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The map label for this gene is pyrG

Identifier: 124485954

GI number: 124485954

Start: 1147440

End: 1149023

Strand: Reverse

Name: pyrG

Synonym: Mlab_1134

Alternate gene names: 124485954

Gene position: 1149023-1147440 (Counterclockwise)

Preceding gene: 124485955

Following gene: 124485953

Centisome position: 63.66

GC content: 54.29

Gene sequence:

>1584_bases
ATGAAATATGTAGTAGTAACCGGAGGAGTAATGAGCGGACTAGGAAAAGGCATCACTGCCGCTTCCATAGGCCGCATTCT
CATCAACAGAGGATATCATGTCACGAGCGTAAAGATCGATCCGTATCTGAATATCGATGCGGGGCTGATGAACCCGGCCC
AGCACGGTGAGGTCTTTGTGTTGAAAGACGGCGGTGAAGCCGATCTTGACCTTGGAAACTATGAGCGGTTTCTTGACATC
GAACTCACCAGCGATCACAATCTGACCACGGGAAAGATCTACTCATCGGTCATCTCGAAGGAGCGCCACGGCGATTATCT
GGGTGCCACCGTTCAGATCATCCCCCATATCACCGACGAAATCAAAGACCGGATCAAACACGCCGCCGAGTCATGGGTCG
ACAAAGACGGCAATCATGCCGAAATATGTATCGTCGAGGTCGGCGGGACCGTGGGCGATATCGAAAGTATGCCGTTTTTG
GAAGCTGTCCGTCAGATGCACTGGGAGTATGGAAACGGAGACTTTGCCCTCGTCCACGTCACCCTTCTGCCTGCCGATAC
GATGGGCGATCTGAAAACGAAACCGACCCAGCACTCAATAAAAGCCCTTCGCGAACTCGGTCTCGAAGCCGACATCATTG
TCGGGAGAAGCAATTTACCGGTCTCCCTTTCCACCAAACGAAAGATCTCCTCCTTCTGCGATGTTGACGTCCACGCGGTC
ATCAGCGCTCAGACGGCACCGGATACCTATCTCGTTCCAATGGAACTCGAGAAAGAGGGAATGGCCGATGTCCTGTTAAA
GCAGCTCCATCTCGAGTCCGGAAAAACGGACAACGGCTGGTATTCTCTGGTTGCACGCGATTACACGAGCCGCATCACTG
TTGGAATCATCACCAAATACGGTGTTGAGGATGTGTATATTTCCATAAAAGAGGCCCTCAAGCACGCAGGCCGCCACCTC
TCCACCGAAGTCGTCATCCACTGGCTGGATGCTGAACTCTACACTCCTGAAGACCTCGCCGATCTTGACGGGATCCTGAT
CCCGGGCGGATTCGGCAACCGCGGTATCGAAGGAATGATCTCGGCGATCCAGTATGCCCGCGAACACAAAAAACCTCTTC
TCGGTCTTTGTCTTGGATTCCAGCTCTGCGTTATCGAGTTCATGAGAAATGTCGTCGGCTACTGGGATGCGACGAGCGAA
GAGATGGGGGCGGGAACCCACGCGATCGCGCTTCTTCCCGAACAGGAAGGCGTTGAAGATCTTGGCGGGACGATGCGTCT
TGGCGACTACCCGGTCACTCTCGATCCGGCTTCCCGCCTCGCAGAGCTTTACGGTGCGACAGAGATCGTGGAACGCCACC
GGCACAGATACGAGGTCAACCCAACCTACATCGACGAGATCGAAGCAAAGGGCATGAAGTTCGTCGGCAAAAACGGCAGA
CGTATGGAGGCGCTCGAACTGGCCGACCACCCATATTTTGTTGCGACCCAGTTCCACCCGGAGTTCAGATCACGTCCGGC
CCGTCCGTCGGCCCCGTTCATCGGTTTTGTAAAAGCCTGCCGTGAGATGAAGAGAAAGGAGTAA

Upstream 100 bases:

>100_bases
TCTCCTGCACCGTCTCTTTCTCCTCTCGATTCCGCATTCTTAATAGCCTTTAAAGTCAATAGATAGACATTATTTGATTT
CGGAAAAGGAAGGGCAAAAA

Downstream 100 bases:

>100_bases
CATGGAATCGATATTAGTCCTTGATTTCGGCGGCCAGTACAACCAGCTGATCTCCCGCCGCGTCAGAGAAGCACATGTTT
TCTGCGAAGTAAAGCCCTGC

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MKYVVVTGGVMSGLGKGITAASIGRILINRGYHVTSVKIDPYLNIDAGLMNPAQHGEVFVLKDGGEADLDLGNYERFLDI
ELTSDHNLTTGKIYSSVISKERHGDYLGATVQIIPHITDEIKDRIKHAAESWVDKDGNHAEICIVEVGGTVGDIESMPFL
EAVRQMHWEYGNGDFALVHVTLLPADTMGDLKTKPTQHSIKALRELGLEADIIVGRSNLPVSLSTKRKISSFCDVDVHAV
ISAQTAPDTYLVPMELEKEGMADVLLKQLHLESGKTDNGWYSLVARDYTSRITVGIITKYGVEDVYISIKEALKHAGRHL
STEVVIHWLDAELYTPEDLADLDGILIPGGFGNRGIEGMISAIQYAREHKKPLLGLCLGFQLCVIEFMRNVVGYWDATSE
EMGAGTHAIALLPEQEGVEDLGGTMRLGDYPVTLDPASRLAELYGATEIVERHRHRYEVNPTYIDEIEAKGMKFVGKNGR
RMEALELADHPYFVATQFHPEFRSRPARPSAPFIGFVKACREMKRKE

Sequences:

>Translated_527_residues
MKYVVVTGGVMSGLGKGITAASIGRILINRGYHVTSVKIDPYLNIDAGLMNPAQHGEVFVLKDGGEADLDLGNYERFLDI
ELTSDHNLTTGKIYSSVISKERHGDYLGATVQIIPHITDEIKDRIKHAAESWVDKDGNHAEICIVEVGGTVGDIESMPFL
EAVRQMHWEYGNGDFALVHVTLLPADTMGDLKTKPTQHSIKALRELGLEADIIVGRSNLPVSLSTKRKISSFCDVDVHAV
ISAQTAPDTYLVPMELEKEGMADVLLKQLHLESGKTDNGWYSLVARDYTSRITVGIITKYGVEDVYISIKEALKHAGRHL
STEVVIHWLDAELYTPEDLADLDGILIPGGFGNRGIEGMISAIQYAREHKKPLLGLCLGFQLCVIEFMRNVVGYWDATSE
EMGAGTHAIALLPEQEGVEDLGGTMRLGDYPVTLDPASRLAELYGATEIVERHRHRYEVNPTYIDEIEAKGMKFVGKNGR
RMEALELADHPYFVATQFHPEFRSRPARPSAPFIGFVKACREMKRKE
>Mature_527_residues
MKYVVVTGGVMSGLGKGITAASIGRILINRGYHVTSVKIDPYLNIDAGLMNPAQHGEVFVLKDGGEADLDLGNYERFLDI
ELTSDHNLTTGKIYSSVISKERHGDYLGATVQIIPHITDEIKDRIKHAAESWVDKDGNHAEICIVEVGGTVGDIESMPFL
EAVRQMHWEYGNGDFALVHVTLLPADTMGDLKTKPTQHSIKALRELGLEADIIVGRSNLPVSLSTKRKISSFCDVDVHAV
ISAQTAPDTYLVPMELEKEGMADVLLKQLHLESGKTDNGWYSLVARDYTSRITVGIITKYGVEDVYISIKEALKHAGRHL
STEVVIHWLDAELYTPEDLADLDGILIPGGFGNRGIEGMISAIQYAREHKKPLLGLCLGFQLCVIEFMRNVVGYWDATSE
EMGAGTHAIALLPEQEGVEDLGGTMRLGDYPVTLDPASRLAELYGATEIVERHRHRYEVNPTYIDEIEAKGMKFVGKNGR
RMEALELADHPYFVATQFHPEFRSRPARPSAPFIGFVKACREMKRKE

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=548, Percent_Identity=47.992700729927, Blast_Score=498, Evalue=1e-141,
Organism=Homo sapiens, GI28559085, Length=547, Percent_Identity=46.9835466179159, Blast_Score=482, Evalue=1e-136,
Organism=Homo sapiens, GI28559083, Length=547, Percent_Identity=46.9835466179159, Blast_Score=482, Evalue=1e-136,
Organism=Homo sapiens, GI221316689, Length=547, Percent_Identity=46.9835466179159, Blast_Score=482, Evalue=1e-136,
Organism=Escherichia coli, GI1789142, Length=548, Percent_Identity=44.5255474452555, Blast_Score=436, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI25148299, Length=601, Percent_Identity=39.2678868552413, Blast_Score=417, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6322563, Length=559, Percent_Identity=46.3327370304114, Blast_Score=481, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6319432, Length=557, Percent_Identity=45.9605026929982, Blast_Score=477, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24664469, Length=547, Percent_Identity=47.1663619744059, Blast_Score=481, Evalue=1e-136,
Organism=Drosophila melanogaster, GI21357815, Length=519, Percent_Identity=43.9306358381503, Blast_Score=409, Evalue=1e-114,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_METLZ (A2SSJ7)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001030570.1
- ProteinModelPortal:   A2SSJ7
- SMR:   A2SSJ7
- STRING:   A2SSJ7
- GeneID:   4795573
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_1134
- eggNOG:   arNOG04675
- HOGENOM:   HBG597806
- OMA:   KIAHFCD
- PhylomeDB:   A2SSJ7
- ProtClustDB:   PRK05380
- BioCyc:   MLAB410358:MLAB_1134-MONOMER
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 58327; Mature: 58327

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS51273 GATASE_TYPE_1

Important sites: ACT_SITE 377-377 ACT_SITE 499-499 ACT_SITE 501-501

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYVVVTGGVMSGLGKGITAASIGRILINRGYHVTSVKIDPYLNIDAGLMNPAQHGEVFV
CEEEEEECHHHHHHCCCCHHHHHHHHHHCCCEEEEEEEECCEEECCCCCCCCCCCCCEEE
LKDGGEADLDLGNYERFLDIELTSDHNLTTGKIYSSVISKERHGDYLGATVQIIPHITDE
EECCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHH
IKDRIKHAAESWVDKDGNHAEICIVEVGGTVGDIESMPFLEAVRQMHWEYGNGDFALVHV
HHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEE
TLLPADTMGDLKTKPTQHSIKALRELGLEADIIVGRSNLPVSLSTKRKISSFCDVDVHAV
EEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEECCHHHHHHHCCCCEEEE
ISAQTAPDTYLVPMELEKEGMADVLLKQLHLESGKTDNGWYSLVARDYTSRITVGIITKY
EEECCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEEHHCCCEEEEEEEEEC
GVEDVYISIKEALKHAGRHLSTEVVIHWLDAELYTPEDLADLDGILIPGGFGNRGIEGMI
CCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHCCCCEEECCCCCCCCHHHHH
SAIQYAREHKKPLLGLCLGFQLCVIEFMRNVVGYWDATSEEMGAGTHAIALLPEQEGVED
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEEECCCCCCHHH
LGGTMRLGDYPVTLDPASRLAELYGATEIVERHRHRYEVNPTYIDEIEAKGMKFVGKNGR
CCCEEEECCEEEEECHHHHHHHHHHHHHHHHHHHHCEECCCCHHHHHHHCCCEEECCCCC
RMEALELADHPYFVATQFHPEFRSRPARPSAPFIGFVKACREMKRKE
EEEEEECCCCCEEEEEEECHHHHCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKYVVVTGGVMSGLGKGITAASIGRILINRGYHVTSVKIDPYLNIDAGLMNPAQHGEVFV
CEEEEEECHHHHHHCCCCHHHHHHHHHHCCCEEEEEEEECCEEECCCCCCCCCCCCCEEE
LKDGGEADLDLGNYERFLDIELTSDHNLTTGKIYSSVISKERHGDYLGATVQIIPHITDE
EECCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHH
IKDRIKHAAESWVDKDGNHAEICIVEVGGTVGDIESMPFLEAVRQMHWEYGNGDFALVHV
HHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEE
TLLPADTMGDLKTKPTQHSIKALRELGLEADIIVGRSNLPVSLSTKRKISSFCDVDVHAV
EEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEECCHHHHHHHCCCCEEEE
ISAQTAPDTYLVPMELEKEGMADVLLKQLHLESGKTDNGWYSLVARDYTSRITVGIITKY
EEECCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEEHHCCCEEEEEEEEEC
GVEDVYISIKEALKHAGRHLSTEVVIHWLDAELYTPEDLADLDGILIPGGFGNRGIEGMI
CCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHCCCCEEECCCCCCCCHHHHH
SAIQYAREHKKPLLGLCLGFQLCVIEFMRNVVGYWDATSEEMGAGTHAIALLPEQEGVED
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEEECCCCCCHHH
LGGTMRLGDYPVTLDPASRLAELYGATEIVERHRHRYEVNPTYIDEIEAKGMKFVGKNGR
CCCEEEECCEEEEECHHHHHHHHHHHHHHHHHHHHCEECCCCHHHHHHHCCCEEECCCCC
RMEALELADHPYFVATQFHPEFRSRPARPSAPFIGFVKACREMKRKE
EEEEEECCCCCEEEEEEECHHHHCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA