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The map label for this gene is nifH [H]

Identifier: 124484992

GI number: 124484992

Start: 150434

End: 151240

Strand: Reverse

Name: nifH [H]

Synonym: Mlab_0165

Alternate gene names: 124484992

Gene position: 151240-150434 (Counterclockwise)

Preceding gene: 124484993

Following gene: 124484991

Centisome position: 8.38

GC content: 51.55

Gene sequence:

>807_bases
ATGAGACAGATCGCCCTCTATGGAAAGGGAGGTATTGGAAAATCCACTACATCGGCAAATCTTTCGGCGGCATTTTCGGA
GATGGACCTGGATGTCATGCAGATCGGGTGTGACCCGAAACACGACAGCACACGGATGCTCATGCACGGACGCTGGATCC
CGACCGTTCTTGAGCAGATGTATGATCATAAAGAGATCAAAAGCGAGGACATCATCTATCAGGGATTTGGAAACATCCGC
TGTGTCGAGGCTGGAGGGCCGGAGCCCGGGATCGGGTGTGCAGGCAGAGGAATCATCGCAACATTCCAGCTTCTGGAAAA
GATGGATGCTCTCTACGGAGACGTTGTCGTATACGATGTTCTCGGGGACGTTGTCTGCGGAGGGTTTGCGATGCCGATGC
GTGACGGCTATGCGCAGGAAGTGTATCTTGTAACAAGCGGAGAGCTGATGAGTCTCTATGCTGCAAACAATATCTGCAAA
GCGATCGCGAGAATATCCGAACGAAGCACGGCAAAATGCCGGCTTGGTGGAGTGATCTGCAATGCAAAGAATATGGATAA
CGAACGCGAACTTGTCGAGGAGTTTGCAAAAAGGATCGGATCACGCCTTGTCTGTTATATTCCACGAAGCAAATCGGTCA
GAGCAGCGGAGGTGAACTGTATGACGGTCATCGAACACGATCCAACATCGGAACAGGCGGCCGTCTACCGGGACTGTGCC
AAAACCATTCTGGAAAACACCGATCTTCGGATTCCGACCCCGCTTGCATTAGAGGAACTGGAAGAGCTTGCAAGACACTA
TCTCTGA

Upstream 100 bases:

>100_bases
CTTCTTTTTTTCATCAAAGAGACGTATCGACTGGACCGTTCACCAGACAACATGGATTTAACTCAAATTATATGTATCCT
GCAGATCAATCTATAAGGGA

Downstream 100 bases:

>100_bases
ATCACGGATGGCCGGGTGCACTCTTACGGGGGCCCTTTCCGTCGCCTGTTTTATCCCGGGAGCAGTAACGGTAGTGCATG
CCCCCAAAGGCTGCGCCCAC

Product: nitrogenase iron protein subunit NifH

Products: NA

Alternate protein names: Nitrogenase Fe protein; Nitrogenase component II; Nitrogenase reductase [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MRQIALYGKGGIGKSTTSANLSAAFSEMDLDVMQIGCDPKHDSTRMLMHGRWIPTVLEQMYDHKEIKSEDIIYQGFGNIR
CVEAGGPEPGIGCAGRGIIATFQLLEKMDALYGDVVVYDVLGDVVCGGFAMPMRDGYAQEVYLVTSGELMSLYAANNICK
AIARISERSTAKCRLGGVICNAKNMDNERELVEEFAKRIGSRLVCYIPRSKSVRAAEVNCMTVIEHDPTSEQAAVYRDCA
KTILENTDLRIPTPLALEELEELARHYL

Sequences:

>Translated_268_residues
MRQIALYGKGGIGKSTTSANLSAAFSEMDLDVMQIGCDPKHDSTRMLMHGRWIPTVLEQMYDHKEIKSEDIIYQGFGNIR
CVEAGGPEPGIGCAGRGIIATFQLLEKMDALYGDVVVYDVLGDVVCGGFAMPMRDGYAQEVYLVTSGELMSLYAANNICK
AIARISERSTAKCRLGGVICNAKNMDNERELVEEFAKRIGSRLVCYIPRSKSVRAAEVNCMTVIEHDPTSEQAAVYRDCA
KTILENTDLRIPTPLALEELEELARHYL
>Mature_268_residues
MRQIALYGKGGIGKSTTSANLSAAFSEMDLDVMQIGCDPKHDSTRMLMHGRWIPTVLEQMYDHKEIKSEDIIYQGFGNIR
CVEAGGPEPGIGCAGRGIIATFQLLEKMDALYGDVVVYDVLGDVVCGGFAMPMRDGYAQEVYLVTSGELMSLYAANNICK
AIARISERSTAKCRLGGVICNAKNMDNERELVEEFAKRIGSRLVCYIPRSKSVRAAEVNCMTVIEHDPTSEQAAVYRDCA
KTILENTDLRIPTPLALEELEELARHYL

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein and the molybdenum-iron protein [H]

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000392
- InterPro:   IPR005977 [H]

Pfam domain/function: PF00142 Fer4_NifH [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 29572; Mature: 29572

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00746 NIFH_FRXC_1 ; PS00692 NIFH_FRXC_2 ; PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQIALYGKGGIGKSTTSANLSAAFSEMDLDVMQIGCDPKHDSTRMLMHGRWIPTVLEQM
CCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCCEEEECCCCHHHHHHHH
YDHKEIKSEDIIYQGFGNIRCVEAGGPEPGIGCAGRGIIATFQLLEKMDALYGDVVVYDV
HHHHHCCCCCEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LGDVVCGGFAMPMRDGYAQEVYLVTSGELMSLYAANNICKAIARISERSTAKCRLGGVIC
HHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEECEEEE
NAKNMDNERELVEEFAKRIGSRLVCYIPRSKSVRAAEVNCMTVIEHDPTSEQAAVYRDCA
CCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHH
KTILENTDLRIPTPLALEELEELARHYL
HHHHCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRQIALYGKGGIGKSTTSANLSAAFSEMDLDVMQIGCDPKHDSTRMLMHGRWIPTVLEQM
CCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCCEEEECCCCHHHHHHHH
YDHKEIKSEDIIYQGFGNIRCVEAGGPEPGIGCAGRGIIATFQLLEKMDALYGDVVVYDV
HHHHHCCCCCEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LGDVVCGGFAMPMRDGYAQEVYLVTSGELMSLYAANNICKAIARISERSTAKCRLGGVIC
HHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEECEEEE
NAKNMDNERELVEEFAKRIGSRLVCYIPRSKSVRAAEVNCMTVIEHDPTSEQAAVYRDCA
CCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHH
KTILENTDLRIPTPLALEELEELARHYL
HHHHCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA