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The map label for this gene is gatA

Identifier: 124485961

GI number: 124485961

Start: 1156406

End: 1157680

Strand: Reverse

Name: gatA

Synonym: Mlab_1141

Alternate gene names: 124485961

Gene position: 1157680-1156406 (Counterclockwise)

Preceding gene: 124485962

Following gene: 124485960

Centisome position: 64.14

GC content: 56.78

Gene sequence:

>1275_bases
ATGACCGATATCTATAATGCATATATCAATACGACCGAAGTGAAAGGAAACGGTTCGGGCATTCTTTCCGGCATCCGCGT
CTCCATAAAAGACAATATCTCTACAAAAGACATCGAGACGACCTGCGCCTCCAAAATACTCAAAGGATACATCCCTCCCT
ATGATGCCCACGTCATCACCCGCCTGAAGGCGCAGGGGGCAGTTATTGCCGGAAAGACCAATATGGATGAGTTTGGTATG
GGATCGACCACGGAAAACAGCGCTTTTGGCGCCGCTTTGAATCCGCATGACGTGACCAGAGTTACCGGCGGAAGTTCCGG
TGGTTCTGCCGCGGCGGTTGCCGGCGGTCTCGTCTCAATGGCTCTTGGAAGCGACACGGGGGGCTCGGTCCGCTGTCCGG
CCGCATGGTGCGGTGTTGTCGGTCTGAAACCTTCGTACGGCAGAGTCAGCAGATTCGGTCTGATCGCCTATGCCAACTCT
TTTGAGCAGATCGGCCCGATGACGACCAATGTCCGCGACGCGGCAAAACTGTTCTCCGTGATCGCGGGTCACGACATAAA
GGATTCCACGTCCGTGAACAAACCCTATGACGGAAACGTCGTTCCCGAGATCAGCGGAAAAACGATCGGCATTCCAAAGG
AGTACTTCGGCGAAGGCGTCGACGCCGGCGTTGCGGCCGAGGTCCGTAAAGCGATCGGCAAGCTCGAGGAGCTTGGTGCG
AATATCGTGGATGTATCGCTTCCCTCGATGAAGTATGCACTTCCCGCCTACTATGTGACCTGCACCTGCGAAGCGAGTTC
GAATCTCGGCAGGTTCGACGGGGTCAGGTTCGGTCCGGAGCCGGAGATGAACCTTCCCTGGCACGATGCCTACACCAAAG
TCCGCGAGGCAGGTTTCGGTGCTGAAGTCCGGCGCCGGATCCTTCTTGGGACCTTCGCTCTTTCCGCCGGATACTACGGA
AAATACTACGTCAAGGCCCAGCACGCAAAGCAGCTCATCGCAAAGGATTTCGCAGCGGCTTTAGAGACCTGCGATATGCT
CGCCGGCCCGACCATGCCCAACATTGCGCCCAAGATCGGCGAACTGACCGCCGATCCTCTGCAGATGTATCAGGCGGATA
TCCTGACAGTCCCCGTAAATATCGCCGGGATCCCTGCGATCTCGGTCCCCTGCGGCATCGCCCACAAAATGCCGGTCGGT
CTCCAGCTGATGGGAAGAATGTTCGGCGAGGAGACGATCCTGAATGCCGCTCTTGCCTATGAGGAGGCGGTTTAA

Upstream 100 bases:

>100_bases
CGTGAAGATGAGGTAAAGCCCTCCATTTCCCAAGAGGATGCACTTCGAAACTCTCACAACCCCGAAGACGGATATGTACG
TGCCCCCAAGGTGATCTGAG

Downstream 100 bases:

>100_bases
ATGGCGGATGAGGATTTCACCGTCATCATCGGTCTGGAAGTTCACTGTCAGCTGGATACCGCGTCCAAGCTTTTCTGCGG
ATGTTCGGCCGATTTCCGCG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTDIYNAYINTTEVKGNGSGILSGIRVSIKDNISTKDIETTCASKILKGYIPPYDAHVITRLKAQGAVIAGKTNMDEFGM
GSTTENSAFGAALNPHDVTRVTGGSSGGSAAAVAGGLVSMALGSDTGGSVRCPAAWCGVVGLKPSYGRVSRFGLIAYANS
FEQIGPMTTNVRDAAKLFSVIAGHDIKDSTSVNKPYDGNVVPEISGKTIGIPKEYFGEGVDAGVAAEVRKAIGKLEELGA
NIVDVSLPSMKYALPAYYVTCTCEASSNLGRFDGVRFGPEPEMNLPWHDAYTKVREAGFGAEVRRRILLGTFALSAGYYG
KYYVKAQHAKQLIAKDFAAALETCDMLAGPTMPNIAPKIGELTADPLQMYQADILTVPVNIAGIPAISVPCGIAHKMPVG
LQLMGRMFGEETILNAALAYEEAV

Sequences:

>Translated_424_residues
MTDIYNAYINTTEVKGNGSGILSGIRVSIKDNISTKDIETTCASKILKGYIPPYDAHVITRLKAQGAVIAGKTNMDEFGM
GSTTENSAFGAALNPHDVTRVTGGSSGGSAAAVAGGLVSMALGSDTGGSVRCPAAWCGVVGLKPSYGRVSRFGLIAYANS
FEQIGPMTTNVRDAAKLFSVIAGHDIKDSTSVNKPYDGNVVPEISGKTIGIPKEYFGEGVDAGVAAEVRKAIGKLEELGA
NIVDVSLPSMKYALPAYYVTCTCEASSNLGRFDGVRFGPEPEMNLPWHDAYTKVREAGFGAEVRRRILLGTFALSAGYYG
KYYVKAQHAKQLIAKDFAAALETCDMLAGPTMPNIAPKIGELTADPLQMYQADILTVPVNIAGIPAISVPCGIAHKMPVG
LQLMGRMFGEETILNAALAYEEAV
>Mature_423_residues
TDIYNAYINTTEVKGNGSGILSGIRVSIKDNISTKDIETTCASKILKGYIPPYDAHVITRLKAQGAVIAGKTNMDEFGMG
STTENSAFGAALNPHDVTRVTGGSSGGSAAAVAGGLVSMALGSDTGGSVRCPAAWCGVVGLKPSYGRVSRFGLIAYANSF
EQIGPMTTNVRDAAKLFSVIAGHDIKDSTSVNKPYDGNVVPEISGKTIGIPKEYFGEGVDAGVAAEVRKAIGKLEELGAN
IVDVSLPSMKYALPAYYVTCTCEASSNLGRFDGVRFGPEPEMNLPWHDAYTKVREAGFGAEVRRRILLGTFALSAGYYGK
YYVKAQHAKQLIAKDFAAALETCDMLAGPTMPNIAPKIGELTADPLQMYQADILTVPVNIAGIPAISVPCGIAHKMPVGL
QLMGRMFGEETILNAALAYEEAV

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=465, Percent_Identity=41.505376344086, Blast_Score=307, Evalue=1e-83,
Organism=Homo sapiens, GI195972892, Length=416, Percent_Identity=25, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI166795287, Length=171, Percent_Identity=33.9181286549708, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=403, Percent_Identity=35.7320099255583, Blast_Score=240, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI17537465, Length=422, Percent_Identity=25.3554502369668, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17556264, Length=242, Percent_Identity=30.5785123966942, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71990152, Length=242, Percent_Identity=30.5785123966942, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=180, Percent_Identity=32.2222222222222, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=155, Percent_Identity=32.9032258064516, Blast_Score=65, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17556278, Length=155, Percent_Identity=32.9032258064516, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=419, Percent_Identity=38.1861575178998, Blast_Score=253, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6319685, Length=409, Percent_Identity=28.6063569682152, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6320448, Length=229, Percent_Identity=27.0742358078603, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24648113, Length=423, Percent_Identity=41.8439716312057, Blast_Score=281, Evalue=4e-76,
Organism=Drosophila melanogaster, GI24644968, Length=419, Percent_Identity=26.7303102625298, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24652985, Length=424, Percent_Identity=25, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922090, Length=424, Percent_Identity=25, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24652981, Length=424, Percent_Identity=25, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24652983, Length=424, Percent_Identity=25, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI161078093, Length=420, Percent_Identity=25.952380952381, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21356731, Length=361, Percent_Identity=24.3767313019391, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI45550774, Length=180, Percent_Identity=34.4444444444444, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648435, Length=180, Percent_Identity=34.4444444444444, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648437, Length=180, Percent_Identity=34.4444444444444, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648441, Length=167, Percent_Identity=35.3293413173653, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648439, Length=167, Percent_Identity=35.3293413173653, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 44628; Mature: 44496

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIYNAYINTTEVKGNGSGILSGIRVSIKDNISTKDIETTCASKILKGYIPPYDAHVIT
CCCCEEEEEEEEEECCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHH
RLKAQGAVIAGKTNMDEFGMGSTTENSAFGAALNPHDVTRVTGGSSGGSAAAVAGGLVSM
HHHHCCEEEECCCCCHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHH
ALGSDTGGSVRCPAAWCGVVGLKPSYGRVSRFGLIAYANSFEQIGPMTTNVRDAAKLFSV
HCCCCCCCCEECCHHHHCCCCCCCCCCCHHHHEEEEECCCHHHHCCCCCCHHHHHHHHHH
IAGHDIKDSTSVNKPYDGNVVPEISGKTIGIPKEYFGEGVDAGVAAEVRKAIGKLEELGA
HHCCCCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHCC
NIVDVSLPSMKYALPAYYVTCTCEASSNLGRFDGVRFGPEPEMNLPWHDAYTKVREAGFG
CEEEEECCCHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
AEVRRRILLGTFALSAGYYGKYYVKAQHAKQLIAKDFAAALETCDMLAGPTMPNIAPKIG
HHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
ELTADPLQMYQADILTVPVNIAGIPAISVPCGIAHKMPVGLQLMGRMFGEETILNAALAY
CCCCCHHHHHHCCEEEEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHH
EEAV
HHCC
>Mature Secondary Structure 
TDIYNAYINTTEVKGNGSGILSGIRVSIKDNISTKDIETTCASKILKGYIPPYDAHVIT
CCCEEEEEEEEEECCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHH
RLKAQGAVIAGKTNMDEFGMGSTTENSAFGAALNPHDVTRVTGGSSGGSAAAVAGGLVSM
HHHHCCEEEECCCCCHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHH
ALGSDTGGSVRCPAAWCGVVGLKPSYGRVSRFGLIAYANSFEQIGPMTTNVRDAAKLFSV
HCCCCCCCCEECCHHHHCCCCCCCCCCCHHHHEEEEECCCHHHHCCCCCCHHHHHHHHHH
IAGHDIKDSTSVNKPYDGNVVPEISGKTIGIPKEYFGEGVDAGVAAEVRKAIGKLEELGA
HHCCCCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHCC
NIVDVSLPSMKYALPAYYVTCTCEASSNLGRFDGVRFGPEPEMNLPWHDAYTKVREAGFG
CEEEEECCCHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
AEVRRRILLGTFALSAGYYGKYYVKAQHAKQLIAKDFAAALETCDMLAGPTMPNIAPKIG
HHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
ELTADPLQMYQADILTVPVNIAGIPAISVPCGIAHKMPVGLQLMGRMFGEETILNAALAY
CCCCCHHHHHHCCEEEEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHH
EEAV
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA