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The map label for this gene is est [H]

Identifier: 124266063

GI number: 124266063

Start: 908914

End: 909828

Strand: Reverse

Name: est [H]

Synonym: Mpe_A0870

Alternate gene names: 124266063

Gene position: 909828-908914 (Counterclockwise)

Preceding gene: 124266066

Following gene: 124266062

Centisome position: 22.5

GC content: 75.96

Gene sequence:

>915_bases
ATGTCCACCCCCCCTTCCCTGCCCGCCCCGCTCGCTCCGCCCCTTGGCGGCGCGTTGGCCTGGGCAGACCAGTGGATTCC
AGTCGGCGCCGGCGAACCGCTGAAGGCGCGCGTCTACGGCAGCCGCCCGCGCGGCCGCTCGGCGCCGCTGGTGCTGCACT
TCCACGGCGGGGCCTTCGTGGCTGGCGGGCTGGACAGCGGCGCCACGGTCGCCGGCCTGCTGGCCGAGGCCGGCGCGGTG
GTCGTGTCGCTCGACTACCCGCTGGCGCCCGCCCACCCCTTCCCCGCCGCCATCGAGGCCGGCCACGCCGCGCTCACCTG
GATGCACCGCCACCGCAGCCGGCTGGCCGGCCAGAGCGCGACGGTCTGGGTCGCCGGCGAAGAGGCGGGTGGCAACCTGG
CCGCGGCCGTGGCACTGATGGCGCGCGACCGGCGCAGCCCGCCGCTGGCCGGCCAGATCCTGATGTCGCCGATGCTCGAT
CCCTGCGTGGCCACCGCCTCGCTGCGCGATGCCGATGCCGGCCCCGTCGGTTGCCTGTGGGCCGACGGCTGGCACCAGTA
CCTGCCCGGCGCCGCCGACGCCGACCACCCGTATGCGGCGCCCGCTTCGGCGATGCGGCTGACCGGCGTGCCGCGCACGC
TGCTGATCACCGCGCAGGACGACCCGTTGCGCGACGAGGCGCGGGCCTATGCACGGCGGCTGCGCGACGCCGGCGTCGAG
GTCGATGAGGCGTTTCTCGACCAACCCACCGGCTGGCCCTGCGCCTGCAAGCAGCCCGCCGGCGCGCAGGCCGCCTGGGC
GCCCGTGGTCCGCGGGCAGTTCCAGGCCTTTCTGCAGCGCGCTCATGCGCCGGCCGCCGTTCCAGTTTCCGCGTCTTCCT
CCATCCAGAACCGCCGCCCCGGGAGCCTCTCATGA

Upstream 100 bases:

>100_bases
CAAAGCATGAGCAATGCGGCTATTTATTGTTTCAACGACCGCCACTATCTTCTGCTGCATCGCAACACGCTTCATGCCGC
TCCGGCGGCCCGAGGTCCAC

Downstream 100 bases:

>100_bases
ACCAGCCCTCTTCCTCTCCCAGCCCGCGCCGCCTGTGGGCCGCCGGCACCGCCCTCACCGCGCTGATCGCGGTGTCGGCC
GCCTTCTTCGGACTCAACAG

Product: putative esterase protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSTPPSLPAPLAPPLGGALAWADQWIPVGAGEPLKARVYGSRPRGRSAPLVLHFHGGAFVAGGLDSGATVAGLLAEAGAV
VVSLDYPLAPAHPFPAAIEAGHAALTWMHRHRSRLAGQSATVWVAGEEAGGNLAAAVALMARDRRSPPLAGQILMSPMLD
PCVATASLRDADAGPVGCLWADGWHQYLPGAADADHPYAAPASAMRLTGVPRTLLITAQDDPLRDEARAYARRLRDAGVE
VDEAFLDQPTGWPCACKQPAGAQAAWAPVVRGQFQAFLQRAHAPAAVPVSASSSIQNRRPGSLS

Sequences:

>Translated_304_residues
MSTPPSLPAPLAPPLGGALAWADQWIPVGAGEPLKARVYGSRPRGRSAPLVLHFHGGAFVAGGLDSGATVAGLLAEAGAV
VVSLDYPLAPAHPFPAAIEAGHAALTWMHRHRSRLAGQSATVWVAGEEAGGNLAAAVALMARDRRSPPLAGQILMSPMLD
PCVATASLRDADAGPVGCLWADGWHQYLPGAADADHPYAAPASAMRLTGVPRTLLITAQDDPLRDEARAYARRLRDAGVE
VDEAFLDQPTGWPCACKQPAGAQAAWAPVVRGQFQAFLQRAHAPAAVPVSASSSIQNRRPGSLS
>Mature_303_residues
STPPSLPAPLAPPLGGALAWADQWIPVGAGEPLKARVYGSRPRGRSAPLVLHFHGGAFVAGGLDSGATVAGLLAEAGAVV
VSLDYPLAPAHPFPAAIEAGHAALTWMHRHRSRLAGQSATVWVAGEEAGGNLAAAVALMARDRRSPPLAGQILMSPMLDP
CVATASLRDADAGPVGCLWADGWHQYLPGAADADHPYAAPASAMRLTGVPRTLLITAQDDPLRDEARAYARRLRDAGVEV
DEAFLDQPTGWPCACKQPAGAQAAWAPVVRGQFQAFLQRAHAPAAVPVSASSSIQNRRPGSLS

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21328446, Length=100, Percent_Identity=43, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1786682, Length=222, Percent_Identity=29.2792792792793, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 31466; Mature: 31335

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPPSLPAPLAPPLGGALAWADQWIPVGAGEPLKARVYGSRPRGRSAPLVLHFHGGAFV
CCCCCCCCCCCCCCCCCHHHHHCCEEECCCCCCEEEEEECCCCCCCCCCEEEEECCCEEE
AGGLDSGATVAGLLAEAGAVVVSLDYPLAPAHPFPAAIEAGHAALTWMHRHRSRLAGQSA
ECCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCE
TVWVAGEEAGGNLAAAVALMARDRRSPPLAGQILMSPMLDPCVATASLRDADAGPVGCLW
EEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
ADGWHQYLPGAADADHPYAAPASAMRLTGVPRTLLITAQDDPLRDEARAYARRLRDAGVE
CCCHHHHCCCCCCCCCCCCCCCHHEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCC
VDEAFLDQPTGWPCACKQPAGAQAAWAPVVRGQFQAFLQRAHAPAAVPVSASSSIQNRRP
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCC
GSLS
CCCC
>Mature Secondary Structure 
STPPSLPAPLAPPLGGALAWADQWIPVGAGEPLKARVYGSRPRGRSAPLVLHFHGGAFV
CCCCCCCCCCCCCCCCHHHHHCCEEECCCCCCEEEEEECCCCCCCCCCEEEEECCCEEE
AGGLDSGATVAGLLAEAGAVVVSLDYPLAPAHPFPAAIEAGHAALTWMHRHRSRLAGQSA
ECCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCE
TVWVAGEEAGGNLAAAVALMARDRRSPPLAGQILMSPMLDPCVATASLRDADAGPVGCLW
EEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
ADGWHQYLPGAADADHPYAAPASAMRLTGVPRTLLITAQDDPLRDEARAYARRLRDAGVE
CCCHHHHCCCCCCCCCCCCCCCHHEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCC
VDEAFLDQPTGWPCACKQPAGAQAAWAPVVRGQFQAFLQRAHAPAAVPVSASSSIQNRRP
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCC
GSLS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]