Click here to switch to the map view.

The map label for this gene is pgk

Identifier: 124486071

GI number: 124486071

Start: 1277077

End: 1278294

Strand: Reverse

Name: pgk

Synonym: Mlab_1251

Alternate gene names: 124486071

Gene position: 1278294-1277077 (Counterclockwise)

Preceding gene: 124486072

Following gene: 124486069

Centisome position: 70.82

GC content: 53.45

Gene sequence:

>1218_bases
ATGAATTTTGGGACTCTTGCAGATATTGAAACACGCGGAAAAACCGTTCTCGTCCGTGTGGATTTCAACTCCCCTATAGA
TCCTTCCTCCAATACTATTTTGGACGACAAACGGTTCCGCGAACACATCCCAACGCTGCAGGCACTGGAAGATGCCCGCG
TCGTGGTCCTTGCCCACCAGAGCAGACCCGGGAAAAAAGATTTCACTACGCTTGCCGCCCACGCCGATAAACTGGAGCGG
CTCATGAATATGCCGGTCACCTATGTGGATGATATCTTCGGCACCTGCGCAAAGCATGCAGTCCGCGATGCTCATCCGGG
AGATATCGTTCTTCTGGAAAACGTCAGGTTCTCAGCTGAAGAGAATCTGACCATGAAGCCGGAGGATGGAGCAAAGACCC
ATCTTGTTAGAAAACTTGCCTCCATGGCGGATCTTTTTGTATATGATGCGTTTGGCACGGCCCACCGATCCCAGCCGACG
ATCACCGGTCTTTCCTATGCCATGAAAACGGTTGCCGGCCTTCTCATGGAAAAAGAAGTGTCCATGCTTTCAAGAGTCTT
CACCTCGGCACCCAAACCCGTGACCTTCGTTCTGGGCGGAACGAAGGTGGATGATTCCATCGCGGTTGCCGGAAACGTTC
TCGCAAACAAGACCGCCGACAAAGTTGCCATTATCGGTGTGGTTGCAAACGTCTTTTATCTGGCAAAAGGGATCGACATC
GGGGAGCCTTCACGCAACCTGATCCATATGCTCGGCTATGACGATGAAGTAGCGAAGGCAAAAACCATTCTCGACTCCTA
TCCCGATAAGGTCCTTCTGCCGGAATATGTCGCGGTAAAAGAGCATGATGAGCGTAAGGAGTATCCGATCACCCGTGTTC
CCAAAGACTGCCCGATCCTCGATATGGGTACGGAGTCTATTGTGCAGTTCTCAAAAGCACTGAGGAACTCGGGTTCGATC
GTGTTCAACGGCCCGGCAGGGGTATTCGAAGAGGCAAAGTTTGCCTCGGGTACGTTTGAACTCCTGCGGGCGGCGGCGAA
CGTGGACTTTTCCGTATGCGGCGGCGGCCACACGGCGGCGGTCATCGAACAGCTGGGTCTTGAACCGCAGTACTCCCATC
TTTCCACCGGCGGCGGGGCCTGCATCGAGTTTTTGACCGGAAAGAAACTGCCGGCGATCGCAGCTCTCGAAGAGTCCTGG
AAAAAATTCGGGAACTGA

Upstream 100 bases:

>100_bases
AAGAAAGCGGAGACGTCAGCTGAAGAAAATCCCGATGCCGGGTCTCCCGGGCCGTGAAGACACTTGGTATTTATAGGTAC
AAAAAGCCAATCTTTTTGAT

Downstream 100 bases:

>100_bases
TCCACTTCTTTTTTGGAATAGAACTTACGCGGTGTTGTGATCTTGGCCGAGAAAAAATTGACGTACTTATGAGGTATACG
TCGATTTCGTGTACGGCATT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MNFGTLADIETRGKTVLVRVDFNSPIDPSSNTILDDKRFREHIPTLQALEDARVVVLAHQSRPGKKDFTTLAAHADKLER
LMNMPVTYVDDIFGTCAKHAVRDAHPGDIVLLENVRFSAEENLTMKPEDGAKTHLVRKLASMADLFVYDAFGTAHRSQPT
ITGLSYAMKTVAGLLMEKEVSMLSRVFTSAPKPVTFVLGGTKVDDSIAVAGNVLANKTADKVAIIGVVANVFYLAKGIDI
GEPSRNLIHMLGYDDEVAKAKTILDSYPDKVLLPEYVAVKEHDERKEYPITRVPKDCPILDMGTESIVQFSKALRNSGSI
VFNGPAGVFEEAKFASGTFELLRAAANVDFSVCGGGHTAAVIEQLGLEPQYSHLSTGGGACIEFLTGKKLPAIAALEESW
KKFGN

Sequences:

>Translated_405_residues
MNFGTLADIETRGKTVLVRVDFNSPIDPSSNTILDDKRFREHIPTLQALEDARVVVLAHQSRPGKKDFTTLAAHADKLER
LMNMPVTYVDDIFGTCAKHAVRDAHPGDIVLLENVRFSAEENLTMKPEDGAKTHLVRKLASMADLFVYDAFGTAHRSQPT
ITGLSYAMKTVAGLLMEKEVSMLSRVFTSAPKPVTFVLGGTKVDDSIAVAGNVLANKTADKVAIIGVVANVFYLAKGIDI
GEPSRNLIHMLGYDDEVAKAKTILDSYPDKVLLPEYVAVKEHDERKEYPITRVPKDCPILDMGTESIVQFSKALRNSGSI
VFNGPAGVFEEAKFASGTFELLRAAANVDFSVCGGGHTAAVIEQLGLEPQYSHLSTGGGACIEFLTGKKLPAIAALEESW
KKFGN
>Mature_405_residues
MNFGTLADIETRGKTVLVRVDFNSPIDPSSNTILDDKRFREHIPTLQALEDARVVVLAHQSRPGKKDFTTLAAHADKLER
LMNMPVTYVDDIFGTCAKHAVRDAHPGDIVLLENVRFSAEENLTMKPEDGAKTHLVRKLASMADLFVYDAFGTAHRSQPT
ITGLSYAMKTVAGLLMEKEVSMLSRVFTSAPKPVTFVLGGTKVDDSIAVAGNVLANKTADKVAIIGVVANVFYLAKGIDI
GEPSRNLIHMLGYDDEVAKAKTILDSYPDKVLLPEYVAVKEHDERKEYPITRVPKDCPILDMGTESIVQFSKALRNSGSI
VFNGPAGVFEEAKFASGTFELLRAAANVDFSVCGGGHTAAVIEQLGLEPQYSHLSTGGGACIEFLTGKKLPAIAALEESW
KKFGN

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=419, Percent_Identity=31.980906921241, Blast_Score=185, Evalue=8e-47,
Organism=Homo sapiens, GI31543397, Length=419, Percent_Identity=31.2649164677804, Blast_Score=175, Evalue=6e-44,
Organism=Escherichia coli, GI1789294, Length=413, Percent_Identity=33.8983050847458, Blast_Score=192, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17508823, Length=406, Percent_Identity=33.4975369458128, Blast_Score=197, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6319857, Length=416, Percent_Identity=32.6923076923077, Blast_Score=186, Evalue=6e-48,
Organism=Drosophila melanogaster, GI24581203, Length=421, Percent_Identity=34.6793349168646, Blast_Score=194, Evalue=7e-50,
Organism=Drosophila melanogaster, GI17136394, Length=415, Percent_Identity=31.8072289156626, Blast_Score=172, Evalue=3e-43,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_METLZ (A2SSW4)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001030687.1
- ProteinModelPortal:   A2SSW4
- SMR:   A2SSW4
- STRING:   A2SSW4
- GeneID:   4794834
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_1251
- eggNOG:   arNOG04384
- HOGENOM:   HBG453500
- OMA:   PGKKDFT
- ProtClustDB:   PRK00073
- BioCyc:   MLAB410358:MLAB_1251-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 43954; Mature: 43954

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 38-38 BINDING 116-116 BINDING 156-156 BINDING 330-330

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFGTLADIETRGKTVLVRVDFNSPIDPSSNTILDDKRFREHIPTLQALEDARVVVLAHQ
CCCCCCCEEECCCCEEEEEEECCCCCCCCCCCEECHHHHHHHCCHHHHHCCCEEEEEEEC
SRPGKKDFTTLAAHADKLERLMNMPVTYVDDIFGTCAKHAVRDAHPGDIVLLENVRFSAE
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEECCC
ENLTMKPEDGAKTHLVRKLASMADLFVYDAFGTAHRSQPTITGLSYAMKTVAGLLMEKEV
CCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SMLSRVFTSAPKPVTFVLGGTKVDDSIAVAGNVLANKTADKVAIIGVVANVFYLAKGIDI
HHHHHHHHCCCCCEEEEECCCCCCCCHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
GEPSRNLIHMLGYDDEVAKAKTILDSYPDKVLLPEYVAVKEHDERKEYPITRVPKDCPIL
CCCCHHHEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCHHCCCCCCCCCCCCEE
DMGTESIVQFSKALRNSGSIVFNGPAGVFEEAKFASGTFELLRAAANVDFSVCGGGHTAA
ECCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHCCHHHHHHHHHHCCCEEEECCCHHHH
VIEQLGLEPQYSHLSTGGGACIEFLTGKKLPAIAALEESWKKFGN
HHHHHCCCCCHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFGTLADIETRGKTVLVRVDFNSPIDPSSNTILDDKRFREHIPTLQALEDARVVVLAHQ
CCCCCCCEEECCCCEEEEEEECCCCCCCCCCCEECHHHHHHHCCHHHHHCCCEEEEEEEC
SRPGKKDFTTLAAHADKLERLMNMPVTYVDDIFGTCAKHAVRDAHPGDIVLLENVRFSAE
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEECCC
ENLTMKPEDGAKTHLVRKLASMADLFVYDAFGTAHRSQPTITGLSYAMKTVAGLLMEKEV
CCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SMLSRVFTSAPKPVTFVLGGTKVDDSIAVAGNVLANKTADKVAIIGVVANVFYLAKGIDI
HHHHHHHHCCCCCEEEEECCCCCCCCHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
GEPSRNLIHMLGYDDEVAKAKTILDSYPDKVLLPEYVAVKEHDERKEYPITRVPKDCPIL
CCCCHHHEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCHHCCCCCCCCCCCCEE
DMGTESIVQFSKALRNSGSIVFNGPAGVFEEAKFASGTFELLRAAANVDFSVCGGGHTAA
ECCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHCCHHHHHHHHHHCCCEEEECCCHHHH
VIEQLGLEPQYSHLSTGGGACIEFLTGKKLPAIAALEESWKKFGN
HHHHHCCCCCHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA