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The map label for this gene is glcF [H]

Identifier: 124266154

GI number: 124266154

Start: 1019249

End: 1020484

Strand: Direct

Name: glcF [H]

Synonym: Mpe_A0961

Alternate gene names: 124266154

Gene position: 1019249-1020484 (Clockwise)

Preceding gene: 124266153

Following gene: 124266156

Centisome position: 25.2

GC content: 71.44

Gene sequence:

>1236_bases
ATGCAAATCCAGTTGAGTCCCGAGTTCCACGGCGATCGCGAAGCCGCCGAAGCCGAAGCCATCCTGCGCAGCTGCGTGCA
CTGCGGGTTCTGCACGGCCACCTGTCCCACCTACCAGCTCGAGGGCAACGAACTCGACGGCCCGCGCGGGCGCATCTACC
TGATCAAGCAGGTGTTCGAGGGCGGCGAGGCCAGCCGCAGCACGCAGCGTCACCTGGACCGCTGCCTGACCTGCCGCAAC
TGCGAGACCACCTGCCCGAGCGGTGTGCACTACGGTCGGCTGGTCGACATCGGTCGCCAGGTCGTCGACGCCCGGGTGCA
GCGGCCGGTGGCCGAGCGCGCGGTCCGCTGGGTGCTGAAGGAAGGCCTGACGTCGCCGCTGGTCGGTCCGGCCGTGCGCC
TGGGGCAGCTGCTGCGGCCGGCGCTGCCCGGGGTGCTGAAGAAGAAGCTGCCGCGGCGCGCGGGCGCGCGAGCGTCGGAC
TGGCCTCGCACCGAGCACCCGCGCAAGGTGATGCTGCTGCAGGGCTGTGTGCAGCCGGCGATGCTGCCGAACATCAACGC
CGCGACGGCGCGCGTGCTCGATGCCGCCGGGATCCAGACCCTGGTCGCCGCGGAGGGCGGCTGTTGCGGGGCCTTGCGCA
CCCATCTGAGCGACGCCGAGGGCGGGCTGGCGGACATGCGTCGCAACATCGACGCATGGTGGCCTGCCGTCGAATCCGGC
GACGTGGAATGCATCCTCGTCAATGCCTCGGGCTGCGGCGCGGTGGTCAAGGAGTACGGCGAGGCCCTCGCGCACGAACC
GGCCTATGCCGAACGAGCCGCCCGCATCAGCGCCCTGGCCAGGGACCTGAGCGAACTGCTGCCCGACCTGCTGCCGGCCC
TGCGGGCCAGGATGCGGCCGCAGGGGGCCGAGCGCCGGGTGTCGGTGCACACGCCGTGCACGCTGCAACATGCGCAGCGA
CTGCGCGGCGGTGTCGAGGCCGCACTCGCGGCGCTCGGCTTCGACGTGACGGCTTCGGCCGGCGATGGACACCTGTGCTG
CGGCTCGGCCGGTGCCTACAGCGTGCTGCAGCCGGGCATCGCGACGGCGCTGCGCGATCGCAAGCTGTCGAGCCTGAACA
CGCACGAGCCGGACGTGATCGTCTCGGCCAACGTCGGGTGCATCCTGCACCTGCAGTCCGGGACGGCCGTGCCGGTGCAG
CATTGGGTGGAGATGGTGGATCAGGCCCTCGCCTGA

Upstream 100 bases:

>100_bases
TCGCCGGCGCTGCTGCAGCTGCACCATCGGCTCAAGCAGGCCTTCGATCCGGAGCGGCTGTTCAACCCGGGCCGGCTCCA
CGAAGGCCTCTGAGACCGTC

Downstream 100 bases:

>100_bases
GTCGTCACGTTCTTCCTGCGCATGCGATAGACCACCTGCGTCCGCGCCATGCCGATCAGCCGTGCCGCGGCGGCCATGTT
GCCGTTGCAGCGTTGCACCG

Product: glycolate oxidase iron-sulfur subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MQIQLSPEFHGDREAAEAEAILRSCVHCGFCTATCPTYQLEGNELDGPRGRIYLIKQVFEGGEASRSTQRHLDRCLTCRN
CETTCPSGVHYGRLVDIGRQVVDARVQRPVAERAVRWVLKEGLTSPLVGPAVRLGQLLRPALPGVLKKKLPRRAGARASD
WPRTEHPRKVMLLQGCVQPAMLPNINAATARVLDAAGIQTLVAAEGGCCGALRTHLSDAEGGLADMRRNIDAWWPAVESG
DVECILVNASGCGAVVKEYGEALAHEPAYAERAARISALARDLSELLPDLLPALRARMRPQGAERRVSVHTPCTLQHAQR
LRGGVEAALAALGFDVTASAGDGHLCCGSAGAYSVLQPGIATALRDRKLSSLNTHEPDVIVSANVGCILHLQSGTAVPVQ
HWVEMVDQALA

Sequences:

>Translated_411_residues
MQIQLSPEFHGDREAAEAEAILRSCVHCGFCTATCPTYQLEGNELDGPRGRIYLIKQVFEGGEASRSTQRHLDRCLTCRN
CETTCPSGVHYGRLVDIGRQVVDARVQRPVAERAVRWVLKEGLTSPLVGPAVRLGQLLRPALPGVLKKKLPRRAGARASD
WPRTEHPRKVMLLQGCVQPAMLPNINAATARVLDAAGIQTLVAAEGGCCGALRTHLSDAEGGLADMRRNIDAWWPAVESG
DVECILVNASGCGAVVKEYGEALAHEPAYAERAARISALARDLSELLPDLLPALRARMRPQGAERRVSVHTPCTLQHAQR
LRGGVEAALAALGFDVTASAGDGHLCCGSAGAYSVLQPGIATALRDRKLSSLNTHEPDVIVSANVGCILHLQSGTAVPVQ
HWVEMVDQALA
>Mature_411_residues
MQIQLSPEFHGDREAAEAEAILRSCVHCGFCTATCPTYQLEGNELDGPRGRIYLIKQVFEGGEASRSTQRHLDRCLTCRN
CETTCPSGVHYGRLVDIGRQVVDARVQRPVAERAVRWVLKEGLTSPLVGPAVRLGQLLRPALPGVLKKKLPRRAGARASD
WPRTEHPRKVMLLQGCVQPAMLPNINAATARVLDAAGIQTLVAAEGGCCGALRTHLSDAEGGLADMRRNIDAWWPAVESG
DVECILVNASGCGAVVKEYGEALAHEPAYAERAARISALARDLSELLPDLLPALRARMRPQGAERRVSVHTPCTLQHAQR
LRGGVEAALAALGFDVTASAGDGHLCCGSAGAYSVLQPGIATALRDRKLSSLNTHEPDVIVSANVGCILHLQSGTAVPVQ
HWVEMVDQALA

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=411, Percent_Identity=53.7712895377129, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1788576, Length=419, Percent_Identity=23.1503579952267, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786497, Length=247, Percent_Identity=25.9109311740891, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: 1.1.3.15

Molecular weight: Translated: 44026; Mature: 44026

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIQLSPEFHGDREAAEAEAILRSCVHCGFCTATCPTYQLEGNELDGPRGRIYLIKQVFE
CEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHCCCCCEEECCCCCCCCCCCEEEHHHHHC
GGEASRSTQRHLDRCLTCRNCETTCPSGVHYGRLVDIGRQVVDARVQRPVAERAVRWVLK
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
EGLTSPLVGPAVRLGQLLRPALPGVLKKKLPRRAGARASDWPRTEHPRKVMLLQGCVQPA
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCHHHHHHHHHCCHH
MLPNINAATARVLDAAGIQTLVAAEGGCCGALRTHLSDAEGGLADMRRNIDAWWPAVESG
HCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHCCHHHCCCCCCC
DVECILVNASGCGAVVKEYGEALAHEPAYAERAARISALARDLSELLPDLLPALRARMRP
CEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QGAERRVSVHTPCTLQHAQRLRGGVEAALAALGFDVTASAGDGHLCCGSAGAYSVLQPGI
CCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEECCCCCHHHHCCHH
ATALRDRKLSSLNTHEPDVIVSANVGCILHLQSGTAVPVQHWVEMVDQALA
HHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MQIQLSPEFHGDREAAEAEAILRSCVHCGFCTATCPTYQLEGNELDGPRGRIYLIKQVFE
CEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHCCCCCEEECCCCCCCCCCCEEEHHHHHC
GGEASRSTQRHLDRCLTCRNCETTCPSGVHYGRLVDIGRQVVDARVQRPVAERAVRWVLK
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
EGLTSPLVGPAVRLGQLLRPALPGVLKKKLPRRAGARASDWPRTEHPRKVMLLQGCVQPA
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCHHHHHHHHHCCHH
MLPNINAATARVLDAAGIQTLVAAEGGCCGALRTHLSDAEGGLADMRRNIDAWWPAVESG
HCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHCCHHHCCCCCCC
DVECILVNASGCGAVVKEYGEALAHEPAYAERAARISALARDLSELLPDLLPALRARMRP
CEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QGAERRVSVHTPCTLQHAQRLRGGVEAALAALGFDVTASAGDGHLCCGSAGAYSVLQPGI
CCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEECCCCCHHHHCCHH
ATALRDRKLSSLNTHEPDVIVSANVGCILHLQSGTAVPVQHWVEMVDQALA
HHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]