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The map label for this gene is purH [H]

Identifier: 124485762

GI number: 124485762

Start: 938133

End: 939626

Strand: Reverse

Name: purH [H]

Synonym: Mlab_0941

Alternate gene names: 124485762

Gene position: 939626-938133 (Counterclockwise)

Preceding gene: 124485763

Following gene: 124485761

Centisome position: 52.06

GC content: 54.42

Gene sequence:

>1494_bases
ATGACTCTTGCACTTCTGTCAGTCTGGGACAAGACCGGGATCCTTGATCTCGCCCGTGCCCTGGTTGCAAAAAACATTGG
AATCCTAAGTTCAGGCGGAACGGCAAAGGCTCTGCGTGAAGCGGGCATTCCTGCAAAAGACGTTTCAGAATACACGCAGT
TTCCCGAGATGATGGACGGGCGTGTAAAGACCCTTCACCCGAAGGTCCACGGCGGTCTTCTTGGTCGCAGAGGCATCGAT
GACGATGTTATGAAGGCCCACTTCATTGAACCGATCGACATCCTTTGCGTCAATCTGTATCCGTTCGAGGAGATGTCCAA
GAAAAATCTCCCGTTAGAGGAGCTCATCGAGTTCATCGATATCGGCGGCCCGGCGATGATCCGTGCGGCATCCAAGAACT
ACAAGGATGTTGCTGTTTTGACCGATCCTTCCGATTATCCTATGGCGATCGAAGCGATCAAAACCGGCGGCTTCACTTCA
GAGCAGAAACTTCGTCTTGCGACCAAGGCATTCACTCGAACAGCCGCATACGATGCTGCGATCTCTAATTATCTCAATGG
AATCGACAAGGAGTTCCCGGATGTCTACACCATGCAGTTTGGCAACGGCAGAAAACTCAGATACGGAGAGAACCCTCACC
AGAAAGCAGCCGTCTACGGTACATCCGGCATCGCCGGTCAGGTCGCTCTGCAGGGCAAAGAGATGTCCTACAACAACTAC
CTCGATGTCCACGCCGCGGTCAGTCTCTGCAGAGAACTCCCGGGTTTTGCGACCGTAATCGTAAAACACAACAATCCGTG
CGGGGTTGCTCTTGGGAAAAACCAGCTCGAATCATACATCAAGGCGCGGGATGTCGACCCAGTCTCGGCTTACGGTTCCA
TCGTAGCTATGAGCACGCCTGTCGATACCGATATCGCCAAGGAGATCTGTTCAACCTTCGTCGAGGTCCTGATCGCTCCG
TCGTTCAGCGACGAGGCACGCGAGATGATGAAGAAAAAGGAGAACATGCGTCTCTTAATTCTCCCGCCAGCTGAGCCTGC
CGATGAGATCCGCACGATCGACGGCGGTATTCTCGTCCAGAGAACCCCTGCCTACACCGAGGACTGGAAGGTCGTTTCCA
AGCGTGCTCCAACCCCGGACGAGGTCGAGGCACTCAAACTCGCCTGGAAGGTGGTTGAATACGCAAAGAGTAATGCCATC
ATCTACGCGAACAAAACCGAGACGGTCGGTATCGGTGTCGGTCAGATGAACCGTGTCGATTCAGCAAAACTGGCCATTCA
GAAAGCTGCCGAGTTCGGCAGAACGATGCAGGGAACCGTGATTGCCTCCGATGCCTTCCTGCCGTTTTCAGACACGCTGG
AAGTGGCGGCTGCCGCGGGAGCGACGGCACTCATCCAGCCCGGCGGCTCGATCCGTGATGATGAAGTGCTTGCAAAGGCC
GATGAACTCGGTATCGCGATGGTGTTCACCGGCGTCCGTCACTTCAGACATTAA

Upstream 100 bases:

>100_bases
ATCGACCCCGGTTCTCTCACTGATTTTTTGATAGGTTTATCACACATTCTTAGGGGGAAGTGCTACCCTTAATTAACCCT
CGCGTCCATAGTAATAGATA

Downstream 100 bases:

>100_bases
ATATTGCTTTTTACGGGGATCTTTTCCCCTGCTTTTTTGGTTACTGTAAGTAATTTACTATGGCCGAATCGTGTTGTTTG
TTCCTCAATTATTATGCGAG

Product: IMP cyclohydrolase / phosphoribosylaminoimidazolecarboxamide formyltransferase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MTLALLSVWDKTGILDLARALVAKNIGILSSGGTAKALREAGIPAKDVSEYTQFPEMMDGRVKTLHPKVHGGLLGRRGID
DDVMKAHFIEPIDILCVNLYPFEEMSKKNLPLEELIEFIDIGGPAMIRAASKNYKDVAVLTDPSDYPMAIEAIKTGGFTS
EQKLRLATKAFTRTAAYDAAISNYLNGIDKEFPDVYTMQFGNGRKLRYGENPHQKAAVYGTSGIAGQVALQGKEMSYNNY
LDVHAAVSLCRELPGFATVIVKHNNPCGVALGKNQLESYIKARDVDPVSAYGSIVAMSTPVDTDIAKEICSTFVEVLIAP
SFSDEAREMMKKKENMRLLILPPAEPADEIRTIDGGILVQRTPAYTEDWKVVSKRAPTPDEVEALKLAWKVVEYAKSNAI
IYANKTETVGIGVGQMNRVDSAKLAIQKAAEFGRTMQGTVIASDAFLPFSDTLEVAAAAGATALIQPGGSIRDDEVLAKA
DELGIAMVFTGVRHFRH

Sequences:

>Translated_497_residues
MTLALLSVWDKTGILDLARALVAKNIGILSSGGTAKALREAGIPAKDVSEYTQFPEMMDGRVKTLHPKVHGGLLGRRGID
DDVMKAHFIEPIDILCVNLYPFEEMSKKNLPLEELIEFIDIGGPAMIRAASKNYKDVAVLTDPSDYPMAIEAIKTGGFTS
EQKLRLATKAFTRTAAYDAAISNYLNGIDKEFPDVYTMQFGNGRKLRYGENPHQKAAVYGTSGIAGQVALQGKEMSYNNY
LDVHAAVSLCRELPGFATVIVKHNNPCGVALGKNQLESYIKARDVDPVSAYGSIVAMSTPVDTDIAKEICSTFVEVLIAP
SFSDEAREMMKKKENMRLLILPPAEPADEIRTIDGGILVQRTPAYTEDWKVVSKRAPTPDEVEALKLAWKVVEYAKSNAI
IYANKTETVGIGVGQMNRVDSAKLAIQKAAEFGRTMQGTVIASDAFLPFSDTLEVAAAAGATALIQPGGSIRDDEVLAKA
DELGIAMVFTGVRHFRH
>Mature_496_residues
TLALLSVWDKTGILDLARALVAKNIGILSSGGTAKALREAGIPAKDVSEYTQFPEMMDGRVKTLHPKVHGGLLGRRGIDD
DVMKAHFIEPIDILCVNLYPFEEMSKKNLPLEELIEFIDIGGPAMIRAASKNYKDVAVLTDPSDYPMAIEAIKTGGFTSE
QKLRLATKAFTRTAAYDAAISNYLNGIDKEFPDVYTMQFGNGRKLRYGENPHQKAAVYGTSGIAGQVALQGKEMSYNNYL
DVHAAVSLCRELPGFATVIVKHNNPCGVALGKNQLESYIKARDVDPVSAYGSIVAMSTPVDTDIAKEICSTFVEVLIAPS
FSDEAREMMKKKENMRLLILPPAEPADEIRTIDGGILVQRTPAYTEDWKVVSKRAPTPDEVEALKLAWKVVEYAKSNAII
YANKTETVGIGVGQMNRVDSAKLAIQKAAEFGRTMQGTVIASDAFLPFSDTLEVAAAAGATALIQPGGSIRDDEVLAKAD
ELGIAMVFTGVRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=472, Percent_Identity=35.3813559322034, Blast_Score=235, Evalue=8e-62,
Organism=Escherichia coli, GI1790439, Length=520, Percent_Identity=48.8461538461538, Blast_Score=471, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI71985564, Length=509, Percent_Identity=33.5952848722986, Blast_Score=239, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI71985574, Length=346, Percent_Identity=26.5895953757225, Blast_Score=85, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6323056, Length=465, Percent_Identity=38.2795698924731, Blast_Score=254, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6323768, Length=469, Percent_Identity=38.1663113006397, Blast_Score=249, Evalue=9e-67,
Organism=Drosophila melanogaster, GI24649832, Length=467, Percent_Identity=38.1156316916488, Blast_Score=249, Evalue=3e-66,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 53978; Mature: 53846

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLALLSVWDKTGILDLARALVAKNIGILSSGGTAKALREAGIPAKDVSEYTQFPEMMDG
CEEEEEEHHCCCCHHHHHHHHHHHCCCEEECCCHHHHHHHCCCCHHHHHHHHHCHHHHCC
RVKTLHPKVHGGLLGRRGIDDDVMKAHFIEPIDILCVNLYPFEEMSKKNLPLEELIEFID
CEEEECCHHHCCCCCCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHCCCCCHHHHHHHHH
IGGPAMIRAASKNYKDVAVLTDPSDYPMAIEAIKTGGFTSEQKLRLATKAFTRTAAYDAA
CCCHHEEEECCCCCCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
ISNYLNGIDKEFPDVYTMQFGNGRKLRYGENPHQKAAVYGTSGIAGQVALQGKEMSYNNY
HHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCE
LDVHAAVSLCRELPGFATVIVKHNNPCGVALGKNQLESYIKARDVDPVSAYGSIVAMSTP
EHHHHHHHHHHHCCCEEEEEEEECCCCEEEECHHHHHHHHHHCCCCCHHHCCCEEEECCC
VDTDIAKEICSTFVEVLIAPSFSDEAREMMKKKENMRLLILPPAEPADEIRTIDGGILVQ
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHEECCCCEEEE
RTPAYTEDWKVVSKRAPTPDEVEALKLAWKVVEYAKSNAIIYANKTETVGIGVGQMNRVD
ECCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCCCCH
SAKLAIQKAAEFGRTMQGTVIASDAFLPFSDTLEVAAAAGATALIQPGGSIRDDEVLAKA
HHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHCCCEEEECCCCCCCCHHHHHHH
DELGIAMVFTGVRHFRH
HHCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TLALLSVWDKTGILDLARALVAKNIGILSSGGTAKALREAGIPAKDVSEYTQFPEMMDG
EEEEEEHHCCCCHHHHHHHHHHHCCCEEECCCHHHHHHHCCCCHHHHHHHHHCHHHHCC
RVKTLHPKVHGGLLGRRGIDDDVMKAHFIEPIDILCVNLYPFEEMSKKNLPLEELIEFID
CEEEECCHHHCCCCCCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHCCCCCHHHHHHHHH
IGGPAMIRAASKNYKDVAVLTDPSDYPMAIEAIKTGGFTSEQKLRLATKAFTRTAAYDAA
CCCHHEEEECCCCCCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
ISNYLNGIDKEFPDVYTMQFGNGRKLRYGENPHQKAAVYGTSGIAGQVALQGKEMSYNNY
HHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCE
LDVHAAVSLCRELPGFATVIVKHNNPCGVALGKNQLESYIKARDVDPVSAYGSIVAMSTP
EHHHHHHHHHHHCCCEEEEEEEECCCCEEEECHHHHHHHHHHCCCCCHHHCCCEEEECCC
VDTDIAKEICSTFVEVLIAPSFSDEAREMMKKKENMRLLILPPAEPADEIRTIDGGILVQ
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHEECCCCEEEE
RTPAYTEDWKVVSKRAPTPDEVEALKLAWKVVEYAKSNAIIYANKTETVGIGVGQMNRVD
ECCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCCCCH
SAKLAIQKAAEFGRTMQGTVIASDAFLPFSDTLEVAAAAGATALIQPGGSIRDDEVLAKA
HHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHCCCEEEECCCCCCCCHHHHHHH
DELGIAMVFTGVRHFRH
HHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA