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The map label for this gene is ribH

Identifier: 124485222

GI number: 124485222

Start: 372372

End: 372839

Strand: Reverse

Name: ribH

Synonym: Mlab_0395

Alternate gene names: 124485222

Gene position: 372839-372372 (Counterclockwise)

Preceding gene: 124485223

Following gene: 124485221

Centisome position: 20.66

GC content: 48.08

Gene sequence:

>468_bases
ATGAAAGTTTACGAAGGAAATCTGGTTTCAAAAAACATCAAAATAGGTATCGTTGCTGCAAGATTCAATGAATTTATCGT
ATCGAAATTACTCGGAGGAGCTCTCGATGCTCTCAAAAGACATGATGTCGCAGATGACGATGTCGAAATTGCCTGGGTCC
CCGGAGCATTCGAGATCCCGCTGATCGCAGAAAAGATGGCAGAATCCAAAAAATACGATGCGGTCATCTGCCTTGGCGCC
GTAATCCGCGGATCAACCAGCCACTACGATTATGTCTGCAATGAAGTCACAAAAGGCATAGCAACGGTTTCGCTGAAATC
AGGCATCCCGGTCATGTTTGGCGTTCTGACAACGGACAATCTCGAGCAGGCGATCGAACGTGCCGGATCAAAGGTCGGAA
ACAAAGGTTTCGATGCCGCGGTCGGCGCGATCGAAATGGTCAATGTTATCCGTCAACTCGGCAACTGA

Upstream 100 bases:

>100_bases
ACCATCAAAGACCTCCAGGCCTACCGGAGAAAGCAGGAAGCTTCGGCTCTTGCCGTTGAACAGAAAAATATGAGTCCAAT
GGACTGTTAAGGAGATTATC

Downstream 100 bases:

>100_bases
TACTCCATACACTCCTTTTTTTCTATAGAATATTATTAAGATGCGTTGCGTGTAAGATAAGAACCAAGTATGATTTTGGA
GGAATCACCTCGATGTACGG

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MKVYEGNLVSKNIKIGIVAARFNEFIVSKLLGGALDALKRHDVADDDVEIAWVPGAFEIPLIAEKMAESKKYDAVICLGA
VIRGSTSHYDYVCNEVTKGIATVSLKSGIPVMFGVLTTDNLEQAIERAGSKVGNKGFDAAVGAIEMVNVIRQLGN

Sequences:

>Translated_155_residues
MKVYEGNLVSKNIKIGIVAARFNEFIVSKLLGGALDALKRHDVADDDVEIAWVPGAFEIPLIAEKMAESKKYDAVICLGA
VIRGSTSHYDYVCNEVTKGIATVSLKSGIPVMFGVLTTDNLEQAIERAGSKVGNKGFDAAVGAIEMVNVIRQLGN
>Mature_155_residues
MKVYEGNLVSKNIKIGIVAARFNEFIVSKLLGGALDALKRHDVADDDVEIAWVPGAFEIPLIAEKMAESKKYDAVICLGA
VIRGSTSHYDYVCNEVTKGIATVSLKSGIPVMFGVLTTDNLEQAIERAGSKVGNKGFDAAVGAIEMVNVIRQLGN

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=154, Percent_Identity=51.9480519480519, Blast_Score=168, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6324429, Length=154, Percent_Identity=33.1168831168831, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_METLZ (A2SQG5)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001029838.1
- ProteinModelPortal:   A2SQG5
- SMR:   A2SQG5
- STRING:   A2SQG5
- GeneID:   4794685
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_0395
- eggNOG:   arNOG09975
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- PhylomeDB:   A2SQG5
- ProtClustDB:   PRK00061
- BioCyc:   MLAB410358:MLAB_0395-MONOMER
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16584; Mature: 16584

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVYEGNLVSKNIKIGIVAARFNEFIVSKLLGGALDALKRHDVADDDVEIAWVPGAFEIP
CCEECCCEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCH
LIAEKMAESKKYDAVICLGAVIRGSTSHYDYVCNEVTKGIATVSLKSGIPVMFGVLTTDN
HHHHHHHHCCCCCHHEEEHHHHCCCCCHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCC
LEQAIERAGSKVGNKGFDAAVGAIEMVNVIRQLGN
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVYEGNLVSKNIKIGIVAARFNEFIVSKLLGGALDALKRHDVADDDVEIAWVPGAFEIP
CCEECCCEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCH
LIAEKMAESKKYDAVICLGAVIRGSTSHYDYVCNEVTKGIATVSLKSGIPVMFGVLTTDN
HHHHHHHHCCCCCHHEEEHHHHCCCCCHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCC
LEQAIERAGSKVGNKGFDAAVGAIEMVNVIRQLGN
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA