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The map label for this gene is paaK [H]

Identifier: 124486273

GI number: 124486273

Start: 1490873

End: 1492159

Strand: Reverse

Name: paaK [H]

Synonym: Mlab_1457

Alternate gene names: 124486273

Gene position: 1492159-1490873 (Counterclockwise)

Preceding gene: 124486274

Following gene: 124486272

Centisome position: 82.67

GC content: 52.45

Gene sequence:

>1287_bases
ATGTTCTGGAATGAGAAGATGGAAACCCTGTCCGGCCAGGCTCTTGAAGAGCTTCAGCTCAAACGTCTCAAGGAGACGGT
TGCACGGACGCAGAATATCGGTTTCTACAATACCCTGTTCAAAGATGCGGGAATAACCCCGTCTGATATTAAATCACTAG
ATGATCTGGCAAAGATTCCGTTCACGAAGAAAGCGGATCTCCGTGGGGGATATCCGTTTGGCTTTTTAGCGGTGCCAATG
AATCAGGTCAACCGTATTCATACAACATCCGGAACGACCGGGAAACCGACGGTCGTTGCCTATACGAAAAACGATCTCAA
TATGTGGTCGGAACTTATCGCACGGAACCTGACGATGGTGGGGCTTAGAGCCGGCGATATTTTCCAGAATGCATCGAACT
ACTCTCTTTTTACCGGCGGGCTCGGCATCCATATGGGGGCTGAGAAGATCGGCTGCGCTGTTGTGCCGTCAGGCGTCGGC
AATACAAAACGGCAGATCGAGATGATCCAGGATTTCAAGGTCAAGGGTCTTCACTGCACGCCGAGCTATGCGATGCATCT
GACCGAGGTGGCTGAGGAAATGCATGCGGATCTTGCTTCGCTTGAGATCGGCTGTTTTGGTGCGGAAGCCTGGTCGGAGA
ATATGCGCCGCGATCTGGAGAACCGTCTCGGTCTCAAGGCCTACGACAGTTACGGGATGAGTGAACTTTTCGGTCCGGGC
GTTGCCTTTGAATGCCCCGAACAGGACGGTCTGCATATCTGGCACGACTGTTATATCGTCGAGATCATCGATCCTAAGAC
CGGCGAGGTGCTCGGTCCCGAAGAGAAGGGAGAAATGGTCGTGACCCCGATCGTGAAGGAGGCCATGCCTCTCTTAAGAT
ACAGGACCGGCGATATCACGATGCTCATGGAGGATGAGTGTCCGTGCGGCCGTGGTCAGAAGATCGCAAGACTGCTTGGA
AGAAGCGATGATATGCTGACGGTCCGTGGGATCAATGTTTTCCCGAGTCAGATCGAGCATGTACTGAAAAACATCCCCGA
AGTGGGCGATCAGTTCATAGTCTACATCGATCGGGTGAACCATCTTGATGAGATGATGATCGAGGTGGAGATGAATAAAA
ACATCTTCTCGGGAGAACTTCAGGATCTCTCGAAGCTGCAGACGAAGATCATGAAGGCCCTGCAGGACACGCTTACGCTG
CGTGCCAGAGTCGAGCTTGTAGAGCCGGGGTCTCTCCCCCGCTTTGAAGGGAAAGCAAAGAGAGTCGTTGACCGGAGGGT
CATTTAA

Upstream 100 bases:

>100_bases
CAAGTAATAACCATTATGTTCGTATAAAACAATGTAATAGGGTGCTAAAGTGTAGCATGACACACCGTGTCATGCCCCCA
TCTGCAGAGGACTTAACCAT

Downstream 100 bases:

>100_bases
GTATGGCGTATTATAACGAAAAGATCGAAACGATGCCCAAAGATGAGCTTTCGAAGCTCCAGTATCAGGAACTGAAAAAA
CTGGTCACGCGTCTCTACGA

Product: phenylacetate-CoA ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MFWNEKMETLSGQALEELQLKRLKETVARTQNIGFYNTLFKDAGITPSDIKSLDDLAKIPFTKKADLRGGYPFGFLAVPM
NQVNRIHTTSGTTGKPTVVAYTKNDLNMWSELIARNLTMVGLRAGDIFQNASNYSLFTGGLGIHMGAEKIGCAVVPSGVG
NTKRQIEMIQDFKVKGLHCTPSYAMHLTEVAEEMHADLASLEIGCFGAEAWSENMRRDLENRLGLKAYDSYGMSELFGPG
VAFECPEQDGLHIWHDCYIVEIIDPKTGEVLGPEEKGEMVVTPIVKEAMPLLRYRTGDITMLMEDECPCGRGQKIARLLG
RSDDMLTVRGINVFPSQIEHVLKNIPEVGDQFIVYIDRVNHLDEMMIEVEMNKNIFSGELQDLSKLQTKIMKALQDTLTL
RARVELVEPGSLPRFEGKAKRVVDRRVI

Sequences:

>Translated_428_residues
MFWNEKMETLSGQALEELQLKRLKETVARTQNIGFYNTLFKDAGITPSDIKSLDDLAKIPFTKKADLRGGYPFGFLAVPM
NQVNRIHTTSGTTGKPTVVAYTKNDLNMWSELIARNLTMVGLRAGDIFQNASNYSLFTGGLGIHMGAEKIGCAVVPSGVG
NTKRQIEMIQDFKVKGLHCTPSYAMHLTEVAEEMHADLASLEIGCFGAEAWSENMRRDLENRLGLKAYDSYGMSELFGPG
VAFECPEQDGLHIWHDCYIVEIIDPKTGEVLGPEEKGEMVVTPIVKEAMPLLRYRTGDITMLMEDECPCGRGQKIARLLG
RSDDMLTVRGINVFPSQIEHVLKNIPEVGDQFIVYIDRVNHLDEMMIEVEMNKNIFSGELQDLSKLQTKIMKALQDTLTL
RARVELVEPGSLPRFEGKAKRVVDRRVI
>Mature_428_residues
MFWNEKMETLSGQALEELQLKRLKETVARTQNIGFYNTLFKDAGITPSDIKSLDDLAKIPFTKKADLRGGYPFGFLAVPM
NQVNRIHTTSGTTGKPTVVAYTKNDLNMWSELIARNLTMVGLRAGDIFQNASNYSLFTGGLGIHMGAEKIGCAVVPSGVG
NTKRQIEMIQDFKVKGLHCTPSYAMHLTEVAEEMHADLASLEIGCFGAEAWSENMRRDLENRLGLKAYDSYGMSELFGPG
VAFECPEQDGLHIWHDCYIVEIIDPKTGEVLGPEEKGEMVVTPIVKEAMPLLRYRTGDITMLMEDECPCGRGQKIARLLG
RSDDMLTVRGINVFPSQIEHVLKNIPEVGDQFIVYIDRVNHLDEMMIEVEMNKNIFSGELQDLSKLQTKIMKALQDTLTL
RARVELVEPGSLPRFEGKAKRVVDRRVI

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=429, Percent_Identity=42.1911421911422, Blast_Score=347, Evalue=1e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 47911; Mature: 47911

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFWNEKMETLSGQALEELQLKRLKETVARTQNIGFYNTLFKDAGITPSDIKSLDDLAKIP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHCC
FTKKADLRGGYPFGFLAVPMNQVNRIHTTSGTTGKPTVVAYTKNDLNMWSELIARNLTMV
CCCCCCCCCCCCCEEEEECHHHCCEEEECCCCCCCCEEEEEECCHHHHHHHHHHCCCEEE
GLRAGDIFQNASNYSLFTGGLGIHMGAEKIGCAVVPSGVGNTKRQIEMIQDFKVKGLHCT
EEECCHHHHCCCCCEEEECCCEEEECHHHHCCEEECCCCCCHHHHHHHHHHCCCCCEEEC
PSYAMHLTEVAEEMHADLASLEIGCFGAEAWSENMRRDLENRLGLKAYDSYGMSELFGPG
HHHHHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHCCCEECCCCCHHHHCCCC
VAFECPEQDGLHIWHDCYIVEIIDPKTGEVLGPEEKGEMVVTPIVKEAMPLLRYRTGDIT
CEEECCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCEE
MLMEDECPCGRGQKIARLLGRSDDMLTVRGINVFPSQIEHVLKNIPEVGDQFIVYIDRVN
EEEECCCCCCCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCHHCCCCEEEEEHHHC
HLDEMMIEVEMNKNIFSGELQDLSKLQTKIMKALQDTLTLRARVELVEPGSLPRFEGKAK
CHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHH
RVVDRRVI
HHHHHCCC
>Mature Secondary Structure
MFWNEKMETLSGQALEELQLKRLKETVARTQNIGFYNTLFKDAGITPSDIKSLDDLAKIP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHCC
FTKKADLRGGYPFGFLAVPMNQVNRIHTTSGTTGKPTVVAYTKNDLNMWSELIARNLTMV
CCCCCCCCCCCCCEEEEECHHHCCEEEECCCCCCCCEEEEEECCHHHHHHHHHHCCCEEE
GLRAGDIFQNASNYSLFTGGLGIHMGAEKIGCAVVPSGVGNTKRQIEMIQDFKVKGLHCT
EEECCHHHHCCCCCEEEECCCEEEECHHHHCCEEECCCCCCHHHHHHHHHHCCCCCEEEC
PSYAMHLTEVAEEMHADLASLEIGCFGAEAWSENMRRDLENRLGLKAYDSYGMSELFGPG
HHHHHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHCCCEECCCCCHHHHCCCC
VAFECPEQDGLHIWHDCYIVEIIDPKTGEVLGPEEKGEMVVTPIVKEAMPLLRYRTGDIT
CEEECCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCEE
MLMEDECPCGRGQKIARLLGRSDDMLTVRGINVFPSQIEHVLKNIPEVGDQFIVYIDRVN
EEEECCCCCCCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCHHCCCCEEEEEHHHC
HLDEMMIEVEMNKNIFSGELQDLSKLQTKIMKALQDTLTLRARVELVEPGSLPRFEGKAK
CHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHH
RVVDRRVI
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]