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The map label for this gene is hisF

Identifier: 124486225

GI number: 124486225

Start: 1439439

End: 1440245

Strand: Reverse

Name: hisF

Synonym: Mlab_1408

Alternate gene names: 124486225

Gene position: 1440245-1439439 (Counterclockwise)

Preceding gene: 124486226

Following gene: 124486224

Centisome position: 79.79

GC content: 53.66

Gene sequence:

>807_bases
ATGGGACTTACCCGGCGAATCATTCCCTGTCTTGATCTGAAAGCAGGAAGGGTAGTAAAGGGCGTAAATTTTGAAGGGCT
TCGCGATGCAGGTGATCCTGTCGAGCTCGCCTCACGATATAATGAACAGGGTGCCGATGAGGTCGTATTTCTGGATATTG
CGGCCTCGAAGGACAATCGCGATACGATGATCGACGTGATCGGCCGGGCTGCCGATGAGCTGTTTCTTCCGTTGACGGTC
GGCGGCGGGATTCGTACGGTCAAAGATATTCAGGATATTCTGCGTGCCGGCGCCGATAAGGTAAGTCTGAACACGTCTGC
GGTAAAAACTCCTGACGTAATCAACGAAGGATCACATCTGTTCGGGAATCAATGTATCGTTCTTGCCGAAGATGTCCGGA
GAAATTATGAGATGCGGGACGGCGTGACCCCGATCCATCTCGCAGACGGGAGAGTCTGCTGGTATGAAGTGGTCATCTAC
GGTGGCAGCCAGCGGACCGGCATCGATGCGGTCTCCTGGGCGCAGGAAGGTGTCAAAAGAGGTGCCGGCGAGATCCTTCT
CACATCGATGGAAACAGACGGCGTCAAGACCGGTTTCGATTTGGAGATCACCGCAGCCATCTCGGAAAATGTCGATGTTC
CGGTGATCGCATCCGGTGGGGTCGGGACCCTTGAACATTTTTACGACGGCTTTGTGAAAGGAAAAGCTGATGCATGCCTT
GCGGCAAGTGTCTTCCATTACGGAGAGATGACGATCCGATCCGTGAAGGAGTATTTGGCTTCCCGGGGCGTTTTGGTCCG
GTTGTAA

Upstream 100 bases:

>100_bases
ATACAATTGCCAGAATGGATGATCTGAAAGCGTTTCTGGAAAAGTAATCCATTCTCCTTTTTCAAACAGGCTTTTCTCCA
AAAAACACCAATCATATCCT

Downstream 100 bases:

>100_bases
TGGGGTGGCAGACCGATTATCAGGCACGCGGGTCTCTGTGGGCAGGGGCATCCTATCTCCTGCCCGAGATCCCGGAAAAT
GCTCTTATCCTTGAGACCGG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MGLTRRIIPCLDLKAGRVVKGVNFEGLRDAGDPVELASRYNEQGADEVVFLDIAASKDNRDTMIDVIGRAADELFLPLTV
GGGIRTVKDIQDILRAGADKVSLNTSAVKTPDVINEGSHLFGNQCIVLAEDVRRNYEMRDGVTPIHLADGRVCWYEVVIY
GGSQRTGIDAVSWAQEGVKRGAGEILLTSMETDGVKTGFDLEITAAISENVDVPVIASGGVGTLEHFYDGFVKGKADACL
AASVFHYGEMTIRSVKEYLASRGVLVRL

Sequences:

>Translated_268_residues
MGLTRRIIPCLDLKAGRVVKGVNFEGLRDAGDPVELASRYNEQGADEVVFLDIAASKDNRDTMIDVIGRAADELFLPLTV
GGGIRTVKDIQDILRAGADKVSLNTSAVKTPDVINEGSHLFGNQCIVLAEDVRRNYEMRDGVTPIHLADGRVCWYEVVIY
GGSQRTGIDAVSWAQEGVKRGAGEILLTSMETDGVKTGFDLEITAAISENVDVPVIASGGVGTLEHFYDGFVKGKADACL
AASVFHYGEMTIRSVKEYLASRGVLVRL
>Mature_267_residues
GLTRRIIPCLDLKAGRVVKGVNFEGLRDAGDPVELASRYNEQGADEVVFLDIAASKDNRDTMIDVIGRAADELFLPLTVG
GGIRTVKDIQDILRAGADKVSLNTSAVKTPDVINEGSHLFGNQCIVLAEDVRRNYEMRDGVTPIHLADGRVCWYEVVIYG
GSQRTGIDAVSWAQEGVKRGAGEILLTSMETDGVKTGFDLEITAAISENVDVPVIASGGVGTLEHFYDGFVKGKADACLA
ASVFHYGEMTIRSVKEYLASRGVLVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=270, Percent_Identity=43.3333333333333, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI87082028, Length=223, Percent_Identity=26.9058295964126, Blast_Score=75, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319725, Length=317, Percent_Identity=38.801261829653, Blast_Score=191, Evalue=8e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_METLZ (A2STB8)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001030841.1
- ProteinModelPortal:   A2STB8
- SMR:   A2STB8
- STRING:   A2STB8
- GeneID:   4796194
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_1408
- eggNOG:   arNOG04606
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   A2STB8
- ProtClustDB:   PRK02083
- BioCyc:   MLAB410358:MLAB_1408-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 28877; Mature: 28746

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: ACT_SITE 12-12 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLTRRIIPCLDLKAGRVVKGVNFEGLRDAGDPVELASRYNEQGADEVVFLDIAASKDNR
CCCCCCCCHHCCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCC
DTMIDVIGRAADELFLPLTVGGGIRTVKDIQDILRAGADKVSLNTSAVKTPDVINEGSHL
HHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHCCCCCC
FGNQCIVLAEDVRRNYEMRDGVTPIHLADGRVCWYEVVIYGGSQRTGIDAVSWAQEGVKR
CCCEEEEEEHHHHCCCHHHCCCCEEEECCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHC
GAGEILLTSMETDGVKTGFDLEITAAISENVDVPVIASGGVGTLEHFYDGFVKGKADACL
CCCEEEEEEECCCCCCCCCCEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHH
AASVFHYGEMTIRSVKEYLASRGVLVRL
HHHHHHHHHHHHHHHHHHHHCCCEEEEC
>Mature Secondary Structure 
GLTRRIIPCLDLKAGRVVKGVNFEGLRDAGDPVELASRYNEQGADEVVFLDIAASKDNR
CCCCCCCHHCCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCC
DTMIDVIGRAADELFLPLTVGGGIRTVKDIQDILRAGADKVSLNTSAVKTPDVINEGSHL
HHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHCCCCCC
FGNQCIVLAEDVRRNYEMRDGVTPIHLADGRVCWYEVVIYGGSQRTGIDAVSWAQEGVKR
CCCEEEEEEHHHHCCCHHHCCCCEEEECCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHC
GAGEILLTSMETDGVKTGFDLEITAAISENVDVPVIASGGVGTLEHFYDGFVKGKADACL
CCCEEEEEEECCCCCCCCCCEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHH
AASVFHYGEMTIRSVKEYLASRGVLVRL
HHHHHHHHHHHHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA