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The map label for this gene is priB [H]

Identifier: 124486072

GI number: 124486072

Start: 1278338

End: 1279402

Strand: Reverse

Name: priB [H]

Synonym: Mlab_1252

Alternate gene names: 124486072

Gene position: 1279402-1278338 (Counterclockwise)

Preceding gene: 124486073

Following gene: 124486071

Centisome position: 70.88

GC content: 55.59

Gene sequence:

>1065_bases
ATGCGCACGTCCGTTGAAAAGCGCGACCTGGCTCATTATCCTTTTTTACCTGAAGCACAGGGGCTGCTTGCATCCCGCGG
AATCAAAGTTGCCGGGCTTTCCGGCTCGAAGATCGGCTGTTCCTATCTCGACCGGGCTGCCGAACGGGTGATTACGGCAC
TGGATGGAAAAACCGTTTACCCCGAGTCGGAAGACGATGTTGTCTCCGACATCATAACCTATGTTCTTGCCAGAGTGCTT
GCCTCCGCCTCGAAAGACAAGCGGACAATGGACCGGCTCTGCAGGATGGAGGCATCCCGCGTCTATGCGTATTTCCATGC
CGAAGAGAACGAGCTTTTGAAAAAGCGGGTCTGCGAGGAACTGGGTCTTGAACTGGGCGACGGTGCTCTTCCGGTGATCC
GGTATGTAGAGCTTGCCTCCCGCGTGCGTGATGCGAAGTGGCGGCTGATCAACCGGGACGTGGAAAACGGGTTTGTCTCC
GTCTCTTCCGACGAGATGGATGTTTTGATCGCTGAACGAATACGGGCGATCCTTTCCTCCGGTCTGCCGCTCGACATCCC
GGAATCGATCGCAAAGGAGTATCTTCCCTGGAGCGACAAGATCCTTTCTGCCGTGCAGGAGAGAACGCTTGTTGAGTTCG
GGGCGATCGACGAATCGGCGTATCCTCCCTGTATTCAGGCTTTGATCTCCGCGGCGGCGGCAGGCGTGAATCTCACGCAT
TCGGGCCGTTTTTCCATGACGGCATTTCTGCACAATGTCGGGATGAACGCCGAGCAGGTTGCGGGAATTTTTGCACGAAG
CCCGGATTACAATCCCGATATGACGAACTATCAGGTCGATCATATCCTGACGAACGAGTACACGACCCCTTCCTGCGCCA
CCATGCTGACCCACGGGGTCTGCGTAAATAAGGACCGGCTCTGCGAGTCGGTTTCGCATCCGCTGAATTACTACCGTTCC
AAAAAGAAGATGCTGGAAAAAATGCGGATCAAAGACGAAGCTCTGGCAAAGAAAGCGGAGACGTCAGCTGAAGAAAATCC
CGATGCCGGGTCTCCCGGGCCGTGA

Upstream 100 bases:

>100_bases
TGGGCACCGATCACCCGGTCCAGTTCTCCTTCGTCTATGCAGGCGGCAAGGGAAGTGTGGGCTACCTTCTTGCGCCGAGG
ATCGAGGCAGATTAACCGTT

Downstream 100 bases:

>100_bases
AGACACTTGGTATTTATAGGTACAAAAAGCCAATCTTTTTGATATGAATTTTGGGACTCTTGCAGATATTGAAACACGCG
GAAAAACCGTTCTCGTCCGT

Product: DNA primase large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MRTSVEKRDLAHYPFLPEAQGLLASRGIKVAGLSGSKIGCSYLDRAAERVITALDGKTVYPESEDDVVSDIITYVLARVL
ASASKDKRTMDRLCRMEASRVYAYFHAEENELLKKRVCEELGLELGDGALPVIRYVELASRVRDAKWRLINRDVENGFVS
VSSDEMDVLIAERIRAILSSGLPLDIPESIAKEYLPWSDKILSAVQERTLVEFGAIDESAYPPCIQALISAAAAGVNLTH
SGRFSMTAFLHNVGMNAEQVAGIFARSPDYNPDMTNYQVDHILTNEYTTPSCATMLTHGVCVNKDRLCESVSHPLNYYRS
KKKMLEKMRIKDEALAKKAETSAEENPDAGSPGP

Sequences:

>Translated_354_residues
MRTSVEKRDLAHYPFLPEAQGLLASRGIKVAGLSGSKIGCSYLDRAAERVITALDGKTVYPESEDDVVSDIITYVLARVL
ASASKDKRTMDRLCRMEASRVYAYFHAEENELLKKRVCEELGLELGDGALPVIRYVELASRVRDAKWRLINRDVENGFVS
VSSDEMDVLIAERIRAILSSGLPLDIPESIAKEYLPWSDKILSAVQERTLVEFGAIDESAYPPCIQALISAAAAGVNLTH
SGRFSMTAFLHNVGMNAEQVAGIFARSPDYNPDMTNYQVDHILTNEYTTPSCATMLTHGVCVNKDRLCESVSHPLNYYRS
KKKMLEKMRIKDEALAKKAETSAEENPDAGSPGP
>Mature_354_residues
MRTSVEKRDLAHYPFLPEAQGLLASRGIKVAGLSGSKIGCSYLDRAAERVITALDGKTVYPESEDDVVSDIITYVLARVL
ASASKDKRTMDRLCRMEASRVYAYFHAEENELLKKRVCEELGLELGDGALPVIRYVELASRVRDAKWRLINRDVENGFVS
VSSDEMDVLIAERIRAILSSGLPLDIPESIAKEYLPWSDKILSAVQERTLVEFGAIDESAYPPCIQALISAAAAGVNLTH
SGRFSMTAFLHNVGMNAEQVAGIFARSPDYNPDMTNYQVDHILTNEYTTPSCATMLTHGVCVNKDRLCESVSHPLNYYRS
KKKMLEKMRIKDEALAKKAETSAEENPDAGSPGP

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG2219

COG function: function code L; Eukaryotic-type DNA primase, large subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic-type primase large subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007238 [H]

Pfam domain/function: PF04104 DNA_primase_lrg [H]

EC number: NA

Molecular weight: Translated: 39141; Mature: 39141

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSVEKRDLAHYPFLPEAQGLLASRGIKVAGLSGSKIGCSYLDRAAERVITALDGKTVY
CCCCCCHHHCCCCCCCCCHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEC
PESEDDVVSDIITYVLARVLASASKDKRTMDRLCRMEASRVYAYFHAEENELLKKRVCEE
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHH
LGLELGDGALPVIRYVELASRVRDAKWRLINRDVENGFVSVSSDEMDVLIAERIRAILSS
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHC
GLPLDIPESIAKEYLPWSDKILSAVQERTLVEFGAIDESAYPPCIQALISAAAAGVNLTH
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEC
SGRFSMTAFLHNVGMNAEQVAGIFARSPDYNPDMTNYQVDHILTNEYTTPSCATMLTHGV
CCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCC
CVNKDRLCESVSHPLNYYRSKKKMLEKMRIKDEALAKKAETSAEENPDAGSPGP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MRTSVEKRDLAHYPFLPEAQGLLASRGIKVAGLSGSKIGCSYLDRAAERVITALDGKTVY
CCCCCCHHHCCCCCCCCCHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEC
PESEDDVVSDIITYVLARVLASASKDKRTMDRLCRMEASRVYAYFHAEENELLKKRVCEE
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHH
LGLELGDGALPVIRYVELASRVRDAKWRLINRDVENGFVSVSSDEMDVLIAERIRAILSS
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHC
GLPLDIPESIAKEYLPWSDKILSAVQERTLVEFGAIDESAYPPCIQALISAAAAGVNLTH
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEC
SGRFSMTAFLHNVGMNAEQVAGIFARSPDYNPDMTNYQVDHILTNEYTTPSCATMLTHGV
CCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCC
CVNKDRLCESVSHPLNYYRSKKKMLEKMRIKDEALAKKAETSAEENPDAGSPGP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA