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The map label for this gene is phrB [H]

Identifier: 124265897

GI number: 124265897

Start: 734397

End: 735890

Strand: Reverse

Name: phrB [H]

Synonym: Mpe_A0704

Alternate gene names: 124265897

Gene position: 735890-734397 (Counterclockwise)

Preceding gene: 124265906

Following gene: 124265888

Centisome position: 18.2

GC content: 71.22

Gene sequence:

>1494_bases
ATGGACAAGGCCTCCGGAAAAACACTCGACGCCGCGCTGGTGTGGTGCCGCCGGGACCTGCGCGCCCACGACCATGCCGC
GCTCTACCACGCGCTGCGCGCCGCACGGCGGGTCTGGGTGGCATTCGTGTTCGACACCGAGATCCTGGACCCGCTGCCGC
GCACCGACCGCCGCGTCGAGTTCATCCGCGACAGCCTGGTCGACCTGGACGCGCAGCTGCACACCCTGGGCCGCAGCCAC
GGCACCGAGGGCGTCGGGCTGATCGTGCGCCATGGCCGCGCGCGCGACGAGATCCCGCGACTGGCCGCCGCACTGCACGT
GCAGGCGGTCTACGCGAACACCGACTACGAGCCCGCCGCCGTGGCGCGCGACGCCCAGGTGCGAGGCGCGCTCGCCCAGA
CCGGGATCGCCTTCCACCCGGCCAAGGACCAGGTGGTGTTCGACACCGACGAGGTGCTGACTGGCGCCGGCACGCCCTAT
GGCGTGTTCACGCCCTACAAGAACGCCTGGCTGAAGAAGCTGGAGCCCTTCTACCTGCAGGCCTACCCGGTGGGCCGCCA
TGCCGGCGCGCTGGCGCCGCGCCCGGCGGGTGAGGCCGCGGGCGTGCCCACGCTGGCCGCCATCGGCTTCGAACCCACCA
ACCTGGCGGCGCTGAAGCTCGCCCCCGGTACCGCCGGCGGCCGCGCGCTGCTGGAGGACTTCGGCGACGAGCGCATCGAT
CGCTACGACCAGACGCGCGACTTCCCCGCCGTCAAGGGCCCCAGCTACCTGAGCGTGCACCTGCGCTTCGGCACGCTGTC
GATCCGCGAGTTGGCGCGGCTGGCCTGGGACCGCGCGCGCGCCGGCTCGCGCGGCGCCGAGGTCTGGCTGTCGGAGCTGA
TCTGGCGCGACTTCTACCACCAGGTGCTGCACCACCACCCACAGGTCGTCGAGCGCAGCTTCAAGCCCGAATACGACAAG
ATCAAGTGGGAGCATGGCAAGCATGCCGACGCGCTGTTCGCCGCCTGGTGCGAGGGCCGCACCGGCTACCCGCTGGTCGA
CGCGGCCATGCTCCAGATCAACCAGACCGGCTACATGCACAACCGGCTGCGCATGGTGGTGGCGAGCTTCCTGTGCAAGG
ACCTGGGGCTGGACTGGCGCCGCGGCGAGCGCTATTTCGCTGAGAAGCTGAACGACTACGACCTCGCCGCCAACAACGGC
GGCTGGCAGTGGGCCAGCTCCAGCGGCTGCGACGCACAGCCCTACTTCCGCATCTTCAACCCGGTGAGCCAGAGCCAGAA
ATTCGACGCCCAGGGCAAGTTCATCCGCCGCTACCTGCCGCAGCTCGCCGCCCTGCCCGACGCCGCGCTGCACGCCCCGT
GGGACGCCAAGCCGCTCGACCTGGTCGCCGCCGGCCTGGTGCTCGGCCGCGACTACCCCGAGCCCATCGTGCGCCATGAG
CTGGCGCGCGAACGCACGCTGGCGCGCTATGCGGTGGTGAAGGGGGCGCGCTGA

Upstream 100 bases:

>100_bases
GTCGGCCATGCCGGGCATGGCAATCAGGAACTGATTCGTCAGGTTGGTGGCGGAGTCCGAGCCCATGCGGCGATTGTAGT
GACCGGGCACACTCCGGCGC

Downstream 100 bases:

>100_bases
GGCAGAGGCGACGCGGGAGGGCCCGCGCGCCCGAGGTCCGTCAACGCACGCCGAGGAACACCGTGCCGGCCGAGCGGTCG
GCGGATGTCAGGGCCACCAC

Product: deoxyribodipyrimidine photo-lyase type I

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MDKASGKTLDAALVWCRRDLRAHDHAALYHALRAARRVWVAFVFDTEILDPLPRTDRRVEFIRDSLVDLDAQLHTLGRSH
GTEGVGLIVRHGRARDEIPRLAAALHVQAVYANTDYEPAAVARDAQVRGALAQTGIAFHPAKDQVVFDTDEVLTGAGTPY
GVFTPYKNAWLKKLEPFYLQAYPVGRHAGALAPRPAGEAAGVPTLAAIGFEPTNLAALKLAPGTAGGRALLEDFGDERID
RYDQTRDFPAVKGPSYLSVHLRFGTLSIRELARLAWDRARAGSRGAEVWLSELIWRDFYHQVLHHHPQVVERSFKPEYDK
IKWEHGKHADALFAAWCEGRTGYPLVDAAMLQINQTGYMHNRLRMVVASFLCKDLGLDWRRGERYFAEKLNDYDLAANNG
GWQWASSSGCDAQPYFRIFNPVSQSQKFDAQGKFIRRYLPQLAALPDAALHAPWDAKPLDLVAAGLVLGRDYPEPIVRHE
LARERTLARYAVVKGAR

Sequences:

>Translated_497_residues
MDKASGKTLDAALVWCRRDLRAHDHAALYHALRAARRVWVAFVFDTEILDPLPRTDRRVEFIRDSLVDLDAQLHTLGRSH
GTEGVGLIVRHGRARDEIPRLAAALHVQAVYANTDYEPAAVARDAQVRGALAQTGIAFHPAKDQVVFDTDEVLTGAGTPY
GVFTPYKNAWLKKLEPFYLQAYPVGRHAGALAPRPAGEAAGVPTLAAIGFEPTNLAALKLAPGTAGGRALLEDFGDERID
RYDQTRDFPAVKGPSYLSVHLRFGTLSIRELARLAWDRARAGSRGAEVWLSELIWRDFYHQVLHHHPQVVERSFKPEYDK
IKWEHGKHADALFAAWCEGRTGYPLVDAAMLQINQTGYMHNRLRMVVASFLCKDLGLDWRRGERYFAEKLNDYDLAANNG
GWQWASSSGCDAQPYFRIFNPVSQSQKFDAQGKFIRRYLPQLAALPDAALHAPWDAKPLDLVAAGLVLGRDYPEPIVRHE
LARERTLARYAVVKGAR
>Mature_497_residues
MDKASGKTLDAALVWCRRDLRAHDHAALYHALRAARRVWVAFVFDTEILDPLPRTDRRVEFIRDSLVDLDAQLHTLGRSH
GTEGVGLIVRHGRARDEIPRLAAALHVQAVYANTDYEPAAVARDAQVRGALAQTGIAFHPAKDQVVFDTDEVLTGAGTPY
GVFTPYKNAWLKKLEPFYLQAYPVGRHAGALAPRPAGEAAGVPTLAAIGFEPTNLAALKLAPGTAGGRALLEDFGDERID
RYDQTRDFPAVKGPSYLSVHLRFGTLSIRELARLAWDRARAGSRGAEVWLSELIWRDFYHQVLHHHPQVVERSFKPEYDK
IKWEHGKHADALFAAWCEGRTGYPLVDAAMLQINQTGYMHNRLRMVVASFLCKDLGLDWRRGERYFAEKLNDYDLAANNG
GWQWASSSGCDAQPYFRIFNPVSQSQKFDAQGKFIRRYLPQLAALPDAALHAPWDAKPLDLVAAGLVLGRDYPEPIVRHE
LARERTLARYAVVKGAR

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=484, Percent_Identity=30.5785123966942, Blast_Score=205, Evalue=8e-53,
Organism=Homo sapiens, GI4758072, Length=495, Percent_Identity=28.0808080808081, Blast_Score=196, Evalue=6e-50,
Organism=Homo sapiens, GI188536103, Length=444, Percent_Identity=30.4054054054054, Blast_Score=185, Evalue=9e-47,
Organism=Escherichia coli, GI1786926, Length=499, Percent_Identity=39.6793587174349, Blast_Score=320, Evalue=2e-88,
Organism=Saccharomyces cerevisiae, GI6324962, Length=525, Percent_Identity=26.6666666666667, Blast_Score=182, Evalue=9e-47,
Organism=Drosophila melanogaster, GI17137248, Length=498, Percent_Identity=31.7269076305221, Blast_Score=211, Evalue=8e-55,
Organism=Drosophila melanogaster, GI24585455, Length=498, Percent_Identity=31.7269076305221, Blast_Score=211, Evalue=8e-55,
Organism=Drosophila melanogaster, GI24648152, Length=531, Percent_Identity=26.1770244821092, Blast_Score=150, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 55581; Mature: 55581

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKASGKTLDAALVWCRRDLRAHDHAALYHALRAARRVWVAFVFDTEILDPLPRTDRRVE
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEECHHHHCCCCCCHHHHH
FIRDSLVDLDAQLHTLGRSHGTEGVGLIVRHGRARDEIPRLAAALHVQAVYANTDYEPAA
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHEEEEECCCCCHHH
VARDAQVRGALAQTGIAFHPAKDQVVFDTDEVLTGAGTPYGVFTPYKNAWLKKLEPFYLQ
HHCCHHHHHHHHHCCCEECCCCCCEEECCHHHHHCCCCCCCCCCCCHHHHHHHCCCEEEE
AYPVGRHAGALAPRPAGEAAGVPTLAAIGFEPTNLAALKLAPGTAGGRALLEDFGDERID
EEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCHHHHHHHCHHHHH
RYDQTRDFPAVKGPSYLSVHLRFGTLSIRELARLAWDRARAGSRGAEVWLSELIWRDFYH
HHHHCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
QVLHHHPQVVERSFKPEYDKIKWEHGKHADALFAAWCEGRTGYPLVDAAMLQINQTGYMH
HHHHCCHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHH
NRLRMVVASFLCKDLGLDWRRGERYFAEKLNDYDLAANNGGWQWASSSGCDAQPYFRIFN
HHHHHHHHHHHHHHHCCCHHCCHHHHHHHCCCCEEEECCCCCEECCCCCCCCCCHHHHHC
PVSQSQKFDAQGKFIRRYLPQLAALPDAALHAPWDAKPLDLVAAGLVLGRDYPEPIVRHE
HHHHCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHH
LARERTLARYAVVKGAR
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDKASGKTLDAALVWCRRDLRAHDHAALYHALRAARRVWVAFVFDTEILDPLPRTDRRVE
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEECHHHHCCCCCCHHHHH
FIRDSLVDLDAQLHTLGRSHGTEGVGLIVRHGRARDEIPRLAAALHVQAVYANTDYEPAA
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHEEEEECCCCCHHH
VARDAQVRGALAQTGIAFHPAKDQVVFDTDEVLTGAGTPYGVFTPYKNAWLKKLEPFYLQ
HHCCHHHHHHHHHCCCEECCCCCCEEECCHHHHHCCCCCCCCCCCCHHHHHHHCCCEEEE
AYPVGRHAGALAPRPAGEAAGVPTLAAIGFEPTNLAALKLAPGTAGGRALLEDFGDERID
EEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCHHHHHHHCHHHHH
RYDQTRDFPAVKGPSYLSVHLRFGTLSIRELARLAWDRARAGSRGAEVWLSELIWRDFYH
HHHHCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
QVLHHHPQVVERSFKPEYDKIKWEHGKHADALFAAWCEGRTGYPLVDAAMLQINQTGYMH
HHHHCCHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHH
NRLRMVVASFLCKDLGLDWRRGERYFAEKLNDYDLAANNGGWQWASSSGCDAQPYFRIFN
HHHHHHHHHHHHHHHCCCHHCCHHHHHHHCCCCEEEECCCCCEECCCCCCCCCCHHHHHC
PVSQSQKFDAQGKFIRRYLPQLAALPDAALHAPWDAKPLDLVAAGLVLGRDYPEPIVRHE
HHHHCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHH
LARERTLARYAVVKGAR
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6325460; 8905232; 9278503; 2200511; 7604260 [H]