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The map label for this gene is appB [H]

Identifier: 124484998

GI number: 124484998

Start: 156798

End: 157799

Strand: Reverse

Name: appB [H]

Synonym: Mlab_0171

Alternate gene names: 124484998

Gene position: 157799-156798 (Counterclockwise)

Preceding gene: 124484999

Following gene: 124484997

Centisome position: 8.74

GC content: 45.11

Gene sequence:

>1002_bases
GTGAACTTGTCTGATTACCCATATCTGAAAAAAATTGGAAAAAACATCATTATATTCATAATAACGATGTTTGTACTTTC
CATAATCGTCTTTGTCATGGCACGTTTGGCTCCGGGAGACCCTTTGCAGGCATTTTACGGCGATGCGCTGGATACCATGA
GCATCGAAGAAATGGATGCAGCACGCGTAAGATTGGGTCTGGACGGGCCCATATATTATCAGTATATCAAATGGCTGCTC
CTCGCCCTTGGAGGGGACTTCGGTCTGTCGCTCCAGTATAAAATGCCGGCGATGGACGTCATTGCACCCCTTATCAGCAA
CACCATTATACTCGGACTCACTGCATACATTCTCGTTTTTGCCCTCGCAATTGTACTCGCCGTCATCTGTGCAAAACACG
AAGATACCCTGCTTGACAAACTAATCTGCAAAATCGGGACAATGAGCTATTTTATACCTGCCTTCTGGCTGGGTCTCATC
CTCATCCTCATATTCAGTGTCAACCTCAACTGGTTCCCAAGCAGCGGAGCATACGATTATGGTCAGGCGGGTAACATCGC
AAACAGAATCTGGCACATGATACTACCGCTCACCGTGATGATATTCTCCCATATCTGGTATTATGCATACATGATACGCA
ACAAGCTGCTGGATGAAAACAGAAAAGACTACGTCCTGCTTGCAAAAATCAAAGGTTTGACGAAATCCGAGATCCTCTGG
AAACATTCTCTCAGAAACATCGCACCGACAATTGTGAATCTCATGGCGATCTCGATTCCGCATGTATTAGGCGGGACCGT
GATCGCAGAAGCGGTATTTAACTACCCGGGAATAGGAAACATGGCAATAAGCGCTGCAAAATATCACGACTATAATCTCC
TCATGCTTCTTGTTCTCATCACGGGATCAATGGTGATCATAACAAGTATGTGTGCATATATGATCAACGAAGTCATAGAT
CCCCGTATGAAAGACGCAGGAGGTACAATATGGGAGAAATAG

Upstream 100 bases:

>100_bases
CACATGTAAGTATTACATTTTTTAAAATATGTAATATTTATAATTCATAAAAATAAATTAAGTAATACAATTTATTAAAT
TATATTGTCGGAGTGTAAAT

Downstream 100 bases:

>100_bases
ATCCTTCCGCATTTGAAATCGTTGGAGCAGAATACCAGACATGGGAACCTGAAATAAAAAAAGAATCTGTATCCAAAAAA
CTGAGAAAACTCCCATGGAT

Product: hypothetical protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MNLSDYPYLKKIGKNIIIFIITMFVLSIIVFVMARLAPGDPLQAFYGDALDTMSIEEMDAARVRLGLDGPIYYQYIKWLL
LALGGDFGLSLQYKMPAMDVIAPLISNTIILGLTAYILVFALAIVLAVICAKHEDTLLDKLICKIGTMSYFIPAFWLGLI
LILIFSVNLNWFPSSGAYDYGQAGNIANRIWHMILPLTVMIFSHIWYYAYMIRNKLLDENRKDYVLLAKIKGLTKSEILW
KHSLRNIAPTIVNLMAISIPHVLGGTVIAEAVFNYPGIGNMAISAAKYHDYNLLMLLVLITGSMVIITSMCAYMINEVID
PRMKDAGGTIWEK

Sequences:

>Translated_333_residues
MNLSDYPYLKKIGKNIIIFIITMFVLSIIVFVMARLAPGDPLQAFYGDALDTMSIEEMDAARVRLGLDGPIYYQYIKWLL
LALGGDFGLSLQYKMPAMDVIAPLISNTIILGLTAYILVFALAIVLAVICAKHEDTLLDKLICKIGTMSYFIPAFWLGLI
LILIFSVNLNWFPSSGAYDYGQAGNIANRIWHMILPLTVMIFSHIWYYAYMIRNKLLDENRKDYVLLAKIKGLTKSEILW
KHSLRNIAPTIVNLMAISIPHVLGGTVIAEAVFNYPGIGNMAISAAKYHDYNLLMLLVLITGSMVIITSMCAYMINEVID
PRMKDAGGTIWEK
>Mature_333_residues
MNLSDYPYLKKIGKNIIIFIITMFVLSIIVFVMARLAPGDPLQAFYGDALDTMSIEEMDAARVRLGLDGPIYYQYIKWLL
LALGGDFGLSLQYKMPAMDVIAPLISNTIILGLTAYILVFALAIVLAVICAKHEDTLLDKLICKIGTMSYFIPAFWLGLI
LILIFSVNLNWFPSSGAYDYGQAGNIANRIWHMILPLTVMIFSHIWYYAYMIRNKLLDENRKDYVLLAKIKGLTKSEILW
KHSLRNIAPTIVNLMAISIPHVLGGTVIAEAVFNYPGIGNMAISAAKYHDYNLLMLLVLITGSMVIITSMCAYMINEVID
PRMKDAGGTIWEK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=322, Percent_Identity=29.5031055900621, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI1787053, Length=311, Percent_Identity=29.5819935691318, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1789965, Length=337, Percent_Identity=26.7062314540059, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1787497, Length=317, Percent_Identity=28.0757097791798, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1787761, Length=322, Percent_Identity=25.776397515528, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788504, Length=292, Percent_Identity=27.3972602739726, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1787550, Length=272, Percent_Identity=25, Blast_Score=98, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 37351; Mature: 37351

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSDYPYLKKIGKNIIIFIITMFVLSIIVFVMARLAPGDPLQAFYGDALDTMSIEEMDA
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHCCCCHHHHHH
ARVRLGLDGPIYYQYIKWLLLALGGDFGLSLQYKMPAMDVIAPLISNTIILGLTAYILVF
HHHEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
ALAIVLAVICAKHEDTLLDKLICKIGTMSYFIPAFWLGLILILIFSVNLNWFPSSGAYDY
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
GQAGNIANRIWHMILPLTVMIFSHIWYYAYMIRNKLLDENRKDYVLLAKIKGLTKSEILW
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHH
KHSLRNIAPTIVNLMAISIPHVLGGTVIAEAVFNYPGIGNMAISAAKYHDYNLLMLLVLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHH
TGSMVIITSMCAYMINEVIDPRMKDAGGTIWEK
HCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCC
>Mature Secondary Structure
MNLSDYPYLKKIGKNIIIFIITMFVLSIIVFVMARLAPGDPLQAFYGDALDTMSIEEMDA
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHCCCCHHHHHH
ARVRLGLDGPIYYQYIKWLLLALGGDFGLSLQYKMPAMDVIAPLISNTIILGLTAYILVF
HHHEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
ALAIVLAVICAKHEDTLLDKLICKIGTMSYFIPAFWLGLILILIFSVNLNWFPSSGAYDY
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
GQAGNIANRIWHMILPLTVMIFSHIWYYAYMIRNKLLDENRKDYVLLAKIKGLTKSEILW
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHH
KHSLRNIAPTIVNLMAISIPHVLGGTVIAEAVFNYPGIGNMAISAAKYHDYNLLMLLVLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHH
TGSMVIITSMCAYMINEVIDPRMKDAGGTIWEK
HCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]