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The map label for this gene is aspC

Identifier: 124484868

GI number: 124484868

Start: 41414

End: 42703

Strand: Reverse

Name: aspC

Synonym: Mlab_0039

Alternate gene names: 124484868

Gene position: 42703-41414 (Counterclockwise)

Preceding gene: 124484869

Following gene: 124484867

Centisome position: 2.37

GC content: 55.74

Gene sequence:

>1290_bases
ATGCGCATACCAATAAAAGACGTGACCCCTGAAATTGGGCATGCACGCATTGCAGGCTGGGTCCACGAAGAACGTGACCT
TGGAGGACTCACCTTCCTTCTCGTTCGCGACAGAACCGGGATCCTTCAGGTCACCATTCCGAAAAAGAAAGTCACCCCCG
AAGTACTCGCCGCCGTCAAAGAGGCGACCCGCGAGTCCGTCATCGAATGCGAAGGCGTTGTCAAAGCAACCGAAAAAGCA
CCCGGTGGACGCGAACTCGTCCCCGACATGCTCCGCGTCGTTTCCCGTGCCGAGACCCCTCTTCCGCTCGACGTCTCGGA
AAAAGTCCCCGCAGAACTCGACACCAGGCTCGACAACCGGTACCTCGATCTTAGAAAACCCCGCATCAACGCTATTTTCC
AGATAAGAAACGCCTGTCTTCGCGCCATCAGCGAATACCTCTGGGACAACGGATTCACCCAGGTCCAGACCCCAAAGATC
GTAGCTGCCGCAACTGAAGGAGGAACCGAACTCTTCCCGCTCGCCTACTTCGACAAAGAGGCATTCTTAAATCAGTCTCC
CCAGCTCTACAAACAGATGCTGATGAGCGCCGGGTTCGACAGGGTCTTCGAGATCGGTGCGATCTTCCGTGCAGAGGAAC
ACAACACCGTTCGTCACTTAAACGAAGCCACTTCGATCGATATCGAGATGTCCTTTGCAAATGAAGAGGACGCGATGCAT
GTACTCGAAAACGTCGTAGCTTCGGCATATCAATACGTCGCCGACCACTGCGGCGATGCTCTTGCAACGCTCGGCATCAC
CGATTTCAAGGTCCCGACCGTTCCGTTCCCGCGTATCACCTACAAAGAAGCAATCGAAATCTCCACCGCCGGCGGCGAAC
CGCTCGTTTTCGGCGACGATCTTTCGACCGCTGCCGAGAGGATCGTCGGAGAGAAGATGGGCACGATGTACTTCATCACC
GAATGGCCGACCTCCACGAGACCGTTCTACACCATGCCGTTCGAAGACCGCCCCGAGGTCTGTCGTGCATTCGATATGAT
GCACCCGAGAATGGAGCTGACTTCCGGCGCACAGCGTTGCCATATCCACTCACTTCTCGTCGAACAGATCAAAGCAAAAG
GTCTCAACCCCGACGCGTTCGAGTTCTACTTAAATCCGTTCAGATACGGTATGCCGCCCCACGCAGGCTGGGGACTTGGT
GCCGAACGCCTGGTCATGACCATGCTCGATCTCCAGAATATCAGGGAAGCCGTTCTCTTCCCGCGGGACCGACACAGAGT
CAGTCCATAA

Upstream 100 bases:

>100_bases
ACGAAGGGAAGTATGCCCATATCTCCTGGCGGGTGTGACCGAAGATTCCGACAACGGACAAACACTAATCTATCTCCACC
GCAAATACTATAGAGATTTT

Downstream 100 bases:

>100_bases
CCCTTTTTTTATGATAAAACATCTCGTTCTCTTCAAACTCAAAGACCCGGCATGTGCCGAAGAAACACGCAATATCCTCC
TTTCGATGAAAGGCGTTGTT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MRIPIKDVTPEIGHARIAGWVHEERDLGGLTFLLVRDRTGILQVTIPKKKVTPEVLAAVKEATRESVIECEGVVKATEKA
PGGRELVPDMLRVVSRAETPLPLDVSEKVPAELDTRLDNRYLDLRKPRINAIFQIRNACLRAISEYLWDNGFTQVQTPKI
VAAATEGGTELFPLAYFDKEAFLNQSPQLYKQMLMSAGFDRVFEIGAIFRAEEHNTVRHLNEATSIDIEMSFANEEDAMH
VLENVVASAYQYVADHCGDALATLGITDFKVPTVPFPRITYKEAIEISTAGGEPLVFGDDLSTAAERIVGEKMGTMYFIT
EWPTSTRPFYTMPFEDRPEVCRAFDMMHPRMELTSGAQRCHIHSLLVEQIKAKGLNPDAFEFYLNPFRYGMPPHAGWGLG
AERLVMTMLDLQNIREAVLFPRDRHRVSP

Sequences:

>Translated_429_residues
MRIPIKDVTPEIGHARIAGWVHEERDLGGLTFLLVRDRTGILQVTIPKKKVTPEVLAAVKEATRESVIECEGVVKATEKA
PGGRELVPDMLRVVSRAETPLPLDVSEKVPAELDTRLDNRYLDLRKPRINAIFQIRNACLRAISEYLWDNGFTQVQTPKI
VAAATEGGTELFPLAYFDKEAFLNQSPQLYKQMLMSAGFDRVFEIGAIFRAEEHNTVRHLNEATSIDIEMSFANEEDAMH
VLENVVASAYQYVADHCGDALATLGITDFKVPTVPFPRITYKEAIEISTAGGEPLVFGDDLSTAAERIVGEKMGTMYFIT
EWPTSTRPFYTMPFEDRPEVCRAFDMMHPRMELTSGAQRCHIHSLLVEQIKAKGLNPDAFEFYLNPFRYGMPPHAGWGLG
AERLVMTMLDLQNIREAVLFPRDRHRVSP
>Mature_429_residues
MRIPIKDVTPEIGHARIAGWVHEERDLGGLTFLLVRDRTGILQVTIPKKKVTPEVLAAVKEATRESVIECEGVVKATEKA
PGGRELVPDMLRVVSRAETPLPLDVSEKVPAELDTRLDNRYLDLRKPRINAIFQIRNACLRAISEYLWDNGFTQVQTPKI
VAAATEGGTELFPLAYFDKEAFLNQSPQLYKQMLMSAGFDRVFEIGAIFRAEEHNTVRHLNEATSIDIEMSFANEEDAMH
VLENVVASAYQYVADHCGDALATLGITDFKVPTVPFPRITYKEAIEISTAGGEPLVFGDDLSTAAERIVGEKMGTMYFIT
EWPTSTRPFYTMPFEDRPEVCRAFDMMHPRMELTSGAQRCHIHSLLVEQIKAKGLNPDAFEFYLNPFRYGMPPHAGWGLG
AERLVMTMLDLQNIREAVLFPRDRHRVSP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI45439306, Length=443, Percent_Identity=34.5372460496614, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI4758762, Length=425, Percent_Identity=29.4117647058824, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI226491502, Length=445, Percent_Identity=27.4157303370787, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI5031815, Length=465, Percent_Identity=27.3118279569892, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI194272210, Length=465, Percent_Identity=27.3118279569892, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI40789249, Length=277, Percent_Identity=30.3249097472924, Blast_Score=109, Evalue=6e-24,
Organism=Escherichia coli, GI1787161, Length=441, Percent_Identity=26.7573696145125, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1788173, Length=239, Percent_Identity=35.5648535564854, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1790571, Length=444, Percent_Identity=26.5765765765766, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789256, Length=448, Percent_Identity=25.8928571428571, Blast_Score=112, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17551876, Length=449, Percent_Identity=34.7438752783964, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI71984122, Length=425, Percent_Identity=32.2352941176471, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17556372, Length=379, Percent_Identity=26.6490765171504, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71994340, Length=456, Percent_Identity=24.5614035087719, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17535925, Length=456, Percent_Identity=24.5614035087719, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17535927, Length=456, Percent_Identity=24.5614035087719, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI32566633, Length=282, Percent_Identity=27.3049645390071, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323011, Length=446, Percent_Identity=36.322869955157, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6321807, Length=444, Percent_Identity=31.5315315315315, Blast_Score=223, Evalue=5e-59,
Organism=Saccharomyces cerevisiae, GI6319872, Length=482, Percent_Identity=26.1410788381743, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325153, Length=289, Percent_Identity=29.757785467128, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI17136276, Length=461, Percent_Identity=33.6225596529284, Blast_Score=271, Evalue=9e-73,
Organism=Drosophila melanogaster, GI19921528, Length=432, Percent_Identity=29.6296296296296, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI19922416, Length=445, Percent_Identity=25.3932584269663, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24584738, Length=302, Percent_Identity=31.1258278145695, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24640851, Length=462, Percent_Identity=25.3246753246753, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24640849, Length=462, Percent_Identity=25.3246753246753, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): SYD_METLZ (A2SPG1)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001029484.1
- ProteinModelPortal:   A2SPG1
- SMR:   A2SPG1
- STRING:   A2SPG1
- GeneID:   4795690
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_0039
- eggNOG:   arNOG04316
- HOGENOM:   HBG745843
- OMA:   ASAFPRD
- PhylomeDB:   A2SPG1
- ProtClustDB:   PRK05159
- BioCyc:   MLAB410358:MLAB_0039-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_A
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020780
- InterPro:   IPR004523
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00458

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 48294; Mature: 48294

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPIKDVTPEIGHARIAGWVHEERDLGGLTFLLVRDRTGILQVTIPKKKVTPEVLAAVK
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCHHCCHHHHHHHH
EATRESVIECEGVVKATEKAPGGRELVPDMLRVVSRAETPLPLDVSEKVPAELDTRLDNR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCC
YLDLRKPRINAIFQIRNACLRAISEYLWDNGFTQVQTPKIVAAATEGGTELFPLAYFDKE
EEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEEEECCCCCEEEEEHHCCHH
AFLNQSPQLYKQMLMSAGFDRVFEIGAIFRAEEHNTVRHLNEATSIDIEMSFANEEDAMH
HHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHCEEEEEEEECCCHHHHH
VLENVVASAYQYVADHCGDALATLGITDFKVPTVPFPRITYKEAIEISTAGGEPLVFGDD
HHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCCHHHHEEEECCCCCEEEECCC
LSTAAERIVGEKMGTMYFITEWPTSTRPFYTMPFEDRPEVCRAFDMMHPRMELTSGAQRC
HHHHHHHHHHHHCCCEEEEEECCCCCCCEEECCCCCCHHHHHHHHHCCCHHHHHCCHHHH
HIHSLLVEQIKAKGLNPDAFEFYLNPFRYGMPPHAGWGLGAERLVMTMLDLQNIREAVLF
HHHHHHHHHHHHCCCCCCHHHHEECHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
PRDRHRVSP
CCHHCCCCC
>Mature Secondary Structure
MRIPIKDVTPEIGHARIAGWVHEERDLGGLTFLLVRDRTGILQVTIPKKKVTPEVLAAVK
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCHHCCHHHHHHHH
EATRESVIECEGVVKATEKAPGGRELVPDMLRVVSRAETPLPLDVSEKVPAELDTRLDNR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCC
YLDLRKPRINAIFQIRNACLRAISEYLWDNGFTQVQTPKIVAAATEGGTELFPLAYFDKE
EEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEEEECCCCCEEEEEHHCCHH
AFLNQSPQLYKQMLMSAGFDRVFEIGAIFRAEEHNTVRHLNEATSIDIEMSFANEEDAMH
HHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHCEEEEEEEECCCHHHHH
VLENVVASAYQYVADHCGDALATLGITDFKVPTVPFPRITYKEAIEISTAGGEPLVFGDD
HHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCCHHHHEEEECCCCCEEEECCC
LSTAAERIVGEKMGTMYFITEWPTSTRPFYTMPFEDRPEVCRAFDMMHPRMELTSGAQRC
HHHHHHHHHHHHCCCEEEEEECCCCCCCEEECCCCCCHHHHHHHHHCCCHHHHHCCHHHH
HIHSLLVEQIKAKGLNPDAFEFYLNPFRYGMPPHAGWGLGAERLVMTMLDLQNIREAVLF
HHHHHHHHHHHHCCCCCCHHHHEECHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
PRDRHRVSP
CCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA