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The map label for this gene is gatA [H]

Identifier: 124265965

GI number: 124265965

Start: 805003

End: 806421

Strand: Reverse

Name: gatA [H]

Synonym: Mpe_A0772

Alternate gene names: 124265965

Gene position: 806421-805003 (Counterclockwise)

Preceding gene: 124265966

Following gene: 124265964

Centisome position: 19.94

GC content: 76.46

Gene sequence:

>1419_bases
GTGAGCGCCGACACCCTGCTCGCCGGCACCGCCACCGGCATCGCTGCCGCGGTGCAGCGCGGCGAGGTGAGCGCCAGTGC
GATGGTGCAGGCCAGCCTCGCGCGCATCACCGCCACCGACGCACGCGTCAATGCCTTCACCGACCTCACCGCGCAGCGCG
CGCTGGAGCGTGCCGCCACGCTCGACGCGCGCCACGCGGCCGGCGACACCGCGGCACTGCCGCTGCTGGGCGTGCCCTTC
GCGGTGAAGAACCTGTTCGACGTGCAGGGCCTGGCCACGCTGGCCGGCTCGAAGATCGAGCGCGAGCGCGCGCCGGCGCT
GCGCGACGGGCCGCTGGTCGAGCGGCTCGAGGCCGCCGGTGCCGTGCTGGTGGGCGCGTTGAACATGGACGAGTACGCCT
ACGGCTTCACCACCGAGAACAGCCATGCCGGCGCCACCCACAACCCGCACGACCTGGCGCGCAGCGCGGGCGGCTCGTCG
GGCGGCTCGGGCGCGGCCATCGCCGCCGGCCAGGTGCCGCTGACCCTGGGCTCCGACACCAACGGATCGATCCGCGTGCC
CAGTTCGCTGTGCGGGGTGTTCGGCCTCAAGCCCACCTACGGCCGGCTGCCGCGCCACGGCTCCTACCCCTTCGTCGCCA
GCCTCGACCACCTCGGGCCGTTCGCGCGCTCGGTGCCCGACCTGGCGCTCAGCTACGACCTGCTGCAGGGCTGGGAGGCC
GCCGACCCGGGCTGCGTGCAGCGCCCCATCGAGCCGACGCACGCCACGCTGGCGCGGGGCGCGGGCGGCCTGCGCATCGC
GGTGCTCGGCGGCTACTTCCACGAGCATGCGCTGCCCGAGGCGCGCGCCGCGGTCGAGCGTGTCGCGGCGGCGCTCGGCG
TCACGCGCCGCGTCGAGCTGCCGGAGGTGGCACGCGCCCGCGCCGCCGCCTTCCTGATCAGCAACGCCGAGTGCGCCACG
CTGCACCTGGGCGACCTGCGCACGCGCGCGCAGGACTTCGAGCCGCTGTCGCGCGACCGCTTCCTCGCCGGCGCGCTGCT
GCCGGCCGCCTGGGTGCTGCAGGCGCAGCGCGTGCGGCGCTGGTTCGCGCAGCGCGCGGCCGAGCTGTTCGGCCAGGTCG
ACGTGCTGCTGGCACCGGCCACGCCCTGCGTCGCGCCGCGGCTCGGCACCGAGTGGCTGGAGCTGAACGGCCAGCGGCTC
CCGGCCCGGCCCAGCCTGGGCCTGCTGACGCAGCCGATCTCCTGCATCGGCCTGCCGGTGTGCGCAGTGCCGGTGTGGGG
CTGCAGCGAGGCGCAGCCGGGCCTGCCGATCGGCGTGCAGGTGATCGCCGCGCCCTGGCGCGAGGACCACGCGCTGCGTG
TCGGCGCCGCGCTCGAGGCCGCCGGCGTGGTGCGCTCGCCGGTCGCCGCGCTCGACTGA

Upstream 100 bases:

>100_bases
TCGCGTCGCAGATGGCCGCGCTGGTGAACGGCCTGCCGCTCGCCGTGGAGGACGAGCCGGCACCGCAGTTCGTGCCGCTG
TCGCCGCAGGACGCCGCGCC

Downstream 100 bases:

>100_bases
ATCCGAAGGAAGATCGGCGATGACCGAAGACCACGACATCAACCGCCCCGAGGTGCTGGCCGAGATGCAGGCCGTGTTCG
CGCGCTACGAGGACGCGCTG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSADTLLAGTATGIAAAVQRGEVSASAMVQASLARITATDARVNAFTDLTAQRALERAATLDARHAAGDTAALPLLGVPF
AVKNLFDVQGLATLAGSKIERERAPALRDGPLVERLEAAGAVLVGALNMDEYAYGFTTENSHAGATHNPHDLARSAGGSS
GGSGAAIAAGQVPLTLGSDTNGSIRVPSSLCGVFGLKPTYGRLPRHGSYPFVASLDHLGPFARSVPDLALSYDLLQGWEA
ADPGCVQRPIEPTHATLARGAGGLRIAVLGGYFHEHALPEARAAVERVAAALGVTRRVELPEVARARAAAFLISNAECAT
LHLGDLRTRAQDFEPLSRDRFLAGALLPAAWVLQAQRVRRWFAQRAAELFGQVDVLLAPATPCVAPRLGTEWLELNGQRL
PARPSLGLLTQPISCIGLPVCAVPVWGCSEAQPGLPIGVQVIAAPWREDHALRVGAALEAAGVVRSPVAALD

Sequences:

>Translated_472_residues
MSADTLLAGTATGIAAAVQRGEVSASAMVQASLARITATDARVNAFTDLTAQRALERAATLDARHAAGDTAALPLLGVPF
AVKNLFDVQGLATLAGSKIERERAPALRDGPLVERLEAAGAVLVGALNMDEYAYGFTTENSHAGATHNPHDLARSAGGSS
GGSGAAIAAGQVPLTLGSDTNGSIRVPSSLCGVFGLKPTYGRLPRHGSYPFVASLDHLGPFARSVPDLALSYDLLQGWEA
ADPGCVQRPIEPTHATLARGAGGLRIAVLGGYFHEHALPEARAAVERVAAALGVTRRVELPEVARARAAAFLISNAECAT
LHLGDLRTRAQDFEPLSRDRFLAGALLPAAWVLQAQRVRRWFAQRAAELFGQVDVLLAPATPCVAPRLGTEWLELNGQRL
PARPSLGLLTQPISCIGLPVCAVPVWGCSEAQPGLPIGVQVIAAPWREDHALRVGAALEAAGVVRSPVAALD
>Mature_471_residues
SADTLLAGTATGIAAAVQRGEVSASAMVQASLARITATDARVNAFTDLTAQRALERAATLDARHAAGDTAALPLLGVPFA
VKNLFDVQGLATLAGSKIERERAPALRDGPLVERLEAAGAVLVGALNMDEYAYGFTTENSHAGATHNPHDLARSAGGSSG
GSGAAIAAGQVPLTLGSDTNGSIRVPSSLCGVFGLKPTYGRLPRHGSYPFVASLDHLGPFARSVPDLALSYDLLQGWEAA
DPGCVQRPIEPTHATLARGAGGLRIAVLGGYFHEHALPEARAAVERVAAALGVTRRVELPEVARARAAAFLISNAECATL
HLGDLRTRAQDFEPLSRDRFLAGALLPAAWVLQAQRVRRWFAQRAAELFGQVDVLLAPATPCVAPRLGTEWLELNGQRLP
ARPSLGLLTQPISCIGLPVCAVPVWGCSEAQPGLPIGVQVIAAPWREDHALRVGAALEAAGVVRSPVAALD

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=517, Percent_Identity=27.0793036750484, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI195972892, Length=245, Percent_Identity=32.6530612244898, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI166795287, Length=460, Percent_Identity=29.5652173913043, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=440, Percent_Identity=27.9545454545455, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17537465, Length=494, Percent_Identity=26.7206477732794, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17556264, Length=229, Percent_Identity=31.8777292576419, Blast_Score=104, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17538252, Length=513, Percent_Identity=24.5614035087719, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71990152, Length=507, Percent_Identity=24.0631163708087, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=203, Percent_Identity=31.0344827586207, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=201, Percent_Identity=31.3432835820896, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=501, Percent_Identity=25.1497005988024, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=155, Percent_Identity=42.5806451612903, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323950, Length=251, Percent_Identity=26.2948207171315, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320448, Length=255, Percent_Identity=29.8039215686275, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648113, Length=461, Percent_Identity=32.1041214750542, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24644968, Length=504, Percent_Identity=26.5873015873016, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24652985, Length=496, Percent_Identity=27.4193548387097, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI19922090, Length=496, Percent_Identity=27.4193548387097, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24652981, Length=496, Percent_Identity=27.4193548387097, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24652983, Length=496, Percent_Identity=27.4193548387097, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI161078093, Length=501, Percent_Identity=28.7425149700599, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24648435, Length=515, Percent_Identity=24.4660194174757, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24648437, Length=515, Percent_Identity=24.4660194174757, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI21356731, Length=238, Percent_Identity=33.1932773109244, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI45550774, Length=515, Percent_Identity=24.4660194174757, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24648441, Length=441, Percent_Identity=24.9433106575964, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648439, Length=441, Percent_Identity=24.9433106575964, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49087; Mature: 48956

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADTLLAGTATGIAAAVQRGEVSASAMVQASLARITATDARVNAFTDLTAQRALERAAT
CCCCCEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHH
LDARHAAGDTAALPLLGVPFAVKNLFDVQGLATLAGSKIERERAPALRDGPLVERLEAAG
HHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHCC
AVLVGALNMDEYAYGFTTENSHAGATHNPHDLARSAGGSSGGSGAAIAAGQVPLTLGSDT
EEEEEEECCCCCEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEECCEEEEECCCC
NGSIRVPSSLCGVFGLKPTYGRLPRHGSYPFVASLDHLGPFARSVPDLALSYDLLQGWEA
CCCEECCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCHHHHHCHHHHHHHHHHHCCCC
ADPGCVQRPIEPTHATLARGAGGLRIAVLGGYFHEHALPEARAAVERVAAALGVTRRVEL
CCCCHHCCCCCCHHHHHHCCCCCEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCHHCCCC
PEVARARAAAFLISNAECATLHLGDLRTRAQDFEPLSRDRFLAGALLPAAWVLQAQRVRR
HHHHHHHHHHHEECCCCEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WFAQRAAELFGQVDVLLAPATPCVAPRLGTEWLELNGQRLPARPSLGLLTQPISCIGLPV
HHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHCCHHHHCCCH
CAVPVWGCSEAQPGLPIGVQVIAAPWREDHALRVGAALEAAGVVRSPVAALD
HHCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEECHHHHHHHHHHCCHHHCC
>Mature Secondary Structure 
SADTLLAGTATGIAAAVQRGEVSASAMVQASLARITATDARVNAFTDLTAQRALERAAT
CCCCEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHH
LDARHAAGDTAALPLLGVPFAVKNLFDVQGLATLAGSKIERERAPALRDGPLVERLEAAG
HHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHCC
AVLVGALNMDEYAYGFTTENSHAGATHNPHDLARSAGGSSGGSGAAIAAGQVPLTLGSDT
EEEEEEECCCCCEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEECCEEEEECCCC
NGSIRVPSSLCGVFGLKPTYGRLPRHGSYPFVASLDHLGPFARSVPDLALSYDLLQGWEA
CCCEECCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCHHHHHCHHHHHHHHHHHCCCC
ADPGCVQRPIEPTHATLARGAGGLRIAVLGGYFHEHALPEARAAVERVAAALGVTRRVEL
CCCCHHCCCCCCHHHHHHCCCCCEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCHHCCCC
PEVARARAAAFLISNAECATLHLGDLRTRAQDFEPLSRDRFLAGALLPAAWVLQAQRVRR
HHHHHHHHHHHEECCCCEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WFAQRAAELFGQVDVLLAPATPCVAPRLGTEWLELNGQRLPARPSLGLLTQPISCIGLPV
HHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHCCHHHHCCCH
CAVPVWGCSEAQPGLPIGVQVIAAPWREDHALRVGAALEAAGVVRSPVAALD
HHCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEECHHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA