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The map label for this gene is paaK [H]

Identifier: 124486272

GI number: 124486272

Start: 1489569

End: 1490870

Strand: Reverse

Name: paaK [H]

Synonym: Mlab_1456

Alternate gene names: 124486272

Gene position: 1490870-1489569 (Counterclockwise)

Preceding gene: 124486273

Following gene: 124486271

Centisome position: 82.6

GC content: 52.23

Gene sequence:

>1302_bases
ATGGCGTATTATAACGAAAAGATCGAAACGATGCCCAAAGATGAGCTTTCGAAGCTCCAGTATCAGGAACTGAAAAAACT
GGTCACGCGTCTCTACGATTCATCGGCCTTTTACCATAAGAAGATGGACGAGACGGGTGTGAAACCCGCAGACATCACCT
CCATTACCGATATCACGAAGCTGCCTTTCATGAAAAAGACGGATCTCCGTGATAATTACCCGGACGGACTCGTCTGTGTC
CCGCACGAAGATCTGGTCAGGTACCATGTTTCATCCGGAACGACCGGCAAGCCGACGGTCGTTGCCTACACGCAGAATGA
TATCGATCTCTGGAGCGAGTGTGTTGCCCGCTCGCTTGTTTCGTGCGGGGTCGGAAGAAAGGACGTTTTACAGGTCTGCT
ACGGATACGGTCTTTTTACCGGCGGTCTCGGGCTGCATTACGGTGGAGAAAAAGTCGGTGCGACGGTGATCCCGGCATCC
ACCGGAAACTCCGAGCGGCAGATCGAGCTTATCCAGGATCTTAAAACGACGGCGATAGCCTGTACTCCTTCTTACTTCAT
CCATCTGATCGACGTTGCCAAAAAAATGGGTGTCGACTTCAGCAAAGACACGGTTCTTCGAACAGCAGTTCTCGGCGCCG
AGCCGTGGACGGATGCGATGCGCCGGTATATCTATGCCGAGTCCGGCGTTACGGCACATAACATCTTTGGTACGTCCGAG
ATTTCCGGCCCGATGTTTACGGACTGCAGCGAACTGAACGGTATGCATATCTGTGGAGATATCGCATACACCGAGATCAT
CGACCCGAAGACCGGCGAGCAGCTTCCCCCCGGAGAGAAGGGAGAACTGACTATCACGGTCCTGAAAAAGGAGGCGATCC
CGATGATCCGGTATCGGATCGGGGATATTACATCTATTATTGAAGGAGATTGTGCCTGCGGCAGAACTTCCCCGCGGATC
GACCGTCTGCAGGGCAGGGTCGACGATATGCTGATCATCCGCGGGATTAATGTCTTCCCGTCGGCTATCGAGCATGCCCT
CTTGAAAAACCCGTATCTCACGAGCCATTTCATGATCGAAGTGTTCAGAACGGGCCCTCTTGATGATATGCTCGTAAAGG
TAGAATTAAAGCCTGATGCGATGACAGATAGTATTAACGGTCTCATGGAGATGCAGATGCGTACCGAGCGTGCTCTTCGG
GATGCTTTGAATGTCTCTGCCAAGATCGAGCTCTGTCCGCCGAACTCCCTCCCGCGTTTTGAGGGTAAAGCAAAACGTGT
AATCGATCATCGGGTGATCTAA

Upstream 100 bases:

>100_bases
CGCTTACGCTGCGTGCCAGAGTCGAGCTTGTAGAGCCGGGGTCTCTCCCCCGCTTTGAAGGGAAAGCAAAGAGAGTCGTT
GACCGGAGGGTCATTTAAGT

Downstream 100 bases:

>100_bases
ATGACGGAAAAATACATCATCAAACAGTTGTCGATATTTTCAGAGAATAAGGCTGGAAAACTTGCCGCGATCGCGAAGAT
CTTTCTTGACACGGGCGTAT

Product: phenylacetate-CoA ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MAYYNEKIETMPKDELSKLQYQELKKLVTRLYDSSAFYHKKMDETGVKPADITSITDITKLPFMKKTDLRDNYPDGLVCV
PHEDLVRYHVSSGTTGKPTVVAYTQNDIDLWSECVARSLVSCGVGRKDVLQVCYGYGLFTGGLGLHYGGEKVGATVIPAS
TGNSERQIELIQDLKTTAIACTPSYFIHLIDVAKKMGVDFSKDTVLRTAVLGAEPWTDAMRRYIYAESGVTAHNIFGTSE
ISGPMFTDCSELNGMHICGDIAYTEIIDPKTGEQLPPGEKGELTITVLKKEAIPMIRYRIGDITSIIEGDCACGRTSPRI
DRLQGRVDDMLIIRGINVFPSAIEHALLKNPYLTSHFMIEVFRTGPLDDMLVKVELKPDAMTDSINGLMEMQMRTERALR
DALNVSAKIELCPPNSLPRFEGKAKRVIDHRVI

Sequences:

>Translated_433_residues
MAYYNEKIETMPKDELSKLQYQELKKLVTRLYDSSAFYHKKMDETGVKPADITSITDITKLPFMKKTDLRDNYPDGLVCV
PHEDLVRYHVSSGTTGKPTVVAYTQNDIDLWSECVARSLVSCGVGRKDVLQVCYGYGLFTGGLGLHYGGEKVGATVIPAS
TGNSERQIELIQDLKTTAIACTPSYFIHLIDVAKKMGVDFSKDTVLRTAVLGAEPWTDAMRRYIYAESGVTAHNIFGTSE
ISGPMFTDCSELNGMHICGDIAYTEIIDPKTGEQLPPGEKGELTITVLKKEAIPMIRYRIGDITSIIEGDCACGRTSPRI
DRLQGRVDDMLIIRGINVFPSAIEHALLKNPYLTSHFMIEVFRTGPLDDMLVKVELKPDAMTDSINGLMEMQMRTERALR
DALNVSAKIELCPPNSLPRFEGKAKRVIDHRVI
>Mature_432_residues
AYYNEKIETMPKDELSKLQYQELKKLVTRLYDSSAFYHKKMDETGVKPADITSITDITKLPFMKKTDLRDNYPDGLVCVP
HEDLVRYHVSSGTTGKPTVVAYTQNDIDLWSECVARSLVSCGVGRKDVLQVCYGYGLFTGGLGLHYGGEKVGATVIPAST
GNSERQIELIQDLKTTAIACTPSYFIHLIDVAKKMGVDFSKDTVLRTAVLGAEPWTDAMRRYIYAESGVTAHNIFGTSEI
SGPMFTDCSELNGMHICGDIAYTEIIDPKTGEQLPPGEKGELTITVLKKEAIPMIRYRIGDITSIIEGDCACGRTSPRID
RLQGRVDDMLIIRGINVFPSAIEHALLKNPYLTSHFMIEVFRTGPLDDMLVKVELKPDAMTDSINGLMEMQMRTERALRD
ALNVSAKIELCPPNSLPRFEGKAKRVIDHRVI

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=429, Percent_Identity=47.3193473193473, Blast_Score=372, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 48174; Mature: 48043

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYYNEKIETMPKDELSKLQYQELKKLVTRLYDSSAFYHKKMDETGVKPADITSITDITK
CCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHH
LPFMKKTDLRDNYPDGLVCVPHEDLVRYHVSSGTTGKPTVVAYTQNDIDLWSECVARSLV
CCCCCCCCCCCCCCCCEEECCCHHHHHEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHH
SCGVGRKDVLQVCYGYGLFTGGLGLHYGGEKVGATVIPASTGNSERQIELIQDLKTTAIA
HCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHEEE
CTPSYFIHLIDVAKKMGVDFSKDTVLRTAVLGAEPWTDAMRRYIYAESGVTAHNIFGTSE
ECHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCC
ISGPMFTDCSELNGMHICGDIAYTEIIDPKTGEQLPPGEKGELTITVLKKEAIPMIRYRI
CCCCCCCCHHHCCCEEEECCHHHHHEECCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHH
GDITSIIEGDCACGRTSPRIDRLQGRVDDMLIIRGINVFPSAIEHALLKNPYLTSHFMIE
HHHHHHHCCCCCCCCCCCHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCCHHHHHHH
VFRTGPLDDMLVKVELKPDAMTDSINGLMEMQMRTERALRDALNVSAKIELCPPNSLPRF
EECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCC
EGKAKRVIDHRVI
CCHHHHHHHHCCC
>Mature Secondary Structure 
AYYNEKIETMPKDELSKLQYQELKKLVTRLYDSSAFYHKKMDETGVKPADITSITDITK
CCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHH
LPFMKKTDLRDNYPDGLVCVPHEDLVRYHVSSGTTGKPTVVAYTQNDIDLWSECVARSLV
CCCCCCCCCCCCCCCCEEECCCHHHHHEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHH
SCGVGRKDVLQVCYGYGLFTGGLGLHYGGEKVGATVIPASTGNSERQIELIQDLKTTAIA
HCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHEEE
CTPSYFIHLIDVAKKMGVDFSKDTVLRTAVLGAEPWTDAMRRYIYAESGVTAHNIFGTSE
ECHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCC
ISGPMFTDCSELNGMHICGDIAYTEIIDPKTGEQLPPGEKGELTITVLKKEAIPMIRYRI
CCCCCCCCHHHCCCEEEECCHHHHHEECCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHH
GDITSIIEGDCACGRTSPRIDRLQGRVDDMLIIRGINVFPSAIEHALLKNPYLTSHFMIE
HHHHHHHCCCCCCCCCCCHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCCHHHHHHH
VFRTGPLDDMLVKVELKPDAMTDSINGLMEMQMRTERALRDALNVSAKIELCPPNSLPRF
EECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCC
EGKAKRVIDHRVI
CCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]