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The map label for this gene is lcfA [H]

Identifier: 124486050

GI number: 124486050

Start: 1257961

End: 1259676

Strand: Reverse

Name: lcfA [H]

Synonym: Mlab_1230

Alternate gene names: 124486050

Gene position: 1259676-1257961 (Counterclockwise)

Preceding gene: 124486051

Following gene: 124486048

Centisome position: 69.79

GC content: 53.38

Gene sequence:

>1716_bases
ATGCTATCCTTCATATGTATATGGTTCATGAACGCCCGCATATACAGAAATGATTCCATCACCAAACTTGAGTATACAAA
CGAGACAAAAAGGTCTCTTTACACGATGCTCACATACGGTGGAACCCATGCAGGACCCCAAGCAATCGCCATACAGTATT
ATAATAATTACATATCATACCGCAGTCTGATGGAGCAGGTACATGCCTGTGCTGCCGGACTCATGCAGCACGGTGTGAAA
AAAGGCGATTTCGTCACCATTTTTCTGCCGAATATCCCGCAAAGCGTTATCGCCGCCTATGCCGTCAACCGGATCGGCGC
CGTCTGCAATCTCGTTCATCCGCTTTCAACGAAAGATGAACTCCGTTACGCGGTGGAACTCACCGAATCCCGGATCGTTC
TGACGTTTGAGATCAACGAAGAACATTGTTCCGGTCTCGATGTCGAGGTCATCCGCTGTAAAACGCCCACCTACTTCCCC
GCAGGCATCAAAGGTCGGATCATGAAGACGGTCTACAACCGTTCGGTTCGTCACGCCAAACAGACGACCGGAAGATCCTC
TGAATGGGACGCTCTTCTCGAAGAAGGACGCGAATTTTTGAAGACCGCGTCCCTTCCCCCGCACGATGTCAGGGCTGAGG
ATACTGCCGTCATCATGTACACCGGCGGCACGACTGGCCCCTCGAAAGGCGTGATGCTTTCAAACACCTCGGCCAATTAT
CTGGCGACCCAGCTTCTCTATGACTATGTGGACGGCAAACCGCATATCGGAGACGGCTTCCTTGCCGTGCTGCCGATCTT
CCATGCGTTTGGTCTGACCGTCTGCATCCACACGCCGCTCTCGTCAGGGATGCGGGTCGCTCTTTGTCCGCGGTTCGATG
CGAAGGAGTGCGCAAAGCTCATCGTAGAAGAGAAGATGGCGTTTCTCTGCGGCGTTCCTGCGATGTACGAGCGGATGTAT
CCGCATCTCAAAGGAAAGGATCTCTCCTGCGTCAAACATCTGGTATGCGGCGGGGACCGGGTCTCTCCCGAGCTTGCCTA
CCGGTATAACGACATTCTCGGAAAAGAAAAAGGCGGAGCAGAGTTCCGTCCGGGCTACGGTCTGACCGAAGCCGGCGGGG
CCTGTGTTATCACCGGCGTGCATTACTCCACGTTAAAGGAAGGGGGCGTGGGTGTCCCTCTTGCCGGAACGGAGATATGC
GTTGTCGCTCCCGGAACGACTGACGTCCTGCCTAACACCGAGGAAGGAGAACTGTGTATGATCGGTCCGGCAATCATGAC
CGGCTACTACAAAAATCCTGAAGAGACCGCCGCTGTTCTCCGCATGCATGACGACGGCCGCATCTGGCTCCACACCGGGG
ACATCGTCTCGATCGGGGAAGGGAACAACATCAATTTCCGGTGCAGATACAAGCGGCTCGTCAAAGTCAACGGATACAAT
GTCTATCCTCTCATGATCGAGGCCGTCATGGAAAAGTGCCCGATCATCTCCCAGTCCTGTGCGATGGGTATTCCCTGGAA
GACGGATACGCGGATCAAACTCTATGTAACGCTGAAGGAGAAGATGGATCCCGAGGCCGCGATAAAAGACATTATCGCCT
TTGCCAAAGCGAATCTGAATCACTGGAGCGTTCCGGTCTCGATCTCGATTCTGGAAGCGATGCCCCTTACCAAAATGAAT
AAAACGGATTATAAAGCCCTGGAAAAGCAGGAATAG

Upstream 100 bases:

>100_bases
GGGCCAATTTCGGGCTGAAGTATAAATACTCTCTGGTCATTGCCTCTGACATTATTTTTTGCAAAATTTGTTCAAATTTT
GCGTGACCGCCAGCTATAAT

Downstream 100 bases:

>100_bases
CTTTTCAAGTTTTGGTAAAAAAAAATATAAATTTATTTTTGGATCGATTTTTTAATTACTGTCAGTCCTGCAGTTGTGAG
GTATGCCACAAAAATCAGGA

Product: cobalamin (vitamin B12) biosynthesis CbiG protein

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MLSFICIWFMNARIYRNDSITKLEYTNETKRSLYTMLTYGGTHAGPQAIAIQYYNNYISYRSLMEQVHACAAGLMQHGVK
KGDFVTIFLPNIPQSVIAAYAVNRIGAVCNLVHPLSTKDELRYAVELTESRIVLTFEINEEHCSGLDVEVIRCKTPTYFP
AGIKGRIMKTVYNRSVRHAKQTTGRSSEWDALLEEGREFLKTASLPPHDVRAEDTAVIMYTGGTTGPSKGVMLSNTSANY
LATQLLYDYVDGKPHIGDGFLAVLPIFHAFGLTVCIHTPLSSGMRVALCPRFDAKECAKLIVEEKMAFLCGVPAMYERMY
PHLKGKDLSCVKHLVCGGDRVSPELAYRYNDILGKEKGGAEFRPGYGLTEAGGACVITGVHYSTLKEGGVGVPLAGTEIC
VVAPGTTDVLPNTEEGELCMIGPAIMTGYYKNPEETAAVLRMHDDGRIWLHTGDIVSIGEGNNINFRCRYKRLVKVNGYN
VYPLMIEAVMEKCPIISQSCAMGIPWKTDTRIKLYVTLKEKMDPEAAIKDIIAFAKANLNHWSVPVSISILEAMPLTKMN
KTDYKALEKQE

Sequences:

>Translated_571_residues
MLSFICIWFMNARIYRNDSITKLEYTNETKRSLYTMLTYGGTHAGPQAIAIQYYNNYISYRSLMEQVHACAAGLMQHGVK
KGDFVTIFLPNIPQSVIAAYAVNRIGAVCNLVHPLSTKDELRYAVELTESRIVLTFEINEEHCSGLDVEVIRCKTPTYFP
AGIKGRIMKTVYNRSVRHAKQTTGRSSEWDALLEEGREFLKTASLPPHDVRAEDTAVIMYTGGTTGPSKGVMLSNTSANY
LATQLLYDYVDGKPHIGDGFLAVLPIFHAFGLTVCIHTPLSSGMRVALCPRFDAKECAKLIVEEKMAFLCGVPAMYERMY
PHLKGKDLSCVKHLVCGGDRVSPELAYRYNDILGKEKGGAEFRPGYGLTEAGGACVITGVHYSTLKEGGVGVPLAGTEIC
VVAPGTTDVLPNTEEGELCMIGPAIMTGYYKNPEETAAVLRMHDDGRIWLHTGDIVSIGEGNNINFRCRYKRLVKVNGYN
VYPLMIEAVMEKCPIISQSCAMGIPWKTDTRIKLYVTLKEKMDPEAAIKDIIAFAKANLNHWSVPVSISILEAMPLTKMN
KTDYKALEKQE
>Mature_571_residues
MLSFICIWFMNARIYRNDSITKLEYTNETKRSLYTMLTYGGTHAGPQAIAIQYYNNYISYRSLMEQVHACAAGLMQHGVK
KGDFVTIFLPNIPQSVIAAYAVNRIGAVCNLVHPLSTKDELRYAVELTESRIVLTFEINEEHCSGLDVEVIRCKTPTYFP
AGIKGRIMKTVYNRSVRHAKQTTGRSSEWDALLEEGREFLKTASLPPHDVRAEDTAVIMYTGGTTGPSKGVMLSNTSANY
LATQLLYDYVDGKPHIGDGFLAVLPIFHAFGLTVCIHTPLSSGMRVALCPRFDAKECAKLIVEEKMAFLCGVPAMYERMY
PHLKGKDLSCVKHLVCGGDRVSPELAYRYNDILGKEKGGAEFRPGYGLTEAGGACVITGVHYSTLKEGGVGVPLAGTEIC
VVAPGTTDVLPNTEEGELCMIGPAIMTGYYKNPEETAAVLRMHDDGRIWLHTGDIVSIGEGNNINFRCRYKRLVKVNGYN
VYPLMIEAVMEKCPIISQSCAMGIPWKTDTRIKLYVTLKEKMDPEAAIKDIIAFAKANLNHWSVPVSISILEAMPLTKMN
KTDYKALEKQE

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=389, Percent_Identity=26.4781491002571, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI187761343, Length=389, Percent_Identity=26.4781491002571, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI156151445, Length=554, Percent_Identity=23.6462093862816, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI12669909, Length=504, Percent_Identity=24.0079365079365, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI4758332, Length=503, Percent_Identity=24.0556660039761, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI42544132, Length=519, Percent_Identity=21.3872832369942, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI28416953, Length=549, Percent_Identity=21.8579234972678, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI38505220, Length=519, Percent_Identity=21.7726396917148, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI157311624, Length=543, Percent_Identity=23.2044198895028, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI157311622, Length=543, Percent_Identity=23.2044198895028, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI58082049, Length=533, Percent_Identity=22.8893058161351, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI13375727, Length=181, Percent_Identity=31.4917127071823, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI122937307, Length=544, Percent_Identity=20.2205882352941, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1788107, Length=531, Percent_Identity=27.3069679849341, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI1789201, Length=368, Percent_Identity=27.445652173913, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI145693145, Length=562, Percent_Identity=23.3096085409253, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI1790505, Length=545, Percent_Identity=23.1192660550459, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1786810, Length=551, Percent_Identity=22.6860254083485, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17559526, Length=509, Percent_Identity=26.9155206286837, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17558820, Length=517, Percent_Identity=24.1779497098646, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI71994694, Length=541, Percent_Identity=24.7689463955638, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71994703, Length=541, Percent_Identity=24.9537892791128, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71994690, Length=541, Percent_Identity=24.9537892791128, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI32563687, Length=363, Percent_Identity=25.068870523416, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17531443, Length=464, Percent_Identity=24.7844827586207, Blast_Score=104, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17560308, Length=379, Percent_Identity=25.065963060686, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17538037, Length=535, Percent_Identity=23.5514018691589, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71996755, Length=524, Percent_Identity=23.4732824427481, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17560140, Length=455, Percent_Identity=23.2967032967033, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71985884, Length=315, Percent_Identity=25.3968253968254, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17564090, Length=362, Percent_Identity=25.414364640884, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323182, Length=552, Percent_Identity=24.4565217391304, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6319264, Length=544, Percent_Identity=23.7132352941176, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6319699, Length=373, Percent_Identity=22.7882037533512, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21355181, Length=519, Percent_Identity=26.3969171483622, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI18859661, Length=489, Percent_Identity=24.1308793456033, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24581924, Length=560, Percent_Identity=24.6428571428571, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21356441, Length=330, Percent_Identity=25.1515151515152, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24656500, Length=525, Percent_Identity=24.952380952381, Blast_Score=96, Evalue=9e-20,
Organism=Drosophila melanogaster, GI21358303, Length=496, Percent_Identity=23.3870967741935, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648253, Length=331, Percent_Identity=23.8670694864048, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648255, Length=331, Percent_Identity=24.1691842900302, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI281365686, Length=332, Percent_Identity=23.1927710843373, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24653035, Length=305, Percent_Identity=25.2459016393443, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24648260, Length=295, Percent_Identity=24.406779661017, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI161076582, Length=356, Percent_Identity=23.0337078651685, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI21356947, Length=292, Percent_Identity=23.6301369863014, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 63271; Mature: 63271

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSFICIWFMNARIYRNDSITKLEYTNETKRSLYTMLTYGGTHAGPQAIAIQYYNNYISY
CCEEEEEEEECCEEEECCCEEEEEECCHHHHHHHHHEEECCCCCCCCEEEEEEHHHHHHH
RSLMEQVHACAAGLMQHGVKKGDFVTIFLPNIPQSVIAAYAVNRIGAVCNLVHPLSTKDE
HHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHH
LRYAVELTESRIVLTFEINEEHCSGLDVEVIRCKTPTYFPAGIKGRIMKTVYNRSVRHAK
HEEEEEECCCEEEEEEEECHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
QTTGRSSEWDALLEEGREFLKTASLPPHDVRAEDTAVIMYTGGTTGPSKGVMLSNTSANY
HHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEECCCHHH
LATQLLYDYVDGKPHIGDGFLAVLPIFHAFGLTVCIHTPLSSGMRVALCPRFDAKECAKL
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCEEEEEECCCCCCCEEEEECCCCHHHHHHH
IVEEKMAFLCGVPAMYERMYPHLKGKDLSCVKHLVCGGDRVSPELAYRYNDILGKEKGGA
HHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCC
EFRPGYGLTEAGGACVITGVHYSTLKEGGVGVPLAGTEICVVAPGTTDVLPNTEEGELCM
CCCCCCCCCCCCCEEEEECCCHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEE
IGPAIMTGYYKNPEETAAVLRMHDDGRIWLHTGDIVSIGEGNNINFRCRYKRLVKVNGYN
ECHHHHHCCCCCHHHHEEEEEEECCCEEEEEECCEEEECCCCCEEEEEEEEEEEEECCEE
VYPLMIEAVMEKCPIISQSCAMGIPWKTDTRIKLYVTLKEKMDPEAAIKDIIAFAKANLN
EHHHHHHHHHHHCCCCCHHCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCC
HWSVPVSISILEAMPLTKMNKTDYKALEKQE
CEECEEEEEEECCCCCCCCCCCHHHHHCCCC
>Mature Secondary Structure
MLSFICIWFMNARIYRNDSITKLEYTNETKRSLYTMLTYGGTHAGPQAIAIQYYNNYISY
CCEEEEEEEECCEEEECCCEEEEEECCHHHHHHHHHEEECCCCCCCCEEEEEEHHHHHHH
RSLMEQVHACAAGLMQHGVKKGDFVTIFLPNIPQSVIAAYAVNRIGAVCNLVHPLSTKDE
HHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHH
LRYAVELTESRIVLTFEINEEHCSGLDVEVIRCKTPTYFPAGIKGRIMKTVYNRSVRHAK
HEEEEEECCCEEEEEEEECHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
QTTGRSSEWDALLEEGREFLKTASLPPHDVRAEDTAVIMYTGGTTGPSKGVMLSNTSANY
HHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEECCCHHH
LATQLLYDYVDGKPHIGDGFLAVLPIFHAFGLTVCIHTPLSSGMRVALCPRFDAKECAKL
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCEEEEEECCCCCCCEEEEECCCCHHHHHHH
IVEEKMAFLCGVPAMYERMYPHLKGKDLSCVKHLVCGGDRVSPELAYRYNDILGKEKGGA
HHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCC
EFRPGYGLTEAGGACVITGVHYSTLKEGGVGVPLAGTEICVVAPGTTDVLPNTEEGELCM
CCCCCCCCCCCCCEEEEECCCHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEE
IGPAIMTGYYKNPEETAAVLRMHDDGRIWLHTGDIVSIGEGNNINFRCRYKRLVKVNGYN
ECHHHHHCCCCCHHHHEEEEEEECCCEEEEEECCEEEECCCCCEEEEEEEEEEEEECCEE
VYPLMIEAVMEKCPIISQSCAMGIPWKTDTRIKLYVTLKEKMDPEAAIKDIIAFAKANLN
EHHHHHHHHHHHCCCCCHHCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCC
HWSVPVSISILEAMPLTKMNKTDYKALEKQE
CEECEEEEEEECCCCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]