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The map label for this gene is opuE [H]

Identifier: 124485686

GI number: 124485686

Start: 853889

End: 855463

Strand: Reverse

Name: opuE [H]

Synonym: Mlab_0864

Alternate gene names: 124485686

Gene position: 855463-853889 (Counterclockwise)

Preceding gene: 124485687

Following gene: 124485683

Centisome position: 47.4

GC content: 52.57

Gene sequence:

>1575_bases
ATGGCACTGGCTGAAGGAAGCACATTTATTACGATCGCGCTCATCGCTGTTTACTTTGTAGTTTTACTCGGTATCGGCGT
ATGGGCATCGAAGAAGATCAAAAATTCAGAAGACTATATGCTGGCCGGCAGAAGTCTCGGGTTCTGGCTGTTTACCCTGT
TGATCGTCTGTTCGATCTGCAGCGGTATGACGCTTGTTGGAACCAGCGGGTTTGGGTTTGCGTCAGGCTGGCCGGGCATT
TGGGAACAGATTTTCGTTCCATTGTCGGCGGCGTTTTGTATCATCTTCTTTGGTTCAAAACTCAATAAGATCGGCAGAAA
AAGCGGGTGCATGACCATTGTGGATTATTTTGCACTCAGATATGAAAATACGCGGGAGCTTCGCACTCTTTCTGCAATCG
CAAGCATTGCAGTTTCACTGGTATATCTCGTCGGGCAGTACACCGCCATCAGTATCGTTTTGATCTGGGCGTTCGGGCTG
GAACACTGGCAGGCCCTGCTGATCGCAGGGGTCATCATCACCGCATATACCGTTATCGGCGGCCTGTATGCCGTCTCCTG
GACGGGACTCGTTCAGGGTCTGATTCTTATCTTCGGCGTGATTCTCGTAGCACCGTTCATCCTGACCTATGCAGGAGGTC
TCGAGCATATTAACACGGTTCTCGCAACGATCGATCCGAATCTGGTCATGCCCTGGTTCCCGTCGGGAAGCACGGAAGCA
TACGCCTATTGTACACCTGAATATCTGTTCTCGTTTGGCGTGCTGCTGATTGTAGGTCTCGCCTGTGCACCGCACGTTCT
CTCGAACGTGTTTGCTGCGAGAAAAACGTCCTACTTCAAGTGGGCGCCGCTTGTGGCATTCTCGATCTACCTCGTGATCA
TGCTTTTGATCAAGATCTGCGGTATGGCGGTCAGATCCATGCAGGCGGACGGCCTCCTTTCCGGGATTGTGCCGGAAGGC
GGACGGGTCGCGGACTTTGCACTGATGTATGGTATTCAGGCGGCAAGTCCAAGCATCCTGATCACGGGTCTGTTTGCGGT
GATCGTTCTCTCGGCAGTTATGTCGACGACCGACCGGCTTATGCTGACGGTTGGAACGACCTTCTCCTGGGATATCTTCA
AGACGATGCTCAAACCGATGGCAAGCGAACGTCAGGTGGTTCTCGTGAGTCAGATCTGCGTTCTTCTGGCGGCAGTCATC
TCGCTGATTCTGGCAATCAACCCGCCGGAGATGCTCGCCTTCCTGATCTGGCTCGGTATCGGCCTGATGCTTTCAGCATT
CGCCGTGCCAATGATCGCCGGTCTCTACTGGAGACGGGCGACCGCAGCGGGCGCGATTGCGAGTATGCTCGTTGGTCTTA
TCACCTGTCTCGCGTTCGGCGCGTACTACAACTTCATCGACAAGCTGCCGATGCACTTCTCAATCTATTCACTGCTCTGT
TCAATCGCCGCGATGGTTATCGTCAGTCTGCTTACGAAGAAGAATTCCGAGAAGGCACTTGATCTGACCTACACAGGCAT
GTATCTTCATCCAAAGGATGAACCACACTCTTCGACTGAACAGTCAAAAGAGTAA

Upstream 100 bases:

>100_bases
CCGATATCACGGTTTTCACTGTTGGCGGAGCCATCATTTTATGTATCATTCTCCTATTAATTTGGGGCATCACATTCCCT
AAGGAGAATGACGACTAAAG

Downstream 100 bases:

>100_bases
TTTCTTTTTTTTTACGCGAACGTTTGAAGCAGTGTGAAAAGCACGCGAAAGGAACGATTTTTTTGAAGAGAAGTTACGCG
GCGTTATCCTCAATCCAATT

Product: hypothetical protein

Products: Na (I) [Cytoplasm]; pantothenate [Cytoplasm] [C]

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MALAEGSTFITIALIAVYFVVLLGIGVWASKKIKNSEDYMLAGRSLGFWLFTLLIVCSICSGMTLVGTSGFGFASGWPGI
WEQIFVPLSAAFCIIFFGSKLNKIGRKSGCMTIVDYFALRYENTRELRTLSAIASIAVSLVYLVGQYTAISIVLIWAFGL
EHWQALLIAGVIITAYTVIGGLYAVSWTGLVQGLILIFGVILVAPFILTYAGGLEHINTVLATIDPNLVMPWFPSGSTEA
YAYCTPEYLFSFGVLLIVGLACAPHVLSNVFAARKTSYFKWAPLVAFSIYLVIMLLIKICGMAVRSMQADGLLSGIVPEG
GRVADFALMYGIQAASPSILITGLFAVIVLSAVMSTTDRLMLTVGTTFSWDIFKTMLKPMASERQVVLVSQICVLLAAVI
SLILAINPPEMLAFLIWLGIGLMLSAFAVPMIAGLYWRRATAAGAIASMLVGLITCLAFGAYYNFIDKLPMHFSIYSLLC
SIAAMVIVSLLTKKNSEKALDLTYTGMYLHPKDEPHSSTEQSKE

Sequences:

>Translated_524_residues
MALAEGSTFITIALIAVYFVVLLGIGVWASKKIKNSEDYMLAGRSLGFWLFTLLIVCSICSGMTLVGTSGFGFASGWPGI
WEQIFVPLSAAFCIIFFGSKLNKIGRKSGCMTIVDYFALRYENTRELRTLSAIASIAVSLVYLVGQYTAISIVLIWAFGL
EHWQALLIAGVIITAYTVIGGLYAVSWTGLVQGLILIFGVILVAPFILTYAGGLEHINTVLATIDPNLVMPWFPSGSTEA
YAYCTPEYLFSFGVLLIVGLACAPHVLSNVFAARKTSYFKWAPLVAFSIYLVIMLLIKICGMAVRSMQADGLLSGIVPEG
GRVADFALMYGIQAASPSILITGLFAVIVLSAVMSTTDRLMLTVGTTFSWDIFKTMLKPMASERQVVLVSQICVLLAAVI
SLILAINPPEMLAFLIWLGIGLMLSAFAVPMIAGLYWRRATAAGAIASMLVGLITCLAFGAYYNFIDKLPMHFSIYSLLC
SIAAMVIVSLLTKKNSEKALDLTYTGMYLHPKDEPHSSTEQSKE
>Mature_523_residues
ALAEGSTFITIALIAVYFVVLLGIGVWASKKIKNSEDYMLAGRSLGFWLFTLLIVCSICSGMTLVGTSGFGFASGWPGIW
EQIFVPLSAAFCIIFFGSKLNKIGRKSGCMTIVDYFALRYENTRELRTLSAIASIAVSLVYLVGQYTAISIVLIWAFGLE
HWQALLIAGVIITAYTVIGGLYAVSWTGLVQGLILIFGVILVAPFILTYAGGLEHINTVLATIDPNLVMPWFPSGSTEAY
AYCTPEYLFSFGVLLIVGLACAPHVLSNVFAARKTSYFKWAPLVAFSIYLVIMLLIKICGMAVRSMQADGLLSGIVPEGG
RVADFALMYGIQAASPSILITGLFAVIVLSAVMSTTDRLMLTVGTTFSWDIFKTMLKPMASERQVVLVSQICVLLAAVIS
LILAINPPEMLAFLIWLGIGLMLSAFAVPMIAGLYWRRATAAGAIASMLVGLITCLAFGAYYNFIDKLPMHFSIYSLLCS
IAAMVIVSLLTKKNSEKALDLTYTGMYLHPKDEPHSSTEQSKE

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=511, Percent_Identity=25.2446183953033, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI14140236, Length=422, Percent_Identity=24.8815165876777, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI110835708, Length=571, Percent_Identity=23.9929947460595, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI11141885, Length=398, Percent_Identity=24.8743718592965, Blast_Score=78, Evalue=3e-14,
Organism=Homo sapiens, GI109659836, Length=479, Percent_Identity=24.0083507306889, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI4507033, Length=413, Percent_Identity=23.0024213075061, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI87082237, Length=454, Percent_Identity=26.2114537444934, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1790503, Length=456, Percent_Identity=24.780701754386, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1787251, Length=517, Percent_Identity=25.9187620889749, Blast_Score=106, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24648033, Length=409, Percent_Identity=24.2053789731051, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356865, Length=409, Percent_Identity=24.2053789731051, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 56761; Mature: 56630

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS00457 NA_SOLUT_SYMP_2 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAEGSTFITIALIAVYFVVLLGIGVWASKKIKNSEDYMLAGRSLGFWLFTLLIVCSIC
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHHH
SGMTLVGTSGFGFASGWPGIWEQIFVPLSAAFCIIFFGSKLNKIGRKSGCMTIVDYFALR
CCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
YENTRELRTLSAIASIAVSLVYLVGQYTAISIVLIWAFGLEHWQALLIAGVIITAYTVIG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLYAVSWTGLVQGLILIFGVILVAPFILTYAGGLEHINTVLATIDPNLVMPWFPSGSTEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEECCCCCCCCE
YAYCTPEYLFSFGVLLIVGLACAPHVLSNVFAARKTSYFKWAPLVAFSIYLVIMLLIKIC
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
GMAVRSMQADGLLSGIVPEGGRVADFALMYGIQAASPSILITGLFAVIVLSAVMSTTDRL
HHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCE
MLTVGTTFSWDIFKTMLKPMASERQVVLVSQICVLLAAVISLILAINPPEMLAFLIWLGI
EEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GLMLSAFAVPMIAGLYWRRATAAGAIASMLVGLITCLAFGAYYNFIDKLPMHFSIYSLLC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SIAAMVIVSLLTKKNSEKALDLTYTGMYLHPKDEPHSSTEQSKE
HHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
ALAEGSTFITIALIAVYFVVLLGIGVWASKKIKNSEDYMLAGRSLGFWLFTLLIVCSIC
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHHH
SGMTLVGTSGFGFASGWPGIWEQIFVPLSAAFCIIFFGSKLNKIGRKSGCMTIVDYFALR
CCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
YENTRELRTLSAIASIAVSLVYLVGQYTAISIVLIWAFGLEHWQALLIAGVIITAYTVIG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLYAVSWTGLVQGLILIFGVILVAPFILTYAGGLEHINTVLATIDPNLVMPWFPSGSTEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEECCCCCCCCE
YAYCTPEYLFSFGVLLIVGLACAPHVLSNVFAARKTSYFKWAPLVAFSIYLVIMLLIKIC
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
GMAVRSMQADGLLSGIVPEGGRVADFALMYGIQAASPSILITGLFAVIVLSAVMSTTDRL
HHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCE
MLTVGTTFSWDIFKTMLKPMASERQVVLVSQICVLLAAVISLILAINPPEMLAFLIWLGI
EEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GLMLSAFAVPMIAGLYWRRATAAGAIASMLVGLITCLAFGAYYNFIDKLPMHFSIYSLLC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SIAAMVIVSLLTKKNSEKALDLTYTGMYLHPKDEPHSSTEQSKE
HHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; pantothenate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + pantothenate [Periplasm] = Na (I) [Cytoplasm] + pantothenate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]