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The map label for this gene is nodW [H]

Identifier: 124266004

GI number: 124266004

Start: 851848

End: 852477

Strand: Reverse

Name: nodW [H]

Synonym: Mpe_A0811

Alternate gene names: 124266004

Gene position: 852477-851848 (Counterclockwise)

Preceding gene: 124266005

Following gene: 124265992

Centisome position: 21.08

GC content: 58.89

Gene sequence:

>630_bases
ATGACTGGAGGCCCTGCGCGTCCGGTCGTCTATGTCGTCGACGACGACCATGCGATGCGTGAAGCTTTGAGCAGCCTGAT
TCGATCAGCCGGCATGCGGGTCGAGGTGTTCGAATCTGCGACGACGTTTCTCAAGTCGGCATGGCACGAGGCGCCGTCAT
GCTTGGTGCTCGACGTCCGCATGCCTGAGATGAACGGGCTCGATCTGCAACGCCACATTTCCGCGTCGGGCAAGCACATT
CCCGTGATCTTCATATCCGGTCACGGCGATATCCCGATGACGGTTCGTGCGATCAAAGCGGGGGCCGTGGAGTTTCTTCC
AAAGCCGTTCTCGGACCAATGCCTCCTTGATGCCATTGCTGCAGCATTCATGGTGGAACAGCATTCTCGAAGTGAGCGTG
CTGAAGTCGAGCAAGTCCGAGAAAGCTACGGAACTCTCACCGCCAGAGAACGGGAGGTGCTGGTCGGAGTGGTCAAGGGT
CTGCGCAACAAGCAGGTCGCGTTCGATCTCGGTATCACCGAAGAGACCGTGAAGGTTCACCGTCACCGGCTCATGCAGAA
GATGAGCGCCAAGTCACTGCCTGAGCTGGTTCGCATGGTCGAGCGATTGCGGTCGAGCGCCCCGGATTAG

Upstream 100 bases:

>100_bases
AGTTCCGTCTGCCGATCGCAGAGCACGCCGAACCATGAACGAGCCCATTCGCTCACGAGCCGACGACCTGTGCTCGACCG
AAAGGCGGGAGGGCGATGGC

Downstream 100 bases:

>100_bases
TCAACGCCAGATGGCCGTTCCTCAACAGCGCACGCCAAGACGGGATGGCCGCATTCGTCAGGCGAAGCCCCACGCACTCC
CCGCTACTGCGCCGCGCGAC

Product: two-component response regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MTGGPARPVVYVVDDDHAMREALSSLIRSAGMRVEVFESATTFLKSAWHEAPSCLVLDVRMPEMNGLDLQRHISASGKHI
PVIFISGHGDIPMTVRAIKAGAVEFLPKPFSDQCLLDAIAAAFMVEQHSRSERAEVEQVRESYGTLTAREREVLVGVVKG
LRNKQVAFDLGITEETVKVHRHRLMQKMSAKSLPELVRMVERLRSSAPD

Sequences:

>Translated_209_residues
MTGGPARPVVYVVDDDHAMREALSSLIRSAGMRVEVFESATTFLKSAWHEAPSCLVLDVRMPEMNGLDLQRHISASGKHI
PVIFISGHGDIPMTVRAIKAGAVEFLPKPFSDQCLLDAIAAAFMVEQHSRSERAEVEQVRESYGTLTAREREVLVGVVKG
LRNKQVAFDLGITEETVKVHRHRLMQKMSAKSLPELVRMVERLRSSAPD
>Mature_208_residues
TGGPARPVVYVVDDDHAMREALSSLIRSAGMRVEVFESATTFLKSAWHEAPSCLVLDVRMPEMNGLDLQRHISASGKHIP
VIFISGHGDIPMTVRAIKAGAVEFLPKPFSDQCLLDAIAAAFMVEQHSRSERAEVEQVRESYGTLTAREREVLVGVVKGL
RNKQVAFDLGITEETVKVHRHRLMQKMSAKSLPELVRMVERLRSSAPD

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=104, Percent_Identity=37.5, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1788222, Length=204, Percent_Identity=23.5294117647059, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1790860, Length=105, Percent_Identity=29.5238095238095, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788521, Length=194, Percent_Identity=30.9278350515464, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1790437, Length=133, Percent_Identity=30.0751879699248, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790102, Length=194, Percent_Identity=27.319587628866, Blast_Score=60, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23186; Mature: 23055

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGGPARPVVYVVDDDHAMREALSSLIRSAGMRVEVFESATTFLKSAWHEAPSCLVLDVR
CCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCEEEEEEE
MPEMNGLDLQRHISASGKHIPVIFISGHGDIPMTVRAIKAGAVEFLPKPFSDQCLLDAIA
CCCCCCCCHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AAFMVEQHSRSERAEVEQVRESYGTLTAREREVLVGVVKGLRNKQVAFDLGITEETVKVH
HHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHH
RHRLMQKMSAKSLPELVRMVERLRSSAPD
HHHHHHHHHHCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TGGPARPVVYVVDDDHAMREALSSLIRSAGMRVEVFESATTFLKSAWHEAPSCLVLDVR
CCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCEEEEEEE
MPEMNGLDLQRHISASGKHIPVIFISGHGDIPMTVRAIKAGAVEFLPKPFSDQCLLDAIA
CCCCCCCCHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AAFMVEQHSRSERAEVEQVRESYGTLTAREREVLVGVVKGLRNKQVAFDLGITEETVKVH
HHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHH
RHRLMQKMSAKSLPELVRMVERLRSSAPD
HHHHHHHHHHCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]