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The map label for this gene is hisF

Identifier: 124266029

GI number: 124266029

Start: 875579

End: 876340

Strand: Direct

Name: hisF

Synonym: Mpe_A0836

Alternate gene names: 124266029

Gene position: 875579-876340 (Clockwise)

Preceding gene: 124266028

Following gene: 124266030

Centisome position: 21.65

GC content: 69.69

Gene sequence:

>762_bases
ATGCTCGCCAAGCGCATCATCCCCTGCCTCGACGTCACCGGCGGCCGCGTCGTCAAGGGCGTCAACTTCGTCGAGTTGCG
TGATGCCGGCGACCCGGTGGAGATCGCCGCGCGCTACAACGAGCAGGGCGCCGACGAACTGACCTTCCTCGACATCACCG
CCACCAGCGACGGGCGCGACCTCATCCTGCCCATCATCGAGGCGGTGGCCTCGCAGGTCTTCATCCCGCTCACCGTCGGC
GGCGGAGTGCGCAGCGTCGAGGACGTGCGCCGCCTGCTCAATGCCGGGGCCGACAAGGTGAGTTTCAACTCGGCGGCGGT
CGCGAACCCGCAGCTGATCCGTGACGCCTCCGACAAGTACGGCGCGCAGTGCATCGTGGTCGCCATCGATGCCAAGGCCC
GGGTGGGCGGCGAGGGCTGGGAGGTCTACACGCACGGCGGACGCAGGAACACCGGCCTCGATGCAGTGGCCTGGGCGCAG
CAGATGGCCGAGTTCGGCGCCGGCGAGATCCTGCTGACCAGCATGGACCGCGACGGCACGCAGATCGGCTTCAACCTGCC
GCTGACGCGCGCGGTCAGCGACGCGGTGCCGGTGCCGGTGATCGCCTCGGGCGGCGTCGGCGCGCTGGAGCACCTCGCCG
AGGGCATCCAGGTCGGTGGCGCCGATGCGGTGCTGGCCGCCAGCATCTTCCACTACGGCACGTTCACCGTCGGCCAGGCC
AAGGCCTTGATGGCCGAGCACGGCATCCCCGTCAGGATGTAA

Upstream 100 bases:

>100_bases
GTGATCTGCGGTCGGGCGATCTACACCGGCGACCTCGACTTCGCCGCGGCGCAGGCGCGGGCCGACGCGCTGAACGGCGA
CGCCTGAGCGGCGCCCCCGC

Downstream 100 bases:

>100_bases
TCCACCCCCGTTGCGTGTGTGGCCCATCCCCGTTGCGGCTGCGCCGCTCCCCCTCGAGGGGGCGACGCTAGTGGACCGGC
GGAGCCGGATCCACGGCGTC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MLAKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNEQGADELTFLDITATSDGRDLILPIIEAVASQVFIPLTVG
GGVRSVEDVRRLLNAGADKVSFNSAAVANPQLIRDASDKYGAQCIVVAIDAKARVGGEGWEVYTHGGRRNTGLDAVAWAQ
QMAEFGAGEILLTSMDRDGTQIGFNLPLTRAVSDAVPVPVIASGGVGALEHLAEGIQVGGADAVLAASIFHYGTFTVGQA
KALMAEHGIPVRM

Sequences:

>Translated_253_residues
MLAKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNEQGADELTFLDITATSDGRDLILPIIEAVASQVFIPLTVG
GGVRSVEDVRRLLNAGADKVSFNSAAVANPQLIRDASDKYGAQCIVVAIDAKARVGGEGWEVYTHGGRRNTGLDAVAWAQ
QMAEFGAGEILLTSMDRDGTQIGFNLPLTRAVSDAVPVPVIASGGVGALEHLAEGIQVGGADAVLAASIFHYGTFTVGQA
KALMAEHGIPVRM
>Mature_253_residues
MLAKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNEQGADELTFLDITATSDGRDLILPIIEAVASQVFIPLTVG
GGVRSVEDVRRLLNAGADKVSFNSAAVANPQLIRDASDKYGAQCIVVAIDAKARVGGEGWEVYTHGGRRNTGLDAVAWAQ
QMAEFGAGEILLTSMDRDGTQIGFNLPLTRAVSDAVPVPVIASGGVGALEHLAEGIQVGGADAVLAASIFHYGTFTVGQA
KALMAEHGIPVRM

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=45.136186770428, Blast_Score=217, Evalue=7e-58,
Organism=Escherichia coli, GI87082028, Length=241, Percent_Identity=32.7800829875519, Blast_Score=106, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=35.8730158730159, Blast_Score=159, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_METPP (A2SE09)

Other databases:

- EMBL:   CP000555
- RefSeq:   YP_001020033.1
- ProteinModelPortal:   A2SE09
- SMR:   A2SE09
- STRING:   A2SE09
- GeneID:   4786971
- GenomeReviews:   CP000555_GR
- KEGG:   mpt:Mpe_A0836
- NMPDR:   fig|279263.3.peg.2039
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   A2SE09
- BioCyc:   MPET420662:MPE_A0836-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.- [C]

Molecular weight: Translated: 26450; Mature: 26450

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNEQGADELTFLDITATSDGRD
CCHHHCCCCCCCCCCEEEECCCEEEEECCCCCEEEEEECCCCCCCCEEEEEEEECCCCCC
LILPIIEAVASQVFIPLTVGGGVRSVEDVRRLLNAGADKVSFNSAAVANPQLIRDASDKY
HHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHCCCCCEECCCCCCCCCHHHHCCCCCC
GAQCIVVAIDAKARVGGEGWEVYTHGGRRNTGLDAVAWAQQMAEFGAGEILLTSMDRDGT
CCEEEEEEEECCEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
QIGFNLPLTRAVSDAVPVPVIASGGVGALEHLAEGIQVGGADAVLAASIFHYGTFTVGQA
EEEECCCHHHHHHCCCCCCEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEHHHH
KALMAEHGIPVRM
HHHHHHCCCCCCC
>Mature Secondary Structure
MLAKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNEQGADELTFLDITATSDGRD
CCHHHCCCCCCCCCCEEEECCCEEEEECCCCCEEEEEECCCCCCCCEEEEEEEECCCCCC
LILPIIEAVASQVFIPLTVGGGVRSVEDVRRLLNAGADKVSFNSAAVANPQLIRDASDKY
HHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHCCCCCEECCCCCCCCCHHHHCCCCCC
GAQCIVVAIDAKARVGGEGWEVYTHGGRRNTGLDAVAWAQQMAEFGAGEILLTSMDRDGT
CCEEEEEEEECCEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
QIGFNLPLTRAVSDAVPVPVIASGGVGALEHLAEGIQVGGADAVLAASIFHYGTFTVGQA
EEEECCCHHHHHHCCCCCCEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEHHHH
KALMAEHGIPVRM
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA