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The map label for this gene is pyrE

Identifier: 124485144

GI number: 124485144

Start: 301418

End: 301927

Strand: Reverse

Name: pyrE

Synonym: Mlab_0317

Alternate gene names: 124485144

Gene position: 301927-301418 (Counterclockwise)

Preceding gene: 124485145

Following gene: 124485143

Centisome position: 16.73

GC content: 53.92

Gene sequence:

>510_bases
ATGGTAAACAGAATATTAGACCTTCTGATTCAATATAAGGCAGTGGAATTCGGGGATTTCACCCTCGCGTCCGGTGCTCA
AAGCAAATACTACATCGATGTAAAAACGGCGATCATGCAGCCGGAACTCCTCTCCGAGATTGCAGCTGAAGTCGCGAAAA
AATACGATTTCGAGTGTATCGCGGGCGTCGCCGTTGGAGGGGTCCCGCTCGCCGTGGCCGTATCTCTGGCAGCAAATAAA
CCGTGCGCCGTCATCCGGGCAGCAGCAAAAGACCATGGAAAAAGTCAGATGATCATCGGAAACGTAAAAGGACAACGGGT
CCTGTTAATCGAAGACGTCACGACGTCGGGCGGTTCATCAAAGTACGGCGTCGAAGAACTGCGGAAAGCCGGAGCGCTGA
TCGATTCCGTCGTCACCGTCGTTGACCGGGAAGGCGGGGCAGAAGAACTTCTCGCCGCAGAAGGGATCACGCTGCACCCG
CTCGTGAAAGCGAGCGAACTTCTCGCATAA

Upstream 100 bases:

>100_bases
GATTCCTGATCGCGACCATTATTGCCATCCTCATACTATCACCTCTATTACATAGAGGAACAAATATTATCGGATACTTA
CTCGGGTTAAAGGACGTACC

Downstream 100 bases:

>100_bases
TTTTCTCATTTTCGCGCCTCTAAAAGATCATTTCTTTCCGGTTTATTTGAGAAACCAGAAACAATCTTTATTCAGAGATG
GTCGAATTACAAAGTCGGCA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MVNRILDLLIQYKAVEFGDFTLASGAQSKYYIDVKTAIMQPELLSEIAAEVAKKYDFECIAGVAVGGVPLAVAVSLAANK
PCAVIRAAAKDHGKSQMIIGNVKGQRVLLIEDVTTSGGSSKYGVEELRKAGALIDSVVTVVDREGGAEELLAAEGITLHP
LVKASELLA

Sequences:

>Translated_169_residues
MVNRILDLLIQYKAVEFGDFTLASGAQSKYYIDVKTAIMQPELLSEIAAEVAKKYDFECIAGVAVGGVPLAVAVSLAANK
PCAVIRAAAKDHGKSQMIIGNVKGQRVLLIEDVTTSGGSSKYGVEELRKAGALIDSVVTVVDREGGAEELLAAEGITLHP
LVKASELLA
>Mature_169_residues
MVNRILDLLIQYKAVEFGDFTLASGAQSKYYIDVKTAIMQPELLSEIAAEVAKKYDFECIAGVAVGGVPLAVAVSLAANK
PCAVIRAAAKDHGKSQMIIGNVKGQRVLLIEDVTTSGGSSKYGVEELRKAGALIDSVVTVVDREGGAEELLAAEGITLHP
LVKASELLA

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=166, Percent_Identity=34.9397590361446, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1790073, Length=152, Percent_Identity=29.6052631578947, Blast_Score=67, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17508631, Length=167, Percent_Identity=32.9341317365269, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17554964, Length=172, Percent_Identity=31.3953488372093, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323530, Length=156, Percent_Identity=32.0512820512821, Blast_Score=65, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323927, Length=155, Percent_Identity=31.6129032258064, Blast_Score=62, Evalue=5e-11,
Organism=Drosophila melanogaster, GI17933654, Length=166, Percent_Identity=31.3253012048193, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_METLZ (A2SQ87)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001029760.1
- ProteinModelPortal:   A2SQ87
- SMR:   A2SQ87
- STRING:   A2SQ87
- GeneID:   4795219
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_0317
- eggNOG:   arNOG09150
- HOGENOM:   HBG404341
- OMA:   GVIERTK
- PhylomeDB:   A2SQ87
- ProtClustDB:   PRK13811
- BioCyc:   MLAB410358:MLAB_0317-MONOMER
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 17757; Mature: 17757

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 86-86 BINDING 90-90 BINDING 92-92 BINDING 115-115 BINDING 143-143

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNRILDLLIQYKAVEFGDFTLASGAQSKYYIDVKTAIMQPELLSEIAAEVAKKYDFECI
CHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHCCCHHH
AGVAVGGVPLAVAVSLAANKPCAVIRAAAKDHGKSQMIIGNVKGQRVLLIEDVTTSGGSS
HHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCC
KYGVEELRKAGALIDSVVTVVDREGGAEELLAAEGITLHPLVKASELLA
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEECHHHHHHHHCC
>Mature Secondary Structure
MVNRILDLLIQYKAVEFGDFTLASGAQSKYYIDVKTAIMQPELLSEIAAEVAKKYDFECI
CHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHCCCHHH
AGVAVGGVPLAVAVSLAANKPCAVIRAAAKDHGKSQMIIGNVKGQRVLLIEDVTTSGGSS
HHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCC
KYGVEELRKAGALIDSVVTVVDREGGAEELLAAEGITLHPLVKASELLA
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA