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The map label for this gene is btuF [H]

Identifier: 124266097

GI number: 124266097

Start: 958033

End: 958842

Strand: Direct

Name: btuF [H]

Synonym: Mpe_A0904

Alternate gene names: 124266097

Gene position: 958033-958842 (Clockwise)

Preceding gene: 124266096

Following gene: 124266098

Centisome position: 23.69

GC content: 72.72

Gene sequence:

>810_bases
ATGACGTCCATGCCTGCCCGCTTTCCCGAACGCATCGTCTGCCTCACCGAGGAGCCCACCGAGGTGCTCTACGCGCTGGG
CGAGGCGCACCGCATCGTCGGCATCTCCGGCTTCACCGTGCGTCCGCCACGCGCGCGGCAGGAGAAGCCCAAGGTGTCGG
CCTTCACCAGCGCGAAGCTCGAGCCGATCCTGGCGCTGCAGCCCGACCTGGCGATCGGCTTCTCCGACATCCAGGCCGAC
ATCGCCCGCGAGCTGATCCAGCGCGGCGTGGAGGTGTGGATCAGCAACCACCGCTCGGTCGACGGCATCCTGGGTTATGT
GCGCCGGCTCGGCGCGATGGTCGGCGCGGCGCACCGGGCCGAGGCCTATGCTGACGCGCTGGCCGCGCGCGTGGAGCGCG
TGCGGCAGGCCGCCGCGCAGCTGCCGCGCCGCCCGCGCGTCTACTTCGAGGAGTGGGACGAGCCGCAGATCAGCTGCATC
CGCTGGGTCAGCGAGCTGGTGGGCATCGCGGGCGGCGACGACATCTTTCCCGAGCGCGCCGTGGCGAGCCTGGCGCGCGA
CCGCATCCTCGCGACGCCCGACGAGGTGATCCCGCGCGCGCCCGACATCGTCATCGGCTCGTGGTGCGGCAAGAAGTTCC
GCCCCGAGAAGGTGGCCGCCCGTGCCGGCTGGGACCAAGTGCCCGCGGTGCGCGACCGCGAGCTGCACGAGATCAAGTCG
CCGCTGATCCTGCAGCCGGGCCCGGCCGCGCTGACCGACGGGCTGGACGCGCTGCACGCGATCGTGGCGCGCTGGGCGGC
CCGGCACTGA

Upstream 100 bases:

>100_bases
TGCTCGCTGGGGCAGATCACCACCGCGCTGTTCGAGGTGGGTGGGCAGTACCGGCGCAGCATGTGAGCGGCGCGCCGCCC
GGCGCGGGCCGGCCGCGACA

Downstream 100 bases:

>100_bases
GCCCGCCGGCGCGCGCGCCGCGTCGCCATGGATCAGGCGTTCAAGCCTACCCACCCGGGTGGAAAACTACCCTCGCGGGT
AGTGGCCTGCGGCGACGCGG

Product: putative substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MTSMPARFPERIVCLTEEPTEVLYALGEAHRIVGISGFTVRPPRARQEKPKVSAFTSAKLEPILALQPDLAIGFSDIQAD
IARELIQRGVEVWISNHRSVDGILGYVRRLGAMVGAAHRAEAYADALAARVERVRQAAAQLPRRPRVYFEEWDEPQISCI
RWVSELVGIAGGDDIFPERAVASLARDRILATPDEVIPRAPDIVIGSWCGKKFRPEKVAARAGWDQVPAVRDRELHEIKS
PLILQPGPAALTDGLDALHAIVARWAARH

Sequences:

>Translated_269_residues
MTSMPARFPERIVCLTEEPTEVLYALGEAHRIVGISGFTVRPPRARQEKPKVSAFTSAKLEPILALQPDLAIGFSDIQAD
IARELIQRGVEVWISNHRSVDGILGYVRRLGAMVGAAHRAEAYADALAARVERVRQAAAQLPRRPRVYFEEWDEPQISCI
RWVSELVGIAGGDDIFPERAVASLARDRILATPDEVIPRAPDIVIGSWCGKKFRPEKVAARAGWDQVPAVRDRELHEIKS
PLILQPGPAALTDGLDALHAIVARWAARH
>Mature_268_residues
TSMPARFPERIVCLTEEPTEVLYALGEAHRIVGISGFTVRPPRARQEKPKVSAFTSAKLEPILALQPDLAIGFSDIQADI
ARELIQRGVEVWISNHRSVDGILGYVRRLGAMVGAAHRAEAYADALAARVERVRQAAAQLPRRPRVYFEEWDEPQISCIR
WVSELVGIAGGDDIFPERAVASLARDRILATPDEVIPRAPDIVIGSWCGKKFRPEKVAARAGWDQVPAVRDRELHEIKSP
LILQPGPAALTDGLDALHAIVARWAARH

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=241, Percent_Identity=24.4813278008299, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 29718; Mature: 29586

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSMPARFPERIVCLTEEPTEVLYALGEAHRIVGISGFTVRPPRARQEKPKVSAFTSAKL
CCCCCCCCCCCEEEECCCHHHHHHHHHHHHEEEECCCCEECCCCCCCCCCCHHHHHHCCC
EPILALQPDLAIGFSDIQADIARELIQRGVEVWISNHRSVDGILGYVRRLGAMVGAAHRA
CCCEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
EAYADALAARVERVRQAAAQLPRRPRVYFEEWDEPQISCIRWVSELVGIAGGDDIFPERA
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHH
VASLARDRILATPDEVIPRAPDIVIGSWCGKKFRPEKVAARAGWDQVPAVRDRELHEIKS
HHHHHHHHHCCCCHHHCCCCCCEEEECCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHCC
PLILQPGPAALTDGLDALHAIVARWAARH
CEEECCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSMPARFPERIVCLTEEPTEVLYALGEAHRIVGISGFTVRPPRARQEKPKVSAFTSAKL
CCCCCCCCCCEEEECCCHHHHHHHHHHHHEEEECCCCEECCCCCCCCCCCHHHHHHCCC
EPILALQPDLAIGFSDIQADIARELIQRGVEVWISNHRSVDGILGYVRRLGAMVGAAHRA
CCCEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
EAYADALAARVERVRQAAAQLPRRPRVYFEEWDEPQISCIRWVSELVGIAGGDDIFPERA
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHH
VASLARDRILATPDEVIPRAPDIVIGSWCGKKFRPEKVAARAGWDQVPAVRDRELHEIKS
HHHHHHHHHCCCCHHHCCCCCCEEEECCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHCC
PLILQPGPAALTDGLDALHAIVARWAARH
CEEECCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]