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The map label for this gene is lipB [H]

Identifier: 124485836

GI number: 124485836

Start: 1025385

End: 1026104

Strand: Reverse

Name: lipB [H]

Synonym: Mlab_1016

Alternate gene names: 124485836

Gene position: 1026104-1025385 (Counterclockwise)

Preceding gene: 124485837

Following gene: 124485835

Centisome position: 56.85

GC content: 52.78

Gene sequence:

>720_bases
ATGAAGGTGACGGCGCTTGGAACCGGAGACGTTGTCGGCACGCCGAAAATCGGATGTGAGTGTCCAATCTGCCGTGAAGC
AAAGGAAATGGGAAAACAACGGCTTCGAACTTCGCTGTTAGTTGAGCACAAAGGCCGCCATCTCTTAATTGACACAGGAC
CGGATATGCGTGCTCAACTGCTGGCCGCCGACTCCCCGCGAATCGACGCCGTCATCTGGACCCATGGACATTATGATCAT
TTCATGGGGTTCGGCGACTTTTACCGGGTCCAGCGGGAACCGATCTCGGTTTACGGGGCACCCGAGGTGCTGGCGTACTG
CGGGGGGATATTTTGTTTCATCCGTCACGAAGAACACTCGGTCAAACCGTTTGAACCAATCGATATCTGCGGGATGGAAG
TAACACTCTTTCCCGTCGTTCACGACACTCCGACCTACGGTGTTCGGATCACTGCCGACGGCAAAACGTTCGTCTACTCC
TGCGATACACGTGCCGAACTTTCCGAAGCTTCGCTTGAGCAGATGAAAGGCGCTGATCTTTTACTGCTCGACGGCATATT
CCCGCCGGGAGTCAACATCTCCAAACACATGAACATTGAGGATGCCGAAAGACTTGCCGAAAATCTGGCTCCAAAAGAGT
TCTGGTGCGTTCATATGAGTCATAAAATACCCTGGGACTATGAGCATGCCGCTTTTGACATGCAGTCCTGGACTCTGTAA

Upstream 100 bases:

>100_bases
CGGCTCTTCTTTCCGGAAGAAAGGAGATCAACGAAGCCGACGTGAATGCAGGAATGGAACTGGTCAACCGGAGAAATCTG
GTTCGCAGCGGGTGCACCTG

Downstream 100 bases:

>100_bases
TTTTTTAATCTCAGTCGGCAAGTTTATCACATTTAGCGTACACTTAACTATTAGCGCTTTTAGCTGTTTCAATGAAAACA
GTGTTTTATGAATTTGCAGC

Product: hypothetical protein

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKVTALGTGDVVGTPKIGCECPICREAKEMGKQRLRTSLLVEHKGRHLLIDTGPDMRAQLLAADSPRIDAVIWTHGHYDH
FMGFGDFYRVQREPISVYGAPEVLAYCGGIFCFIRHEEHSVKPFEPIDICGMEVTLFPVVHDTPTYGVRITADGKTFVYS
CDTRAELSEASLEQMKGADLLLLDGIFPPGVNISKHMNIEDAERLAENLAPKEFWCVHMSHKIPWDYEHAAFDMQSWTL

Sequences:

>Translated_239_residues
MKVTALGTGDVVGTPKIGCECPICREAKEMGKQRLRTSLLVEHKGRHLLIDTGPDMRAQLLAADSPRIDAVIWTHGHYDH
FMGFGDFYRVQREPISVYGAPEVLAYCGGIFCFIRHEEHSVKPFEPIDICGMEVTLFPVVHDTPTYGVRITADGKTFVYS
CDTRAELSEASLEQMKGADLLLLDGIFPPGVNISKHMNIEDAERLAENLAPKEFWCVHMSHKIPWDYEHAAFDMQSWTL
>Mature_239_residues
MKVTALGTGDVVGTPKIGCECPICREAKEMGKQRLRTSLLVEHKGRHLLIDTGPDMRAQLLAADSPRIDAVIWTHGHYDH
FMGFGDFYRVQREPISVYGAPEVLAYCGGIFCFIRHEEHSVKPFEPIDICGMEVTLFPVVHDTPTYGVRITADGKTFVYS
CDTRAELSEASLEQMKGADLLLLDGIFPPGVNISKHMNIEDAERLAENLAPKEFWCVHMSHKIPWDYEHAAFDMQSWTL

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG1235

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily I

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the lipB family [H]

Homologues:

Organism=Escherichia coli, GI1790530, Length=225, Percent_Identity=26.6666666666667, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF00753 Lactamase_B [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 26879; Mature: 26879

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTALGTGDVVGTPKIGCECPICREAKEMGKQRLRTSLLVEHKGRHLLIDTGPDMRAQL
CEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCEEEE
LAADSPRIDAVIWTHGHYDHFMGFGDFYRVQREPISVYGAPEVLAYCGGIFCFIRHEEHS
EECCCCCEEEEEEECCCHHHHCCCCHHEEEECCCEEEECCHHHHHHHCEEEEEEEECCCC
VKPFEPIDICGMEVTLFPVVHDTPTYGVRITADGKTFVYSCDTRAELSEASLEQMKGADL
CCCCCCCEECCCEEEEEEEEECCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHCCCCE
LLLDGIFPPGVNISKHMNIEDAERLAENLAPKEFWCVHMSHKIPWDYEHAAFDMQSWTL
EEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHEECCHHCCC
>Mature Secondary Structure
MKVTALGTGDVVGTPKIGCECPICREAKEMGKQRLRTSLLVEHKGRHLLIDTGPDMRAQL
CEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCEEEE
LAADSPRIDAVIWTHGHYDHFMGFGDFYRVQREPISVYGAPEVLAYCGGIFCFIRHEEHS
EECCCCCEEEEEEECCCHHHHCCCCHHEEEECCCEEEECCHHHHHHHCEEEEEEEECCCC
VKPFEPIDICGMEVTLFPVVHDTPTYGVRITADGKTFVYSCDTRAELSEASLEQMKGADL
CCCCCCCEECCCEEEEEEEEECCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHCCCCE
LLLDGIFPPGVNISKHMNIEDAERLAENLAPKEFWCVHMSHKIPWDYEHAAFDMQSWTL
EEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12949112 [H]