Click here to switch to the map view.

The map label for this gene is gatA [H]

Identifier: 124265966

GI number: 124265966

Start: 806674

End: 808539

Strand: Reverse

Name: gatA [H]

Synonym: Mpe_A0773

Alternate gene names: 124265966

Gene position: 808539-806674 (Counterclockwise)

Preceding gene: 124265967

Following gene: 124265965

Centisome position: 19.99

GC content: 76.47

Gene sequence:

>1866_bases
ATGCACGCACCGAACCCGTTCACGCTCGAAGAGTGGCAGCAGGCCTACCGCGATGGCGCGATGCCGGGCCCGCTGCTCCA
CGAGCGGCGGCGCCAGCTGGCGGGCGCAGGGGCCGACCCGGCGTGGATCCTGCTCGCCAGCGAAGCCCAGCTCGACGACC
AACTGGCCGCGCTGGCGGCGCGCGCCGCCGTCTGCGCGGACGCGGCCGAGCGCGCCCGCGCCCTGCCGCTGTACGGCGTG
CCGTTCGCGGTCAAGGACAACATCGACATCGCCGGGCTGCCCACCACCGCCGCCTGCCCGGCGTTCTCGCACGACGCCGA
GCGCTCGGCGCACGCGGTGCAGCGGCTCGTCGAGGCCGGCGCGGTGTGGCTGGGCAAGACCAACCTCGACCAGTTCGCCA
CCGGCCTGGTCGGCACCCGCTCGCCGTATGGCCGGCCGGCGAGCGTGTTCGCCGCCGACCGCGTGAGCGGCGGCTCCAGC
TCCGGCTCGGCGGTGGTGGTCGCGCGCGGCGACGTGCCGCTCGCGCTGGGCACCGACACCGCCGGCTCGGGCCGCGTGCC
GGCCGGATTCAACAACATCGTCGGCCTCAAGCCCACACCGGGCCGCGTGTCCACCACCGGCGTGCTGCCGGCCTGCCGCT
CGCTGGACTGCGTGTCGGTGTTCGCGCACACCGTGCCGGCCGCGGCCACCGTGCTCGCGCTGATCGAAGGGCCGGACACC
GACGACGCGTACAGCGCCTTCGCGCCGGGGCCCGCCGGCTGGAACGGCCCGCTGCGCGTCGGCGTGCCGCGCGATCCGGT
CTTCAGCGGCGACGCCGGCTATGCCGCGGCCTTCGAGCGGGCGCTCGGCGAGCTGCGCCGGCTCGGCCACAGCGCGGTGC
CGATCGACTTCGCGCCGCTGCACCGGGTGGCCGACCTGCTGTACGCCGGGCCCTGGGTGGCCGAGCGCCATGCCGTGGTG
CAGGAGCTGCTCGCGCGCGACCCGCAGGCGCTGGACCCGAGCGTGCGCCAGATCGTCGAATCGGCCACCGCCTTCAACGC
CACCGATGCCTTCCGCGGCCAGTACCGGCTGCGGGCGGCGCAGCGCGACGCCACTGCGCTGTGGCGGCAGGTCGACGTGC
TGATGGTGCCCACCGCGCCGGGGCACCCGCGCTTCGACGAGGTCGACGCCGACCCGCTGGGTGCGAACGCCGGCCTGGGC
ACCTACACGAACTTCGTCAACCTGCTGGGCTGGTGCGCGCTGGCGGCGCCGGCCGGCTTCACCGCCACGCCGGTGTCCGA
AGGCCCGGCCGCCGGGCTGCCGTTCGGCGTCACCTTCATCGCTCCGGCGAACGCCGACGCCGCGCTGGCCCGCTTCGGCG
CGCAATGGCAGCGCGCGATGGATCTGCCGCTCGGCGCCTCGCGCCGCCACCTCGAAGACCTGCCCGCGGCACCGGCGGGC
CCCCTGCCGGCCGTCGAGCCCACGCTGTCGCTGGCCGTGGTGGGCGCCCATCTGTCGGGGCTGCCGCTGAACGGCCAGCT
CACCGAGCGCGGCGCGAGCCTGCTCGAGGCCACCACCACCGCGCCGCGCTACCGCCTGCACGCGCTGCCCGGCACCACGC
CGCCGAAGCCCGGCCTGGTGCGGGTCGCCGCCGGCGACGCGGGTCACGCGATCGCGGTCGAGGTGTGGGCGCTGCCGCTG
CACGCCGTCGGCTCCTTCCTCGCGCTGATCCCGCCGCCGCTGGGCCTGGGCTCGATCGAACTGGCCGACGGCCGCTGGGT
GCACGGCTTCCTGTGCGAGTCGCATGCGCTGGCCGGCGCGCCCGATGTCAGCGCCTTCGGCGGCTGGCGCGCCTATGCGG
CGTCGCTGCGCGGTGCCGCGGCCTAG

Upstream 100 bases:

>100_bases
TGGGCCACCCGCTGAACGGCCCGGCACGCCGGCGGCCCGTGGCCGGCATGGTTCCTGCTGCATCCCATCTGGTACACAAG
ATAGTGCACCAGCACACACC

Downstream 100 bases:

>100_bases
GCCCGCCGCGCCCGTCCTCATCTGCCCGGACTTCCAGGAGAACGACATGCCGCCCGCCGACACGATCGAGGCCTACGTCG
ATGCCGCCGCCGCGGCACTG

Product: urea amidolyase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 621; Mature: 621

Protein sequence:

>621_residues
MHAPNPFTLEEWQQAYRDGAMPGPLLHERRRQLAGAGADPAWILLASEAQLDDQLAALAARAAVCADAAERARALPLYGV
PFAVKDNIDIAGLPTTAACPAFSHDAERSAHAVQRLVEAGAVWLGKTNLDQFATGLVGTRSPYGRPASVFAADRVSGGSS
SGSAVVVARGDVPLALGTDTAGSGRVPAGFNNIVGLKPTPGRVSTTGVLPACRSLDCVSVFAHTVPAAATVLALIEGPDT
DDAYSAFAPGPAGWNGPLRVGVPRDPVFSGDAGYAAAFERALGELRRLGHSAVPIDFAPLHRVADLLYAGPWVAERHAVV
QELLARDPQALDPSVRQIVESATAFNATDAFRGQYRLRAAQRDATALWRQVDVLMVPTAPGHPRFDEVDADPLGANAGLG
TYTNFVNLLGWCALAAPAGFTATPVSEGPAAGLPFGVTFIAPANADAALARFGAQWQRAMDLPLGASRRHLEDLPAAPAG
PLPAVEPTLSLAVVGAHLSGLPLNGQLTERGASLLEATTTAPRYRLHALPGTTPPKPGLVRVAAGDAGHAIAVEVWALPL
HAVGSFLALIPPPLGLGSIELADGRWVHGFLCESHALAGAPDVSAFGGWRAYAASLRGAAA

Sequences:

>Translated_621_residues
MHAPNPFTLEEWQQAYRDGAMPGPLLHERRRQLAGAGADPAWILLASEAQLDDQLAALAARAAVCADAAERARALPLYGV
PFAVKDNIDIAGLPTTAACPAFSHDAERSAHAVQRLVEAGAVWLGKTNLDQFATGLVGTRSPYGRPASVFAADRVSGGSS
SGSAVVVARGDVPLALGTDTAGSGRVPAGFNNIVGLKPTPGRVSTTGVLPACRSLDCVSVFAHTVPAAATVLALIEGPDT
DDAYSAFAPGPAGWNGPLRVGVPRDPVFSGDAGYAAAFERALGELRRLGHSAVPIDFAPLHRVADLLYAGPWVAERHAVV
QELLARDPQALDPSVRQIVESATAFNATDAFRGQYRLRAAQRDATALWRQVDVLMVPTAPGHPRFDEVDADPLGANAGLG
TYTNFVNLLGWCALAAPAGFTATPVSEGPAAGLPFGVTFIAPANADAALARFGAQWQRAMDLPLGASRRHLEDLPAAPAG
PLPAVEPTLSLAVVGAHLSGLPLNGQLTERGASLLEATTTAPRYRLHALPGTTPPKPGLVRVAAGDAGHAIAVEVWALPL
HAVGSFLALIPPPLGLGSIELADGRWVHGFLCESHALAGAPDVSAFGGWRAYAASLRGAAA
>Mature_621_residues
MHAPNPFTLEEWQQAYRDGAMPGPLLHERRRQLAGAGADPAWILLASEAQLDDQLAALAARAAVCADAAERARALPLYGV
PFAVKDNIDIAGLPTTAACPAFSHDAERSAHAVQRLVEAGAVWLGKTNLDQFATGLVGTRSPYGRPASVFAADRVSGGSS
SGSAVVVARGDVPLALGTDTAGSGRVPAGFNNIVGLKPTPGRVSTTGVLPACRSLDCVSVFAHTVPAAATVLALIEGPDT
DDAYSAFAPGPAGWNGPLRVGVPRDPVFSGDAGYAAAFERALGELRRLGHSAVPIDFAPLHRVADLLYAGPWVAERHAVV
QELLARDPQALDPSVRQIVESATAFNATDAFRGQYRLRAAQRDATALWRQVDVLMVPTAPGHPRFDEVDADPLGANAGLG
TYTNFVNLLGWCALAAPAGFTATPVSEGPAAGLPFGVTFIAPANADAALARFGAQWQRAMDLPLGASRRHLEDLPAAPAG
PLPAVEPTLSLAVVGAHLSGLPLNGQLTERGASLLEATTTAPRYRLHALPGTTPPKPGLVRVAAGDAGHAIAVEVWALPL
HAVGSFLALIPPPLGLGSIELADGRWVHGFLCESHALAGAPDVSAFGGWRAYAASLRGAAA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=456, Percent_Identity=27.4122807017544, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI195972892, Length=146, Percent_Identity=33.5616438356164, Blast_Score=76, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=408, Percent_Identity=28.921568627451, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17537465, Length=147, Percent_Identity=36.0544217687075, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17556264, Length=256, Percent_Identity=27.734375, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71990152, Length=236, Percent_Identity=28.3898305084746, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538252, Length=454, Percent_Identity=22.9074889867841, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=595, Percent_Identity=43.3613445378151, Blast_Score=433, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6323950, Length=250, Percent_Identity=32.8, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648113, Length=209, Percent_Identity=37.799043062201, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24652985, Length=427, Percent_Identity=25.0585480093677, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922090, Length=427, Percent_Identity=25.0585480093677, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24652981, Length=427, Percent_Identity=25.0585480093677, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24652983, Length=427, Percent_Identity=25.0585480093677, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356731, Length=140, Percent_Identity=33.5714285714286, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 64129; Mature: 64129

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAPNPFTLEEWQQAYRDGAMPGPLLHERRRQLAGAGADPAWILLASEAQLDDQLAALAA
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHH
RAAVCADAAERARALPLYGVPFAVKDNIDIAGLPTTAACPAFSHDAERSAHAVQRLVEAG
HHHHHHHHHHHHCCCCEECCCEEEECCCEEECCCCHHCCCCCCCHHHHHHHHHHHHHHHC
AVWLGKTNLDQFATGLVGTRSPYGRPASVFAADRVSGGSSSGSAVVVARGDVPLALGTDT
CEEECCCCHHHHHHHCCCCCCCCCCCHHEEEHHCCCCCCCCCCEEEEEECCEEEEECCCC
AGSGRVPAGFNNIVGLKPTPGRVSTTGVLPACRSLDCVSVFAHTVPAAATVLALIEGPDT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHEECCCCC
DDAYSAFAPGPAGWNGPLRVGVPRDPVFSGDAGYAAAFERALGELRRLGHSAVPIDFAPL
CCCHHCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHH
HRVADLLYAGPWVAERHAVVQELLARDPQALDPSVRQIVESATAFNATDAFRGQYRLRAA
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHCCHHHHHHH
QRDATALWRQVDVLMVPTAPGHPRFDEVDADPLGANAGLGTYTNFVNLLGWCALAAPAGF
HHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
TATPVSEGPAAGLPFGVTFIAPANADAALARFGAQWQRAMDLPLGASRRHLEDLPAAPAG
CCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCC
PLPAVEPTLSLAVVGAHLSGLPLNGQLTERGASLLEATTTAPRYRLHALPGTTPPKPGLV
CCCCCCCCCEEEEEEHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEE
RVAAGDAGHAIAVEVWALPLHAVGSFLALIPPPLGLGSIELADGRWVHGFLCESHALAGA
EEECCCCCCEEEEEEEHHHHHHHHHHHHHCCCCCCCCCEEECCCCEEEEEEECCCCCCCC
PDVSAFGGWRAYAASLRGAAA
CCCHHCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MHAPNPFTLEEWQQAYRDGAMPGPLLHERRRQLAGAGADPAWILLASEAQLDDQLAALAA
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHH
RAAVCADAAERARALPLYGVPFAVKDNIDIAGLPTTAACPAFSHDAERSAHAVQRLVEAG
HHHHHHHHHHHHCCCCEECCCEEEECCCEEECCCCHHCCCCCCCHHHHHHHHHHHHHHHC
AVWLGKTNLDQFATGLVGTRSPYGRPASVFAADRVSGGSSSGSAVVVARGDVPLALGTDT
CEEECCCCHHHHHHHCCCCCCCCCCCHHEEEHHCCCCCCCCCCEEEEEECCEEEEECCCC
AGSGRVPAGFNNIVGLKPTPGRVSTTGVLPACRSLDCVSVFAHTVPAAATVLALIEGPDT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHEECCCCC
DDAYSAFAPGPAGWNGPLRVGVPRDPVFSGDAGYAAAFERALGELRRLGHSAVPIDFAPL
CCCHHCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHH
HRVADLLYAGPWVAERHAVVQELLARDPQALDPSVRQIVESATAFNATDAFRGQYRLRAA
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHCCHHHHHHH
QRDATALWRQVDVLMVPTAPGHPRFDEVDADPLGANAGLGTYTNFVNLLGWCALAAPAGF
HHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
TATPVSEGPAAGLPFGVTFIAPANADAALARFGAQWQRAMDLPLGASRRHLEDLPAAPAG
CCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCC
PLPAVEPTLSLAVVGAHLSGLPLNGQLTERGASLLEATTTAPRYRLHALPGTTPPKPGLV
CCCCCCCCCEEEEEEHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEE
RVAAGDAGHAIAVEVWALPLHAVGSFLALIPPPLGLGSIELADGRWVHGFLCESHALAGA
EEECCCCCCEEEEEEEHHHHHHHHHHHHHCCCCCCCCCEEECCCCEEEEEEECCCCCCCC
PDVSAFGGWRAYAASLRGAAA
CCCHHCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA