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The map label for this gene is gloB [H]

Identifier: 124266127

GI number: 124266127

Start: 988437

End: 989216

Strand: Direct

Name: gloB [H]

Synonym: Mpe_A0934

Alternate gene names: 124266127

Gene position: 988437-989216 (Clockwise)

Preceding gene: 124266126

Following gene: 124266128

Centisome position: 24.44

GC content: 69.74

Gene sequence:

>780_bases
GTGCCCGCCCTCGTGTCGATCAAGGCCTTTGCCGACAACTACATCTGGTTGTTTCGCGTCGGGAACCGCGCGTGGGTCGT
CGACCCGGGCGAGTCGGCCGGCGTGCTCGGCTATCTGGACAAGCACCACCTGATCCTCGAAGGGATCCTGCTGACCCACC
ACCACGACGACCACACGGGTGGCGTCGAACACTTGCGATCGGTGCGCAACGCCCGGGTCTACGGGCCTGCGCGCGAGCGC
CTGCCCGAGCCCGTCATCCGGCTGCTGCACGGCGACGAGGTGGACGCCCTGGGCGTGCGCTTCCGCGTGATCGACGTGCC
GGGGCACACCGCCGGGCACATCGCCTATTACGCCGAAGACTTCGACGGTCGTCCGCTGCTGTTCTGCGGCGACACGCTGT
TCTCCGGCGGCTGCGGGCGCCTGTTCGAGGGCACGCCGGCGCAGATGTTCGCGTCGCTCGACCGGCTCGCCGGCCTGCCC
GGAGACACTCTCGTGTGCTGTGCCCACGAGTACACGGTGGGCAACCTGCGGTTCGCGAACGCTGTCGAGCCCGGCAATCG
CGACGTGGTGGAGCACCTCGATCGATGCCTGGCGCTGCGGGCCCGCGACGAGCCCACGCTGCCCAGTTCCATCAAGCAGG
AGCGCCTGATCAACCCCTTCCTGCGCTGCCAGGACCCGGCGGTGGCGAACGCGGCGCGGCGCCGGGCCGCGGTCGGCCAT
GCGATGGACGACGTGGCGGTGTTCGCTGCGTTGAGGGCCTGGAAGGACCGCTTCGCATGA

Upstream 100 bases:

>100_bases
AGGCCCGGGCCGGAGGTCCGGGAACGATGCGTCGCGCGTCCGACCTCGATCAGGTCCCGCGCGGCTCGGTGAGTTTGCTC
AGCGCTGCCAGGAGAGGAAC

Downstream 100 bases:

>100_bases
GGCCTCGACCATGGCGATGAGGGTGTCCTTCTTCCGCGCGCGCCGGGACGCACCGCAGGAACCTGACGAGAAGGGCGCCG
AGGTGGCACGGTTGCACTGG

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MPALVSIKAFADNYIWLFRVGNRAWVVDPGESAGVLGYLDKHHLILEGILLTHHHDDHTGGVEHLRSVRNARVYGPARER
LPEPVIRLLHGDEVDALGVRFRVIDVPGHTAGHIAYYAEDFDGRPLLFCGDTLFSGGCGRLFEGTPAQMFASLDRLAGLP
GDTLVCCAHEYTVGNLRFANAVEPGNRDVVEHLDRCLALRARDEPTLPSSIKQERLINPFLRCQDPAVANAARRRAAVGH
AMDDVAVFAALRAWKDRFA

Sequences:

>Translated_259_residues
MPALVSIKAFADNYIWLFRVGNRAWVVDPGESAGVLGYLDKHHLILEGILLTHHHDDHTGGVEHLRSVRNARVYGPARER
LPEPVIRLLHGDEVDALGVRFRVIDVPGHTAGHIAYYAEDFDGRPLLFCGDTLFSGGCGRLFEGTPAQMFASLDRLAGLP
GDTLVCCAHEYTVGNLRFANAVEPGNRDVVEHLDRCLALRARDEPTLPSSIKQERLINPFLRCQDPAVANAARRRAAVGH
AMDDVAVFAALRAWKDRFA
>Mature_258_residues
PALVSIKAFADNYIWLFRVGNRAWVVDPGESAGVLGYLDKHHLILEGILLTHHHDDHTGGVEHLRSVRNARVYGPARERL
PEPVIRLLHGDEVDALGVRFRVIDVPGHTAGHIAYYAEDFDGRPLLFCGDTLFSGGCGRLFEGTPAQMFASLDRLAGLPG
DTLVCCAHEYTVGNLRFANAVEPGNRDVVEHLDRCLALRARDEPTLPSSIKQERLINPFLRCQDPAVANAARRRAAVGHA
MDDVAVFAALRAWKDRFA

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=258, Percent_Identity=36.046511627907, Blast_Score=152, Evalue=2e-37,
Organism=Homo sapiens, GI94538322, Length=258, Percent_Identity=36.046511627907, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI14150041, Length=226, Percent_Identity=38.0530973451327, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI116642887, Length=233, Percent_Identity=38.1974248927039, Blast_Score=141, Evalue=6e-34,
Organism=Homo sapiens, GI21703352, Length=233, Percent_Identity=38.1974248927039, Blast_Score=141, Evalue=7e-34,
Organism=Homo sapiens, GI46361987, Length=192, Percent_Identity=37.5, Blast_Score=117, Evalue=9e-27,
Organism=Escherichia coli, GI1786406, Length=257, Percent_Identity=44.3579766536965, Blast_Score=197, Evalue=6e-52,
Organism=Escherichia coli, GI1787158, Length=205, Percent_Identity=30.7317073170732, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17536925, Length=267, Percent_Identity=34.0823970037453, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320478, Length=272, Percent_Identity=31.6176470588235, Blast_Score=103, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324614, Length=252, Percent_Identity=30.1587301587302, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21356335, Length=257, Percent_Identity=38.1322957198444, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24667703, Length=259, Percent_Identity=37.8378378378378, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24667711, Length=259, Percent_Identity=37.8378378378378, Blast_Score=166, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28587; Mature: 28456

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPALVSIKAFADNYIWLFRVGNRAWVVDPGESAGVLGYLDKHHLILEGILLTHHHDDHTG
CCCEEEEEECCCCEEEEEEECCEEEEECCCCCCCEEEECCCCHHHHHEEEEEECCCCCCC
GVEHLRSVRNARVYGPARERLPEPVIRLLHGDEVDALGVRFRVIDVPGHTAGHIAYYAED
HHHHHHHHHHCEEECCHHHHCHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCEEEEEEEC
FDGRPLLFCGDTLFSGGCGRLFEGTPAQMFASLDRLAGLPGDTLVCCAHEYTVGNLRFAN
CCCCEEEEECCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEECCEEEEC
AVEPGNRDVVEHLDRCLALRARDEPTLPSSIKQERLINPFLRCQDPAVANAARRRAAVGH
CCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHH
AMDDVAVFAALRAWKDRFA
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PALVSIKAFADNYIWLFRVGNRAWVVDPGESAGVLGYLDKHHLILEGILLTHHHDDHTG
CCEEEEEECCCCEEEEEEECCEEEEECCCCCCCEEEECCCCHHHHHEEEEEECCCCCCC
GVEHLRSVRNARVYGPARERLPEPVIRLLHGDEVDALGVRFRVIDVPGHTAGHIAYYAED
HHHHHHHHHHCEEECCHHHHCHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCEEEEEEEC
FDGRPLLFCGDTLFSGGCGRLFEGTPAQMFASLDRLAGLPGDTLVCCAHEYTVGNLRFAN
CCCCEEEEECCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEECCEEEEC
AVEPGNRDVVEHLDRCLALRARDEPTLPSSIKQERLINPFLRCQDPAVANAARRRAAVGH
CCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHH
AMDDVAVFAALRAWKDRFA
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA