Click here to switch to the map view.

The map label for this gene is bepF [H]

Identifier: 124266062

GI number: 124266062

Start: 907688

End: 908917

Strand: Reverse

Name: bepF [H]

Synonym: Mpe_A0869

Alternate gene names: 124266062

Gene position: 908917-907688 (Counterclockwise)

Preceding gene: 124266063

Following gene: 124266061

Centisome position: 22.47

GC content: 73.82

Gene sequence:

>1230_bases
ATGAACCAGCCCTCTTCCTCTCCCAGCCCGCGCCGCCTGTGGGCCGCCGGCACCGCCCTCACCGCGCTGATCGCGGTGTC
GGCCGCCTTCTTCGGACTCAACAGCCCCAACGCCGAGGCCAACCCGGCACCGACCGCCCCCGCGGCGACGCCGGTCTCGG
TGGCCACCGTCGTGCAGAGCCAGGTCGCGGCGTGGGACGAGTTCTCCGGCCGCCTCGAAGCGGTGGAACGGGTCGACGTG
CGCTCGCGCGTGGCCGGCGCGGTGCAGTCGGTGCACTTCCGCGAGGGCGCGCTGGTCAAGCAGGGCGAGCTGCTGATCAC
CATCGACCCGGCGCCCTATGCGGCCGACGTGGAGCGCGCCGAGGCGCAGGTGGTCGCCGCCCAGGCGCGCGTGGCGTACA
CGCGCAGCGAGCGTGACCGCGCCCAGCGCCTGTGGGACGAGCGCGCCATCGCGCAGCGCGAGTACGACGAGCGCGTGAGC
GGCCAGCTCGAGGCCGATGCCAGCCTGCGCGCCGCGCAGGCCGCACTGCAGTCGGCGCGCCTGAACCTCGGCTACACGCA
GGTGCGTGCGCCGGTGGCCGGCCGCGTGGGCCGGCTCGAGATCACCGTCGGCAACCTGGTGGCGGCCGGCCCCGGCGCGC
CGGTGCTGACCACGCTGGTGTCGGTGAGCCCGATCTACGCGAGCTTCGACGCCGACGAGCAGGTGGTGGCGCGCGCGCTG
AAGGATCTGCCGAGCGGCGCAAGCGCGCGCAGCCAGCTCGAGCGCATCCCGGTGCAGATGGGCACCGCCGCGAACAGCGA
CACGCCGCGGCTCGGCAAGCTGCAGCTGGTGGACAACCAGGTCGACGCGAAGAGCGGCACGGTGCGCGTGCGCGCCGTGT
TCGACAACGCCGACGGCAGTCTGATGCCCGGCCAGTTCGCGCGGCTGCGCATGGGCCAGGCCCGCAACGGCAGCGCGCTG
TTGGTCAACGAGCGCGCGGTCGGCACCGACCAGAGCAAGAAGTTCGTGATGGTGATCGGCGCCGACAACCGCGCCGAGTA
CCGCGAGGTGCGGCTCGGTGCCCACGTCGACGGACTGCGCGTCGTGACCGACGGCCTGAAGGCCGACGAGCGCATCGTCG
TCAACGGCCTGCAGCGCATCCGTCCGGGCGCGCTGGTCGCGCCCAAGCCGGTGGCGATGGACGCCAAGCCCGAGCTGCAG
GCGCGCAGCGACGCGGCCCCGCAATCCTGA

Upstream 100 bases:

>100_bases
GCGGGCAGTTCCAGGCCTTTCTGCAGCGCGCTCATGCGCCGGCCGCCGTTCCAGTTTCCGCGTCTTCCTCCATCCAGAAC
CGCCGCCCCGGGAGCCTCTC

Downstream 100 bases:

>100_bases
TGACCTGAACCGACCGGGGTCCCGACCCCCGGCGTCTCCGCCGTATCCCCGAGGGAGGGAGCCATGAACCTGTCCAAGTT
TTTCATCGATCGGCCGATCT

Product: RND multidrug efflux membrane fusion protein MexE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MNQPSSSPSPRRLWAAGTALTALIAVSAAFFGLNSPNAEANPAPTAPAATPVSVATVVQSQVAAWDEFSGRLEAVERVDV
RSRVAGAVQSVHFREGALVKQGELLITIDPAPYAADVERAEAQVVAAQARVAYTRSERDRAQRLWDERAIAQREYDERVS
GQLEADASLRAAQAALQSARLNLGYTQVRAPVAGRVGRLEITVGNLVAAGPGAPVLTTLVSVSPIYASFDADEQVVARAL
KDLPSGASARSQLERIPVQMGTAANSDTPRLGKLQLVDNQVDAKSGTVRVRAVFDNADGSLMPGQFARLRMGQARNGSAL
LVNERAVGTDQSKKFVMVIGADNRAEYREVRLGAHVDGLRVVTDGLKADERIVVNGLQRIRPGALVAPKPVAMDAKPELQ
ARSDAAPQS

Sequences:

>Translated_409_residues
MNQPSSSPSPRRLWAAGTALTALIAVSAAFFGLNSPNAEANPAPTAPAATPVSVATVVQSQVAAWDEFSGRLEAVERVDV
RSRVAGAVQSVHFREGALVKQGELLITIDPAPYAADVERAEAQVVAAQARVAYTRSERDRAQRLWDERAIAQREYDERVS
GQLEADASLRAAQAALQSARLNLGYTQVRAPVAGRVGRLEITVGNLVAAGPGAPVLTTLVSVSPIYASFDADEQVVARAL
KDLPSGASARSQLERIPVQMGTAANSDTPRLGKLQLVDNQVDAKSGTVRVRAVFDNADGSLMPGQFARLRMGQARNGSAL
LVNERAVGTDQSKKFVMVIGADNRAEYREVRLGAHVDGLRVVTDGLKADERIVVNGLQRIRPGALVAPKPVAMDAKPELQ
ARSDAAPQS
>Mature_409_residues
MNQPSSSPSPRRLWAAGTALTALIAVSAAFFGLNSPNAEANPAPTAPAATPVSVATVVQSQVAAWDEFSGRLEAVERVDV
RSRVAGAVQSVHFREGALVKQGELLITIDPAPYAADVERAEAQVVAAQARVAYTRSERDRAQRLWDERAIAQREYDERVS
GQLEADASLRAAQAALQSARLNLGYTQVRAPVAGRVGRLEITVGNLVAAGPGAPVLTTLVSVSPIYASFDADEQVVARAL
KDLPSGASARSQLERIPVQMGTAANSDTPRLGKLQLVDNQVDAKSGTVRVRAVFDNADGSLMPGQFARLRMGQARNGSAL
LVNERAVGTDQSKKFVMVIGADNRAEYREVRLGAHVDGLRVVTDGLKADERIVVNGLQRIRPGALVAPKPVAMDAKPELQ
ARSDAAPQS

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=391, Percent_Identity=32.4808184143223, Blast_Score=174, Evalue=7e-45,
Organism=Escherichia coli, GI1789929, Length=356, Percent_Identity=31.4606741573034, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI87082036, Length=353, Percent_Identity=30.028328611898, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1789665, Length=375, Percent_Identity=31.4666666666667, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI87081796, Length=179, Percent_Identity=30.1675977653631, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI87081951, Length=157, Percent_Identity=33.1210191082803, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43358; Mature: 43358

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQPSSSPSPRRLWAAGTALTALIAVSAAFFGLNSPNAEANPAPTAPAATPVSVATVVQS
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
QVAAWDEFSGRLEAVERVDVRSRVAGAVQSVHFREGALVKQGELLITIDPAPYAADVERA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEECCCCCCHHHHHH
EAQVVAAQARVAYTRSERDRAQRLWDERAIAQREYDERVSGQLEADASLRAAQAALQSAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LNLGYTQVRAPVAGRVGRLEITVGNLVAAGPGAPVLTTLVSVSPIYASFDADEQVVARAL
CCCCHHHHCCCCCCCCEEEEEEECCEEECCCCCHHHHHHHHHCCCEECCCCCHHHHHHHH
KDLPSGASARSQLERIPVQMGTAANSDTPRLGKLQLVDNQVDAKSGTVRVRAVFDNADGS
HHCCCCCHHHHHHHHCCHHCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCC
LMPGQFARLRMGQARNGSALLVNERAVGTDQSKKFVMVIGADNRAEYREVRLGAHVDGLR
CCCCHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCE
VVTDGLKADERIVVNGLQRIRPGALVAPKPVAMDAKPELQARSDAAPQS
EEECCCCCCCHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNQPSSSPSPRRLWAAGTALTALIAVSAAFFGLNSPNAEANPAPTAPAATPVSVATVVQS
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
QVAAWDEFSGRLEAVERVDVRSRVAGAVQSVHFREGALVKQGELLITIDPAPYAADVERA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEECCCCCCHHHHHH
EAQVVAAQARVAYTRSERDRAQRLWDERAIAQREYDERVSGQLEADASLRAAQAALQSAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LNLGYTQVRAPVAGRVGRLEITVGNLVAAGPGAPVLTTLVSVSPIYASFDADEQVVARAL
CCCCHHHHCCCCCCCCEEEEEEECCEEECCCCCHHHHHHHHHCCCEECCCCCHHHHHHHH
KDLPSGASARSQLERIPVQMGTAANSDTPRLGKLQLVDNQVDAKSGTVRVRAVFDNADGS
HHCCCCCHHHHHHHHCCHHCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCC
LMPGQFARLRMGQARNGSALLVNERAVGTDQSKKFVMVIGADNRAEYREVRLGAHVDGLR
CCCCHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCE
VVTDGLKADERIVVNGLQRIRPGALVAPKPVAMDAKPELQARSDAAPQS
EEECCCCCCCHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]