCCGGTTCTCGGTCCGATGATCACCGCAGGTGTTCTTGTATCGGA
TATGTCCGAACTGGAGATGCTTGGCATCAAAGACTCCAAAAAACTCAGCCCGAAAAAACGCGAGAGCCTGTTTGAAGAGA
TCACTTTTTCATGGAAGACATATACGGTCGTGCGGACTCCTTTTGATATCGATTCACGAGAGGGGACGATGAATGCATTT
ACGGCTTCCTGCCATGCAGATGTTGTCCGTGCTCTCTGCGCTGATTTTGTGTATCTTGATGCCTGCGATGTGAATGCAAA
ACGTTTCGGCGAAAATGTTCTCAGGCTGAGCGGGAGTTCCGCTCACGTATGTTCCGAGCACAAAGCCGATGCCAAATATG
CGGTTGTCGGGGCGGCAAGCATCGTCGCAAAAGTTACCCGGGACAGATGTATAGCCGACCTCAAGGAAGAATACGGCGAG
ATCGGGAGCGGATATCCCTCGGATCCGGCAACGATCTCTTTTCTCACTGAGTATATACGTACACGCGGGGAAGTTCCGCT
CTGCGCCCGCAGATCGTGGCAGACCGTACAGGATATCCTTGATCGGGCATCACAGACAGGTCTCTCTGACTTCTTCTAA

Upstream 100 bases:

>100_bases
GACTTCTTCCAATTCCGCCCAGACCAACGATGATGAGATACATAGGAAATCGTATGCGTTCAGGTGGTATTATATCTATT
CCCTGAAATATACGTATCCG

Downstream 100 bases:

>100_bases
GCAACCTCTTAACATCCGACCTCCAATAAGTATAGAATGGACTTGAGCTGGCTTATTCCGGTACTCATTGCAGTGATGAT
TTATGCCTGTATATGTTATG

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MTICGVDEAGKGPVLGPMITAGVLVSDMSELEMLGIKDSKKLSPKKRESLFEEITFSWKTYTVVRTPFDIDSREGTMNAF
TASCHADVVRALCADFVYLDACDVNAKRFGENVLRLSGSSAHVCSEHKADAKYAVVGAASIVAKVTRDRCIADLKEEYGE
IGSGYPSDPATISFLTEYIRTRGEVPLCARRSWQTVQDILDRASQTGLSDFF

Sequences:

>Translated_212_residues
MTICGVDEAGKGPVLGPMITAGVLVSDMSELEMLGIKDSKKLSPKKRESLFEEITFSWKTYTVVRTPFDIDSREGTMNAF
TASCHADVVRALCADFVYLDACDVNAKRFGENVLRLSGSSAHVCSEHKADAKYAVVGAASIVAKVTRDRCIADLKEEYGE
IGSGYPSDPATISFLTEYIRTRGEVPLCARRSWQTVQDILDRASQTGLSDFF
>Mature_211_residues
TICGVDEAGKGPVLGPMITAGVLVSDMSELEMLGIKDSKKLSPKKRESLFEEITFSWKTYTVVRTPFDIDSREGTMNAFT
ASCHADVVRALCADFVYLDACDVNAKRFGENVLRLSGSSAHVCSEHKADAKYAVVGAASIVAKVTRDRCIADLKEEYGEI
GSGYPSDPATISFLTEYIRTRGEVPLCARRSWQTVQDILDRASQTGLSDFF

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=207, Percent_Identity=30.4347826086957, Blast_Score=72, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324257, Length=232, Percent_Identity=35.7758620689655, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24580635, Length=231, Percent_Identity=35.0649350649351, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_METLZ (A2STJ6)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001030919.1
- ProteinModelPortal:   A2STJ6
- SMR:   A2STJ6
- STRING:   A2STJ6
- GeneID:   4795940
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_1488
- eggNOG:   arNOG07713
- HOGENOM:   HBG584843
- OMA:   AEHKADE
- PhylomeDB:   A2STJ6
- ProtClustDB:   PRK00015
- BioCyc:   MLAB410358:MLAB_1488-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_A
- InterPro:   IPR004649
- InterPro:   IPR001352
- InterPro:   IPR020787
- InterPro:   IPR023160
- InterPro:   IPR012337
- Gene3D:   G3DSA:1.10.10.460
- PANTHER:   PTHR10954
- TIGRFAMs:   TIGR00729

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 23182; Mature: 23050

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTICGVDEAGKGPVLGPMITAGVLVSDMSELEMLGIKDSKKLSPKKRESLFEEITFSWKT
CEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEE
YTVVRTPFDIDSREGTMNAFTASCHADVVRALCADFVYLDACDVNAKRFGENVLRLSGSS
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCC
AHVCSEHKADAKYAVVGAASIVAKVTRDRCIADLKEEYGEIGSGYPSDPATISFLTEYIR
CHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
TRGEVPLCARRSWQTVQDILDRASQTGLSDFF
CCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TICGVDEAGKGPVLGPMITAGVLVSDMSELEMLGIKDSKKLSPKKRESLFEEITFSWKT
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEE
YTVVRTPFDIDSREGTMNAFTASCHADVVRALCADFVYLDACDVNAKRFGENVLRLSGSS
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCC
AHVCSEHKADAKYAVVGAASIVAKVTRDRCIADLKEEYGEIGSGYPSDPATISFLTEYIR
CHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
TRGEVPLCARRSWQTVQDILDRASQTGLSDFF
CCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA