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The map label for this gene is radB [H]

Identifier: 124486092

GI number: 124486092

Start: 1299628

End: 1300302

Strand: Reverse

Name: radB [H]

Synonym: Mlab_1272

Alternate gene names: 124486092

Gene position: 1300302-1299628 (Counterclockwise)

Preceding gene: 124486093

Following gene: 124486088

Centisome position: 72.04

GC content: 53.33

Gene sequence:

>675_bases
ATGGAAGACGCCGTAAAAAAAATATCAACCGGAGCCTCTGGACTTGACACCCTTCTCGGCGGCGGCATTGAGCCGCGTAT
GATCACCCAGTTTTTTGGAGAGGCCGGTTCGGGAAAGAGTACGCTTTGTATGACGGCAGCCGTTTCGGTGCTTCGAAAAG
GTCTGGGGGTCGTCTATATCGATTCGGAAGGTTTCTCCATCGAGCGGTTTTCGCAGATAGCCGGAGCCTCTACCGAAGAG
TGCGCCAAGCATCTGTATGTATTTGAGCCGGCGACCTTTGCCGAACAGGGTCTGATGATTTCAGAGTCGGAACAGCTGAT
CAAATCCGGGACTGCCGGACTGATCGTGCTTGACTCGGCAACTGCCCTCTACCGTGTGGAACAGATGGATACGAAAGACG
CTCTTTCGATGCTTTCACGCCAGATGATGGTGCTTCTGGGAATTGCCAAGAGATATGATATTCCCGTTCTCATCACCAAT
CAGGTGTTCATGGACATCGATCATCACCGACTGAGCGGTCTTGGAGGAACTGCGTTGTCGCATATCTCAAAAGCGATCAT
CCGTGTGGAAAAACACGAGGGATTCAGACGGGCCGTCGTTTCCAAACACAGGTCCCGCCCTGAGGGTATGTCCTGGGAGT
TTGTTCTCACCGGTACCGGGGTCAGGGACCGCTGA

Upstream 100 bases:

>100_bases
TGGTCTATTCCCGCAAAGCCGAATTCGATGATCTTCAGCGAATCTCGGATGCATCCGGTCTTCCGGTTCGAACGGTAAAA
CGCATCGTCGAGGAAGATGT

Downstream 100 bases:

>100_bases
CCTCCGCCCTTTTTTTGATAAAAAAAGTATTGCTGTTTTACTCTTGGGATTCTGCATCGAGTACAGCACAGACGTTTTCG
GTCGTATCTCTGATCAGTTT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MEDAVKKISTGASGLDTLLGGGIEPRMITQFFGEAGSGKSTLCMTAAVSVLRKGLGVVYIDSEGFSIERFSQIAGASTEE
CAKHLYVFEPATFAEQGLMISESEQLIKSGTAGLIVLDSATALYRVEQMDTKDALSMLSRQMMVLLGIAKRYDIPVLITN
QVFMDIDHHRLSGLGGTALSHISKAIIRVEKHEGFRRAVVSKHRSRPEGMSWEFVLTGTGVRDR

Sequences:

>Translated_224_residues
MEDAVKKISTGASGLDTLLGGGIEPRMITQFFGEAGSGKSTLCMTAAVSVLRKGLGVVYIDSEGFSIERFSQIAGASTEE
CAKHLYVFEPATFAEQGLMISESEQLIKSGTAGLIVLDSATALYRVEQMDTKDALSMLSRQMMVLLGIAKRYDIPVLITN
QVFMDIDHHRLSGLGGTALSHISKAIIRVEKHEGFRRAVVSKHRSRPEGMSWEFVLTGTGVRDR
>Mature_224_residues
MEDAVKKISTGASGLDTLLGGGIEPRMITQFFGEAGSGKSTLCMTAAVSVLRKGLGVVYIDSEGFSIERFSQIAGASTEE
CAKHLYVFEPATFAEQGLMISESEQLIKSGTAGLIVLDSATALYRVEQMDTKDALSMLSRQMMVLLGIAKRYDIPVLITN
QVFMDIDHHRLSGLGGTALSHISKAIIRVEKHEGFRRAVVSKHRSRPEGMSWEFVLTGTGVRDR

Specific function: Involved in DNA repair and in homologous recombination. May regulate the cleavage reactions of the branch-structured DNA. Has a very weak ATPase activity that is not stimulated by DNA. Binds DNA but does not promote DNA strands exchange [H]

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic recA-like protein family. RadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI19924133, Length=238, Percent_Identity=30.2521008403361, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI23238219, Length=250, Percent_Identity=28, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI256017143, Length=224, Percent_Identity=29.0178571428571, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI256017141, Length=224, Percent_Identity=29.0178571428571, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI19924117, Length=177, Percent_Identity=31.638418079096, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI10835029, Length=177, Percent_Identity=31.638418079096, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI46255039, Length=177, Percent_Identity=31.638418079096, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI256017145, Length=131, Percent_Identity=34.3511450381679, Blast_Score=64, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI71997295, Length=238, Percent_Identity=29.4117647058824, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71997301, Length=238, Percent_Identity=29.4117647058824, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6321027, Length=241, Percent_Identity=30.2904564315353, Blast_Score=78, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320942, Length=130, Percent_Identity=36.9230769230769, Blast_Score=66, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24651285, Length=218, Percent_Identity=30.2752293577982, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17864108, Length=218, Percent_Identity=30.2752293577982, Blast_Score=71, Evalue=6e-13,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013632
- InterPro:   IPR011939
- InterPro:   IPR020588 [H]

Pfam domain/function: PF08423 Rad51 [H]

EC number: NA

Molecular weight: Translated: 24344; Mature: 24344

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDAVKKISTGASGLDTLLGGGIEPRMITQFFGEAGSGKSTLCMTAAVSVLRKGLGVVYI
CCHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEE
DSEGFSIERFSQIAGASTEECAKHLYVFEPATFAEQGLMISESEQLIKSGTAGLIVLDSA
CCCCCCHHHHHHHHCCCHHHHHHHEEEECCCHHHHCCCEECCHHHHHHCCCCCEEEEECC
TALYRVEQMDTKDALSMLSRQMMVLLGIAKRYDIPVLITNQVFMDIDHHRLSGLGGTALS
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEECCHHHHCCCCHHHHH
HISKAIIRVEKHEGFRRAVVSKHRSRPEGMSWEFVLTGTGVRDR
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MEDAVKKISTGASGLDTLLGGGIEPRMITQFFGEAGSGKSTLCMTAAVSVLRKGLGVVYI
CCHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEE
DSEGFSIERFSQIAGASTEECAKHLYVFEPATFAEQGLMISESEQLIKSGTAGLIVLDSA
CCCCCCHHHHHHHHCCCHHHHHHHEEEECCCHHHHCCCEECCHHHHHHCCCCCEEEEECC
TALYRVEQMDTKDALSMLSRQMMVLLGIAKRYDIPVLITNQVFMDIDHHRLSGLGGTALS
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEECCHHHHCCCCHHHHH
HISKAIIRVEKHEGFRRAVVSKHRSRPEGMSWEFVLTGTGVRDR
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA