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The map label for this gene is def [H]

Identifier: 124485039

GI number: 124485039

Start: 202198

End: 202671

Strand: Reverse

Name: def [H]

Synonym: Mlab_0212

Alternate gene names: 124485039

Gene position: 202671-202198 (Counterclockwise)

Preceding gene: 124485042

Following gene: 124485038

Centisome position: 11.23

GC content: 48.31

Gene sequence:

>474_bases
ATGATCTGCGAGATACGAAAATACGGAGACCCTGTCCTCTTTTTGCATGCGGAAACGGTGCAAAACATCGGCCCGCTGGA
ATTAGAGATCCTGACGAACATGTGGGACACAATGATCCACAACAAATGCATTGGTCTTTCAGCTCCCCAGATAGGCGTAT
CGAAACGCCTCTTCATTGTAAATGCCGGAGGGGTAACCATCAAAGGAGCGAATCCGGAAGTATTGAAAGAAGGGGCACTT
GTTGAAGAAATGGAGGGAAGTCCCTGTATTCCAGGGATCCAGCGGCCGGTCCGCCGCCCTGGAAAGATCACCTGCAGATA
CCTCGATATTTCCGGTGAGACGATCGAGACCGAACTCAAAGGAATTGCTGCCCGGGCTTTTCTGCATGAAAAAGATCATC
ATGAAGGGATACTCTATCTTGATCATCTGAAACCAATCCAGAAACGCATGATCCTCAAATCCCTGGAAAAATAA

Upstream 100 bases:

>100_bases
TACGGTTCCAAATGCATGTCCTGGACGATCATACTGTAGATCCATGCATATGTATGTGTAATCACAACACTAAAACAATT
CCAGCGATAATACAAAATCT

Downstream 100 bases:

>100_bases
CGGAGATATATTTTCCATAAACCAAACCCTGATAACTTCTGAAAAGGAATATTTGATAATGAATCTCATTCCAACACGCG
AGGAAATAAAGGAAGTCGCT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MICEIRKYGDPVLFLHAETVQNIGPLELEILTNMWDTMIHNKCIGLSAPQIGVSKRLFIVNAGGVTIKGANPEVLKEGAL
VEEMEGSPCIPGIQRPVRRPGKITCRYLDISGETIETELKGIAARAFLHEKDHHEGILYLDHLKPIQKRMILKSLEK

Sequences:

>Translated_157_residues
MICEIRKYGDPVLFLHAETVQNIGPLELEILTNMWDTMIHNKCIGLSAPQIGVSKRLFIVNAGGVTIKGANPEVLKEGAL
VEEMEGSPCIPGIQRPVRRPGKITCRYLDISGETIETELKGIAARAFLHEKDHHEGILYLDHLKPIQKRMILKSLEK
>Mature_157_residues
MICEIRKYGDPVLFLHAETVQNIGPLELEILTNMWDTMIHNKCIGLSAPQIGVSKRLFIVNAGGVTIKGANPEVLKEGAL
VEEMEGSPCIPGIQRPVRRPGKITCRYLDISGETIETELKGIAARAFLHEKDHHEGILYLDHLKPIQKRMILKSLEK

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=156, Percent_Identity=30.1282051282051, Blast_Score=71, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 17548; Mature: 17548

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MICEIRKYGDPVLFLHAETVQNIGPLELEILTNMWDTMIHNKCIGLSAPQIGVSKRLFIV
CEECCCCCCCCEEEEEHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEE
NAGGVTIKGANPEVLKEGALVEEMEGSPCIPGIQRPVRRPGKITCRYLDISGETIETELK
ECCCEEEECCCHHHHHCCCHHHHCCCCCCCCCCHHHHCCCCCEEEEEEECCCCCHHHHHH
GIAARAFLHEKDHHEGILYLDHLKPIQKRMILKSLEK
HHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MICEIRKYGDPVLFLHAETVQNIGPLELEILTNMWDTMIHNKCIGLSAPQIGVSKRLFIV
CEECCCCCCCCEEEEEHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEE
NAGGVTIKGANPEVLKEGALVEEMEGSPCIPGIQRPVRRPGKITCRYLDISGETIETELK
ECCCEEEECCCHHHHHCCCHHHHCCCCCCCCCCHHHHCCCCCEEEEEEECCCCCHHHHHH
GIAARAFLHEKDHHEGILYLDHLKPIQKRMILKSLEK
HHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA