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The map label for this gene is eIF2B

Identifier: 124486069

GI number: 124486069

Start: 1274424

End: 1275038

Strand: Reverse

Name: eIF2B

Synonym: Mlab_1249

Alternate gene names: 124486069

Gene position: 1275038-1274424 (Counterclockwise)

Preceding gene: 124486071

Following gene: 124486068

Centisome position: 70.64

GC content: 51.87

Gene sequence:

>615_bases
ATGGTGCAGTCATACGAAGATATGTTAAAGGCCGCGTATTCCGGGATAGGGGAACCGACGGAGACCGGCGAACGGTTCGT
CATGCCGAAGGCGAAGATCTATATCGAGGGGAAAACTACGGTTCTCGAGAACTTTTCCGATATCGTAGACAGTCTCAACC
GTGATAAGGACCACTTCATGAAGTTCATTTTGGGCGAACTTGGTACGGCAGGTAAGATCGACGGAAACCGCGCGGTGTTC
AATGGAAAGTTCGAACAGGCACAGTTTGATGCGATTCTTGAGACATATGTCGGTGATTACGTCATCTGTTCCGAGTGCGG
CAGGCCGGACACGAAACTCGTCAAAGACGACCGCGTACTGATGCTTCTTTGCGAGGCATGCGGATCGAAGAGACCCATCC
GTAAACGCAAAGCGAAGACCGAGGTCCAGGGTCCCGCGATCGAGGAAGGAAAGGAGCTCGAGGTCCATATCGAGTCCATC
AGTAAGAAAGGCGACGGCGTTGCAAGGATCGGCAAATATATTCTGTATGTCGCCGGTACCAAGGCCGGTCAGAATGTGAA
AGTGCGGATCACCCGTATCTCCGGTCAGGTTGCCTTCACTCAGAAAATCCTTTAA

Upstream 100 bases:

>100_bases
TGTCTATGGGGTTATCCCGTGGTTATGCCGGATACATTGGGGTGAGATTCCCGTCCGGTGCATCAGACCTATATGTTCTC
ACAACCAACGGGTATGTATT

Downstream 100 bases:

>100_bases
ATCTTTTTTTTTTGTTATTGATCCATCCCACAATTATATAGTATCGTCCGGCGAATGTATAAGGGAATGAAGCTGAAGAT
TATCGAGCTTGAGAAGGATA

Product: translation initiation factor IF-2 subunit beta

Products: NA

Alternate protein names: aIF2-beta; eIF-2-beta

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MVQSYEDMLKAAYSGIGEPTETGERFVMPKAKIYIEGKTTVLENFSDIVDSLNRDKDHFMKFILGELGTAGKIDGNRAVF
NGKFEQAQFDAILETYVGDYVICSECGRPDTKLVKDDRVLMLLCEACGSKRPIRKRKAKTEVQGPAIEEGKELEVHIESI
SKKGDGVARIGKYILYVAGTKAGQNVKVRITRISGQVAFTQKIL

Sequences:

>Translated_204_residues
MVQSYEDMLKAAYSGIGEPTETGERFVMPKAKIYIEGKTTVLENFSDIVDSLNRDKDHFMKFILGELGTAGKIDGNRAVF
NGKFEQAQFDAILETYVGDYVICSECGRPDTKLVKDDRVLMLLCEACGSKRPIRKRKAKTEVQGPAIEEGKELEVHIESI
SKKGDGVARIGKYILYVAGTKAGQNVKVRITRISGQVAFTQKIL
>Mature_204_residues
MVQSYEDMLKAAYSGIGEPTETGERFVMPKAKIYIEGKTTVLENFSDIVDSLNRDKDHFMKFILGELGTAGKIDGNRAVF
NGKFEQAQFDAILETYVGDYVICSECGRPDTKLVKDDRVLMLLCEACGSKRPIRKRKAKTEVQGPAIEEGKELEVHIESI
SKKGDGVARIGKYILYVAGTKAGQNVKVRITRISGQVAFTQKIL

Specific function: eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA

COG id: COG1601

COG function: function code J; Translation initiation factor 2, beta subunit (eIF-2beta)/eIF-5 N-terminal domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI29826335, Length=134, Percent_Identity=39.5522388059701, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17508049, Length=133, Percent_Identity=35.3383458646617, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6325018, Length=115, Percent_Identity=33.9130434782609, Blast_Score=90, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6325298, Length=120, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=4e-13,
Organism=Drosophila melanogaster, GI17647377, Length=134, Percent_Identity=37.3134328358209, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): IF2B_METLZ (A2SSW2)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001030685.1
- ProteinModelPortal:   A2SSW2
- STRING:   A2SSW2
- GeneID:   4794955
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_1249
- eggNOG:   arNOG13033
- HOGENOM:   HBG738095
- OMA:   ACGAFRP
- ProtClustDB:   PRK12336
- BioCyc:   MLAB410358:MLAB_1249-MONOMER
- HAMAP:   MF_00232
- InterPro:   IPR016027
- InterPro:   IPR004458
- InterPro:   IPR002792
- InterPro:   IPR002735
- InterPro:   IPR016189
- InterPro:   IPR016190
- SMART:   SM00653
- TIGRFAMs:   TIGR00311

Pfam domain/function: PF01873 eIF-5_eIF-2B; PF01938 TRAM; SSF50249 Nucleic_acid_OB; SSF100966 Transl_init_fac_IF2/IF5_N; SSF75689 Transl_init_fac_IF2/IF5_Zn-bd

EC number: NA

Molecular weight: Translated: 22632; Mature: 22632

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQSYEDMLKAAYSGIGEPTETGERFVMPKAKIYIEGKTTVLENFSDIVDSLNRDKDHFM
CCCCHHHHHHHHHHCCCCCCCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHCCCHHHHH
KFILGELGTAGKIDGNRAVFNGKFEQAQFDAILETYVGDYVICSECGRPDTKLVKDDRVL
HHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCEEEECCCCCCCCHHCCCCHHH
MLLCEACGSKRPIRKRKAKTEVQGPAIEEGKELEVHIESISKKGDGVARIGKYILYVAGT
HHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEHHCCCCCCHHHHHHEEEEEECC
KAGQNVKVRITRISGQVAFTQKIL
CCCCCEEEEEEEECCCEEHHHHCC
>Mature Secondary Structure
MVQSYEDMLKAAYSGIGEPTETGERFVMPKAKIYIEGKTTVLENFSDIVDSLNRDKDHFM
CCCCHHHHHHHHHHCCCCCCCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHCCCHHHHH
KFILGELGTAGKIDGNRAVFNGKFEQAQFDAILETYVGDYVICSECGRPDTKLVKDDRVL
HHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCEEEECCCCCCCCHHCCCCHHH
MLLCEACGSKRPIRKRKAKTEVQGPAIEEGKELEVHIESISKKGDGVARIGKYILYVAGT
HHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEHHCCCCCCHHHHHHEEEEEECC
KAGQNVKVRITRISGQVAFTQKIL
CCCCCEEEEEEEECCCEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA