Click here to switch to the map view.

The map label for this gene is argF

Identifier: 124485142

GI number: 124485142

Start: 299001

End: 299918

Strand: Reverse

Name: argF

Synonym: Mlab_0315

Alternate gene names: 124485142

Gene position: 299918-299001 (Counterclockwise)

Preceding gene: 124485143

Following gene: 124485141

Centisome position: 16.62

GC content: 55.45

Gene sequence:

>918_bases
ATGAAAAAAGATTTTCTTTCGATCACCGACCTCTCGGCAGAGGAGTATGAAGACATTCTCACCCTGGCTGCCCGACTCAA
ACGCCAGCGGTATGCCGGCGTCCCCCACCCGCTTCTCGCCGGAAAAACCCTCGCAATGATCTTCGAAAAGGCATCCACCA
GAACGCGGATGTCGTTTGACGTCGGCATGTACGATCTCGGCGGATATGCACTCTACCTGAATGCTAAAGACACACAGCTC
GGACGCGGGGAAACCGTCGCCGATACGGCACGGGTGATGTCCAGATACGTACACGGAGCGATCATGCGGACCTACAAACA
CGAGACGATCACCGAGTTCGCCAAATACGCATCCATCCCGGTGATCAACGCCCTTTCCGATAAAGAGCATCCCTGCCAAA
TCATGGCAGACTCATTAACCCTCAAAGAAAAATTCGGAGAGCTGGACGGGCTGAAGATCGCCTGGATCGGCGACGGGAAT
AATGTCTGTAATTCGCTGATAATGGCCTCCGTGCAAACCGGCATGGAAATCGCTGTCGGAACCCCGAAAGGATACGAACC
CGATCCGGCTGCCGTGAAGTTCGCCAAAGAAAACGGCGGGAAAGTCACCATCTACGATGATCCGGTCAGAGCCGTCAGCG
ATGCCCACGCAATTTACACCGACACCTGGATCTCGATGGGCGAAGAAGACATCAAAGAAACCAAGCTCAAAGACTTCGTC
GGCTACCAGCTCGACACCGCCCTGTTAAACAAAGCGGCGGACGACGCTCTCGTTCTCCACTGTCTTCCGGCCCACCGGGG
CGAGGAGATCACGGACGAAGTCATCGATTCCATGCAGAGCGGGGTCTGGGATCAGGCGGAGAACCGGCTGCATGCCCAGA
AAGCCATCCTCGTTCGGCTGATGACCCAAGGATACTAA

Upstream 100 bases:

>100_bases
CAGCCTGCAGGAATGTATCCGGCAACGTCAGATACCGGAGTGATATCGGAACAGAGGCGCTGTTTGCAAAACGCATCGCC
CACATGAAGGAATTAAGATC

Downstream 100 bases:

>100_bases
AATGCAGCCCGACTTTCCCCGAATCGGCAAACGGTTGTTTGCGGAACACTTGGTTGGAGGAAACTTCGGCAACATGAGTG
TAAGAACCGATGATGGATAT

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKKDFLSITDLSAEEYEDILTLAARLKRQRYAGVPHPLLAGKTLAMIFEKASTRTRMSFDVGMYDLGGYALYLNAKDTQL
GRGETVADTARVMSRYVHGAIMRTYKHETITEFAKYASIPVINALSDKEHPCQIMADSLTLKEKFGELDGLKIAWIGDGN
NVCNSLIMASVQTGMEIAVGTPKGYEPDPAAVKFAKENGGKVTIYDDPVRAVSDAHAIYTDTWISMGEEDIKETKLKDFV
GYQLDTALLNKAADDALVLHCLPAHRGEEITDEVIDSMQSGVWDQAENRLHAQKAILVRLMTQGY

Sequences:

>Translated_305_residues
MKKDFLSITDLSAEEYEDILTLAARLKRQRYAGVPHPLLAGKTLAMIFEKASTRTRMSFDVGMYDLGGYALYLNAKDTQL
GRGETVADTARVMSRYVHGAIMRTYKHETITEFAKYASIPVINALSDKEHPCQIMADSLTLKEKFGELDGLKIAWIGDGN
NVCNSLIMASVQTGMEIAVGTPKGYEPDPAAVKFAKENGGKVTIYDDPVRAVSDAHAIYTDTWISMGEEDIKETKLKDFV
GYQLDTALLNKAADDALVLHCLPAHRGEEITDEVIDSMQSGVWDQAENRLHAQKAILVRLMTQGY
>Mature_305_residues
MKKDFLSITDLSAEEYEDILTLAARLKRQRYAGVPHPLLAGKTLAMIFEKASTRTRMSFDVGMYDLGGYALYLNAKDTQL
GRGETVADTARVMSRYVHGAIMRTYKHETITEFAKYASIPVINALSDKEHPCQIMADSLTLKEKFGELDGLKIAWIGDGN
NVCNSLIMASVQTGMEIAVGTPKGYEPDPAAVKFAKENGGKVTIYDDPVRAVSDAHAIYTDTWISMGEEDIKETKLKDFV
GYQLDTALLNKAADDALVLHCLPAHRGEEITDEVIDSMQSGVWDQAENRLHAQKAILVRLMTQGY

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=305, Percent_Identity=45.2459016393443, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI18105007, Length=305, Percent_Identity=25.9016393442623, Blast_Score=101, Evalue=9e-22,
Organism=Escherichia coli, GI1790703, Length=328, Percent_Identity=36.890243902439, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI1786469, Length=324, Percent_Identity=36.4197530864198, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI2367364, Length=314, Percent_Identity=29.6178343949045, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI48994908, Length=318, Percent_Identity=26.4150943396226, Blast_Score=85, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI193204318, Length=328, Percent_Identity=25.609756097561, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322373, Length=309, Percent_Identity=38.8349514563107, Blast_Score=212, Evalue=7e-56,
Organism=Saccharomyces cerevisiae, GI6322331, Length=310, Percent_Identity=25.1612903225806, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24642586, Length=301, Percent_Identity=27.906976744186, Blast_Score=104, Evalue=8e-23,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_METLZ (A2SQ85)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001029758.1
- ProteinModelPortal:   A2SQ85
- SMR:   A2SQ85
- STRING:   A2SQ85
- GeneID:   4795229
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_0315
- eggNOG:   arNOG04256
- HOGENOM:   HBG579429
- OMA:   CVVTDTW
- PhylomeDB:   A2SQ85
- ProtClustDB:   PRK00779
- BioCyc:   MLAB410358:MLAB_0315-MONOMER
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 33762; Mature: 33762

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 103-103 BINDING 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKDFLSITDLSAEEYEDILTLAARLKRQRYAGVPHPLLAGKTLAMIFEKASTRTRMSFD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCHHHHHHHHHHCCCEEEEEE
VGMYDLGGYALYLNAKDTQLGRGETVADTARVMSRYVHGAIMRTYKHETITEFAKYASIP
CCEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VINALSDKEHPCQIMADSLTLKEKFGELDGLKIAWIGDGNNVCNSLIMASVQTGMEIAVG
EEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCEEEEC
TPKGYEPDPAAVKFAKENGGKVTIYDDPVRAVSDAHAIYTDTWISMGEEDIKETKLKDFV
CCCCCCCCHHHEEEEECCCCEEEEECCCHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHH
GYQLDTALLNKAADDALVLHCLPAHRGEEITDEVIDSMQSGVWDQAENRLHAQKAILVRL
CHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
MTQGY
HHCCC
>Mature Secondary Structure
MKKDFLSITDLSAEEYEDILTLAARLKRQRYAGVPHPLLAGKTLAMIFEKASTRTRMSFD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCHHHHHHHHHHCCCEEEEEE
VGMYDLGGYALYLNAKDTQLGRGETVADTARVMSRYVHGAIMRTYKHETITEFAKYASIP
CCEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VINALSDKEHPCQIMADSLTLKEKFGELDGLKIAWIGDGNNVCNSLIMASVQTGMEIAVG
EEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCEEEEC
TPKGYEPDPAAVKFAKENGGKVTIYDDPVRAVSDAHAIYTDTWISMGEEDIKETKLKDFV
CCCCCCCCHHHEEEEECCCCEEEEECCCHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHH
GYQLDTALLNKAADDALVLHCLPAHRGEEITDEVIDSMQSGVWDQAENRLHAQKAILVRL
CHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
MTQGY
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA