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The map label for this gene is purD

Identifier: 124485143

GI number: 124485143

Start: 299915

End: 301207

Strand: Reverse

Name: purD

Synonym: Mlab_0316

Alternate gene names: 124485143

Gene position: 301207-299915 (Counterclockwise)

Preceding gene: 124485144

Following gene: 124485142

Centisome position: 16.69

GC content: 53.44

Gene sequence:

>1293_bases
ATGAAAATATTAGTGGCCGGAGGTGGCGGAAGGGAACACGCCATCTGCTTAGCACTCACCAAAAATGCGAACGTTGAACT
CTACTCAGTCATGGGGAAAAAGAATCCGGGCATCGCAAAAATAGCCCGCGAATCCTTCATCCACGCAGAAACGGACGTAC
CCGCAGTTCTTGCATTCGCAAAAGAACACAATATCCAATATGCCGTTGTCGGACCCGAAGCCCCGCTCGAAGCAGGTCTC
GTCGATGCCCTTACAAAAGCAGGAATCGGGTGTGTGGGCCCGGTCAAAGCTGCTGCACGCATCGAAACCGACAAGGGATT
TTGCCGCGGCCTCATGAATAAATACGGAATCGACGGCTGCCCGGCATACAAACTCTGCAATACCCCCAAAGAAGCCGCCG
AATTCATTCGCGAATATCCAGGCGATCTTGCAGTCAAACCGACCGGCCTTACCGGCGGAAAAGGGGTCAAAGTCATGGGC
GAACAGGTCGACCGCGAAGGGGCCGTCGAATACGCCATGACCTTGAAAGATCAGGTCATCATCCTCGAAGAACGCCTTCT
CGGCGAAGAGTTCACGTTGATGGCATTCGTCGACGGCAAACATCTCGTTCCCATGCCGCTCGTTCAGGATCACAAACGCG
CCTTTGAAGGAGATGTCGGCCCCAACACCGGCGGGATGGGATCCTACTCGCTCGAAAACCACAAGTTCCCCTTTGTTACC
GAAGACGACTATGCTAAGGCGATCTCCATCATGCAGGCGACCATCGACGCGCTCGCACAAGAGGGAAGTCCTTACAAAGG
TATTCTCTACGGCCAATTTATGAACACAAAGACCGGGCCGAAAGTGATCGAGTTCAATGCACGGTTCGGTGACCCCGAAG
CGATGAACGTGTTGTCGATCCTGACCTCGGATTTCCTGACCATCGCAGAACACATCATCAACGGCACGCTTTCGGCAAAC
GACGTCTCCTTTGAGGAGAAAGCCACGGTCTGCAAATATATCGTGCCGATGGACTACCCCGACAAACCTCATGCAGGCGA
TGTCATCACCGTCGGGCCGGCAGAGAATACCATTCTCTATTATGCAAACATCGCTTTGGAAAACGGTGTCCTGATGACCC
TGACCTCCCGCACGATGGCATACGTTGGGATCGGGGCCTCGCTTGCTGAAGCGGAAAAATATGCGGAAGCAGCCTGCAGG
AATGTATCCGGCAACGTCAGATACCGGAGTGATATCGGAACAGAGGCGCTGTTTGCAAAACGCATCGCCCACATGAAGGA
ATTAAGATCATGA

Upstream 100 bases:

>100_bases
AGAAAGGTTCGTATTTTTTCAGTCAGTGAAGTGATATGAAGCCGCAAATACCAAGTTATTGGAAACATTATTTGTCTGTT
AACGCGCCATAGATAAGGAT

Downstream 100 bases:

>100_bases
AAAAAGATTTTCTTTCGATCACCGACCTCTCGGCAGAGGAGTATGAAGACATTCTCACCCTGGCTGCCCGACTCAAACGC
CAGCGGTATGCCGGCGTCCC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKILVAGGGGREHAICLALTKNANVELYSVMGKKNPGIAKIARESFIHAETDVPAVLAFAKEHNIQYAVVGPEAPLEAGL
VDALTKAGIGCVGPVKAAARIETDKGFCRGLMNKYGIDGCPAYKLCNTPKEAAEFIREYPGDLAVKPTGLTGGKGVKVMG
EQVDREGAVEYAMTLKDQVIILEERLLGEEFTLMAFVDGKHLVPMPLVQDHKRAFEGDVGPNTGGMGSYSLENHKFPFVT
EDDYAKAISIMQATIDALAQEGSPYKGILYGQFMNTKTGPKVIEFNARFGDPEAMNVLSILTSDFLTIAEHIINGTLSAN
DVSFEEKATVCKYIVPMDYPDKPHAGDVITVGPAENTILYYANIALENGVLMTLTSRTMAYVGIGASLAEAEKYAEAACR
NVSGNVRYRSDIGTEALFAKRIAHMKELRS

Sequences:

>Translated_430_residues
MKILVAGGGGREHAICLALTKNANVELYSVMGKKNPGIAKIARESFIHAETDVPAVLAFAKEHNIQYAVVGPEAPLEAGL
VDALTKAGIGCVGPVKAAARIETDKGFCRGLMNKYGIDGCPAYKLCNTPKEAAEFIREYPGDLAVKPTGLTGGKGVKVMG
EQVDREGAVEYAMTLKDQVIILEERLLGEEFTLMAFVDGKHLVPMPLVQDHKRAFEGDVGPNTGGMGSYSLENHKFPFVT
EDDYAKAISIMQATIDALAQEGSPYKGILYGQFMNTKTGPKVIEFNARFGDPEAMNVLSILTSDFLTIAEHIINGTLSAN
DVSFEEKATVCKYIVPMDYPDKPHAGDVITVGPAENTILYYANIALENGVLMTLTSRTMAYVGIGASLAEAEKYAEAACR
NVSGNVRYRSDIGTEALFAKRIAHMKELRS
>Mature_430_residues
MKILVAGGGGREHAICLALTKNANVELYSVMGKKNPGIAKIARESFIHAETDVPAVLAFAKEHNIQYAVVGPEAPLEAGL
VDALTKAGIGCVGPVKAAARIETDKGFCRGLMNKYGIDGCPAYKLCNTPKEAAEFIREYPGDLAVKPTGLTGGKGVKVMG
EQVDREGAVEYAMTLKDQVIILEERLLGEEFTLMAFVDGKHLVPMPLVQDHKRAFEGDVGPNTGGMGSYSLENHKFPFVT
EDDYAKAISIMQATIDALAQEGSPYKGILYGQFMNTKTGPKVIEFNARFGDPEAMNVLSILTSDFLTIAEHIINGTLSAN
DVSFEEKATVCKYIVPMDYPDKPHAGDVITVGPAENTILYYANIALENGVLMTLTSRTMAYVGIGASLAEAEKYAEAACR
NVSGNVRYRSDIGTEALFAKRIAHMKELRS

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI4503915, Length=446, Percent_Identity=37.219730941704, Blast_Score=249, Evalue=4e-66,
Organism=Homo sapiens, GI209869995, Length=446, Percent_Identity=37.219730941704, Blast_Score=249, Evalue=4e-66,
Organism=Homo sapiens, GI209869993, Length=446, Percent_Identity=37.219730941704, Blast_Score=249, Evalue=4e-66,
Organism=Homo sapiens, GI28416899, Length=433, Percent_Identity=37.4133949191686, Blast_Score=248, Evalue=9e-66,
Organism=Escherichia coli, GI1790438, Length=433, Percent_Identity=34.6420323325635, Blast_Score=218, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI17567511, Length=431, Percent_Identity=30.8584686774942, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321203, Length=449, Percent_Identity=30.0668151447661, Blast_Score=169, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24582400, Length=438, Percent_Identity=34.9315068493151, Blast_Score=201, Evalue=8e-52,
Organism=Drosophila melanogaster, GI62471619, Length=438, Percent_Identity=34.9315068493151, Blast_Score=201, Evalue=9e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR2_METLZ (A2SQ86)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001029759.1
- STRING:   A2SQ86
- GeneID:   4795228
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_0316
- eggNOG:   arNOG04186
- HOGENOM:   HBG404060
- OMA:   GVPIVIK
- ProtClustDB:   PRK00885
- BioCyc:   MLAB410358:MLAB_0316-MONOMER
- HAMAP:   MF_00138
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.600.10
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR00877

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.13

Molecular weight: Translated: 46327; Mature: 46327

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50975 ATP_GRASP; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVAGGGGREHAICLALTKNANVELYSVMGKKNPGIAKIARESFIHAETDVPAVLAFA
CEEEEECCCCCCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHH
KEHNIQYAVVGPEAPLEAGLVDALTKAGIGCVGPVKAAARIETDKGFCRGLMNKYGIDGC
HHCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHEEECCHHHHHHHHHHCCCCCC
PAYKLCNTPKEAAEFIREYPGDLAVKPTGLTGGKGVKVMGEQVDREGAVEYAMTLKDQVI
CCHHHCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCEEHHHHCCCCHHHHHEEECCCEEE
ILEERLLGEEFTLMAFVDGKHLVPMPLVQDHKRAFEGDVGPNTGGMGSYSLENHKFPFVT
EEEHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
EDDYAKAISIMQATIDALAQEGSPYKGILYGQFMNTKTGPKVIEFNARFGDPEAMNVLSI
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHH
LTSDFLTIAEHIINGTLSANDVSFEEKATVCKYIVPMDYPDKPHAGDVITVGPAENTILY
HHHHHHHHHHHHHCCCCCCCCCCCHHHHCCEEEEECCCCCCCCCCCCEEEECCCCCEEEE
YANIALENGVLMTLTSRTMAYVGIGASLAEAEKYAEAACRNVSGNVRYRSDIGTEALFAK
EEEEEECCCEEEEEECCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHH
RIAHMKELRS
HHHHHHHHCC
>Mature Secondary Structure
MKILVAGGGGREHAICLALTKNANVELYSVMGKKNPGIAKIARESFIHAETDVPAVLAFA
CEEEEECCCCCCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHH
KEHNIQYAVVGPEAPLEAGLVDALTKAGIGCVGPVKAAARIETDKGFCRGLMNKYGIDGC
HHCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHEEECCHHHHHHHHHHCCCCCC
PAYKLCNTPKEAAEFIREYPGDLAVKPTGLTGGKGVKVMGEQVDREGAVEYAMTLKDQVI
CCHHHCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCEEHHHHCCCCHHHHHEEECCCEEE
ILEERLLGEEFTLMAFVDGKHLVPMPLVQDHKRAFEGDVGPNTGGMGSYSLENHKFPFVT
EEEHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
EDDYAKAISIMQATIDALAQEGSPYKGILYGQFMNTKTGPKVIEFNARFGDPEAMNVLSI
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHH
LTSDFLTIAEHIINGTLSANDVSFEEKATVCKYIVPMDYPDKPHAGDVITVGPAENTILY
HHHHHHHHHHHHHCCCCCCCCCCCHHHHCCEEEEECCCCCCCCCCCCEEEECCCCCEEEE
YANIALENGVLMTLTSRTMAYVGIGASLAEAEKYAEAACRNVSGNVRYRSDIGTEALFAK
EEEEEECCCEEEEEECCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHH
RIAHMKELRS
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA