Click here to switch to the map view.

The map label for this gene is pyrC' [H]

Identifier: 124265902

GI number: 124265902

Start: 738386

End: 739705

Strand: Direct

Name: pyrC' [H]

Synonym: Mpe_A0709

Alternate gene names: 124265902

Gene position: 738386-739705 (Clockwise)

Preceding gene: 124265901

Following gene: 124265903

Centisome position: 18.26

GC content: 74.77

Gene sequence:

>1320_bases
ATGAAGATCCTGATCAGCAACGGCCGCGTGGTCGACCCGGCCTCGGGCCGCGACGAGCGGGCCGACATCGCCATCGCCAA
CGGCCGCATCCTCACGATCGGCAAGGTGCCGGCCGACTTCGCGCCGGAGCGCACCATCGACGCCGCCGGCCTGGTGGTGG
CGCCCGGCCTGGTGGACCTGGCCGCGCGCCTGCGTGAGCCCGGCCACGAGCACGAGGGCATGCTCGAGAGCGAGCTGGCC
GCCGCCGCCGCCGGTGGCGTGACCAGCCTGGTCTGCCTGCCCGACACCGACCCCACGCTCGACGAGCCCGGGCTGGTGGA
GATGCTCAAGTTCCGCGCCCGCAAGCTCAGCCGCTGCCGGCTGTTCCCGCTCGGCGCGCTGACGCGCGGCCTCGGCGGCG
AGGTGCTGACCGAGATGGCCGAGCTCACCGAGGCCGGCTGCGTGGGCTTCTCGCAGGCCGACGCGGTGCTGCGCGACACG
CAGGTGCTGTCGCGCGCGCTGCAGTACGCGGCCACCTTCGGCTACACGGTGTGGCTGCGGCCGAACGATCCCTGGCTGGG
CTCGGGCGTCGCCGCCAGCGGCGCGGTCGCCACGCGGCTCGGGTTGTCGGGCGTGCCGGTGGCGGCCGAGACGATCGCGC
TGCACACGCTGTTCGAGCTGGTGCGCTCCAGCGGCGCGCGCGTGCACCTGTGCCGCTTGTCCAGCGCTGCCGGCGTGGCG
CTGCTGCGCGAGGCCAAGGCCGAGGGGCTGCCGGTCACGGCCGATGTCAGCATCAACTCGCTGCACCTGACCGATGTCGA
CATCGGCTACTTCAACCCGGCCATGCGCCTGGTGCCGCCGCTGCGCCAGCAGCGCGACCGCGACGCGCTGCGCGCCGGGC
TGGCCGACGGCACGATCGACGCGCTGGTGTCCGACCACACGCCGGTCGACGAGGACGCCAAGACCCTGCCGTTCGCCGAG
GCCGAACCCGGTGCCACCGGCCTGGAGCTGCTGCTGAGCCTGGCGCTCAAGTGGGGCGAGGACAGCGGCGCCGGCCTGCG
CCGCGCGCTCGCCACCGTGAGCTGCGAGCCGGTGCGGGTGCTGGGCGACGCGCTCGGTTCGCTCGCCTCCAGCGCGGGCC
GCCTCGTCGAGGGCGGGGTGGCCGATGTCTGCGTGTTCGACCCCGAGGCGCGCTGGACCGTCGCGCCGGCCGCACTGCGC
AGCCAGGGCAAGCACACGCCGTTCGCTTTCGACACCAGCGGCTTCGCGCTGCCGGGGCGCGTGCGCGCCACCTTGGTCGC
CGGCCACGTGGCGCACGAAGCCACGCGCGACGCGGCATGA

Upstream 100 bases:

>100_bases
CGTCGGTGGTCGACGGCAGGCAGAGCGTGATCCTGCCGCAGGTGACCTTCGGCATCGCGGTGCGCATGGCGGTCATGAGC
ACGATCGCGGGGAACAACGC

Downstream 100 bases:

>100_bases
GCGCCCGTCGCGTCACCGCCGCGCCACCCTGATGCTGCGCCGTCTGCGTGCCGCGTGGCGCCTGCTGCGCGTGCTGCTGC
ACGTGTTGCACGGCCTGGCC

Product: dihydroorotase

Products: N-carbamoyl-L-aspartate

Alternate protein names: Aspartate carbamoyltransferase 44 kDa Non-catalytic chain [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MKILISNGRVVDPASGRDERADIAIANGRILTIGKVPADFAPERTIDAAGLVVAPGLVDLAARLREPGHEHEGMLESELA
AAAAGGVTSLVCLPDTDPTLDEPGLVEMLKFRARKLSRCRLFPLGALTRGLGGEVLTEMAELTEAGCVGFSQADAVLRDT
QVLSRALQYAATFGYTVWLRPNDPWLGSGVAASGAVATRLGLSGVPVAAETIALHTLFELVRSSGARVHLCRLSSAAGVA
LLREAKAEGLPVTADVSINSLHLTDVDIGYFNPAMRLVPPLRQQRDRDALRAGLADGTIDALVSDHTPVDEDAKTLPFAE
AEPGATGLELLLSLALKWGEDSGAGLRRALATVSCEPVRVLGDALGSLASSAGRLVEGGVADVCVFDPEARWTVAPAALR
SQGKHTPFAFDTSGFALPGRVRATLVAGHVAHEATRDAA

Sequences:

>Translated_439_residues
MKILISNGRVVDPASGRDERADIAIANGRILTIGKVPADFAPERTIDAAGLVVAPGLVDLAARLREPGHEHEGMLESELA
AAAAGGVTSLVCLPDTDPTLDEPGLVEMLKFRARKLSRCRLFPLGALTRGLGGEVLTEMAELTEAGCVGFSQADAVLRDT
QVLSRALQYAATFGYTVWLRPNDPWLGSGVAASGAVATRLGLSGVPVAAETIALHTLFELVRSSGARVHLCRLSSAAGVA
LLREAKAEGLPVTADVSINSLHLTDVDIGYFNPAMRLVPPLRQQRDRDALRAGLADGTIDALVSDHTPVDEDAKTLPFAE
AEPGATGLELLLSLALKWGEDSGAGLRRALATVSCEPVRVLGDALGSLASSAGRLVEGGVADVCVFDPEARWTVAPAALR
SQGKHTPFAFDTSGFALPGRVRATLVAGHVAHEATRDAA
>Mature_439_residues
MKILISNGRVVDPASGRDERADIAIANGRILTIGKVPADFAPERTIDAAGLVVAPGLVDLAARLREPGHEHEGMLESELA
AAAAGGVTSLVCLPDTDPTLDEPGLVEMLKFRARKLSRCRLFPLGALTRGLGGEVLTEMAELTEAGCVGFSQADAVLRDT
QVLSRALQYAATFGYTVWLRPNDPWLGSGVAASGAVATRLGLSGVPVAAETIALHTLFELVRSSGARVHLCRLSSAAGVA
LLREAKAEGLPVTADVSINSLHLTDVDIGYFNPAMRLVPPLRQQRDRDALRAGLADGTIDALVSDHTPVDEDAKTLPFAE
AEPGATGLELLLSLALKWGEDSGAGLRRALATVSCEPVRVLGDALGSLASSAGRLVEGGVADVCVFDPEARWTVAPAALR
SQGKHTPFAFDTSGFALPGRVRATLVAGHVAHEATRDAA

Specific function: Non-functional DHOase [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. PyrC' subfamily [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=397, Percent_Identity=28.463476070529, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI19923821, Length=439, Percent_Identity=26.4236902050114, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1786722, Length=448, Percent_Identity=26.1160714285714, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI87082175, Length=455, Percent_Identity=25.4945054945055, Blast_Score=89, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6322218, Length=406, Percent_Identity=23.8916256157635, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI18859883, Length=431, Percent_Identity=28.0742459396752, Blast_Score=89, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004722
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.5.2.3

Molecular weight: Translated: 45781; Mature: 45781

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILISNGRVVDPASGRDERADIAIANGRILTIGKVPADFAPERTIDAAGLVVAPGLVDL
CEEEECCCEEECCCCCCCCCCCEEEECCEEEEEECCCCCCCCCCCCCCCCEEECCCHHHH
AARLREPGHEHEGMLESELAAAAAGGVTSLVCLPDTDPTLDEPGLVEMLKFRARKLSRCR
HHHHHCCCCHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCE
LFPLGALTRGLGGEVLTEMAELTEAGCVGFSQADAVLRDTQVLSRALQYAATFGYTVWLR
ECCHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
PNDPWLGSGVAASGAVATRLGLSGVPVAAETIALHTLFELVRSSGARVHLCRLSSAAGVA
CCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHH
LLREAKAEGLPVTADVSINSLHLTDVDIGYFNPAMRLVPPLRQQRDRDALRAGLADGTID
HHHHHHCCCCCEEEECEECEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCHHH
ALVSDHTPVDEDAKTLPFAEAEPGATGLELLLSLALKWGEDSGAGLRRALATVSCEPVRV
HHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHH
LGDALGSLASSAGRLVEGGVADVCVFDPEARWTVAPAALRSQGKHTPFAFDTSGFALPGR
HHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEECHHHHHCCCCCCCEEECCCCCCCCCH
VRATLVAGHVAHEATRDAA
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKILISNGRVVDPASGRDERADIAIANGRILTIGKVPADFAPERTIDAAGLVVAPGLVDL
CEEEECCCEEECCCCCCCCCCCEEEECCEEEEEECCCCCCCCCCCCCCCCEEECCCHHHH
AARLREPGHEHEGMLESELAAAAAGGVTSLVCLPDTDPTLDEPGLVEMLKFRARKLSRCR
HHHHHCCCCHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCE
LFPLGALTRGLGGEVLTEMAELTEAGCVGFSQADAVLRDTQVLSRALQYAATFGYTVWLR
ECCHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
PNDPWLGSGVAASGAVATRLGLSGVPVAAETIALHTLFELVRSSGARVHLCRLSSAAGVA
CCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHH
LLREAKAEGLPVTADVSINSLHLTDVDIGYFNPAMRLVPPLRQQRDRDALRAGLADGTID
HHHHHHCCCCCEEEECEECEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCHHH
ALVSDHTPVDEDAKTLPFAEAEPGATGLELLLSLALKWGEDSGAGLRRALATVSCEPVRV
HHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHH
LGDALGSLASSAGRLVEGGVADVCVFDPEARWTVAPAALRSQGKHTPFAFDTSGFALPGR
HHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEECHHHHHCCCCCCCEEECCCCCCCCCH
VRATLVAGHVAHEATRDAA
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-dihydroorotate; H2O

Specific reaction: (S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]