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The map label for this gene is cca [H]

Identifier: 124485196

GI number: 124485196

Start: 346807

End: 348201

Strand: Reverse

Name: cca [H]

Synonym: Mlab_0369

Alternate gene names: 124485196

Gene position: 348201-346807 (Counterclockwise)

Preceding gene: 124485197

Following gene: 124485195

Centisome position: 19.29

GC content: 49.68

Gene sequence:

>1395_bases
ATGAGAGATCCAATTGAGGAGGAAGTATTACAAAATATCCGGCCCACGGAAGAAGAGCGGCACGAAGTTTTTGAGATGGG
ACAAAAACTCATCGAAGCGGTGAGAGAAATCGCCGGTGTTCCGGCCATGATGACGGGATCCGTGGCCCGAGGAACATGGG
TAAGGGGCGACAAAGACATAGATATTTTCATGCTGTTTCCCCCGACACTGTCAAGGGAAGAACTTCAGGAAAAAGGACTT
GCCGCAGCATACGGCGTCGTGGCTAAATTCAACGGATCGTCAGAAGAAAAATACGCAGAACATCCCTACCTGAATGCCGT
CATCGAAGGTTTCGACGTGGACCTGGTCCCCTGCTATCACGTAGAATCCACCGCCGAGATGAAATGTGCCGTTGACCGGA
CCCCTTTCCATACGCGATATCTGCTTGATCGAATAGACGGACTTCAGGACGACGTGCTCCTTATGAAACAGTTTTCAAAA
GGAGGCGGCGTATACGGATCGGATCATATGACCGGAGGATTCTCCGGATATCTCTGCGAACTACTCGTCCTCGCATATGG
AGGATTCAACGCGCTGCTTCAGGCAGCTGCAAAATTCCGTTTCGGAGAGGTCATCGATATCGAGGGATTCTATCCCGACA
AAAAAACGATTCGAAAGCTCTTCTCCGAACCGTTAATCGTCATAGACCCAACCGATAAAACCAGAAACGTAGCGGCAGCA
TTGACCCCCACACGGTTCTCTGAATTCATGGAACTTGCCAGAGACTACTGCGCGAGACCCGATTCCTGCTACTTCATTCC
TGATTCCCCAACCTCGATTTCAAAGGAGGAGTTCGCAGAAACACTTGAGATGAGGGGGACAACAACGCTGGCATTATCAT
TCAAAACACCCGCATATGTTCCCGACACCGTCGTTCCTCAGTTAAGAAAAACGATGGAGTCCATCAAAAATCAGCTTACA
GCCGAAGACTTCCACGTTCATCGGGCGGAAGTGGCGATGGGGGCAGACCACTGTCTTATCCTCTTCGAACTGCTGGTCGC
CGACCTGCCGACGATAACGCTGAGAAACGGACCGCCTGTTTGGGTTCACGACAACGCAGACAAATTCGTGGACAAGTACC
TTCGAGAAAATCCCTTTGCGGGACCGTGGATCGAGGAAGACCGTTATTATACCGAGGTGGAACGCAAATACAGGCATGCA
TCCGATCTTCTGGCTGATAAGCAGATGATCCTCTCCGGATCGATTGGAAAACATGTCAGGATCTCGATGGAGGAAGAATA
CACTATACTTAAAAACGAAGATATATGGTCACCTGAATTCACCTTCTTCCTGTCGGCATACTTTGCAAAAAGCTCTCATG
CGGTCAGAAAACTCCGGGTACTTGAACACCAATGA

Upstream 100 bases:

>100_bases
ACGCCGAATTTGGTACATGTATGATCGAAGAAATCCTCCTAAAACAGAGCACATTAACCCCACGGGGATCCATATATACA
ACACTTGCCCGGGTGAAGTT

Downstream 100 bases:

>100_bases
CATTATCTGAGATCCTAAGAGAACATTCACCGGCTTTAGTAGCTCTCTCCGGCGGGACCGACAGTATGACCCTTCTCGCG
GCATTCGTCAAAGAGGGGGG

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MRDPIEEEVLQNIRPTEEERHEVFEMGQKLIEAVREIAGVPAMMTGSVARGTWVRGDKDIDIFMLFPPTLSREELQEKGL
AAAYGVVAKFNGSSEEKYAEHPYLNAVIEGFDVDLVPCYHVESTAEMKCAVDRTPFHTRYLLDRIDGLQDDVLLMKQFSK
GGGVYGSDHMTGGFSGYLCELLVLAYGGFNALLQAAAKFRFGEVIDIEGFYPDKKTIRKLFSEPLIVIDPTDKTRNVAAA
LTPTRFSEFMELARDYCARPDSCYFIPDSPTSISKEEFAETLEMRGTTTLALSFKTPAYVPDTVVPQLRKTMESIKNQLT
AEDFHVHRAEVAMGADHCLILFELLVADLPTITLRNGPPVWVHDNADKFVDKYLRENPFAGPWIEEDRYYTEVERKYRHA
SDLLADKQMILSGSIGKHVRISMEEEYTILKNEDIWSPEFTFFLSAYFAKSSHAVRKLRVLEHQ

Sequences:

>Translated_464_residues
MRDPIEEEVLQNIRPTEEERHEVFEMGQKLIEAVREIAGVPAMMTGSVARGTWVRGDKDIDIFMLFPPTLSREELQEKGL
AAAYGVVAKFNGSSEEKYAEHPYLNAVIEGFDVDLVPCYHVESTAEMKCAVDRTPFHTRYLLDRIDGLQDDVLLMKQFSK
GGGVYGSDHMTGGFSGYLCELLVLAYGGFNALLQAAAKFRFGEVIDIEGFYPDKKTIRKLFSEPLIVIDPTDKTRNVAAA
LTPTRFSEFMELARDYCARPDSCYFIPDSPTSISKEEFAETLEMRGTTTLALSFKTPAYVPDTVVPQLRKTMESIKNQLT
AEDFHVHRAEVAMGADHCLILFELLVADLPTITLRNGPPVWVHDNADKFVDKYLRENPFAGPWIEEDRYYTEVERKYRHA
SDLLADKQMILSGSIGKHVRISMEEEYTILKNEDIWSPEFTFFLSAYFAKSSHAVRKLRVLEHQ
>Mature_464_residues
MRDPIEEEVLQNIRPTEEERHEVFEMGQKLIEAVREIAGVPAMMTGSVARGTWVRGDKDIDIFMLFPPTLSREELQEKGL
AAAYGVVAKFNGSSEEKYAEHPYLNAVIEGFDVDLVPCYHVESTAEMKCAVDRTPFHTRYLLDRIDGLQDDVLLMKQFSK
GGGVYGSDHMTGGFSGYLCELLVLAYGGFNALLQAAAKFRFGEVIDIEGFYPDKKTIRKLFSEPLIVIDPTDKTRNVAAA
LTPTRFSEFMELARDYCARPDSCYFIPDSPTSISKEEFAETLEMRGTTTLALSFKTPAYVPDTVVPQLRKTMESIKNQLT
AEDFHVHRAEVAMGADHCLILFELLVADLPTITLRNGPPVWVHDNADKFVDKYLRENPFAGPWIEEDRYYTEVERKYRHA
SDLLADKQMILSGSIGKHVRISMEEEYTILKNEDIWSPEFTFFLSAYFAKSSHAVRKLRVLEHQ

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG1746

COG function: function code J; tRNA nucleotidyltransferase (CCA-adding enzyme)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008229
- InterPro:   IPR002934
- InterPro:   IPR011068
- InterPro:   IPR015329 [H]

Pfam domain/function: PF01909 NTP_transf_2; PF09249 tRNA_NucTransf2 [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 52705; Mature: 52705

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDPIEEEVLQNIRPTEEERHEVFEMGQKLIEAVREIAGVPAMMTGSVARGTWVRGDKDI
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCE
DIFMLFPPTLSREELQEKGLAAAYGVVAKFNGSSEEKYAEHPYLNAVIEGFDVDLVPCYH
EEEEECCCCCCHHHHHHCCCHHHHHHEEEECCCCCHHHHCCCHHHHHHHCCCCCEEEEEE
VESTAEMKCAVDRTPFHTRYLLDRIDGLQDDVLLMKQFSKGGGVYGSDHMTGGFSGYLCE
CCCCCHHEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
LLVLAYGGFNALLQAAAKFRFGEVIDIEGFYPDKKTIRKLFSEPLIVIDPTDKTRNVAAA
HHHHHHCCHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCEEEECCCCCCCCEEEE
LTPTRFSEFMELARDYCARPDSCYFIPDSPTSISKEEFAETLEMRGTTTLALSFKTPAYV
ECHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCC
PDTVVPQLRKTMESIKNQLTAEDFHVHRAEVAMGADHCLILFELLVADLPTITLRNGPPV
CCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCE
WVHDNADKFVDKYLRENPFAGPWIEEDRYYTEVERKYRHASDLLADKQMILSGSIGKHVR
EEECCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
ISMEEEYTILKNEDIWSPEFTFFLSAYFAKSSHAVRKLRVLEHQ
EEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRDPIEEEVLQNIRPTEEERHEVFEMGQKLIEAVREIAGVPAMMTGSVARGTWVRGDKDI
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCE
DIFMLFPPTLSREELQEKGLAAAYGVVAKFNGSSEEKYAEHPYLNAVIEGFDVDLVPCYH
EEEEECCCCCCHHHHHHCCCHHHHHHEEEECCCCCHHHHCCCHHHHHHHCCCCCEEEEEE
VESTAEMKCAVDRTPFHTRYLLDRIDGLQDDVLLMKQFSKGGGVYGSDHMTGGFSGYLCE
CCCCCHHEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
LLVLAYGGFNALLQAAAKFRFGEVIDIEGFYPDKKTIRKLFSEPLIVIDPTDKTRNVAAA
HHHHHHCCHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCEEEECCCCCCCCEEEE
LTPTRFSEFMELARDYCARPDSCYFIPDSPTSISKEEFAETLEMRGTTTLALSFKTPAYV
ECHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCC
PDTVVPQLRKTMESIKNQLTAEDFHVHRAEVAMGADHCLILFELLVADLPTITLRNGPPV
CCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCE
WVHDNADKFVDKYLRENPFAGPWIEEDRYYTEVERKYRHASDLLADKQMILSGSIGKHVR
EEECCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
ISMEEEYTILKNEDIWSPEFTFFLSAYFAKSSHAVRKLRVLEHQ
EEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA