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The map label for this gene is eif2a [H]

Identifier: 124486118

GI number: 124486118

Start: 1325588

End: 1326361

Strand: Reverse

Name: eif2a [H]

Synonym: Mlab_1299

Alternate gene names: 124486118

Gene position: 1326361-1325588 (Counterclockwise)

Preceding gene: 124486119

Following gene: 298205347

Centisome position: 73.48

GC content: 50.0

Gene sequence:

>774_bases
ATGAGTGAAAGAGATTGGCCAATAGAAGGAGAACTTGTCGTCTGCAGCGTTACAGAAGTCAAGGACTTCGCGGCATTTGT
AAACCTGGATGAATATGAAGGACGTCAGGGGTTAATTCCAATCGCGGAAATTGCCCGTGGCTGGATTAAATATATCCGGG
ACTACATCCGTGAAGGACAGAAAGTTGTCTGTAAAGTCCTTCACGTCGATGAACACCGCGGCCATATCGATCTTTCCCTC
AAAGATGTCAATGAACACCAGCGCCGGGAAAAGATCCAGGACTGGAAAAACGAGCAGAAGGCACACAAGTGGATAGGGTT
TGCCTCAGCCGAGTCGAAGGTCGACGTCAAGACGTTTGAAGAGGCAATGTATGCCGAATTTGGTTCGCTTTATGCCGCGT
TCGAGGGTATCGCTTTATATGGCGATGCAACTCTCGCCAAATTCTCGCTTCCAGCCGAAGCAGCAGCTGCCTTGGCAAAG
GTTGCTTCTGAAAATGTCAAGGTTCCAAAAGTCACCGTCAGTGCGATTTTGGAACTGACCTCCACCAAACCCGACGGAGT
CAACATTATTCGCCGCGCTCTTAGGAGTGCTGAACCCAAAGTTGACGGTGCCGAGATCGAACTTCTCTATCTTGGAGCGC
CGCACTACCGGGTTAAAGTTGTCGCCCCGGATTATAAGACTGCAGAAAAAGCTCTGGTCAAAGCATCCGAAGCAGCGATC
GGTGTGATGGAACGCGCTGAAGGTTCCGGCAAACTCATCCGGAAACAGAAGTAA

Upstream 100 bases:

>100_bases
GCACAGTCTGCGGCCGCATCCTTGCAGAGCCGACCGGCGGCAAAGCTGCTCTTAAAGCAGATATCGTAGCAACATTTGAA
TAAATCTGGAAAATTATCTA

Downstream 100 bases:

>100_bases
TATCCGGTGTTTTTCATGACAGGTCATATTCGTAGATGCCCTGAATGCAACACCTACACTCTTTTTAATACATGTCAGAA
ATGCGGATGTCCTACGGTGT

Product: translation initiation factor IF-2 subunit alpha

Products: RNAn; a nucleoside diphosphate [C]

Alternate protein names: aIF2-alpha; eIF-2-alpha [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MSERDWPIEGELVVCSVTEVKDFAAFVNLDEYEGRQGLIPIAEIARGWIKYIRDYIREGQKVVCKVLHVDEHRGHIDLSL
KDVNEHQRREKIQDWKNEQKAHKWIGFASAESKVDVKTFEEAMYAEFGSLYAAFEGIALYGDATLAKFSLPAEAAAALAK
VASENVKVPKVTVSAILELTSTKPDGVNIIRRALRSAEPKVDGAEIELLYLGAPHYRVKVVAPDYKTAEKALVKASEAAI
GVMERAEGSGKLIRKQK

Sequences:

>Translated_257_residues
MSERDWPIEGELVVCSVTEVKDFAAFVNLDEYEGRQGLIPIAEIARGWIKYIRDYIREGQKVVCKVLHVDEHRGHIDLSL
KDVNEHQRREKIQDWKNEQKAHKWIGFASAESKVDVKTFEEAMYAEFGSLYAAFEGIALYGDATLAKFSLPAEAAAALAK
VASENVKVPKVTVSAILELTSTKPDGVNIIRRALRSAEPKVDGAEIELLYLGAPHYRVKVVAPDYKTAEKALVKASEAAI
GVMERAEGSGKLIRKQK
>Mature_256_residues
SERDWPIEGELVVCSVTEVKDFAAFVNLDEYEGRQGLIPIAEIARGWIKYIRDYIREGQKVVCKVLHVDEHRGHIDLSLK
DVNEHQRREKIQDWKNEQKAHKWIGFASAESKVDVKTFEEAMYAEFGSLYAAFEGIALYGDATLAKFSLPAEAAAALAKV
ASENVKVPKVTVSAILELTSTKPDGVNIIRRALRSAEPKVDGAEIELLYLGAPHYRVKVVAPDYKTAEKALVKASEAAIG
VMERAEGSGKLIRKQK

Specific function: eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA [H]

COG id: COG1093

COG function: function code J; Translation initiation factor 2, alpha subunit (eIF-2alpha)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17509871, Length=235, Percent_Identity=24.6808510638298, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322466, Length=252, Percent_Identity=23.8095238095238, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011488
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR022964 [H]

Pfam domain/function: PF07541 EIF_2_alpha; PF00575 S1 [H]

EC number: 2.7.7.8 [C]

Molecular weight: Translated: 28597; Mature: 28466

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERDWPIEGELVVCSVTEVKDFAAFVNLDEYEGRQGLIPIAEIARGWIKYIRDYIREGQ
CCCCCCCCCCCEEEEEHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCH
KVVCKVLHVDEHRGHIDLSLKDVNEHQRREKIQDWKNEQKAHKWIGFASAESKVDVKTFE
HHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHH
EAMYAEFGSLYAAFEGIALYGDATLAKFSLPAEAAAALAKVASENVKVPKVTVSAILELT
HHHHHHHHHHHHHHCCEEEECCCHHEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
STKPDGVNIIRRALRSAEPKVDGAEIELLYLGAPHYRVKVVAPDYKTAEKALVKASEAAI
CCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHH
GVMERAEGSGKLIRKQK
HHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SERDWPIEGELVVCSVTEVKDFAAFVNLDEYEGRQGLIPIAEIARGWIKYIRDYIREGQ
CCCCCCCCCCEEEEEHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCH
KVVCKVLHVDEHRGHIDLSLKDVNEHQRREKIQDWKNEQKAHKWIGFASAESKVDVKTFE
HHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHH
EAMYAEFGSLYAAFEGIALYGDATLAKFSLPAEAAAALAKVASENVKVPKVTVSAILELT
HHHHHHHHHHHHHHCCEEEECCCHHEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
STKPDGVNIIRRALRSAEPKVDGAEIELLYLGAPHYRVKVVAPDYKTAEKALVKASEAAI
CCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHH
GVMERAEGSGKLIRKQK
HHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 4500 [C]

Specific activity: NA

Km value (mM): NA

Substrates: RNAn+1; phosphate [C]

Specific reaction: RNAn+1 + phosphate = RNAn + a nucleoside diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA