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The map label for this gene is gatB

Identifier: 124485960

GI number: 124485960

Start: 1154972

End: 1156405

Strand: Reverse

Name: gatB

Synonym: Mlab_1140

Alternate gene names: 124485960

Gene position: 1156405-1154972 (Counterclockwise)

Preceding gene: 124485961

Following gene: 124485959

Centisome position: 64.07

GC content: 56.35

Gene sequence:

>1434_bases
ATGGCGGATGAGGATTTCACCGTCATCATCGGTCTGGAAGTTCACTGTCAGCTGGATACCGCGTCCAAGCTTTTCTGCGG
ATGTTCGGCCGATTTCCGCGCCGATGCCCCGAACACGCATGTCTGTCCGGTCTGTCTCGGCCTCCCGGGCGCGATGCCCG
TCCTGAACAAAAAGGCGATCGAGTATGCGCTGAAGGTTGGAAAGGCGCTTAACTGCCGGATTCCGGAAGAAGGCGAGTTC
TGCAGAAAGAACTACTTCTACCCCGATCTGGTCAAGGCATATCAGATCACGATGGGCGACAACCCGCTGGCTCTCGGCGG
CTACATCGAGATCGAGGACGACGCAGGCAACCCCAAAGTCGTCAACTTAACGCGGATCCACGTCGAAGAAGATCCAGGTC
GTCTTGTTCACATGGGGAACAAGGAACGCGGGCACTACACCCTCGTTGACTATAACAGATCAGGTATCCCCTTAATCGAA
ATGGTCACCGAGCCGGAACTCTCCTCTCCCAAAGAGGCACGCCGTCTCTTAAACAAACTGCGGGCGACGCTCGAGTATCT
CGACGTCTTCGATCCGGAAAAGGAAGGAAGCATCCGTGTCGATGCGAACATCTCGATCAAAGGACACGAGCGTGTCGAGA
TCAAGAACATCTCCTCCTACAAAGGCGTGGAGAAAGCGCTGACCTTCGAGATCACGAGACAGAAAAATCTGATCAGAAGA
GGCGGCACGATCTCCCGCGAGACCCGTCACTTCCAGGAAGGAAAAGGCACGACCACCTCTGCACGTTCGAAAGAGGTCGC
GAATGATTACCGGTACTTCCCCGAACCGGATCTGCCTGTGATCAGGGTTTCCGACTGGGTGGAAAAGATCGCGCTCCCGG
AGCTGCCGGATGCACGCCGCGACCGGTTCATGTCACAGTACGGCATCGCCGTAAACCATGCGAGAACCCTTACGGGTGAC
CTGCATCTTGCGGAGTTCTACGAACTCGTTGCACCGGTCGGAGCCCAGATCACGGCATCCTGGGTCGCCGACATTCTGAT
CGGCGAACTCAACTACCGCGACATGCCGCTGTCGAAGGTAACCTCCCATGCGGATCAGTTCAAAGAGCTCGTCATCCTTG
TCCGGGACAAGACGATCACCGACAAAGCCGGCGTCGATGTCCTGCGTGTCTGGCTCGATTCCCTGAATGCGGACGGTTCG
GCCGAATCCCCGGCTGGAATCGTTGCACGCCTAGGGCTCATCCGCGAAGCGGGAGAAAGCTTCCGCGGCATCGTTGAAAA
GATCCTCGCCGCAAACCCGCAGGCGATAGCCGATCATAAAGCAGGTAAGAAAGGCGCGTTGAACTTTATCGTCGGTCAGG
TTATGAAGGAAACCAAAGGGAAGTCCGATCCCCGTGAAATCCACGCGATGATTGCTGAAATCCTCGGAGAGTAA

Upstream 100 bases:

>100_bases
CATCGCCCACAAAATGCCGGTCGGTCTCCAGCTGATGGGAAGAATGTTCGGCGAGGAGACGATCCTGAATGCCGCTCTTG
CCTATGAGGAGGCGGTTTAA

Downstream 100 bases:

>100_bases
GTATGCATCTCATCATCGCCGAAAAGAATATTGTTGCCGAGCGAATCGCAGCATTTCTTGCAGGCAAAGCGAAGGTCCAG
GTCACACGGGACGGTGCCGC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MADEDFTVIIGLEVHCQLDTASKLFCGCSADFRADAPNTHVCPVCLGLPGAMPVLNKKAIEYALKVGKALNCRIPEEGEF
CRKNYFYPDLVKAYQITMGDNPLALGGYIEIEDDAGNPKVVNLTRIHVEEDPGRLVHMGNKERGHYTLVDYNRSGIPLIE
MVTEPELSSPKEARRLLNKLRATLEYLDVFDPEKEGSIRVDANISIKGHERVEIKNISSYKGVEKALTFEITRQKNLIRR
GGTISRETRHFQEGKGTTTSARSKEVANDYRYFPEPDLPVIRVSDWVEKIALPELPDARRDRFMSQYGIAVNHARTLTGD
LHLAEFYELVAPVGAQITASWVADILIGELNYRDMPLSKVTSHADQFKELVILVRDKTITDKAGVDVLRVWLDSLNADGS
AESPAGIVARLGLIREAGESFRGIVEKILAANPQAIADHKAGKKGALNFIVGQVMKETKGKSDPREIHAMIAEILGE

Sequences:

>Translated_477_residues
MADEDFTVIIGLEVHCQLDTASKLFCGCSADFRADAPNTHVCPVCLGLPGAMPVLNKKAIEYALKVGKALNCRIPEEGEF
CRKNYFYPDLVKAYQITMGDNPLALGGYIEIEDDAGNPKVVNLTRIHVEEDPGRLVHMGNKERGHYTLVDYNRSGIPLIE
MVTEPELSSPKEARRLLNKLRATLEYLDVFDPEKEGSIRVDANISIKGHERVEIKNISSYKGVEKALTFEITRQKNLIRR
GGTISRETRHFQEGKGTTTSARSKEVANDYRYFPEPDLPVIRVSDWVEKIALPELPDARRDRFMSQYGIAVNHARTLTGD
LHLAEFYELVAPVGAQITASWVADILIGELNYRDMPLSKVTSHADQFKELVILVRDKTITDKAGVDVLRVWLDSLNADGS
AESPAGIVARLGLIREAGESFRGIVEKILAANPQAIADHKAGKKGALNFIVGQVMKETKGKSDPREIHAMIAEILGE
>Mature_476_residues
ADEDFTVIIGLEVHCQLDTASKLFCGCSADFRADAPNTHVCPVCLGLPGAMPVLNKKAIEYALKVGKALNCRIPEEGEFC
RKNYFYPDLVKAYQITMGDNPLALGGYIEIEDDAGNPKVVNLTRIHVEEDPGRLVHMGNKERGHYTLVDYNRSGIPLIEM
VTEPELSSPKEARRLLNKLRATLEYLDVFDPEKEGSIRVDANISIKGHERVEIKNISSYKGVEKALTFEITRQKNLIRRG
GTISRETRHFQEGKGTTTSARSKEVANDYRYFPEPDLPVIRVSDWVEKIALPELPDARRDRFMSQYGIAVNHARTLTGDL
HLAEFYELVAPVGAQITASWVADILIGELNYRDMPLSKVTSHADQFKELVILVRDKTITDKAGVDVLRVWLDSLNADGSA
ESPAGIVARLGLIREAGESFRGIVEKILAANPQAIADHKAGKKGALNFIVGQVMKETKGKSDPREIHAMIAEILGE

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=510, Percent_Identity=33.5294117647059, Blast_Score=256, Evalue=4e-68,
Organism=Caenorhabditis elegans, GI115534094, Length=299, Percent_Identity=35.4515050167224, Blast_Score=192, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6319391, Length=524, Percent_Identity=30.3435114503817, Blast_Score=214, Evalue=3e-56,
Organism=Drosophila melanogaster, GI45550808, Length=497, Percent_Identity=31.3883299798793, Blast_Score=214, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52933; Mature: 52802

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADEDFTVIIGLEVHCQLDTASKLFCGCSADFRADAPNTHVCPVCLGLPGAMPVLNKKAI
CCCCCEEEEEEEEEEEEECCCCCEEECCCCCCCCCCCCCCCCHHEECCCCCCCHHHHHHH
EYALKVGKALNCRIPEEGEFCRKNYFYPDLVKAYQITMGDNPLALGGYIEIEDDAGNPKV
HHHHHHCCEEECCCCCCCCHHHHCCCCHHHHHHEEEEECCCCEEECCEEEEECCCCCCEE
VNLTRIHVEEDPGRLVHMGNKERGHYTLVDYNRSGIPLIEMVTEPELSSPKEARRLLNKL
EEEEEEEEECCCCCEEECCCCCCCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHH
RATLEYLDVFDPEKEGSIRVDANISIKGHERVEIKNISSYKGVEKALTFEITRQKNLIRR
HHHHHHHHCCCCCCCCCEEEEEEEEECCCCEEEEECCCHHCCHHHHHEEEEHHHHHHHHC
GGTISRETRHFQEGKGTTTSARSKEVANDYRYFPEPDLPVIRVSDWVEKIALPELPDARR
CCCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHCCCCCCCHHH
DRFMSQYGIAVNHARTLTGDLHLAEFYELVAPVGAQITASWVADILIGELNYRDMPLSKV
HHHHHHHCEEEECCEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHH
TSHADQFKELVILVRDKTITDKAGVDVLRVWLDSLNADGSAESPAGIVARLGLIREAGES
HHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
FRGIVEKILAANPQAIADHKAGKKGALNFIVGQVMKETKGKSDPREIHAMIAEILGE
HHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADEDFTVIIGLEVHCQLDTASKLFCGCSADFRADAPNTHVCPVCLGLPGAMPVLNKKAI
CCCCEEEEEEEEEEEEECCCCCEEECCCCCCCCCCCCCCCCHHEECCCCCCCHHHHHHH
EYALKVGKALNCRIPEEGEFCRKNYFYPDLVKAYQITMGDNPLALGGYIEIEDDAGNPKV
HHHHHHCCEEECCCCCCCCHHHHCCCCHHHHHHEEEEECCCCEEECCEEEEECCCCCCEE
VNLTRIHVEEDPGRLVHMGNKERGHYTLVDYNRSGIPLIEMVTEPELSSPKEARRLLNKL
EEEEEEEEECCCCCEEECCCCCCCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHH
RATLEYLDVFDPEKEGSIRVDANISIKGHERVEIKNISSYKGVEKALTFEITRQKNLIRR
HHHHHHHHCCCCCCCCCEEEEEEEEECCCCEEEEECCCHHCCHHHHHEEEEHHHHHHHHC
GGTISRETRHFQEGKGTTTSARSKEVANDYRYFPEPDLPVIRVSDWVEKIALPELPDARR
CCCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHCCCCCCCHHH
DRFMSQYGIAVNHARTLTGDLHLAEFYELVAPVGAQITASWVADILIGELNYRDMPLSKV
HHHHHHHCEEEECCEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHH
TSHADQFKELVILVRDKTITDKAGVDVLRVWLDSLNADGSAESPAGIVARLGLIREAGES
HHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
FRGIVEKILAANPQAIADHKAGKKGALNFIVGQVMKETKGKSDPREIHAMIAEILGE
HHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA