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The map label for this gene is pyrB

Identifier: 124265901

GI number: 124265901

Start: 737427

End: 738389

Strand: Direct

Name: pyrB

Synonym: Mpe_A0708

Alternate gene names: 124265901

Gene position: 737427-738389 (Clockwise)

Preceding gene: 124265900

Following gene: 124265902

Centisome position: 18.23

GC content: 67.29

Gene sequence:

>963_bases
ATGCTGAACAAGCGCAACCCGCAGCTCAACGCCCACGGCGAGCTGATCCACCTGCTGTCGATCGAAGGCCTGCCGAAGGC
GGTGCTGACGCAGATCCTCGACACCGCGGGCACCTTCCTGAGCGTCAACGACCGCGAGGTGAAGAAGGTGCCGCTGCTGC
GGGGCAAGAGCGTGTTCAACCTGTTCTTCGAGAACAGCACGCGCACGCGCACCACCTTCGAGATCGCTGCCAAGCGCCTC
AGCGCCGACGTCATCAACCTCGACATCGCGCGCAGTTCCACCGCCAAGGGCGAGAGCCTGCTCGACACCATCGCCAACCT
GTCGGCGATGCACGCCGACATGTTCGTGGTGCGCCATGCCGAAAGCGGCGCGCCCTACCTGATCGCGCAGCACGTGGCGC
CCCACGTGCACGTGGTCAACGCCGGCGACGGCCGTCACGCGCACCCCACGCAGGGGCTGCTCGACATGTACACGATCCGC
CACTACAAGAAGGACTTCAGCAACCTCGTGGTGGCGATCGTCGGCGACATCGTGCACTCGCGCGTGGCGCGCTCCGACAT
CCACGCGCTCACCACGCTGGGCGCGGCGGAGGTGAGGGCGGTCGGCCCGCGCACCCTCGTGCCGGCCGACCTGAAGCACA
TGGGCGTGCGCGTCTGCCACGACATGAGCGAAGGCATCCGCGACGCCGACGTGATCATCATGCTGCGGCTGCAGAACGAG
CGCATGAGCGGTGCGCTGCTGCCGAGCGCCGGCGAGTACTTCAAGAGCTTCGGCCTCACCGAGGAGAAGCTCGCGCTCGC
CAGGCCCGACGCCATCGTGATGCACCCCGGCCCCATCAACCGCGGCGTGGAGATCGACTCGTCGGTGGTCGACGGCAGGC
AGAGCGTGATCCTGCCGCAGGTGACCTTCGGCATCGCGGTGCGCATGGCGGTCATGAGCACGATCGCGGGGAACAACGCA
TGA

Upstream 100 bases:

>100_bases
CCGCCGTGTCGGCCGCCCGCGTGGTGGTGCCGCGCCACCAGCGGCTCGCGCTGGTGCGCGACGAGGCCGGCGGCTTCACA
CTCCAACTCGAAAGCGCCTG

Downstream 100 bases:

>100_bases
AGATCCTGATCAGCAACGGCCGCGTGGTCGACCCGGCCTCGGGCCGCGACGAGCGGGCCGACATCGCCATCGCCAACGGC
CGCATCCTCACGATCGGCAA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MLNKRNPQLNAHGELIHLLSIEGLPKAVLTQILDTAGTFLSVNDREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAAKRL
SADVINLDIARSSTAKGESLLDTIANLSAMHADMFVVRHAESGAPYLIAQHVAPHVHVVNAGDGRHAHPTQGLLDMYTIR
HYKKDFSNLVVAIVGDIVHSRVARSDIHALTTLGAAEVRAVGPRTLVPADLKHMGVRVCHDMSEGIRDADVIIMLRLQNE
RMSGALLPSAGEYFKSFGLTEEKLALARPDAIVMHPGPINRGVEIDSSVVDGRQSVILPQVTFGIAVRMAVMSTIAGNNA

Sequences:

>Translated_320_residues
MLNKRNPQLNAHGELIHLLSIEGLPKAVLTQILDTAGTFLSVNDREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAAKRL
SADVINLDIARSSTAKGESLLDTIANLSAMHADMFVVRHAESGAPYLIAQHVAPHVHVVNAGDGRHAHPTQGLLDMYTIR
HYKKDFSNLVVAIVGDIVHSRVARSDIHALTTLGAAEVRAVGPRTLVPADLKHMGVRVCHDMSEGIRDADVIIMLRLQNE
RMSGALLPSAGEYFKSFGLTEEKLALARPDAIVMHPGPINRGVEIDSSVVDGRQSVILPQVTFGIAVRMAVMSTIAGNNA
>Mature_320_residues
MLNKRNPQLNAHGELIHLLSIEGLPKAVLTQILDTAGTFLSVNDREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAAKRL
SADVINLDIARSSTAKGESLLDTIANLSAMHADMFVVRHAESGAPYLIAQHVAPHVHVVNAGDGRHAHPTQGLLDMYTIR
HYKKDFSNLVVAIVGDIVHSRVARSDIHALTTLGAAEVRAVGPRTLVPADLKHMGVRVCHDMSEGIRDADVIIMLRLQNE
RMSGALLPSAGEYFKSFGLTEEKLALARPDAIVMHPGPINRGVEIDSSVVDGRQSVILPQVTFGIAVRMAVMSTIAGNNA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=313, Percent_Identity=34.5047923322684, Blast_Score=149, Evalue=3e-36,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=33.6569579288026, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1786469, Length=229, Percent_Identity=28.3842794759825, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=333, Percent_Identity=31.8318318318318, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6322331, Length=313, Percent_Identity=30.3514376996805, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24642586, Length=313, Percent_Identity=33.2268370607029, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_METPP (A2SDN1)

Other databases:

- EMBL:   CP000555
- RefSeq:   YP_001019905.1
- ProteinModelPortal:   A2SDN1
- SMR:   A2SDN1
- STRING:   A2SDN1
- GeneID:   4783893
- GenomeReviews:   CP000555_GR
- KEGG:   mpt:Mpe_A0708
- NMPDR:   fig|279263.3.peg.3268
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   MPET420662:MPE_A0708-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34760; Mature: 34760

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNKRNPQLNAHGELIHLLSIEGLPKAVLTQILDTAGTFLSVNDREVKKVPLLRGKSVFN
CCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEECCCCHHHHCCCCCCCCEEE
LFFENSTRTRTTFEIAAKRLSADVINLDIARSSTAKGESLLDTIANLSAMHADMFVVRHA
EEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEEEEC
ESGAPYLIAQHVAPHVHVVNAGDGRHAHPTQGLLDMYTIRHYKKDFSNLVVAIVGDIVHS
CCCCCEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVARSDIHALTTLGAAEVRAVGPRTLVPADLKHMGVRVCHDMSEGIRDADVIIMLRLQNE
HHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
RMSGALLPSAGEYFKSFGLTEEKLALARPDAIVMHPGPINRGVEIDSSVVDGRQSVILPQ
CCCCCCCCCHHHHHHHHCCCHHHHHHCCCCEEEECCCCCCCCCCCCHHHHCCCCCEEEHH
VTFGIAVRMAVMSTIAGNNA
HHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLNKRNPQLNAHGELIHLLSIEGLPKAVLTQILDTAGTFLSVNDREVKKVPLLRGKSVFN
CCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEECCCCHHHHCCCCCCCCEEE
LFFENSTRTRTTFEIAAKRLSADVINLDIARSSTAKGESLLDTIANLSAMHADMFVVRHA
EEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEEEEC
ESGAPYLIAQHVAPHVHVVNAGDGRHAHPTQGLLDMYTIRHYKKDFSNLVVAIVGDIVHS
CCCCCEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVARSDIHALTTLGAAEVRAVGPRTLVPADLKHMGVRVCHDMSEGIRDADVIIMLRLQNE
HHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
RMSGALLPSAGEYFKSFGLTEEKLALARPDAIVMHPGPINRGVEIDSSVVDGRQSVILPQ
CCCCCCCCCHHHHHHHHCCCHHHHHHCCCCEEEECCCCCCCCCCCCHHHHCCCCCEEEHH
VTFGIAVRMAVMSTIAGNNA
HHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA